Nikolić Biljana, Milojević Nataša, Janković Ivana, Kolarević Stoimir, Jovanović Predrag, Jagodić Aleksandra, Vuković-Gačić Branka and Knežević-Vukčević Jelena

Antimutagenic potential of camphor, α/β thujone and eucalyptol in the bacterial assay system

Mitić-Ćulafić Dragana, Janković Milena, Ćakić Sanja, Vuković-Gačić Branka and Knežević-Vukčević Jelena

The effect of rat liver enzymes on antimutagenic potential of monoterpenes against t-booh-induced mutagenesis in E.coli

Mitić-Ćulafić Dragana, Žegura Bojana, Knežević-Vukčević Jelena and Filipič Metka

Evalution of antigenotoxic potential of linalool, eucalyptol and myrcene against IQ-and PhiP-induced DNA damage

Patenković Aleksandra, Stamenković-Radak Marina, Nikolić Dragana, Marković Tamara and Anđelković Marko

Antigenotoxic potential of tea infusion of Gentana lutea in wing spot test of Drosophila melanogaster

Topic III

Invited lecture

Milenković Ljiljana

Hedgehog signaling pathway in development and tumor formation

Introductory lectures

Novaković Ivana, Damnjanović T., Sango Violeta, Mladenović O., Maksimović N., Mirković Duško, Antonijević Nebojša, Simić-Ogrizović S., Sternic N. and Luković Ljiljana

Polymorphism C677T in the gene encoding for methylenetetrahydrofolate reductase in different vascular disorders

Oral presentations

Milićević Radomir, Branković Ljiljana, Radulović D., Jugović D., Stanković T., Stamenković H., and Kamenov B.

Cytogenetics-the present, the future and perspective

Todorić-Živanović Biljana, Strnad Milica, Stamatović Dragana, Grujović Jelena,Tanić Nikola, Ilić Vesnaand Magić Zvonko

Types of bcr-abl rearrangements in Serbian population and a level of expression in chronic myeloid leucemia patients

Lakić Nina, Krstić Aleksandra, Guć-Šćekić Marija, Kuzmanović Miloš, Mićić Dragan, Janjić Dragana, Konstantinidis Nadaand Kostić Gordana

Molecular diagnostic of chilodhood all

Strnad Milica, Brajušković Goran, Strelić Nataša, Todorić-Živanović Biljana, Stamatović Dragana and Magić Zvonko

Expression of BCL 2 protein and amplification of C-MYC gene in patients with chronic myeloid leukemia

Ćirković Sanja, Guć-Šćekić Marija, Pašić Srđan, Radivojević Danijelaand Jovanović Ankica

Results of cytogenetic and molecular testing in Serbian patients with Nijmegan breakage syndrome

Šupić Gordana, Kozomara Ružica, Branković-Magić Mirjana, Jović Nebojša and Magić Zvonko

Prognostic significance of tumor suppressor genes hypermethylation in oral squamous cell carcinoma

Đorđević Vesna, Denčić-Fekete Marija, Jovanović Jelica, Drakulić Danijela, Stevanović Milena, Janković Gradimir, Gotić Mirjana

Pattern of trisomy 1Q in hematological malignancies:a single institution experience

Jelenković Ankica

Genetics in pharmacology: an individualized medicine

Posters

Živanović Dušica, Šipetić Sandra,Stamenković-Radak Marina and Milašin Jelena

Family history of Graves-Basedow disease and environmental risk factors in etiology of this disease

Janakova Zorica

Population genetics in preclinical practice: presentation of 18 years work carried aut at genetic counselling offis of the medical practice in Jagodina

Popović-Kuzmanović Dragana, Novaković Ivana, Stojanović Ljudmila, Miković Danijela, Mirković Duško, Luković Ljiljanaand Aksentijević Ivona

Case study of possible familial antiphospholipid syndrome

Jelenković Ankica

Pharmacogenetics, drug interactions and adverse drug reactions

Cvetković Dragana, Cvetković Slobodan, and Novaković Ivana

Candidate gene polymorphisms and susceptibility to abdominal aortic aneurysm

Stojković Ljiljana, Živković Maja, Radak Đorđe, Kolaković Ana, Stančić Olja, Stanković Aleksandra and Alavantić Dragan

The association of IL-6 -174G/C gene polymorphism with carotid atherosclerosis in population of Serbia

Kolaković Ana, Stojković Ljiljana, Radak Đorđe, Đurić Tamara, Radak Sandra, Stanković Aleksandra, Alavantić Draganand Živković Maja

Matrix metaloproteinase-9 gene polymorphism -1562C/T as susceptibility factor for carotid atherosclerotic plaque development

Pejin Ivana, Buzadžić Ivana, Janković-Oreščanin Biljanaand Šuput-Tomaš Ljiljana

Distribution of paraoxonase 1 coding region polymorphisms in Serbian population

Đorđević Valentina, Nestorović Aleksandra, Tomić Branko, Miljić Predrag, Kovač Mirjana, Miković Danijela, Antonijević Nebojša, Rakićević Ljiljana and Radojković Dragica

The prevalence of FII A19911G and FII C20221T gene variants in thrombophilic Serbian population

Ilić Vesna, Ilić Miroljub and Magić Zvonko

Determination of ACE gene polymorphism distribution in diabetes nephropathy development

Đan Igor, Đan Mihajla, Obreht Dragana, Jovanović Nataša, Stokić Edita,Sakač Dejanand Vapa Ljiljana

Apoe gene polymorphism in patients with coronary heart disease

Buzadžić Ivana, Pejin Ivana, Ilić V. Tihomir, Janković-Oreščanin Biljana and Šuput-Tomaš Ljiljana

Association of Parkinson’s disease and polymorphism in environment responsive genes

Bošković Marija, Popović Branka and Milašin Jelena

Analysis of the -1562 C>T polymorphism in MMP-9 promoter in patients with head and neck carcinoma

Dobričić Valerija, Novaković Ivana and Kostić Vladimir

Determination of number of CAG repeats within HTT gene by capillary electrophoresis

Denčić-Fekete Marija, Đorđević Vesna, Tiziana Storlazzi Clelia, Janković Gradimir, Bogdanović Andrija, Jovanović Jelica, Rocchi Mariano, Todorić-Živanović Biljana, Strnad Milica and Gotić Mirjana

Translocation (5;6;12) associated with resistance to imatinib mesylate in chronic myeloid leukemia

Mišković Marijana, Lalić Tanja, Radivojević Danijela, Guć-Šćekić Marija, Zamurović Dragan and Vlahović Gordana

Prenatal and postnatal molecular study of SMA using restriction enzyme assay

Durković Jasmina, Cemerlic Dejan, Anđelić Luka and Drakulić Danijela

Survey of a case of gender reversion

Jovičić Dubravka, Rakić Boban, Vukov Tanja, Milačić Snežana, Pajić Jelena, Stevanović Milena and Drakulić Danijela

Comparative cytogenetic analysis of chromosomal aberrations and premature centromere division in lymphocytes of peripheral blood in persons professionally exposed to radionuclides

Daggag Hinda, White Stefan and Sinclair Andrew

Copy number changes in patients with disorders of sex development and functional analyses of candidate genes

Joksić Ivana, Guć-Šćekić Marija, Vujić Dragana, Petrović Sandra, and Leskovac Andreja

Telomere dysfunction induced foci correlates positively with incidence of sister chromatid exchanges in cells of fanconi anemia patients

Radivojevic Miloš, Keckarović-Marković Milica, Dacković Jelena, Apostolski Slobodan, Brajušković Goran and Romac Stanka

GLI3 gene molecular analysis of patients with Pallister-Hall syndrome

Lukić Jelena, Anđelković Marina and Bugarić Predrag

Rapid prenatal diagnosis of aneuploidy using quantitative fluorescence-PCR (QF-PCR) in Serbia

Popović Branka, Jekić Biljana, Novaković Ivanaand Milašin Jelena

The role of p53 gene and human papilloma virus in pathogenesis of oral squamous cell carcinomas (OSCCS)

Manasijević Maja, Nešić Branka, Jelovac Drago, Ilić Branislav, Popović Branka and Milašin Jelena

Analysis of c-myc and c-erbB-2 oncogenes in histopathologically tumour-free surgical margins in patients with oral squamous cell carcinoma and basal cell carcinoma

Fatović-Milićević Božica, Mitić-Ćulafić Dragana, Vuković-Gačić Branka, Berisavac Milica, Lakić Nada and Knežević-Vukčević Jelena

Detection of pre-manifested changes of cervix uteri with comet assay

Branković Ljiljana, Milićević Radomir, Živadinović Radomir and Milašin Jelena

Infections with human papilloma virus (HPV) and cytologic changes in the cervical epithelium

Topic IV

Invited lecture

Herrera J. Rene, Somarelli A. Jason, Mesa Annia, Rodriguez E. Carol

Do U1 small nuclear RNA variants mediate differential pre-mRNA splicing?

Introductory lectures

Kobiljski Borislav, Kondić-Špika Ankica, Trkulja Dragana and Brbaklić Ljiljana

Application of molecular markers in wheat breeding

Oral presentations

Golić Nataša, Terzić-Vidojević Amarela, Begović Jelena, Jovčić Branko, Veljović Katarina, Strahinić Ivana, Lozo Jelena, Nikolić Milica, Tolinački Maja and Topisirović Ljubiša

Molecular diversity among natural populations of Lactobacillus paracasei and Lactobacillus plantarum isolated from different ecological niches

Lozo Jelena, Kojić Milan, Jovčić Branko and Topisirović Ljubiša

Molecular characterization of bacteriocins produced by natural isolate Lactobacillus paracasei subsp. paracasei BGSJ2-8

Vojnović Sandra, Ilić-Tomić Tatjana and Vasiljević Branka

Secondary structure within 5’ untranslated region of the kgmB mRNA plantation establishment

Jovčić Branko, Fira Đorđe, Topisirović Ljubiša and Kojić Milan

Biodegradation of alkyl sulfates

Timotijević Gordana, Milisavljević Mira, Samardžić Jelena,Konstantinović Miroslav and Maksimović Vesna

Senescence-associated aspartic proteinase gene from buckwheat

Kalendar Ruslan

Universal amplification method of retrotransposon terminal ltr parts: new tool for polymorphism detection in plant and animal genomes and efficient retrotransposon cloning

Daggag Hinda, Svingen Terje, Western Patrick, van den Bergen Jocelyn, McClive Peter, Harley.Vincent, Koopman Peter and Sinclair Andrew

The Rhox homeobox gene family shows sexually dimorphic and dynamic expression during mouse embryonic gonad development

Mladenović Djordjević Aleksandra, Perović Milka, Smiljanić Kosara, Tešić Vesna, Tanić Nikola, Ruždijić Sabera and Kanazir Selma

Cholesterol homeostasis in the brain- regulation of gene expression in aging and caloric restriction

Tanic Miljana, Zajac Magdalena, Benitez Javier and Martinez-Delgado Beatriz

The role of microRNAs in regulation of BRCA1 gene

Posters

Bugarski-Stanojević Vanja, Stamenković Gorana, Adnađević Tanja, Blagojević Jelena, and Vujošević Mladen

The estimation of phylogenetic relationships among three species of genus Apodemus (Rodentia, Mammalia)

Tasić Srđan and Tasić Irena

Molecular characterisation of Seratia fonticola from oligomineral water

Tasić Srđan and Tasić Irena

Molecular characterisation of Pseudomonas sp. from oligomineral water

Djuric Simonida, Jarak Mirjana and Josic Dragana

RAPD fingerprinting of indigenous Azotobacter isolates from different types of soil

Jošić Dragana, Delić Dušica, Rasulić Nataša, Stajković Olivera, Kuzmanović Đorđe and Miličić Bogić

Phosphate solubilization and (GTG)5 analysis of pseudomonads from rhizosphere

Ivanović Žarko, Gavrilović Veljko, Živković Svetlanaand Stanković Slaviša

REP PCR as a method for determining diversitiy among Pseudomonas syringae strains from fruit trees

Lepšanović Zoricaand Libisch Balázs

Characterization of the metallo-b-lactamase gene from a Pseudomonas aeruginosa clinical isolate

Begović Jelena, Veljović Katarina, Strahinić Ivana and Topisirović Ljubiša

Antibiotic susceptibility of Staphylococcus epidermidis isolated from human milk storage

Jošić Dragana, Kuzmanović Slobodan, Stojanović Saša, Aleksić Goran and Starović Mira

XIIA group phytoplasma detection by RFLP analysis of 16S rDNA sequences

Tolinački Maja, Fira Đorđe, Kojić Milan and Topisirović Ljubiša

Antimicrobial activity of natural isolates of strain Lactobacillus paracasei subsp. paracasei

Veljović Katarina, Terzić-Vidojević Amarela and Topisirović Ljubiša

Antimicrobial and proteolytic activity of chosen Enterococci

Kočiš Tubić Nataša, Obreht Dragana, Pal Boža and Vapa Ljiljana

Microsatellite allelic variability in Ambrosia artemisiifolia L. populations in Vojvodina

