GENETIKA, Vol. 38, No.3 (2006)
Milka BRDAR, Borislav KOBILJSKI and Marija KRALJEVIĆ BALALIĆ
GRAIN FILLING PARAMETERS AND YIELD COMPONENTS IN WHEAT [Abstract] [Full text]
Nevena Mitić, Dejan Dodig and Radomirka Nikolić
VARIABILITY OF IN VITRO CULTURE RESPONSE IN WHEAT GENOTYPES, GENOTYPE AND ENVIRONMENTAL EFFECTS [Abstract] [Full text]
Milomirka MADIĆ, Desimir KNEZEVIĆ, Aleksandar PAUNOVIĆ and Nikola BOKAN
VARIABILITY AND INHERITANCE OF TILLERING IN BARLEY HYBRIDS [Abstract] [Full text]
Jovanka ATLAGIĆ and Sreten TERZIĆ
CYTOGENETIC STUDY OF HEXAPLOID SPECIES HELIANTHUS TUBEROSUS AND ITS F1 AND BC1F1 HYBRIDS WITH CULTIVATED SUNFLOWER, H. ANNUUS [Abstract] [Full text]
Goran Bekavac, Božana Purar, and Đorđe Jocković
GENETIC ANALYSIS OF DROUGHT ADAPTIVE TRAITS IN MAIZE SYNTHETIC POPULATIONS [Abstract] [Full text]
Dragana IGNJATOVIĆ-MICIĆ, Ana NIKOLIĆ, Snežana MLADENOVIĆ DRINIĆ, Jelena VANČETOVIĆ and Vesna LAZIĆ-JANČIĆ
IDENTIFICATION OF STERILE CYTOPLASM (CMS) IN MAIZE BY USING SPECIFIC mtDNA PRIMERS [Abstract] [Full text]
Vojka BABIĆ, M.BABIĆ and N. DELIĆ
STABILITY PARAMETERS OF COMMERIAL MAIZE (Zea mays L.) HYBRIDS [Abstract] [Full text]
Slađana JEVREMOVIĆ, Milana TRIFUNOVIĆ, Marija NIKOLIĆ, Angelina SUBOTIĆ and Ljiljana RADOJEVIĆ
CLONAL FIDELITY OF CHRYSANTHEMUM REGENERATED FROM LONG TERM CULTURES [Abstract] [Full text]
Olivera ILIĆ and Dragan ĐUKIĆ
CORELLATIONS AMONG ALFALFA YIELD COMPONENTS [Abstract] [Full text]
Sasa ORLOVIĆ, Andrej PILIPOVIĆ, Zoran GALIĆ, Petar IVANIŠEVIĆ and Nenad RADOSAVLJEVIĆ
RESULTS OF POPLAR CLONE TESTING IN FIELD EXPERIMENTS [Abstract] [Full text]
Published in „GENETIKA“ Vol. 38, No.3 (2006), pp. 175-181
© 2006 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade - Zemun
Serbia
UDC 575.22:633.11
Original scientific paper
GRAIN FILLING PARAMETERS AND YIELD COMPONENTS IN WHEAT
Milka BRDAR1, Borislav KOBILJSKI1 and Marija KRALJEVIĆ BALALIĆ2
1Institute of Field and Vegetable Crops, Novi Sad
2Faculty of Agriculture, Novi Sad
Abstract
Brdar M., B. Kobiljski and M. Kraljević Balalić (2006): Grain filling parameters and yield components in wheat. – Genetika, Vol.38, No. 3, 175-181.
Grain yield of wheat (Triticum aestivum L.) is influenced by number of grains per unit area and grain weight, which is result of grain filling duration and rate. The aim of the study was to investigate the relationships between grain filling parameters in 4 wheat genotypes of different earliness and yield components. Nonlinear regression estimated and observed parameters were analyzed. The rang of estimated corresponds to the rang of observed parameters. Stepwise MANOVA indicated that the final grain dry weight, rate and duration of grain filling were important parameters in differentiating among cultivar grain filling curves. The yield was positively correlated with number of grains/m2, grain weight and grain filling rate, and negatively correlated with grain filling duration. Correlation between grain weight and rate of grain filling was positive. Grain filling duration was negatively correlated with grain filling rate and number of grains/m2. The highest yield on three year average had medium late Mironovska 808, by the highest grain weight and grain filling rate and optimal number of grains/m2 and grain filling duration.