Bratić Ana, Majić Dragana, Samardžić Jelena, and Maksimović Vesna

Functional promoter analysis of plant genes in bright yellow 2 cells and pollen grains

Aleksić Jelena, Geburek Thomas, Fussi Barbara and Heinze Berthold

Two mitochondrial group II introns in Picea omorika: New insights into evolutionary relations among spruces

Jevremović Slađana, Stanišić Mariana, Ćalić-Dragosavac Dušica, Raspor Martin, Ninković Slavica, Subotić Angelina, Trifunović Milana and Petrić Marija

Clonal fidelity of Iris sibirica plantlets regenerated by culture in vitro

Stevanović Jevrosima, Stanimirović Zoran and Radaković Milena

Investigations of genetic diversity of Paenibacillus larvae from Serbia using REP-PCR fingerprint technique

Kovačević Nives and Kaeppler Shawn

Chromatin mutants affecting paramutation of r1

Nešić Milica, Kuzmanović Dragana, Jovanović-Ćupić Snežana, Libek Vesna, Pašić Srđan and Stamenković Gorana

Standardisation of methods for detection of virus genome in blood and blood products: our experience with parvovirus B19

Savić Dejana, Kuljić Kapulica Nada and Jovanović Dragutin

The evaluation of PCR method for early detection and monitoring of citomegalovirus infection in transplanted patients

Meier Carste and Berenji Janoš

GM Arabidopsis thaliana for biodetection af landmines

Topic V

Invited lecture

Gömöry Dušan and Paule Ladislav

Traces of evolutionary processes in the gene pool of western-eurasian beeches

Heinze Berthold

Forest molecular genetics: promises, breakthroughs, and the practice

Introductory lectures

Janoš Berenji

New trends in plant breeding

Isajev Vasilije, Orlović Saša, Konstantinov Kosana, Drinić Mladenović Snežana, Lučić Aleksandarand Ivetić Vladan

The use of genetic markers in the enhancement of forest trees seed production in Serbia

Miščević Branislav, Bošković Jelena, Ivanc Aleksandar, Vukosav Marija and Kenjveš Tibor

Biotechnology and genetic improvement of domestic animals

Saftić-Panković Dejana, Radovanović Nataša, Jocić Siniša

MAS of sunflower for the resistance to downy mildew

Stevanović Jevrosima, Stanimirović Zoran, Ninoslav Đelić, Radaković Milena

Parentage verification and sex determination in cattle using molecular markers

Anđelković Violeta, Marković Ksenija, Ignjatović-Micić Dragana, Nikolić Ana, Drinić Mladenović Snežana and Lazić-Jančić Vesna

Maize breeding towards drought tolerance

Boćanski Jan and Savić Ivanov Milena

State and perspective of plant genetic resources in Serbia

KonstantinovKosana, Drinić Mladenović Snežana and IsajevVasilije

Crop, forestry and animal genetic resources – role of biotechnology for the characterization and conservation

Oral presentations

Dimitrijević Miodrag, Petrović Sofija, Banjac Borislav and Vukosavljev Mirjana

Phenotypic reaction of wheat grown on different soil types

Šurlan-Momirović Gordana, Zorić Miroslav, Kovačević Vlado, Prodanović Slaven, Vučković Savo, Maklenović Violeta, Jolanka Marton and Birkas Marta

Impact of global and regional climate changes on maize grain yield

Zečević Bogoljub, Đorđević Radiša, Damnjanović Jelena, Đorđević Mladen, Todorović Goran and Jevđović Radosav

Analysis of influence of parental germplasm on fruits characters of F1, F2 and F3 generations of pepper hybrids (Capsicum annuum L.)

Ellis Noel, Hofer Julie, Mikić Aleksandar, Mihailović Vojislav, Vasiljević Sanja, Milić Dragan, Đorđević Vuk and Perić Vesna

Leaf types in legumes and their agronomic importance

Smýkall Petrl, Kenicer Greg and Mikić Aleksandar

'Beautiful Vavilovia' (Vavilovia formosa) and molecular taxonomy of tribe Fabeae

Radičević Sanja, Cerović Radosav, Glišić Ivana and Karaklajić-Stajić Žaklina

Promising sour cherry hybrids (Prunus cerasus L.) developed at fruit research institute Čačak

Rakonjac Ljubinko, Isajev Vasilije and Lučić Aleksandar

Scots pine (Pinus silvestris L.) ecotypes of the Illyrian region in the afforestation of sites in south-eastern Serbia

Ivetić Vladan, Isajev Vasilije and Drinić Mladenović Snežana

Using of geographical component of genetic data

Galović Vladislava, Orlović Saša, Kovačević Branislav and Pilipović Andrej

Interspecific DNA variation in poplars using AFLP and SSR markers

Stevanović Jevrosima, Stanimirović Zoran and Radaković Milena

Species identification of Nosema microsporidian pathogen in samples of Apis mellifera from Serbia using PCR-RFLP method

Perović Dragan, Günter J., Steffenson B., Kopahnke D., Pržulj Novo and Ordon F.

Genetic mapping of leaf rust (Puccinia hordei Otth) resistance in barley accession MBR1012 originating from R. of Serbia and R. of Montenegro

Dodig Dejan, Kandić Vesna, Zorić Miroslav, Šurlan-Momirović Gordana and Knežević Desimir

The drought responce of wheat landraces from the Western Balkan

Nikolić Ana, Ignjatović-Micić Dragana, Lazić-Jančić Vesna and Pekić Sofija

QTL analysis of traits contributing to drought tolerance in maize

Dimitrieski Miroslav

Transmission of resistance to tobacco mosaic virus (TMV) in some oriental tobacco varieties

Denić Miloje, Ignjatović-Micić Dragana, Stanković Goran, Marković Ksenija, Žilić Slađana, Lazić-Jančić Vesna, Chauque Pedro, Fato Pedro, Mariote David, and Haag Wayne

Role of genetic resources of different geographic origin in simultaneous maize breeding for high protein quality and stress tolerance

Sokolović Dejan, Babić Snežana, Tomić Zorica, Radović Jasmina, Lugić Zoran and Cvetković Mirjana

Broadening of perennial ryegrass (Lolium perenne L.) and cocksfoot (Dactylis glomerata L.) autochthonous genotypes collection

Mratinić Evica, Fotirić Milica

Genetic resources of apple (Malus sp.) in south Serbia region

Longauer Roman, Lavadinović Vera, Gömöry Dušan Paule Ladislav, Borlea Gheorghe, Ballian Dalibor, Brus Robert, Zhelev Peta7 and Andonoski Aleksandar

Genetic variation of european silver fir (Abies alba) in southeastern Europe

Posters

Knežević Desimir Kovačević Vlado, Đukić Nevena, Jelić Miodrag and Luković Kristina

Genotypic variability of grain number per spike in wheat (Triticum aestivum L.)

Đukić Nevena, Knežević Desimir and Stamenković-Radak Marina

Variability of Gli-A2 alleles of 21 Triticum durum culltivars

Olivera Nikolić, Tomislav Živanović, Milovanović Milivoje

Possibilities of using physiological parameters in wheat breeding on grain yield

Petrović Sofija, Dimitrijević Miodrag, Banjac Borislav, Vukosavljev Mirjana

Spike stability parameters in wheat grown on solonetz soil

Jankuloski Ljupcho, Jankulovska Mirjana and Simeonovska Emilija

Induction of doubled haploids in Macedonian wheat genotypes (Triticum aestivum L.)

Prodanović Slaven, Girek Zdenka and Matzk Fritz

Effect of auxin concentration on the growth of wheat haploid embryos

Vukosavljev Mirjana, Kondić-Špika Ankica, Petrović Sofija, Dimitrijević Miodrag, Mladenov Velimir and Banjac Borislav

The effect of androgenic response components on regeneration wheat plants in vitro

Brdar-Jokanović Milka, Kobiljski Borislav, Kraljević-Balalić Marija and Maksimović Ivana

Wheat (Triticum aestivum L.) boron tolerance in field conditions

Kondić-Špika Ankica, Kobiljski Borislav, Marjanović Milica, and Hristov Nikola

In vitro evaluation of boron tolerance in wheat genotypes

Đurić Veselinka, Hristov Nikola, Kondić-Špika Ankica, Aćin Vladimir, Racić Marija

Effect of N mineral fertilization on the alveographic parameters of winter wheat

Jelić Miodrag., Đalović, I.., Knežević Desimir., Paunović Aleksandar

Variation of N, P and K contents in grain and straw of winter wheat genotypes in dependence of system of fertilization

Hristov Nikola, Mladenov Novica, Đurić Veselinka, Kondić-Špika Ankica, Marjanović-Jeromela Ana and Lečić Nada

Effect of fertilization on oil content in wheat grain

Gorjanović Biljana and Kraljević-Balalić Marija

The grain yield of bread wheat genotypes on three levels of nitrogen nutrition

Knežević Desimir, Zečević Veselinka, Đukić Nevena and Konstantinov Kosana

Genetic determination of technological quality of wheat (Triticum aestivum L.)

Zečević Veselinka, Knežević Desimir, Bošković Jelena, Mićanović Danicaand Luković Kristina

Variability of technological quality of winter wheat

Madić Milomirka, Paunović Aleksandar, Knežević Desimir and Zečević Veselinka

Inheritance of stem height and second internode length barley hybrids

Milovanović Milivoje and Perišić Vladimir

Results and perspectives of winter triticale breeding Kragujevac

Babić Vojka, Babić Milosav, Filipović Milomir and Delić Nenad

A visual estimation of the phenotype as an indicator of relatedness of maize inbred lines

Deletić Nebojša, Stojković Slaviša and Gudžić Slaviša

The change of genetic and phenotypic variability of yield components after recurrent selection of maize

Sečanski Mile, Živanović Tomislav, Šurlan-Momirović Gordana, Prodanović Slaven and Jovanović Snežana

Combining abilities for yield maize of lines from different election ciklus

Pajić Zorica, Erić Uroš, Srdić Jelena, Drinić Mladenović Snežana and Filipović Milomir

The mode of inheritance and heritability of the yield and the popping volume in popping maize (Zеа mays L. everta) hybrids

Ivanović Mile, Stanisavljević Dušan, Stojaković Milisav, Treskić Sanja and Mitrović Bojan

An evaluation of the two groups of half-sib families for grain yield in maize (Zea mays L.)

Srećkov Zorana, Nastasić Aleksandra, Ivanović Mile and Boćanski Jan

Correlation and path analysis of grain yield and morphological traits in test-cross populations of maize

Stojković Slaviša, Deletić Nebojša, Biberdžić Milan and Aksić Miroljub

The effect of yield components on grain yield in different progeny types of an F3 maize population

Pavlov Jovan, Stanković Goran and Delić Nenad

The utilisation of related maize inbreds in the hybrid seed production

Filipović Milomir, Čamdžija Zoran and Lopandić Dragiša

The performance of maize hybrids with exotic germplasm under conditions of our country

Babić Milosav, Babić Vojka, Prodanović Slaven and Anđelković Violeta

Yields stability of commercial maize hybrids

Cvarkovic Radomir, Branković Gordana, Calic Irena, Delić Nenad, Živanović Tomislav and Šurlan-Momirović Gordana

Stability of yield and yield components in maize hybrids

Đurović Dragan, Madić Milomirka and Stevović Vladeta

Stability parameters of grain yield in maize hybrids (Zea mays L.)

Vančetović Jelena, Jankuloski Ljupcho, Božinović Sofija and Dodig Dejan

The effects of cytoplasmic male sterility and xenia on the chemical composition of maize grain

Kravić Natalija, Anđelković Violeta, Hadži-Tašković Šukalović Vesna and Vuletić Mirjana

Antioxidant activity in seeds of maize genotypes with different percentage of exotic germplasm

Ristić Danijela, Ignjatović-Micić Dragana, Drinić Mladenović Snežana, Anđelković Violeta and Nikolić Ana

Comparison of RAPD and SSR markers for genetic diversity studies in maize local populations

Ignjatović-Micić Dragana, Marković Ksenija, Drinić Mladenović Snežana and Lazić-Jančić Vesna

SSR polymorphism assay for modifier loci in normal and opaque2 maize inbred lines

Kovačević Vlado and Šimić Domagoj

Heredity impacts on phosphorus and potassium concentrations in maize

Drinić Mladenović Snežana, Ristić Danijela, Sredojević Slobodanka, Dragičević Vesna, Terzić Dušanka, Nenad Delić and Ignjatović-Micić Dragana

Genetic variation of phytate in maize population

Radosavljević Milica, Mojović Ljiljana, Semenčenko Valentina and Milašinović Marija

The utility value of the grain of maize hybrids of a different genetic background

Atlagić Jovanka, Marjanović-Jeromela Ana, Marinković Radovan and Terzić Sreten

Pollen traits of the oil species from the Novi sad collection

Drumeva Miglena, Yankov Peter and Nenova Nina

New sunflower restorer lines developed by y-induced parthenogenesis from Helianthus annuus hybrids - disease resistance, combining ability. II. Combining ability.

Hladni Nada, Jocić Siniša, Miklič Vladimir, Saftić-Panković Dejana and Kraljević-Balalić Marija

Interdependence between yield and yield components of the confectionary sunflower

Marinković Radovan, Marjanović-Jeromela Ana, Radić Velimir

Genetics of some quantitative traits in sunflower (H. annuus L.)

Miladinović Dragana, Miladinović Jegor, Siniša Jocić and Miklič Vladimir

The effect of oxalic acid on asymmetry of somatic hybrids between cultivated sunflower and Helianthus maximiliani

Cvejić Sandra and Jocić Siniša

Development of hybrids of ornamental sunflower

Srebrić Mirjana, Kovačević Dragan and Perić Vesna

Relathionsip between conventional and kunitz free soybean lines in early generation selection

Perić Vesna, Nikolić Ana and Drinić Mladenović Snežana

Genetic diversity of soybean genotypes based on RAPD markers

Miladinović Jegor, Đorđević Vuk, Balešević-Tubić Svetlana and Miladinović Dragana

Genetic diversity of soybean genotypes originating from distant geographical regions

Perić Vesna, Srebrić Mirjana, Jankuloski Ljupcho, Mirjana Jankulovska, Žilić Slađana and Drinić Mladenović Snežana

The effects of nitrogen on protein and trypsin inhibitor content of soybean

Srebrić Mirjana, Perić Vesna, Dragičević Vesna, Sredojević Slobodanka, Terzić Dušanka and Drinić Mladenović Snežana

Genetic variability of phosphorius content in soybean seed

Marjanović-Jeromela Ana, Marinković Radovan, Miladinović Dragana, Stojaković Željka and Terzić Sreten

Inheritance of tocopherol contents in seeds of rapeseed (Brassica napus L.)

Danojević Dario, Kovačev Lazar, Ćurčić Živko, Nagl Nevena, Rajić Milorad and Stojaković Željka

Variability of root and seed traits in sugar beet

Terzić Sreten and Atlagić Jovanka

Nitrogen and sugar content variability in tubers of Jerusalem artichoke ( Helianthus tuberosus )

Korubin-Aleksoska Ana, Aleksoski Jane

Combining quantitative characters in breeding the lines of Nicotiana tabacum L. with diallel crossings

Miceska Gordana

Determination of the level of androgenesis in tobacco

Zdravković Jasmina, Pavlović Nenad, Girek Zdenka, Zdravković Milan and Cvikić Dejan

Characteristics important for organic breeding of vegetable crops

Kondić Svetlana, Takač Adam, Gvozdanović-Varga Jelica, Vasić Mirjana and Červenski Janko

Tomato ( Lycopersicum esculentum Mill. ) genotypes variability for the fruit traits

Živanović Tomislav, Prodanović Slaven, Šurlan-Momirović Gordana and Krstanović Saša

Genetic variability components of some quantitative traits of tomato

Pavlović Nenad, Cvikić Dejan, Zdravković Jasmina, Mijatović Mirjana and Brdar-Jokanović Milka

Dry matter content inheritance pattern in fresh bulb onion (Allium cepa L.)

Poštić Dobrivoj, Sabovljević Radovan and Bogdanović Zorica

Indicators viability of potatoe seed-tuber

Đorđević Radiša, Zečević Bogoljub, Damnjanović Jelena, Todorović Goran and Jevđović Radosav

Influence of af gene on the grain number of pea (Pisum sativum L.) per pod

Damnjanović Jelena, Zečević Bogoljub, Đorđević Radiša, Đorđević Mladen and Todorović Goran

Genetic analysis of inheriting the eggplant ( Solanum melongena L.) fruit widthh

Smýkall Petrl, Jarkovský J., Corander J. and Hýbl M.

Genetic diversity and population structure among pea (Pisum sativum L.) varieties revealed by combined marker analysis based upon retrotransposons, microsatellites and morphological characters

Mikić Aleksandar, Mihailović Vojislav, Ćupina Branko, Katić Slobodan, Vasiljević Sanja, Milić Dragan and Krstić Đorđe

Achievements in white lupin (Lupinus albus L.) breeding in Serbia

Radović Jasmina, Štrbanović Ratibor, Lugić Zoran, Sokolović Dejan and Vasić Tanja

Variability of morphological traits and dry matter yield of birdsfoot trefoil (Lotus corniculatus L.) wild populations

Mihailović Vojislav, Mikić Aleksandar, Dimitrijević Miodrag, Petrović Sofija and Katić Slobodan

Interspecies hybridisation in some annual legumes

Babić Snežana, Sokolović Dejan, Anđelković Snežana, Štrbanović Ratibor and Živković B.

Variability of the most important traits of timothy (Phleum pratense L.) autochthonous populations

Katić Slobodan, Milić Dragan, Mihailović Vojislav, Vasiljević Sanja and Mikić Aleksandar

Effects of heterosis for yield and yield components obtained by crossing divergent alfalfa populations

Lugić Zoran, Radović Jasmina, Sokolović Dejan, Anđelković Snežana, Babić Snežana

Genotype potential of different origins of white clover (Trifolium repens L.) for the basic agronomic features

Zec Gordan and Čolić Slavica

Influence of vineyard peach selection`s F1 generation to vigour and yield in peach autumn glo and nectarine stark red gold

Rakonjac Vera, Nikolić Dragan and Fotirić Milica

Genetic gain from selection of vineyard peach native population

Marić Slađana, Lukić Milan and Bošković Radovan

Polymorphism, inheritance and mapping of ETR1 gene in apple (Malus x domestica Borkh.)

Živković Svetlana, Stojanović Saša, Ivanović Žarko, Gavrilović Veljko and Balaž Jelica

Morphological and molecular analysis of Colletotrichum species from pear

Miletić Rade

Correlation between the onset of vegetation growth and major properties of walnut fruits

Miletić Rade

Correlation between the end of growing season and major fruit properties in a walnut population

Čolić Slavica, Milatović Dragan, Nikolić Dragan and Zec Gordan

Dehydrogenase isoenzyme polymorphism in selected almond genotypes (Prunus amygdalus Batsch)

Nikolić Mihailo, Radović Aleksandar, Fotirić Milica, Milivojević Jasminka and Nikolić Dragan

Pomological properties of promising raspberry seedlings with yellow fruits

Nikolić Dragan

Inheritance of some grapevine properties in crossing combination of seedling 113 x muscat hamburg

Šijačić-Nikolić Mirjana, Milovanović Jelena, Savić-Pavićević Dušanka, Brajušković Goran and Diklić Miloš

Sessile oak different regions genetic distance in Serbia

Šijačić-Nikolić Mirjana, Milovanović Jelena, Pilipović Andrej and Orlović Saša
Plants survive variability of different beech provenances within the serie of European provenance tests in Serbia

Lavadinović Vera, Isajev Vasilije and Radošević Gordana

Interdependence of width of needles and sites of douglas-fir provenances in test plantations in Serbia

Mrsic Marina and Pavlović P. Boro

Correlations of seed properties with its position inside seed cone of Abies alba Mill.

Lučić Aleksandar, Drinić Mladenović Snežana, Isajev Vasilije and Rakonjac Ljubinko

Polymorphism of seed proteins in populations of Scots pine (Pinus silvestris L.) in Serbia

Daničić Vanja, Isajev Vasilije, Mataruga Mila and Cvjetković Branislav

Inter-clonal variability of physiological properties of Scots pine (Pinus silvestris L) pollen

Ivetić Vladan

Use of GIS – from making conservation strategies to seed source selection

Andrić Andrijana, Obreht Dragana, Rat Milica, Pal Boža and Vujić Ante

Analysis of genetic variability in Ornithogalum L. using RAPD markers

Sedlovskiy А.I., Tupina L.N., Kоkhmetova А.М. and Baimagambetova К.К.

The screening of wheat germplasm to resistance to leaf rust in Kazakhstan

Prijić Željana and Jerković Zoran

Common to Puccinia triticina durable resistance genes combinations in Serbia

Staletić Mirjana, Milovanović Milivoje, Perišić Vladimir and Đekić Vera

Efficiency of Pc genes of resistance to crown rust of oat

Bekavac Goran, Purar Božana, Jocković Đorđe, Treskić Sanja, Stanisavljević Dušan and Mitrović Bojan

Genetic analysis for corn reddening tolerance in broad-base population NS 1-257 CRS

Radovanović Nataša, Jocić Siniša, Miklič Vladimir and Saftić-Panković Dejana

Changes in the Pl6 genomic region for the resistance to downy mildew in recombinant sunflower inbred lines

Mezei Snežana, Kovačev Lazar, Atlagić Jovanka and Nagl Nevena

Meiosis in diploid sugar beet populations rezistant to rhizomania

Živić Jelica, Knežević Desimir, Petrović Saša and Stančić Ivica

Variability of contents of nonsugar matter in rot of sugarbeet Beta vulgaris var. saccharifera at multigerme fertilizers in condition of rhizomania infection

Oro Violeta and Ivanović Žarko

Evaluation of DNA extraction methods in Globodera pallida PCR identification

Oro Violeta

Potato root pathocytologic changes and Globodera pallida (Nematoda: Heteroderidae) parasitism

Kovačević Branislav, Drekić Milan, Orlović Saša and Poljaković-Pajnik Leopold

Variability and relationship among characters of tolerance in black poplar clones to Phyllodecta vitellinae L.

Marković Ksenija, Kravić Natalija, Anđelković Violeta, Vuletić Mirjana

Response of maize genotypes with different proportion of exotic germplasm to osmotic stress

Simić Milena, Filipović Milomir, Dolijanović Željko, Grčić Nikola

The importance of genotypes for the increase of the competitive ability of maize against weeds

Dražić Slobodan, Dajić Zora and Živanović Tomislav

Diversity and utilization of medicinal plants genetic resources

Ilić Zoran, Milenković Lidija, Vasić Mirjana and Đurovka Mihal

Old cultivars and populations from traditional pepper-growing regions of Serbia

Taški-Ajduković Ksenija, Knoblauch Rainer, Nagl Nevena, Jevtić Aranka and Vujaković Milka

Genetic purity in seed testing using biomolecular markers

Sredojević Slobodanka, Dragičević Vesna, Srebrić Mirjana, Milivojević Marija and Đukanović Danica

The posibility of thermodynamic’s application in seed characterisation 1. Gibbs’ free energy.

Dragičević Vesna, Kobiljski Borislav, Radojčić Aleksandar, Dodig Dejan, Kandić Vesna, and Sredojević Slobodanka

The posibility of thermodynamic’s application in seed characterisation 2. Monocotyledon plants.

Nišavić Anika, Perić Vesna, Srebrić Mirjana, Đukanović Lana, Dragičević Vesnaand Sredojević Slobodanka

The posibility of thermodynamic’s application in seed characterisation 3. Dicotiledon plants.

Bošković Jelena, Isajev Vasilije, Miščević Branislav, Ivanc Aleksandar, Zečević Veselinka, Dozet Gordana

Interactions of gentically modified crops and environment

Milovanović Milivoje, Perišić Vladimir and Staletić Mirjana

Žarko – Perspective winter triticale cultivar

Perišić Vladimir, Milovanović Milivoje, Staletić Mirjana, Nikolić Olivera

Delija - New spring Triticale cultivar

ROUND TABLE I

Cvetković Dragana

Darwin’s legacy and modern genetics

Brockett Milosevic Mirjana

Strategies for teaching genetics and evolution more effectively

ROUND TABLE II

Marinkovic Dragoslav

Bioethics in science and medicine in R. Serbia

Magić Zvonko

Legislation of genetic testing in medicine

Konstantinov Kosana and Drinić Mladenović Snežana

Ethical aspects of genes and organisms protected by patent

Drinić Mladenović Snežana and Konstantinov Kosana

40 years of the journal Genetika

Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.6

FROM GENES TO GENOMES

Glišin Vladimir

Institute of Molecular Genetics and Genetic Engineering, Belgrade

The advent of our capabilities to sequence whole genomes of hundreds of organisms from bacteria to human, as well as to monitoring in a real time and in its totality the biochemical functionality of these genomes in different physiological, developmental, pathological, evolutionary settings, has significantly changed our tenet on molecular biology from the time of Watson’s Molecular Biology of the Gene (1965) to Molecular Biology of the Cell (2008). The old conventional wisdom said that to get a gene expressed, the DNA of that gene was copied into RNA, and that would be sent to the ribosome to be made into protein with the help of three RNAs. Now, everywhere you look there is some new kind of RNA with some known and unknown kind of function. Furthermore, there is a change how we view the genomes, particularly concerning how the genomes are assembled and how that information is translated to become proteins. Also, until recently, one supposed that structural variations within the genome of a species even within an individual, were not so common, but now there are more structural variations than we have ever expected. In my presentation I shall discuss and document the progress in the understanding of all biological processes based on genomics rather than on genetics.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.8