Key words: wheat, grain filling, yield components
Corresponding author: Milka Brdar, Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad,
e-mail: milkabrdar@yahoo.com
Published in „GENETIKA“ Vol. 38, No.3 (2006), pp. 183-192
© 2006 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade - Zemun
Serbia
UDC 575.22: 635.11
Original scientific paper
VARIABILITY OF IN VITRO CULTURE RESPONSE IN WHEAT GENOTYPES, GENOTYPE AND ENVIRONMENTAL EFFECTS
Nevena Mitić1, Dejan Dodig2 and Radomirka Nikolić1
1Institute for Biological Research "Sinisa Stankovic", Belgrade,
2Agriculture Research Institute "Serbia", Center for Agricultural and Technological Research, Zajecar, Serbia
Abstract
Mitić N., D. Dodig and R. Nikolić (2006): Variability of in vitro culture response in wheat genotypes, genotype and environmental effects. – Genetika, Vol. 38, No. 3, 183-192.
The tissue culture response (TCR) of immature embryos, evaluated according to callus formation, percentage of regenerative green-spotted calli and the number of plants per embryo, was investigated in 96 wheat genotypes of worldwide origins. Immature embryos were collected 12-15 DAP from field-grown plants during three successive years 2003, 2004 and 2005. Year 2003 was with high air temperatures and tropical days during a period of vegetation, while the environmental conditions were more favorable for plant growth in the next two years, 2004 and 2005. Embryos were cultured on standard MS medium containing 2 mg l-1 2,4-D. In all genotypes calli were efficiently induced, ranging from 36.7 to 100% (2003), 68.4 to 100% (2004), and 94.3 to 100% (2005). The calli occasionally formed green spots, but frequencies markedly differed among genotypes that varied from 0 to 72.5% (2003), 0 to 97.9% (2004), and 0 to 94.0% (2005). Coefficient of variation was highest in term of percent of regenerative calli (66.7%) following by a number of plants per embryo (35.6%) and callus formation (5.1%). Components of phenotypic variance showed that factor year (71.4%) had the highest impact on expression of callus formation, genetic factor (47.1%) on percentage of regenerative green-spotted calli and interaction year/genotype (30.3%) on number of plants per embryo. The results indicated factor genotype as the most important for determining regeneration potential in wheat.
Key words: immature embryos, callus formation, regenerating calli, plant regeneration, genotype, environmental condition, wheat
Corresponding author: Mitić Nevena, Institute for Biological Research "Sinisa Stankovic", Bul. Despota Stefana 142, 11000 Belgrade, Serbia
email: mitic.nevena@ibiss.bg.ac.yu
Published in „GENETIKA“ Vol. 38, No.3 (2006), pp. 193-202
© 2006 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade - Zemun
Serbia
UDC 575.3:633.16
Original scientific paper
VARIABILITY AND INHERITANCE OF TILLERING IN BARLEY HYBRIDS
Milomirka MADIĆ1, Desimir KNEZEVIĆ2 Aleksandar PAUNOVIĆ1 and Nikola BOKAN1
1Faculty of Agronomy, Čačak
2Faculty of Agriculture, Lešak
Abstract
Madić M., D.Knežević, A.Paunović, and N.Bokan (2006): Variability and inheritance of the tillering in barley hybrids.– Genetika, Vol. 38, No. 3, 193-202.
Productive tillering is a very important trait which influences the degree of barley lodging resistance. Five divergent genotypes of two-rowed winter barley (Vada, Djerdap, NS-293, Jagodinac and Sladoran) have been selected for diallel crossing in order to study the mode of inheritance, the gene effect and the components genetic variance for the productive tillering in F1 and F2 generation.
The test of the significance of the generations’ mean values related to the parental average was used for inheritance mode determinations (KRALJEVIC-BALALIC et al., 1991) and the genetic variance components and regression VrWr analysis from diallel crossings were made using the method of MATHER and JINKS (1971). The variability of the investigated trait differed. The cross combination Djerdap x Jagodinac had the highest coefficient of variance (39.78%). Different modes of inheritance (partial dominance, dominance and overdominance) and partial dominance were found in the F1 and F2 generation, respectively. The genetic variance components, average degree of dominance and regression line indicated partial dominance in the inheritance of tillering. The range of the obtained values of heritability for the tillering was found to be very wide (0.02-0.47). The cross combinations Djerdap x Jagodinac and Vada x Djerdap were selected for future breeding.