EVOLUTIONARY CONSERVATION GENETICS IN PRACTICE - IDENTIFICATION OF UNIQUE ENTITIES IN THE FAMILY Syrphidae

Milankov Vesna and Ludoški Jasmina

Department for biology and ecology, Faculty of Science,

University of Novi Sad, Novi Sad, Serbia

Delimitation of biological diversity is a crucial step in understanding phenomena in evolutionary biology, conservation biology, ecology, and biogeography. For identification of areas of genetic endemism of taxonomically diverse and challenging taxa, such as the family Syrphidae, it is important to delineate genetic units that are geographically discrete, often characterized by non-overlapping haplotype distributions and/or occurrence of unique allozyme markers. Identification of genetic uniqueness and genetic endemism emphasizes the importance of integration of multiple characters (mitochondrial sequence data, allozyme loci and quantitative traits) and estimation of genetic and phenotypic diversity in recognition of conservation units. Based on the observed private haplotypes, alleles and genotypes, unique heterozygotes, significant differences of allelic frequencies and recognized cryptic species across the full hierarchy of taxonomic and genetic diversity, molecular markers and wing geometric morphometrics are a suitable representation of the evolutionary potential of the hoverflies on the Balkan Peninsula.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.10

ARE B CHROMOSOMES GENOMIC PARASITES? – GEOMETRIC MORPHOMETRIC ANALYSIS OF THE CANALIZATION AND DEVELOPMENTAL STABILITY IN YELLOW-NECKED FIELD MICE

Apodemus flavicollis (Rodentia, Mammalia)

Jojić Šipetić Vida, Blagojević Jelena and Vujošević Mladen

Department for genetic research, Institute for biological research "Sinisa Stanković“, Belgrade, Serbia

The objective of this study was to test currently favored (parasitic) model of B chromosomes (Bs) maintenance. Bs, as true parasites, would increase level of stress in the host, i.e. B carriers would have lower levels of both canalization and developmental stability compared to non-carriers. To test this hypothesis 60 mandibles and 146 crania of adult yellow-necked field mice (Apodemus flavicollis) collected during one year on Mt. Jastrebac were analyzed. Size and shape variance among individuals and fluctuating asymmetry (FA) were estimated using geometric morphometric methods. B carriers had a higher variance for cranium shape, although without statistically significant difference. Non-carriers showed significantly higher level of FA for cranium shape compared to B carriers. The fact that B chromosomes do not decrease the levels of canalization and developmental stability of analyzed skull features in studied population of A. flavicollis should not be considered as a conclusive argument for accepting some of the alternative models of Bs maintenance, but only as the evidence for rejecting herein tested parasitic model.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.11

MICROSATELLITE ANALYSIS OF GENETIC DIVERSITY IN WILD BOAR

Veličković Nevena¹, Đan Mihajla¹, Obreht Dragana¹, Zorić Miroslav²and Vapa Ljiljana¹

¹Department for biology and ecology, Faculty of Science,University of Novi Sad

²Facultyof agriculture, Belgrade, Serbia

The aim of this study was the assessment of genetic diversity in the captive wild boar population from Danube region. A total of 51 muscle tissue samples were collected. A set of five microsatellite markers, developed for domestic pig, was used. Amplification was successful at 4 of them, and all four loci were included in statistical analysis since linkage disequilibrium test showed that loci were not associated. A total number of 59 alleles were found, with an average of 14.7 alleles/locus. The highest number of alleles per locus was found at S0068 microsatellite (24), while the lowest number (9) was present at locus SW251. Group-specific numbers of allele was higher in young (55) then in adults (26). Among all alleles, 22 (37.29%) were found in both analyzed groups. All others were private. Average observed heterozygosity (Ho) values for population was 0.579 (0.348-0.833). Similar values were found for both groups. All F_IS values were significantly higher then zero, which reflects a substantial deficit of heterozygotes.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.12

LARGE-SCALE DIFFERENTIATION OF WILD BOAR (Sus scrofa) POPULATIONS: IS THERE INTRASPECIFIC STRUCTURE?

Paule Ladislav¹, Bakan Jana¹, Kuehn Ralph², Nikolov Ivan², Romšáková Ivana¹

and Krajmerová Diana¹

¹Faculty of Forestry, Technical University, Zvolen, Slovakia

²Technische Universität München, Freising, Germany

Wild boar (Sus scrofa) distribution covers the entire continental Europe; it is missing in British Isles, Scandinavia and northern part of the European Russia. The intraspecific taxonomy recognizes numerous subspecies, while in central and south-eastern Europe five subspecies should occur: S.s. scrofa, S.s. atilla, S.s. lybicus, S.s. meridionalis and S. s. algira. Fourteen microsatellites of nuclear DNA composed in four multiplexes: (1) S008, SW986, SW1129; (2) SW1701, SW828, SW1517; (3) SW1465, SW1492, SW1514, SW2532; (4) SW461, SW841, SW2021, SW2496 were used to study the genetic structure and differentiation of the central and south-eastern European wild boar populations and to search for links with the occurrence of individual subspecies. Sharp differentiation of wild boar populations was between Slovakia and the Czech Republic and also between the southern and northern Bulgarian populations, which might indicate that in Western Slovakia and Bulgaria are suture zones of genetic lineages.

The paper was financially supported by research grant APVV-18-032105.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.13

GENETIC fragmentation OF Rupicapra rupicapra balcanica POPULATIONS ALONG THE BALKAN PENINSULA

Paule Ladislav¹, Grubešić Marijan², Krajmerová Diana¹, Tomljanović Kristijan², Ballian Dalibor³ and Zhelev Petar⁴

¹ Faculty of Forestry, Technical University, Zvolen, Slovakia;²Šumarski fakultet, Zagreb, Croatia;³ Šumarski fakultet, Sarajevo, Bosnia and Herzegovina;⁴ University of Forestry, Sofia, Bulgaria

Distribution range of R.r. balcanica stretches from Slovenia to Greece and at present it is discontinuous. We have studied the genetic diversity and differentiation of chamois populations along the Balkan Peninsula with the aim to find the degree of admixture of chamois of Alpine origin (R.r. rupicapra) in Western Balkan. Nuclear microsatellites according to Muntwyler et al. (2002) were used for characterization of genetic diversity and differentiation of individual subpopulations and to define the mixed populations. Northern Croatian populations from Risnjak and Kupa proved to belong to Alpine genetic type together with Slovenian populations. Populations from Biokovo in Croatia and Devin in Bulgaria form another genetic group while Velebit populations with several Bosnian individuals contained both genetic types. Consequences for gene conservation and population management are given.

The paper was financially supported by research grant APVV-18-032105.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.14

“SEX RATIO IN Drosophila pseudoobscura: EVOLUTIONARY ANALYSIS

OF SR POLYMORPHISM”

Brockett Milosevic Mirjana and Anderson W. Wyatt*

School of Biology, Georgia Institute of Technology, Atlanta, GA, USA

*Department of Genetics, University of Georgia, Athens, GA, USA

Sex ratio (SR) condition has been described in the Drosophila literature as a case of almost entirely female offspring produced by certain wild females. In this study, we have focused on the SR trait in D. pseudoobscura, a species from Western coast of the United States and Canada. In this species the SR chromosome is manifested with three inversions on the right arm of X chromosome. SR chromosome never reaches fixation in nature, while despite some selective disadvantages, SR inversions remain widespread within the genus. We have tested some models for selective maintenance of the Sex ratio polymorphism, both in laboratory and natural populations. Our results indicate that in D. pseudoobscura there seems to be frequency dependent gamete selection, responsible for maintenance of the SR genotypes.

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Book of Abstratcts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.15

“GEOGRAPHICAL CHANGES IN RELATIVE FREQUENCY OF INVERSIONS IN CHROMOSOME III OF Drosophila pseudoobscura AMONG NATURAL POPULATIONS FROM MEXICO”

Salceda M. Víctor

Departamento de Biología. Instituto Nacional de Investigaciones Nucleares, Carretera México-Toluca S/N, La Marquesa, Ocoyoacac, Mexico

Chromosomal polymorphism in natural populations of Drosophila pseudoobscura have been broadly studied in the USA but scarcely in Mexico with only about 60 localities analyzed. Differences among both regions are notorious with respect to their chromosomal constitution. Northern populations, those of USA, have as representative inversions the sequences ST, AR and CH contrasting with those in Southern populations (Mexico) in which prevail the gene arrangements TL, CU and SC. Assuming as a probable mechanism that has allowed these substitutions the flow generated by the presence of a North-South clines, we took as a goal find out if such clines really exist. With that objective in mind we studied 29 populations of this species distributed along four North-South transects. Specimens of D. pseudoobscura caught by attracting them with fermenting bananas, carried to the laboratory where from each female an isofemale line was established. When their offspring appeared a single larva from each isofemale was taken, its salivary glands extracted and stained with a solution of lacto-aceto-orcein, by these means the polytene chromosomes were obtained. On these chromosomes we identified, for each larva, the inversion (s) carried in the third chromosome, in such a way 3439 third chromosomes were analyzed. Among the 29 localities we identified 17 different inversions but the number of them varied from population to population from three to eleven. Relative frequencies of each inversion at every location were calculated and with them for each transect the presence or absence of clines was determined. Among each transect the existence of clines was observed only between two or three near by populations, but we were not able to find a clear manifestation of the presence of clines along a complete transect. Our results at this respect are similar to those previously reported for USA populations. A mechanism that explains North-South substitutions of predominant inversions remains as open question.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.16

CONSEQUENCES OF OUTBREEDING ON DEVELOPMENTAL STABILITY IN Drosophila subobscura WINGS

Kurbalija Zorana¹, Stamenković-Radak Marina^1,2 and Anđelković Marko^1,2

¹Institute for biological research "Sinisa Stanković“, Belgrade, University of Belgrade, Belgrade; ² Faculty of Biology, University of Belgrade, Belgrade

Fluctuating asymmetry (FA), the increased variation of bilateral symmetry in a sample of individuals, can point to disturbance in developmental stability at molecular, chromosomal and epigenetic level. The aim was to study different genetic causes of FA, such as interpopulation hybridization as genomic stress and find out weather co-adaptations or heterozygosity are probable mechanisms which maintain developmental homeostasis in populations. We focused on co-adaptive aspect of inversion polymorphism in Drosophila subobscura and it’s relation with genomic stress, such as interpopulation hybridization and developmental stability. We analysed the effect of interpopulation hybridization through the variability of fluctuating asymmetry (FA) of wing size parameters (wing width and wing length) of D.subobscura from three ecollogically distinct populations, presuming that they possess a certain degree of genetic differentiation due to their different evolutionary histories. The FA analysis and statistics was done on samples from P, F1 and F2 generations of inter- and intrapopulation crossings.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.17

PRODUCTION OF ANTIMICROBIAL SUBSTANCES AND GENETIC DIVERSITY OF Bacillus sp. STRAINS ISOLATED FROM DIFFERENT HABITATS IN SERBIA

Berić Tanja¹, Fira Đorđe², Stanković Slaviša¹, Urdaci C. Maria³

and Knežević-Vukčević Jelena¹

¹Faculty of Biology,Department of microbiology, University of Belgrade;²Faculty of Biology,Department for biochemistry and molecular biology, University of Belgrade , Serbia;³Labaratory for microbiology and biochemistry application, ENITA de Bordeaux, Gradignan, France

In this study, genetic diversity and production of antimicrobial substances (AMS) of 192 Bacillus strains isolated from different geographical and ecological niches in Serbia was studied. Combining RAPD analysis and 16S DNA sequencing we determined thirteen different groups of RAPD profiles within four species. B. pumilus was represented with three groups of different RAPD profiles, B. subtilis with five, B. cereus with four and B. firmus with one. Production of AMS was tested against plant pathogens: Burkholderia glumae, B. cepacia, Erwinia carotovora, Pseudomonas fuscovaginae and Xanthomonas oryzae. Out of 192 strains, 128 showed some degree of antimicrobial activity against at least one indicator strain, and 42 strains exhibited effect on three and more pathogens.No apparent association between RAPD profiles and the geographic origin or habitat of the strains tested was detected. Antimicrobial activity was related to species and RAPD group. Most AMS producers including the highly potent ones belonged to B. subtilis species.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.20

DISTRIBUTION OF B CHROMOSOMES IN AGE CATEGORIES OF YELLOW-NECKED MOUSE APODEMUS FLAVICOLLIS (MAMMALIA, RODENTIA)

Vujošević Mladen, Blagojević Jelena, Jojić Šipetić Vida, Bugarski-Stanojević Vanja, Adnađević Tanja and Stamenković Gorana

Department for genetic research, Institute for biological research "Sinisa Stanković“, Belgrade, Serbia

Supernumerary chromosomes, found in many species, are named B chromosomes (Bs) if they satisfy three criteria: dispensability, absence of paring with A chromosomes and non-Mendelian inheritance. Bs are frequently found in populations of yellow-necked mice, Apodemus flavicollis. Maintenance of Bs in populations is explained by two opposed models. Model signed parasitic claims that Bs are maintained by mechanism of accumulation despite detrimental effects on carrier fitness. On the other hand, heterotic model suggests that, in the absence of accumulation mechanism, small number of Bs could confer an adaptive advantage to carriers, while large number could be deleterious. With the aim of contributing to understanding the mechanisms of Bs maintenance in populations of A. flavicollis, differences in frequencies of Bs in age categories were analysed in 732 animals. Bs described as parasites reduce mean fitness of individuals carrying them. Therefore, it is expected that frequency of animals with Bs will decrease with age increase. Absence of significant statistical differences in frequency of Bs between six age categories point to the heterotic way of Bs maintenance in A. flavicollis.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.21