Key words: barley, tillering, variability, gene effect, genetic variance components
Corresponding author: Milomirka Madić, Faculty of Agronomy, Cara Dušana 34, 32000 Čačak, Serbia, Phone: +381 32 303 400; Fax: +381 32 345 264;
e-mail: mmadic@tfc.kg.ac.yu
Published in „GENETIKA“ Vol. 38, No.3 (2006), pp. 203-213
© 2006 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade - Zemun
Serbia
UDC 633: 575
Original scientific paper
CYTOGENETIC STUDY OF HEXAPLOID SPECIES HELIANTHUS TUBEROSUS AND ITS F1 AND BC1F1 HYBRIDS WITH CULTIVATED SUNFLOWER, H. ANNUUS
Jovanka ATLAGIĆ and Sreten TERZIĆ
Institute for Field and Vegetable Crops, Novi Sad, Serbia
Abstract
Atlagić J. and S.Terzić (2006): Cytogenetic study of hexaploid species Helianthus tuberosus and its F1 and BC1F1 hybrids with cultivated sunflower, H. annuus. – Genetika, Vol. 38, No. 3, 203-213.
Helianthus tuberosus is a potential source of resistance to many disease-provoking pathogens. Three accessions of H. tuberosus were used in this research and they were crossed with cultivated sunflower. Six F1 and two BC1F1 hybrid combinations were obtained. Analysis of meiosis was performed using aceto-carmine method (GEORGIEVA-TODOROVA, 1976) and pollen viability was determined by staining method of ALEXANDER (1969). Meiosis was regular in cultivated sunflower and the pollen viability was high (96.8-98.9%). Low percent of irregularities was found in the meiosis of H. tuberosus. Pollen viability was high (97.2-98.7%). Chromosome pairing was mostly regular in F1 hybrids (34 bivalents), but some meiocytes contained 28-32 bivalents with uni- and quadrivalents present. The percent of meiocytes with fast chromosomes in metaphase was 24.6-87.2, with lagging chromosomes in anaphase I 10.5-81.0 and in telophase 25.0-33.3. Chromosome bridges were detected in 0-9.9% of meiocytes in anaphase. Pollen viability in F1 hybrids ranged from 27.0 to 47.9%. In BC1F1 hybrids, number of bivalents was 16-25, univalents 2-18 and multivalents 0-1. Although a triploid set of chromosomes (51) was expected in BC1F1 hybrids, number of chromosomes was 45-57. Pollen viability varied from 0 to 54.3%.
Key words: Cultivated sunflower, Helianthus tuberosus, interspecific hybridization, meiosis, pollen viability.
Corresponding author: Altagić Jovanka, Institute for Field and Vegetable Crops, Maksima Gorkog 30, Novi Sad, Serbia. Phone: +381-21-489-8401
e-mail: atlagic@ifvcns.ns.ac.yu
Published in „GENETIKA“ Vol. 38, No.3 (2006), pp. 215-225
© 2006 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade - Zemun
Serbia
UDC 575.22: 633.15
Original scientific paper
GENETIC ANALYSIS OF DROUGHT ADAPTIVE TRAITS
IN MAIZE SYNTHETIC POPULATIONS
Goran Bekavac, Božana Purar, and Đorđe Jocković
Institute of Field and Vegetable Crops, Novi Sad, Serbia
Abstract
Bekavac G., B. Purar and Đ. Jocković (2006): Genetic analysis of drought adaptive traits in maize synthetic populations – Genetika, Vol. 38, No. 3, 215-225.
Maize breeders consider tolerance to drought as an important criterion in commercial breeding programs. Two traits, stay green and anthesis-silking interval seem to be closely associated with drought tolerance. The main objective of this study was to obtain estimates of means, variability, heritability and estimates of genetic correlations for several traits in two maize synthetic populations. S1 progenies were evaluated in an incomplete block design in four environments. Large genetic variability existed for all traits in both populations but highest variability was found for anthesis-silking interval. Strong, highly significant correlations between drought adaptive traits and grain moisture may cause some undesirable correlative response throughout cycles of selection.
Key words: maize, variability, correlation
Corresponding author: Goran Bekavac, Institute of Field and Vegetable Crops, M. Gorkog 30, 21000 Novi Sad, Serbia. Phone: +381-21-4898-251; Fax: +381-21-4898-300; e-mail: gbekavac@ifvcns.ns.ac.yu
Published in „GENETIKA“ Vol. 38, No.3 (2006), pp. 227-233
© 2006 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade - Zemun
Serbia
UDC 575; 633.15
Original scientific paper
Identification of sterile cytoplasm (cms) in maize by using specific mtDNA primers
Dragana IGNJATOVIĆ-MICIĆ, Ana NIKOLIĆ, Snežana MLADENOVIĆ DRINIĆ, Jelena VANČETOVIĆ and Vesna LAZIĆ-JANČIĆ
Maize Research Institute „Zemun Polje“, Belgrade, Serbia
Abstract
Ignjatović - Micić D., A. Nikolić, S. Mladenović Drinić, J. Vančetović, V. Lazić-Jančić (2006): Identification of sterile cytoplasm (CMS) in maize by using specific mt DNA primers – Genetika, Vol. 38, No. 3, 227-233.
Thirty sources of cytoplasmic male sterility (CMS) from Maize Gene Bank „Zemun Polje, distributed among Yugoslav OP varieties, have been tested for the presence of particular type of cytoplasm by a single seed multiplex PCR approach with specific primer pairs for T, C and S type cytoplasm. Combination of three pairs of primers in a single PCR reaction, corresponding to the chimeric regions of mtDNA sequences specific for each type of CMS, allowed reliable identification of the major CMS types. Dominant presence of S type cytoplasm was detected. For sources where there is no clear identification of the type of cms (absence of the PCR band) there is a reasonable doubt that it could be a new, yet unidentified type of cms.
Key words: CMS, maize, multiplex PCR
Corresponding author: Dragana Ignjatović-Micić, Maize Research Institute „Zemun Polje“, S. Bajića 1, 11185 Belgrade, Serbia, Phone: 011/3756-704, Fax: 011/3756-707
e-mail: idragana@mrizp.co.yu
Published in „GENETIKA“ Vol. 38, No.3 (2006), pp. 235-240
© 2006 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade - Zemun
Serbia
UDC 575.3.633.15
Original scientific paper
STABILITY PARAMETERS OF COMMERIAL MAIZE (Zea mays L.) HYBRIDS
Maize Research Institute „Zemun Polje“, S. Bajića 1, 11185 Belgrade, Serbia
Abstract
Babić V., M. Babić and N. Delić (2006): Stability parameters of commerial maize (Zea mays L.) hybrids – Genetika, Vol. 38, No. 3, 235-240.
Many authors showed the possibility of developing highly yielding and stable hybrids. The assumption was that commercial maize hybrids were characterised not only by the level of average yield bat also by their stability. Fifteen commercial maize hybrids of medium late and late FAO maturity groups (400-700) were used in the present study. The two-year four-replicate trials were set up in six locations according to the randomised complete-block design. Stability parameters were determined after the method of Eberhart and Russel (1966), in which the genotype was considered stable if it had a high average yield, a coefficient of regression of approximately 1.0 and deviation from regression as small as possible. The obtained results show that early maturity hybrids responded better to more modest growing condition and had a greater deviation from regression, while late maturity hybrids had a coefficient of regression closer to unity and they generally better responded to improved growing conditions.
Key words: stability parameters, commercial hybrids, maize
Corresponding author: Vojka Babic, Maize Research Institute, Zemun Polje, Slobodana Bajica 1, 11185 Belgrade-Zemun, Serbia. Phone: 381-11-3756-704;
E-mail: vbabic@mrizp.co.yu
Published in „GENETIKA“ Vol. 38, No.3 (2006), pp. 241-247
© 2006 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade - Zemun
Serbia
UDC 581165:635.9
Original scientific paper
CLONAL FIDELITY OF CHRYSANTHEMUM REGENERATED FROM LONG TERM CULTURES
Slađana JEVREMOVIĆ, Milana TRIFUNOVIĆ, Marija NIKOLIĆ, Angelina SUBOTIĆ and Ljiljana RADOJEVIĆ
Institute for Biological Research “Siniša Stanković”,Belgrade, Serbia
Abstract
Jevremović S., M.Trifunović, M. Nikolić, A. Subotić, and Lj.Radojević (2006): Clonal fidelity of chrysanthemum regenerated from long term cultures – Genetika, Vol. 38, No. 3, 241-247.