GENOTYPING OF FRUŠKA GORA POPULATION OF YELLOW-NECKED MOUSE APODEMUS FLAVICOLLIS (RODENTIA, MAMMALIA) – AFLP AND B CHROMOSOMES

Adnađević Tanja, Bugarski-Stanojević Vanja, Stamenković Gorana,

Blagojević Jelena and Vujošević Mladen

Department for genetic research, Institute for biological research "Sinisa Stanković“, Belgrade, Serbia

B chromosomes (Bs) are extra chromosomes to the standard complement that occur in many organisms. Because of high degree of homology with A chromosome, Bs of Apodemus flavicollis can not be accurately distinguished from chromosomes of normal complement at cytogenetic level, but there are differences at molecular level. AFLP is PCR-based technique that is useful for the rapid screening of genetic diversity. It generates hundreds of highly replicable markers, thus allowing high-resolution genotyping. We investigated usefulness of AFLP markers for genotyping mice in the context of Bs presence or absence. Total of 75 individuals were included in the study and 16 of them are B carriers, all captured on Mt. Fruška Gora. Out of 22, 14 primer combinations gave clear and reproducible pattern. Presence or absence of DNA fragments were transformed to binary data (1, 0). The computing of binary data was performed using MVSP software and genetic distances were estimated by Nei & Li's coefficient and UPGMA clustering. High level of detected intrapopulation polymorphism makes AFLP markers useful tool for genotyping analysis.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.22

ANALYSIS OF HYPERVARIABLE DOMAIN 1 OF CONTROL REGION mtDNA IN BROWN HARE POPULATIONS (Lepus europaeus pallas)

Đan Mihajla¹, Obreht Dragana¹, Tanurdžić Miloš², Vapa Milan³ and Vapa Ljiljana¹

¹Faculty of Sciences, Dept of Biology and Ecology, Novi Sad; ²Cold Spring Harbor Lab, New York, USA; ³Faculty of Agriculture, Novi Sad, Serbia

Hypervariable domain 1 of control region of mtDNA is known molecular marker in population genetics studies of Lepus. The aim of this research is to analyse mtDNA CR-1 sequence variation in brown hares from Serbia. In total 157 hares from six regions (Backa, Banat, Srem, Eastern, Central and Western Serbia) were analysed. After sequencing of mtDNA CR-1, subset of 92 sequences (426-432bp) was used for further analyses. Among those 45 haplotypes were found. Unique haplotypes were detected in each population. The highest number of unique haplotypes was present in Central Serbia (9). Haplotype diversity for all populations was Hd=0.944, and nucleotide diversity was π=0.0236. Based on number of nucleotide differencies (Kxy) and genetic divergence (Fst) NJ dendograms were constructed. Both dendograms showed similar branch topology, indicating some separation between two groups of populations, first consists of three populations from Vojvodina and second consists of three populations from Eastern, Central and Western Serbia. The most genetically divergent population was the Western Serbia with two unique sequences detected for the first time in L. europaeus.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.23

OPTIMISATION AND STANDARDIZATION OF PRIMERS FOR STERLET (Acipenser ruthenus) AND BELUGA (Huso huso) MICROSATELLITE LOCI

Cvijanović Gorčin¹, Adnađević Tanja², Bugarski-Stanojević Vanja²

and Lenhardt Mirjana¹

¹ Department of Natural Resources and Environmental Sciences, Institute for Multidisciplinary Research, Belgrade, Serbia;² Department for genetic research, Institute for biological research "Sinisa Stanković“, Belgrade, Serbia

Overharvest, habitat loss, fragmentation and other anthropogenic factors have resulted in population and distribution decline of both sterlet (Acipenser ruthenus) and beluga (Huso huso) in Danube basen. As all living sturgeon species are endangered and there are numerous conservation programs, there is a need for estimation of genetic diversity and the evolutonary relationships among populations. Microsatellite loci are useful tool for detection of intraspecies and intrapopulatoin polymorphism and numerous microsatellite loci were detected for sturgeon species. Primers for loci from Afu, Aox and Spl groupe were preveously developed for lake sturgeon (Acipenser fulvescens), Atlantic sturgeon (Acipenser oxyrinchus oxyrinchus) and shovelnose sturgeon (Scaphirhynchus platorynchus) respectfuly and provided a much needed group of genetic markers, detectable with non-invasive sampling (anal fin tissue). We tested and optimised primers for ten microsatellite loci on 10 sterlet and 5 beluga speciments from Serbian part of Danube River. All of tested loci show high level of polymorphism among sample speciments.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.24

GENETIC POLYMORPHISM OF SALICORNIA EUROPAEA GROUP (Chenopodiaceae)

Polić Dubravka, Veselić Sanja, Đan Mihajla and Obreht Dragana

Department of Biology and Ecology, University of Novi Sad,

Faculty of Sciences, Novi Sad, Serbia

In order to estimate the genetic polymorphism of the Salicornia, seven different RAPD (Random Amplified Polymorphic DNA) primers were assayed for four isolated populations from different European regions (Saladar de Aquamarga -Spain, Slano Kopovo -Serbia, Ulcinj salina- Montenegro and Kalloni salina- Lesvos). The screened RAPD primers yielded 58 polymorphic bands: five for primer OPB11, seven for OPB06 and OPB12, eight for K01 and K15, ten for OPA01 and 15 for M02. Values of pairwise comparisons of Nei and Li genetic distance between populations ranged from D=0.469 (Serbia-Spain) to D=0.750 (Serbia-Montenegro). The study indicated that populations from Spain and Serbia are closely related with each other while Montenegro group is different from the both Serbia and Spain groups, which led to conclusion that the Montenegro group belongs to other population which might derived from an ancestry different from that of the Spain and Serbia population. On the other hand, the study pointed out that Lesvos group is quite different from all the other investigated populations.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.25

PRELIMINARY RESULTS OF MOLECULAR GENETIC ANALYSES OF THE GENUS Branchipus schaeffer 1766 (Crustacea, Branchiopoda)

Miličić Dragana, Petrov Brigita, Stojković Biljana and Pavković-Lučić Sofija

Institute of Zoology, Faculty of Biology, University of Belgrade,

Belgrade, Serbia

To gain insights into the taxonomic relationships among different Branchipus populations, molecular genetic analyses were performed. Data matrices used in the analyses included 629 base pairs from the mitochondrial gene COI. We used specimens from Pannonian populations (both Hungarian and Serbian), as well as from localities in Eastern Serbia. Samples of Branchipus intermedius (from Macedonia) were used as outgroup. Both the NJ tree based on p-distances and the MP tree on maximum parsimony were calculated. Results from both methods indicated major divergence between two lineages: the lineage from Pannonian populations, and Eastern Serbia lineage. These preliminary results suggest significant evolutionary branching of the “southern” lineage (from Eastern Serbia), and, consequently, possible higher taxonomic level of this population group. Further analyses are needed for reaching consistent conclusion.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.26

MATING SUCCESS AND WING MORPHOLOGY OF Drosophila subobscura FROM ECOLOGICALLY DIFFERENT ENVIRONMENTS

Savić Tatjana¹, Stamenković-Radak Marina^1,2, Savić Marija², Kenig Bojan¹

and Anđelković Marko^1,2

¹Department for genetic research, Institute for biological research "Sinisa Stanković“, Belgrade, Serbia;² Faculty of Biology, University of Belgrade, Belgrade, Serbia

Drosophila species exhibit genetic and phenotipic variation in traits affecting mate choice. This variation forms the basis for sexual selection that may lead to isolation between populations in ecologically different environment. Mating success of the flies in different Drosophila species depends on a variety of factors including male courtship vigour, female receptivity, male size and other signals emitted during the courtship.Ecological speciation is commonly considered in terms of pre-mating isolation. In context of this, morphological variation of wing size and shape in ecologically different environments may cause intraspecific variation.Drosophila subobscura provide excellent model system to compare frequency of homo- and heterogametic maiting among populations in divergent environments. Mate preference functions and wing morphology can further be used to develop predictions of relative mating success in population hybridizations.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.27

A LACK OF GENETIC VARIATION IS ASSOCATED WITH ASYMMETRY OF WING TRAITS IN NORTHERN HOVERFLY SPECIES (Diptera, Syrphidae)

Milankov Vesna¹, Ludoški Jasmina¹, Francuski Lubinka¹, Ståhls Gunilla2 and Vujić Ante¹

¹Department for biology and ecology, Faculty of Science,University of Novi Sad, Novi Sad, Serbia;²Finnish Museum of Natural History, University of Helsinki, Finland

The molecular and phenotypic diversity of the Cheilosia aff. longula and C. naruska species (Diptera, Syrphidae) from Lapland, Finland, were examined through DNA sequencing, protein electrophoresis and geometric morphometrics. The morphological identification of the species was verified molecularly using partial sequences of mitochondrial COI and the nuclear ribosomal ITS2 genes comparing with corresponding sequences of previously published of C. aff. longula and closely related C. longula as well as corresponding sequences of a paratype of C. naruska. Analysis of isozyme loci showed an extremely low genetic variability in the studied populations. Variation among genetically diverse individuals was studied both within- and among studied populations, and directional (DA) and fluctuating asymmetry (FA) were estimated using landmarks in the framework of geometric morphometric methods. The documented DA and FA asymmetry in both wing shape and size likely reflected developmental instability of studied individuals. The observed consistent association between high asymmetry of wing traits and low heterozygosity would indicate a reduced potential of populations for adaptive evolution.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.28

CHROMOSOMAL INVERSION POLYMORPHISM IN Drosophila subobscura POPULATION FROM THE GORGE OF THE DERVENTA RIVER

(TARA MOUNTAIN)

Jelić Mihailo¹, Kurbalija Zorana², Kenig Bojan², Stamenković-Radak Marina^1,2

and Anđelković Marko^1,2

¹Faculty of Biology, University of Belgrade, Belgrade; ²Institute for biological research "Sinisa Stanković“, Belgrade, University of Belgrade, Belgrade,Serbia

Due to geomorphological characteristics and climatic conditions, gorges are considered to have been refugia during last glaciation. Therefore, the aim of this research was to determine wheter genetic variability of Drosophila subobscura population from the gorge of the Derventa river (Tara Mountain) shows some local distinctivnes. What makes the Tara National Park especially valuable is the fact that this area is a refugium with complex forest phytocoenoses in which endemics and relicts dominate and have been preserved to date. Habitat conditions on Tara have not significantly changed since the Tertiary to date. Inversion polymorphism is suitable for this survey since it responds to some extent to climatic change.Gene arrangement frequencies of D. subobscura population from the Derventa river gorge were compared to the ones determined before for the other populations in Serbia. No endemic arrangements were found in the analyzed population, except those characteristic for the Balkan Peninsula refugium, in general. High dispersion capacity of D. subobscura may explain lack of genetic distinctiveness in inversion polymorphism which probably existed in this refugium.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.29

CHROMOSOME NUMBER VARIATION IN SEXUALLY DIMORPHIC SPECIES Mercurialis perennis L. (Euphorbiaceae) FROM SERBIA

Jovanović Vladimir¹, Blagojević Jelena², Vujošević Mladen²and Cvetković Dragana¹

¹Faculty of Biology, Universityof Belgrade, Belgrade, Serbia; ²Department for genetic research, Institute for biological research "Sinisa Stanković“, Belgrade, Serbia

Evolutionary transitions between various sexual systems in plants have attracted a great deal of research. Processes leading to change in chromosome number were important in shaping evolution of genus Mercurialis, that shows exceptional variation in sexual system, as well as extensive chromosomal variation. Previous studies revealed that polyploidy was associated with transition from dioecy to hermaphroditism in M. annua. Its congeneric, M. perennis, is almost exclusively dioecious species with wide latitudinal and altitudinal distribution. It represents the extreme example of chromosome variation – it consists of polyploid and aneuploid complex (2n= 42–112); about 43 different cytotypes have been described so far, most with basic number x=8. While populations from certain parts of European range are well studied in this respect, cytogenetic data from southeastern part is missing. The aim of this study was to examine the variation in chromosome number, i.e. presence of polyploid and aneuploid complex in M. perennis populations in Serbia. Karyological analyses of samples from low altitude populations did not confirm the presence of extreme variation – the observed chromosome numbers fall within the previously reported range, with 2n=60 being the most common variant.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.30

THE EFFECTS OF POLLUTION IN POPULATIONS OF FIELD MOUSE,

Apodemus agrarius – HAEVY METALS AND DEVELOPMENTAL STABILITY

Blagojević Jelena¹, Jojić Šipetić Vida¹, Jovanović Vladimir²and Vujošević Mladen¹

¹Department for genetic research, Institute for biological research "Sinisa Stanković“, Belgrade, Serbia;²Research station Petnica, Valjevo