Morphological characteristics of flowers of long term regenerated chrysanthemum, cv. “White Spider”, after ten years of micropropagation are investigated. Shoot cultures are established and maintained more than ten years by stem segment culture on MS medium supplemented with BAP and NAA (1.0, 0.1 mgL-1, respectively). Rooting of shoots (100 %) has done on MS medium without hormones and it was very successfull after ten years, as well as, after two or eight years of micropropagation. Acclimation of rooted chrysanthemum plantlets at greenhouse conditions was excellent and after appropriate photoperiod “in vitro” plants flowered 90.3 % and have the same flower color, shape and size as mother plants. Flower color changes of “in vitro” plants are ooobserved during another flowering cycle one year after acclimatization. Observed variations of chrysanthemum flowers could be attributed to epigenetic factors.
Key words: Chrysanthemum morifolium, somaclonal variation, plant tissue culture.
Corresponding author: Slađana Jevremović, Institute for Biological Research “Siniša Stanković”, Bulevar despota Stefana 142, 11060 Belgrade, Serbia
Phone: +381 11 2078 425, Fax: +381 11 2761433
e-mail: sladja@ibiss.bg.ac.yu
Published in „GENETIKA“ Vol. 38, No.3 (2006), pp. 249-256
© 2006 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade - Zemun
Serbia
UDC 575.22:633.31
Original scientific paper
CORELLATIONS AMONG ALFALFA YIELD COMPONENTS
Olivera ILIĆ and Dragan ĐUKIĆ
Faculty of Agriculture, Novi Sad, Serbia
Abstract
Ilić O. and D. Đukić (2006): Corellations among alfalfa yield components – Genetika, Vol. 38, No. 3, 249-256.
Presented in this paper are the results of our study of correlations among yield components in ten alfalfa genotypes under open pollination (polycross) conditions. The study was conducted between 2000 and 2002 at the Rimski Sancevi Experiment Field of the Institute of Field and Vegetable Crops in Novi Sad. Highly significant correlations were found between pod number per inflorescence and seed number per inflorescence (r= 0.645) as well as between seed number per pod and seed number per inflorescence (r= 0.685). Similarly, the correlation between inflorescence number per stem and seed yield was significant and positive (r= 0.589). Negative correlations existed between ovule number per ovary on the one hand and seed yield and inflorescence number per stem on the other (r=- 0.617 and r=- 0.598, respectively).
The highest seed yield was achieved with the cultivar Europe (651.4 kg ha -1) and the lowest with the genotype Le-6 (426.25 kg ha -1). The difference was highly significant (CV= 14,93%).
Key words: alfalfa, genotypes, open pollination, genetic variability, correlations, seed yield.
Corresponding author: Olivera Ilić, Faculty of Agriculture, 21000 Novi Sad
Published in „GENETIKA“ Vol. 38, No.3 (2006), pp. 257-264
© 2006 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade - Zemun
Serbia
UDC 633: 575
Original scientific paper
RESULTS OF POPLAR CLONE TESTING IN FIELD EXPERIMENTS
Sasa ORLOVIĆ, Andrej PILIPOVIĆ, Zoran GALIĆ, Petar IVANIŠEVIĆ and Nenad RADOSAVLJEVIĆ
Institute of Lowland Forestry and Environment, Novi Sad, Serbia
Abstract
Orlović S., A.Pilipović, Z.Galić, P.Ivaniševič and N. Radosavljević (2006): Results of poplar clone testing in field experiments – Genetika, Vol. 38, No. 3, 257-264.
Growth vigour of 7 poplar clones was researched, i.e. 5 candidate clones for registration (B-81, B-229, PE 19/66, 182/81 and 129/8) and 2 clonal cultivars (I-214 and “Pannonia”) which had been registered earlier. The taxonomy of the study poplar clones was as follows: B-81 (Populus deltoides), B-229 (Populus deltoides), PE 19/66 (Populus deltoides), 182/81 (Populus deltoides), 129/81 (Populus x euramericana), I-214 (Populus x euramericana) and “Pannonia” (Populus x euramericana). The research was performed in three field experiments established in 2002 on the area of the Forest Estate Sremska Mitrovica. Although the experiments were established with several planting spaces, the first information indicate that the candidate clone PE 19/66 had the greatest vigour on the optimal soil types (humofluviosol and fluvisol loamy form), while the candidate clone B-81 had the advantage on the less favoured soil type (meadow black soil on loess alluvium). Based on the attained diameters and heights, it can be concluded that these are the successfully established plantations and that the candidate clones showed significant genetic potentials, which points to the fact that in future the production of poplar wood volume can be significantly increased.
Key words: Poplars, selection, growth vigour
Corresponding author: Saša Orlović, Institute of Lowland Forestry and Environment, Novi Sad