Concentration of heavy metals was assessed in bone tissues of widely distributed species of field striped mouse, Apodemus agrarius (Rodentia, Mammalia) from two populations in the locations with different levels of pollution, mountain Cer and Pančevo. Comparison of concentrations of eight heavy metals (Zn, Ni, Co, Mn, Cu, Cd, Pb, Fe) was done between the localities, sex and age categories. The effect of pollution was clear for the most of heavy metals studied. Differences between sexes were found for iron and copper in samples from Pančevo. Bioaccumulation of all heavy metals, except zinc, is increased in the youngest age category on mountain Cer. Using the method of geometric morphometry, the same samples of animals were analysed for the level of fluctuating asymmetry (FA) for mandible and cranium. FA is the measure of developmental stability and is often used for assessing the effects of environmental factors on living systems. Comparison of obtained results and concentrations of heavy metals was done and discussed.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.31

THE INFLUENCE OF ELECTROMAGNETIC FIELDS ON FITNESS COMPONENTS DURING DEVELOPMENT OF Drosophila subobscura

Patenković Aleksandra¹, Savić Tatjana¹and Anđelković Marko^1,2

¹ Institute for biological research "Sinisa Stanković“, Belgrade, Serbia

² Faculty of Biology, University of Belgrade, Belgrade, Serbia

Extremely low frequency (ELF) electromagnetic field (EMF) is increasingly present abiotic environmental factor. Studies of the effect of EMF have been performed on a variety of species from different aspect, but it is still obscure how organisms respond and/or adapt to such environmental change. Electromagnetic field used in this experiment was 0.25mT and of extremely low frequency of 50Hz. This intensity and frequency is common for EMF which are widely used at workplace and home. The effect of exposure to this EMF was observed through fitness components during three generations in species Drosophila subobscura. The females from different inbred lines laid eggs in the presence or absence of EMF for 24 h period, and the progeny is reared in identical conditions for an additional 24 h. Therefore, the progeny was exposed to EMF in embrional and early postembrional stages of development. In both groups, egg-to-adult viability, early fecundity and development time was analyzed. Changes in fitness components during several generations reveal that ELF - EMF is a physical factor wich affects embrional development.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.32

DEVELOPMENTAL INSTABILITY IN Drosophila subobscura IN CONDITIONS OF DIFFERENT DEGREE OF LEAD POLLUTION

Kenig Bojan¹, Kurbalija Zorana¹, Stamenković-Radak Marina^1,2 and Anđelković Marko^1,2

¹ Institute for biological research "Sinisa Stanković“, Belgrade, Serbia

² Faculty of Biology, University of Belgrade, Belgrade, Serbia

Heavy metal pollution of natural ecosystems is one of the negative consequences of human activity. Lead is one of the most widespread pollutants. Adaptation to toxic substances in the environment can lead to the increased resistance and changes in genetic structure of the exposed populations. Drastic environmental deterioration presents environmental stress which can affect various phenotypic traits, such as developmental homeostasis of organisms. Disturbance of developmental homeostasis, developmental instability (DI), is a suite of processes that tend to disrupt precise development in particular environment. Variation in bilateral traits, such as fluctuating asymmetry (FA), is in a direct correlation with developmental instability, environmental changes and stress. The aim of this study was evaluation of FA level in laboratory populations of D. subobscura exposed to different concentrations of lead. Variation in wing-shape asymmetry in D. subobscura was analyzed by method of geometric morphometrics. In that manner, the relationship between environmental stress caused by lead with DI levels during seven successive generations was analysed.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.33

Effect of previous mating experience on some components

of mating behavior in Drosophila melanogaster

Pavković-Lučić Sofija, Kekić Vladimir and Miličić Dragana

Institute of Zoology, Faculty of Biology, University of Belgrade, Belgrade, Serbia

Sexual behavior in Drosophila melanogaster is very complex: it includes series of various courtship steps, followed by an interchange of different types of stimuli, like olfactory, acoustic, visual and tactile. However, many aspects of the reproductive behavior of D. melanogaster may be modified by experience and age. It is well known that in D. melanogaster, females "decide" if and with which male mating will take place. These "decisions" definitely depend on various sensory cues from the males, which they use in demonstration their attractiveness. We supposed that female mating experience also may contribute to mate choice.According to our results, larger males were more succesfull in mating with “experienced” females. Also, females with previous mating experience had significantly slower mating speed than virgin females, while, on the other side, virgin females copulated significantly longer than previously mated ones. Opposite to females, males with or without previous mating experience, did not differ in mean mating speed and mean duration of copulation.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.34

Fluctuating asymmetry and sexual selection

in Drosophila melanogaster

Pavković-Lučić Sofija and Kekić Vladimir

Institute of Zoology, Faculty of Biology, University of Belgrade, Belgrade, Serbia

Fluctuating asymmetry (FA) is defined as small, random departures from bilateral symmetry, which is ’’ideal state’’ of bilateral traits; it reflects levels of genomic and environmental stresses during development. Relationship between sexual selection and FA is very complex and, still controversial.In a series of experiments (two in laboratory conditions and one in the field), we investigated if more symmetrical D. melanogaster males were more succesfull in mating. In the laboratory experiment I, males were developed at different growth temperatures (18 ºC vs. 25 ºC), while, in laboratory experiment II, males were reared on one out of three different substrates for more than 35 generations (standard cornmeal medium, banana and tomato).Contrary to results obtained in laboratory, in field experiment, male mating success was associated with low FA. Namely, non-mating males showed elevated FA both in wing length and number of sex comb teeth.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.35

Sexual selection and mating pattern related to body size in three Drosophila species in natural conditions

Kekić Vladimir and Pavković-Lučić Sofija

Institute of Zoology, Faculty of Biology, University of Belgrade, Belgrade, Serbia

Sexual selection is recognized as a special form of natural selection and defined as selection that arises from differences in mating success. Large body size in males emerged as a correlate of mating success across a range of insect taxa, including Drosophila. However, investigations with a number of Drosophila species have indicated that as more Drosophila species were studied, the number of examples where body size and male mating success were uncorrelated was increasing. The goals of the present study were: (i) test for differences in mean body size between mating and non mating (control group) of flies from natural populations of three Drosophila species (D. melanogaster, D. hydei and D. immigrans), (ii) test for mating pattern related to body size. Mating behavior of those species were investigated during two seasons, at two different localities (Sremska Kamenica, Bačko Gradište, Serbia). Body size was correlated with mating success only in D. melanogaster. Size-assortative mating was observed only in D. hydei and only in one season. Different results obtained in these species would be discussed in a course of their different reproductive strategies.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.36

FLUCTUATING ASYMMETRY AND COMPONENTS OF FITNESS IN COMPETITIVE AND NON- COMPETITIVE CONDITIONS IN Drosophila subobscura

Savić Marija¹, Savić Tatjana², Pavković-Lučić Sofija¹, Stamenković-Radak Marina^1,2

and Anđelković Marko^1,2

¹ University of Belgrade, Faculty of Biology, Belgrade, Serbia.

² University of Belgrade, Institute of Biological Research, Belgrade, Serbia.

It has been suggested that fluctua. Some studies failed to report this, but many have reported association between fluctuating asymmetry of bilateral morphological traits and individual fitness traits. This relationship can be explained by sexual selection, if levels of asymmetry influence mate choice, or by natural selection, if increased level of FA are associated with reduced performance for life history traits. We used Drosophila subobscura in the conditions of competitive and non competitive mating, comparing fluctuating asymmetry and components of fitness. The present study focused on the fluctuating variation, of wing size and ovariole number in females and wing size and sex combs number in males, and association with early female fecundity and egg-to-adult viability. The results suggest that individual FA is poor indicator of fitness components and the generality of the relation between developmental instability and fitness remains a controversy. ting asymmetry (FA), as a measure for developmental instability, is associated with fitness

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.37

REPRESENTATION OF SPECIATION OF THE OUTCOME AMONG SPECIALISTS VS. GENERALISTS IN RELATION TO HABITAT IN THE LIMNOFAUNA OF EUROPE

Pavlović Nevenka and Pavlović P. Boro

Faculty of Natural Sciences and Mathematics, Banja Luka

The relations between species and sub-species with types of habitat have been analysed in the frame of the limnofauna of Europe. The register encompasses 15384 species and sub-species, and 53 types of habitat. For 12464 species and sub-species there is information on a linkage with one to nine (1-9) types of habitat grouped in 607 variations, what makes 19038 relations.A scale of the generalists is defined in the span ranging from 0 (zero) for the species using only one type of habitat, to eight (8) for the species appearing in nine (8) types of habitat.The use of the two types of habitat is a transitional phase towards specialisation or increased generalisation in occupation of more types of habitat.High diversity in 53 types of habitat appears in the interval of the scale of generalists ranging from 0.38 to 1.43, while low diversity is present in a larger number of types of habitat in the remaining area of the scale of generalists.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.40

GENERAL OVERVIEW OF THE COMET ASSAY

Anderson Diana

University of Bradford, Bradford, BD7 1DP, UK

The Comet assay has become one of the most important assays in toxicology of the last decade or so. It is now a standard method for measuring DNA strand breaks in single cells. Cells embedded in agarose on a microscope slide are lysed with detergent and high salt. Electrophoresis at high pHs results in structures resembling comets, observed by fluorescence microscopy. The comet tail is formed by DNA fragments moving towards the anode. The assay can be used for genotoxicity testing of novel compounds and ecotoxins, human biomonitoring and molecular epidemiology and basic research into DNA damage and repair. DNA repair can be measured after treatment of cells with a DNA damaging agent and measuring damage remaining after different time intervals. This overview will be illustrated with examples from the work of the author’s group and collaborators. These will include for genotoxicity testing the specificity and sensitivity of the assay, for ecotoxins the work with DBP halogenated acetic acids, for human biomonitoring and molecular epidemiology the work with mother and babies, diabetes and lead-exposed children and for fundamental research that with drug resistant Non-Hodgkin’s lymphoma patients over-expressing p53 mutant protein and lacking DNA repair. Most of this work has been carried out in somatic human lymphocytes, but it can also be carried out in germ cells as human sperm. Using sperm, the oestrogens can cause DNA damage which can be diminished with anti-oxidants suggesting an oxygen radical involvement. Also as age increases in men so there is an increase in DNA damage.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.41

GENOTOXIC EFFECTS OF ORGANOPHOSPHORUS PESTICIDES

Filipič Metka and Hreljac Irena

National Institute of Biology, Ljubljana, Slovenia

Chronic exposure to organophosphorous compounds (OPs) has been linked to the development of different cancers. With human hepatoma HepG2 cells we showed that methyl-parathion (PT) and methyl-paraoxon (PO) induced DNA damage, whereas dimefox (DF) induced increased cell proliferation. PT and PO upregulated mRNA expression of DNA damage responsive genes: p53, p21, GADD45α and MDM2, while DF upregulated only expression of p53. We further found synergistic effects of PT and PO on BaP induced mutagenesis in the bacterial reverse mutation assay, and micronucleus assay in HepG2 cells. We demonstrated for the first time that the underlying mechanism of synergistic effect in HepG2 cells was PT and PO mediated modulation of the metabolic activation of BaP: OPs suppress activity and expression of cytochrome 1A1 (CYP1A1) and favours the induction of aldo-keto reductase 1C (AKR1C) that catalyse the formation of DNA reactive BaP o-quinones and the production of reactive oxygen species. Our results indicate that different types of OPs affect cancer development by different mechanisms including mitogenic effect, direct genotoxicity and synergistic effects in combination with PAH.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.44

GENETIC MODELS IN INVESTIGATION OF MOLECULAR MECHANISMS OF GENOTOXICITY AND ANTIGENOTOXICITY

Vuković-Gačić Branka, Simić Draga and Knežević-Vukčević Jelena

Department for microbiology, Faculty of Biology, University of Belgrade, Belgrade, Serbia

DNA repair mechanisms, which are involved in preserving the integrity of the genome, are largely conserved among prokaryotes and eukaryotes, including human cells. Human XPC protein detects distortion in DNA helix in much the same way as bacterial UvrA, and XPD possess helicase activity similar to bacterial UvrB. Eukaryotic cells also repair mismatches with homologs of MutS and MutL, and genetic predisposition to colon cancer (HNCC) is due to mutations in corresponding MSH and MLH genes. Moreover, human RAD51, a homolog of the bacterial RecA protein, promotes homologous paring and DNA strand exchange. The modulation of DNA repair, and increase or decrease of mutagenic effect, can be monitored in different genetic backgrounds. Genetic endpoints such as spontaneous and induced mutations, recombination, and mutagenic repair can be studied with microbial short-term tests, and addition of mammalian enzymes provides valuable information about genotoxicity or antigenotoxicity in mammals. Our study of antigenotoxic compounds from medicinal and aromatic plants with microbial tests will be presented.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.45

WHAT IS KNOWN AND WHAT IS UNKNOWN IN RADIATION PROTECTION

Joksić Gordana

Institute of nuclear science „Vinča“

This paper summarize contribution of radiobiology in the field of radiation protection in the last 3 decades. The most important discoveries and findings are accounted chronologically and are discussed with respect to protection against of ionizing radiation.

The three decades of intensive research in radiation biology didn’t gave an clear answer on the basic question in biodosimetry- dose-response relationship at low doses of ionizing radiation, in another word a contribution of low doses in neoplastic transformation. Although is known that particular types of ionizing radiation differs significantly in their ability to induce DNA damages, mechanisms underlying this phenomenon are still unclear. Ionizing radiation at doses that are significant for radiation protection behaves as weak carcinogen, whereas irradiation with doses above 500 mSv induce erythema, cataract, infertility. It is known that the incidence of the most primarily induced events gave linear relationship with absorbed dose, which is highly surprising knowing that the multilevel nature of the most biological processes. Although current research didn’t gave a final answers to many questions, it is obvious that ionizing radiation induce complex interactions among many molecules and processes in cells Novel methods, developed for studying of DNA are of importance for radiation protection, and will be discussed in this paper.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.48

ANTIOXIDANTS REDUCE DNA DAMAGE INDUCED BY ADRENALINE

IN THE COMET ASSAY

Radaković Milena¹, Đelić Ninoslav¹and Plećaš-Solarović Bosiljka²,

Spremo-Potparević Biljana², Živković Lada²and Bajić Vladan³

¹Department for biology, Faculty of veterinary medicine, University of Belgrade

²Institute for physiology, Faculty of pharmacy, University of Belgrade

³Institute for research and development, Department for biomedical research, Galenika, Belgrade, Serbia

Catechol groups can be involved in redox cycling accompanied by generation of reactive oxygen species (ROS) which may lead to covalent damage of cellular macromolecules including DNA. In this work we investigated level of primary DNA damage in isolated human lymphocytes exposed to adrenaline. DNA damage was evaluated by the in vitro Comet assay (single cell gel electrophoresis). Firstly, we examined dose-response relationship after the treatment with a broad spectrum of adrenaline concentrations (range from 0.0005 μM to 500 μM) and we found out that with the increase of the adrenaline dose there is an increase of DNA migration. At the concentration of 500 μM we observed the most profound genotoxic effects and acceptable level of cytotoxicity (less than 25%, determined by Trypan blue exclusion test). Therefore, we chose this concentration for further examination with antioxidants catalase and quercetin. It has been observed that catalase (100 IU and 500 IU) and quercetin (100 μM and 500 μM) significantly reduced quanitity of DNA in comet tails. Therefore, it can be concluded that adrenalin exerts genotoxic effects mainly through induction of reactive oxygen species (ROS).

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.49

GENOTOXIC PROPERTIES OF ELECTRIC ARC FURNACE DUST ON HUMAN LYMPHOCYTES: IN VITRO STUDY

Garaj-Vrhovac Vera and Gajski Goran

Institute for Medical Research and Occupational Health, Mutagenesis Unit, Zagreb, Croatia

One of the global problems currently recognised worldwide, is a rising exposure of general population to small amounts of leachates of electric arc furnace dust (EAFD), which can pollute large volumes of ground and surface waters.The purpose of this work was to test the alkaline comet assay as a biomarker of the genotoxicity of EAFD leachates, established prior/after the alkaline extraction process. Prior and following the treatment, lymphocytes were incubated with leachate of EAFD for 6 and 24 hours. Results showed that EAFD leachates induced comet assay-detectable DNA damage in human lymphocytes compared to the control. The same goes for the samples exposed to EAFD leacheates pre-treated with a strong alkaline solution. Our data indicate that the ever-increasing amounts of mixture of a number of toxic chemicals, originated from various waste materials residual to a variety of technological processes, such as EAFD, may be responsible for the DNA damage, and suggest that the exposure of human population to such leachates may lead to adverse healthy effects. In addition, comet assay prove to be sensitive when assessing genotoxicity of various waste materials, such as EAFD leachates.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.50

CHEMICAL, CYTOTOXIC AND GENOTOXIC PROPERTIES OF WASTEWATER FROM METAL INDUSTRY

Durgo Ksenija^a, Kopjar Nevenka^b, Franekić Čolić Jasna^a, Šulić Krsta^c, Ćurčić Marijana^d and Antonijević Biljana^d

^a Faculty of Food Technology and Biotechnology, Zagreb, Croatia;^b Institute for Medical Research and Occupational Health, Zagreb, Croatia;^c Public Health Institute, Požarevac, Serbia;^d Institute of Toxicology academic Danilo Soldatović, Faculty of Pharmacy, Belgrade, Serbia

In many countries there is a growing problem concerning environmental pollution from industrial wastewaters. Majority of wastewaters that are released into environment satisfy law regulative on limit values of hazardous substances in wastewater, but mixture of compounds can cause deleterious effects on biological system. In this study we investigated chemical composition and genotoxic effects of wastewater collected from five locations of the region of metal industry. For determination of chemical composition of wastewater we used the method of atomic absorption spectrophotometry (AAS). Mutagenic potential of wastewater was determined on Salmonella typhimurium strains. Cytotoxic effect and primary DNA damage of wastewater was measured on human laryngeal and cervical carcinoma cells. Compounds present in five wastewater samples do not exceed permissible limits. Nevertheless, higher concentrations of wastewaters caused cytotoxic effect on both cell lines, but HeLa cell line was more responsive to lower concentrations of wastewater. All samples caused primary DNA damage in dose-responsive manner. Based on results obtained, additional treatment of the wastewater prior to discharge is recommended.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.52

INFLUENCE OF ANTIOXIDANT ENZYMES ON THE RADIOBIOLOGICAL RESPONSE OF FANCONI ANEMIA LYMPHOCYTES IN VITRO

Petrović Sandra¹, Vujić Dragana², Guć-Šćekić Marija², Joksić Ivana³,

Leskovac Andreja¹, Joksić Gordana¹

¹Institute of nuclear science „Vinča“;²Mother and Child Health Care Institute of Serbia “Dr. Vukan Čupić“, Belgrade;³Gynecology and Obstetrics Clinic „Narodni front“, Belgrade, Serbia

Fanconi anemia (FA) is genetic disease characterized by progressive pancytopenia and cancer susceptibility. The clinical and cellular phenotypes of Fanconi anemia are associated with a redox abnormalities indicating that FA is oxidative stress related disorder. One of the features of FA cells that remain unclear is their response to ionizing radiation. The aim of this study was to evaluate the in vitro radiosensitivity of FA homozygotes and heterozygotes, and to assess the contribution of antioxidant enzymes to the overall radiobiological response of the cells. FA patients exhibited a significant decrease in catalase activity, accompanied with lower activity of SOD and increased incidence of baseline micronuclei compared to control. The most of patients displayed a radioresistant response accompanied with enhanced percentage of radiation-induced apoptosis. Activity of catalase and percentage of apoptotic cells correlated inversely. Enhanced apoptosis also was observed in heterozygote-parents and was accompanied with lower activity of catalase in carrier-mothers. The results of study suggest that reduced activity of catalase is an important contributor to the radiobiological response of cells.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.53

RADIOSENSITIVITY OF FANCONI ANEMIA FIBROBLASTS IN VITRO

Leskovac Andreja¹, Petrović Sandra¹, Joksić Ivana², Vujić Dragana³,Guć-Šćekić Marija³,

Slijepčević Predrag⁴and Joksić Gordana¹

¹ Institute of nuclear science „Vinča“;² Gynecology and Obstetrics Clinic „Narodni front“, Belgrade, Serbia;³Mother and Child Health Care Institute of Serbia “Dr. Vukan Čupić“, Belgrade;⁴Brunel University West London, Uxbridge, UK

Fanconi cells are highly sensitive to DNA crosslinking agents, but their response to ionizing radiation is still unclear. This aimed us to investigate in vitro cellular radiosensitivity of aplastic (AA) and Fanconi (FA) anemia fibroblasts employing enumeration of γH2AX foci and determination of apoptotic cells percentage. Foci of γH2AX, detected by immunofluorescence, are used for quantitative analysis of DSBs. The maximum of γH2AX foci in FA cells was 30 min, whereas in AA cells was 24 h after irradiation. In control cells apoptosis reached the maximum value 30 min and 24 h after irradiation. In both FA and AA cells maximum of cells dying via apoptosis were 2 and 24 h after irradiation. Results of the present study indicate disturbance of radioresponses of FA and AA cells seen as significant delay in apoptosis and cell cycle arrest. Furthermore, radioresponses 30 min after irradiation could be significant parameter for distinguishing between FA and AA cellular phenotypes. This study has shown that γH2AX foci represent very sensitive assay for estimation of intrinsic genome radiosensitivity, and demonstrate clear difference in behaviour of FA, AA and normal cells in response to ionizing radiation.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.54

COMPARATIVE ANALYSIS OF PREMATURE CENTROMERE DIVISION AND MICRONUCLEI IN PERSONS PROFESSIONALLY EXPOSED TO RADIONUCLIDES

Rakić Boban¹, Jovičić Dubravka², Vukov Tanja³, Pajić Jelena¹, Kovačević Radomir¹, Stevanović Milena⁴and Drakulić Danijela⁴

¹Institute of Occupational Medicine and Radiological Health “Dr Dragomir Karajović”, Belgrade;²The Faculty of Applied Ecology FUTURA, Singidunum university, Belgrade;

³Institute for biological research "Sinisa Stanković“, Belgrade, Serbia;⁴ IMGGI – Institute for molecula rbiology and genetic engeenering, Belgrade, Serbia

The aim of this paper was the comparative analysis of micronuclei (MN) and premature centromere division (PCD) in lymphocytes of peripheral blood in persons exposed to radionuclides. Two groups of patients were used for above mentioned analyses: 50 patients exposed to radionuclides (average age 45.24 ± 1.18, average exposition time 17.96 ± 1.15 years) and 30 control patients (average age 36.07 ± 1.11, average years of employment 10.77 ± 1.07 years). Our data showed that patients exposed to radionuclides had statistically significant higher frequencies of PCD and MN (Mann Whitney U Test, p<0.01). The sex and smoking have no influence on frequency of analyzed parameters. Also, there is significant positive correlation in frequencies of tPCD (PCD present in more then 10 chromosomes per metaphase) and MN. Using FISH method with probe pL1.84 repetitive DNA for chromosome 18 confirmed presence of PCD in interphase nuclei and metaphases. Taking into account that PCD was confirmed in persons with previously detected higher frequency of MN, our research suggests that PCD might be used as potential useful cytogenetic marker in persons professionally exposed to radionuclides.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.55

LOW DOSE GENOTOXICITY OF 4–bromo–N,N–diethyl–5,5–dimethyl–2,5–dihydro–1,2–oxaphosphol–2–amine 2–oxide IN MICE BONE MARROW CELLS

Kalcheva Petrova Vanya, Dragoeva Pencheva Asya, Kalchev Nikolov Karamfil and Enchev Dimitrov Dobromir

Faculty of Natural Sciences, University of Shumen, Shumen, Bulgaria

The chemistry of organophosphorus compounds is a subject of increasing interest, especially the heterocyclic compounds. There are data that some known organophosphorus compounds are mutagens. Although humans are chronically exposed to most environmental chemicals at low doses, genotoxicity assays with rodents are usually performed at high doses. The objective of this investigation was to determine genotoxicity of low doses (2.82x10^–6 μg/kg and 2.82x10^–9 μg/kg) of 4–bromo–N,N–diethyl–5,5–dimethyl–2,5–dihydro–1,2–oxaphosphol–2–amine 2–oxide (Br–oxph) on ICR mice bone marrow cells. Br–oxph induced chromosome aberrations in bone marrow cells after treatment for 3 h and during 24 h and 48 h recovery. The induction of chromosome aberrations demonstrates that the Br–oxph has a genotoxic effect at tested doses.

Acknowledgements: This work was supported by the Bulgarian Ministry of Education and Science, grant no. DO02-86/13.12.2008

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.56

LOW DOSE GENOTOXICITY OF 4–bromo–N,N–diethyl–5,5–dimethyl–2,5–dihydro–1,2–oxaphosphol–2–amine 2–oxide in Allium cepa L. ROOT TIP CELLS

Kalcheva Petrova Vanya, Dragoeva Pencheva Asya, Kalchev Nikolov Karamfil

and Enchev Dimitrov Dobromir

Faculty of Natural Sciences, University of Shumen, Shumen, Bulgaria

To provide a broad coverage of the mutagenic potential of a chemical, information on different experimental test-systems is required. In preliminary study we observed genotoxic effect of low doses of 4–bromo–N,N–diethyl–5,5–dimethyl–2,5–dihydro–1,2–oxaphosphol–2–amine 2–oxide (Br–oxph) in ICR mice bone marrow cells. The objective of the present study was to determine genotoxicity of Br–oxph at low doses (10^–12М and 10^–15М) using Allium cepa L. - test. Treatment with Br–oxph for 3 h produced alterations in the mitotic index and increased the frequency of chromosome abnormalities in Allium cepa root tip cells. These effects remained 48 h after the treatment. The data from the study showed the existence of cytotoxic and genotoxic effects of Br–oxph.

Acknowledgements: This work was supported by the Bulgarian Ministry of Education and Science, grant no. DO02-86/13.12.2008

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.57

ARE PET BOTTLES FOR MINERAL WATERS GENOTOXIC?

– TESTING WITH Allium Test

Stefanović Marina, Blagojević Jelena and Vujošević Mladen

Department for genetic research, Institute for biological research "Sinisa Stanković“, Belgrade, Serbia

Polyethylene terephthalate (PET) is relatively new material, which is widespread in practice for packaged food and mineral water. This material, characterized by thermostability, shatter resistance, lightweight, and transparency, has replaced PVC wrapping material. In the manufacture of PET, Sb₂O₃ is used as the catalyst and it was found that Sb can be realised in the bottled water. Potential genotoxic effects of two brand mineral waters from Serbia were analysed by Allium anphase-telophase test. After opening, the bottles with mineral waters were kept at three different temperatures, +4^oC, +50^oC and +60^oC, respectively. The results show that the warmed water samples significantly reduce onion growth and root length growth. Heating increases toxicity of mineral waters. On the other hand, the way the water is kept does not affect significantly the level of genotoxicity. This parameter is defined by frequency of aberrant cells, i.e. between 7.1% and 12.0%.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.58

EVALUATION OF GENOTOXIC EFFECT OF TOCOLYTIC RITODRINE, ANTIARRHYTHMIC VERAPAMIL AND ANTIBIOTIC ERYTHROMYCIN ON THE MICRONUCLEI FREQUENCY IN HUMAN PERIPHERAL BLOOD LYMPHOCYTES

Grujičić Darko¹,Milošević-Djordjević Olivera^1,2 and Stošić Ivana¹,

Arsenijević Slobodan² and Marinković Dragoslav³

¹Faculty of Natural Science, Kragujevac;²Faculty of Medicine, Kragujevac

³ Serbian academy of science, Belgrade

The aim of this study was to investigate the genotoxic effect of ritodrine, verapamil and erythromycin on human peripheral lymphocytes in vivo and in vitro, by application of cytokinesis block micronucleus (CBMN) test. In the sample of 32 patients, the average of MN frequency was significantly higher after combined therapy in comparison to the MN frequency in the same patients before therapy (p<0.001). In order to assess single effects of medicaments in the cultures of peripheral lymphocytes effects of different concentrations, for ritodrine 8,4x10^-6M-25,2x10^-4M, verapamil 0,56-11x10^-5M and erythromycin 0,68-5,45x10^-4M were tested. The increase of concentrations of ritodrine and verapamil increases the MN frequency. In the treatment with concentrations equivalent to daily therapeutical doses (DTD), both drugs single or combined induced statistically significant increase of MN (p<0.05; p<0.001). Erythromycin in tested concentrations did not change MN frequency (p>0.05). The obtained results show that ritodrine and verapamil in vivo and in vitro have significant genotoxic and co-mutagen effect.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.59

EVALUATION OF THE DNA DAMAGE LEVEL IN HUMAN LYMPHOCYTES EXPOSED TO EPHEDRINE IN VITRO

Đelić Ninoslav¹, Radaković Milena, Spremo-Potparević Biljana², Plećaš-Solarović Bosiljka²_,Živković Lada² and Bajić Vladan³

¹Department for biology, Faculty of veterinary medicine, University of Belgrade;²Institute for physiology, Faculty of pharmacy, University of Belgrade;³Institute for research and development, Department for biomedical research, Galenika, Belgrade, Serbia

Ephedrine is a sympathicomimetic isolated from the plant Ephedra vulgaris. It has similar chemical structure to adrenaline and pharmacodynamic action similar to catecholamines. Ephedrine acts mainly through release of catecholamines from their depots, but it also stimulates adrenergic receptors. Although ephedrine has not exhibited genotoxic effects in some test systems, to our knowledge it has not been tested in the Comet assay. Therefore, the objective of this study was to evaluate whether ephedrine can induce primary DNA damage in isolated human lymphocytes using in vitro Comet assay. Firstly, we examined a broad spectrum of ephedrine concentrations, in a range from 0.0005 μM to 500 μM. We found out that ephedrine does not cause significant departures from the negative control level (the solvent). Three concentrations (1, 50 and 300 μM) with acceptable cell viability (over 75%) were further tested with inhibitors of DNA repair (cytosine arabinoside and hydroxyurea) and we did not observe any significant changes compared with cultures without DNA repair inhibitors. It can be concluded that ephedrine does not exhibit genotoxic effects in the in vitro Comet assay on human lymphocytes.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.60

Genotoxic and citotoxic effects of fumagillin

Stanimirović Zoran and Stevanović Jevrosima

¹Department for biology, Faculty of veterinary medicine, University of Belgrade

Fumagillin, an antibiotic produced by Aspergillus fumigatus is used against microsporidiosis of bees and fish. In humans, fumagillin is suggested for the treatment of chronic microsporidial diarrhea and keratoconjunctivitis in patients with AIDS. Genotoxic effects of fumagillin were evaluated in vitro and in vivo. All tested concentrations of fumagillin (0.34, 0.68, 1.02, 3.07 and 9.20 μg/ml) applied to lymphocyte cultures increased the frequency of SCE significantly (p<0.001 or p<0.01). The three highest concentrations induced a significant (p<0.001) increase in structural CA, significant increase in MN formation and significant decrease in MI, PI, and NDI. In in vivo study BALB/c mice were treated with three doses of fumagillin (25, 50 and 75 mg/kg bw). All tested doses induced a significant (p<0.001) decrease in MI and a significant (p<0.001) increase in the frequency of MN. Significantly increased frequencies (p<0.01 or p<0.001) of numerical and structural CA were observed at 75 mg/kg bw. A significant (p<0.001) increase in the frequency of Rb(4.19) was observed at 50 and 75 mg/kg bw. These results confirm the genotoxic potential of fumagillin as well as its cytotoxic activity.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.61

GENOTOXICITY TESTING OF EFFECTS OF NATURAL AGENTS

Stanić Snežana¹, Matić Sanja¹ and Solujić Slavica²

¹Department of Biology, Faculty of Science, University of Kragujevac,

Kragujevac;²Department of Chemistry, Faculty of Science, University of Kragujevac,Kragujevac, Serbia

The genotoxicity effects of the acetone and watery extracts of plant Ambrosia artemisiifolia L. (Anacardiaceae) pollen and methanolic extract of plant Cotinus coggygria Scop. (Anacardiaceae) stem were investigated in vivo conditions using the sex-linked recessive lethal test (or SLRL test) on a eukaryotic model system Drosophila melanogaster.With applied experimental procedure we were able to quantify a mutegenic potential of agents of natural origin: two lactones, ambrosin and dihydroambrosin were identified in the acetone extract of Ambrosia artemisiifolia pollen, and pyrocatechol from the methanol extract of C. coggygria stem.The obtained results showed significant increases in the frequency of sex-linked recessive lethal mutations in premeiotic and postmeiotic germ cell lines of the tested males of species Drosophila melanogaster.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.62

EXAMINATION OF DIFFERENT RASPBERRY LEAVES (Rubus idaeus L.) METHANOL EXSTRACTS ON HELA CELLS VIABILITY AND PROLIFERATION

Vukelić Marija¹, Kitić Dušanka², Pavlović Dragana², Živanov-Čurlis Jelena¹,

Živković Jelena¹and Najman Stevo¹

¹Faculty of medicine, Institute for biomedical research, Niš

² Faculty of medicine, Integrated academic study of pharmacy, Niš, Serbia

The results of various phytochemical investigations show that raspberry leaves (Rubus idaeus L.) is rich source of flavonoids, ellagic acid and tannins. Experiments proved that the individual compounds belonging to these groups have antioxidant, antimicrobial, anti-inflammatory, antiviral and anticarcinogen activity. The aim of our study was examination of different raspberry leaves methanol exstracts on HeLa S3 cells viability and proliferation. Different methanol exstracts were prepaired by maceration in absolute methanol, ultrasount extraction in absolute methanol, maceration in 70% methanol and ultrasount extraction in 70% methanol. Efective exstracts concentrations were 0.0001mg/ml, 0.001mg/ml, 0.01mg/ml, 0.1mg/ml, 1mg/ml.Cell viability and proliferation was examined due to tetrazolium salt reduction ability (MTT test) and HeLa cells morphological characteristics after 24h and 72h incubation with extracts.The results of this investigation show that all investigated methanol extracts in these concentrations showed a mild impact on the viability of treated HeLa cells in comparison to the control group.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.63

ANTIGENOTOXIC PROPERTY OF AQUEOUS EXTRACT OF MEDICINAL MUSHROOM Agaricus blazei Murrill, ON THE GENOTOXICITY INDUCED BY MMS

Savić Tatjana¹, Patenković Aleksandra¹, Soković Marina¹, Glamočlija Jasmina¹, Radulović Višnja and Anđelković Marko^1,2

¹ Department for genetic research, Institute for biological research "Sinisa Stanković“, Belgrade, Serbia;² Faculty of Biology, University of Belgrade, Belgrade, Serbia

Agaricus blazei Murrill, popularly known as the „sun mushroom“, is a native mushroom in Brazil, that has been widely used in folk medicine mainly in the form of tea to treat various ailments (diabetes, hypercholesterolamia, osteoporosis, gastric ulcer, stress, etc.), but mostly for prevention and treatment of cancer.The wing Somatic Mutation And Recombination Test (SMART) in Drosophila melanogaster was used to study the antigenotoxic effect of mushroom Agaricus blazei Murrill, in combination with the alkylating agent methyl methanesulfonate (MMS). Two-day-old larvae, trans-heterozygous for recesive markers multiple wing hairs (mvh) and flare-3 [flr (3)], were fed for 24h with aqueous extract of Agaricus blazei. For antigenotoxicity studies separate 24h pre-treatment with aqueous extract of Agaricus blazei, followed by chronic treatment with a MMS, was done. Genetic changes induced in somatic cells of the wing imaginal discs lead to the formation of mutant clones on the wing blade. Our results showed that aqeous extract of Agaricus blazei can possess antigenotoxic and/or protective effect against the activity of mutagenic agent.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.64

ANTIMUTAGENIC POTENTIAL OF CAMPHOR, α/β THUJONE AND EUCALYPTOL IN THE BACTERIAL ASSAY SYSTEM

Nikolić Biljana, Milojević Nataša, Janković Ivana, Kolarević Stoimir, Jovanović Predrag, Jagodić Aleksandra, Vuković-Gačić Branka and Knežević-Vukčević Jelena

Department for microbiology, Faculty of Biology, University of Belgrade, Belgrade, Serbia

We have tested bioantimutagenic potential of monoterpenes Camphor (C), α/β Thujone (T) and Eucalyptol (E) and elucidated molecular mechanisms of mutagenesis using E. coli K12 assay system.The inhibition of UV- and 4NQO-induced mutagenesis was monitored by reversion test in repair proficient and NER deficient strains. All tested monoterpenes significantly reduced induced mutagenesis only in repair proficient strain, confirming the involvement of NER in bioantimutagenic effect. The effect of monoterpenes on the SOS induction kinetics following UV-irradiation was studied by measuring activity of b-galactosidase, expressed from sfiA::lacZ fussion. The effect on protein synthesis was determined by measuring activity of constitutive alkaline phosphatase. The growth rate, protein syntesis and SOS induction were differently affected by monoterpenes. T reduced growth rate, protein syntesis and the level of b-gal, with no effect on enzyme induction kinetics. E and C reduced the rate of b-gal induction, but C increased while E decreased the enzyme level, compared with the control. Obtained results indicate different mechanisms of increased efficiency of NER in the presence of monoterpenes.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.65

THE EFFECT OF RAT LIVER ENZYMES ON ANTIMUTAGENIC POTENTIAL OF MONOTERPENES AGAINST t-BOOH-INDUCED MUTAGENESIS IN E. coli

Mitić-Ćulafić Dragana, Janković Milena, Ćakić Sanja, Vuković-Gačić Branka and Knežević-Vukčević Jelena

Department for microbiology, Faculty of Biology, University of Belgrade, Belgrade, Serbia

Oxidative DNA damage is one of the most common threats to genome stability and may contribute to carcinogenesis, diabetes and ageing by inducing oxidative stress. In addition to cellular antioxidative defense mechanisms and DNA repair enzymes, some natural compounds can act as protective substances against reactive oxidative species (ROS) induced DNA damage. The aim of this work was to investigate the protective capacity of monoterpenes: Linalool (L), Eucalyptol (E) and Myrcene (M) against t-BOOH-induced oxidative mutagenesis in E. coli. The WP2 reversion assay was performed with strain IC202 oxyR, deficient in induction of antioxidative enzymes, with or without S9 fraction. The fraction contains rat liver cells enzymes involved in metabolic transformation and detoxification of xenobiotics.The obtained results showed that L, E and M possess high protective capacity against t-BOOH-induced mutagenesis. In the presence of S9 enzymes antimutagenic potential of tested monoterpenes was reduced, suggesting their processing to less protective metabolic forms. Obtained results are possibly relevant for the development of dietary or pharmaceutical cancer chemopreventive supplements.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.66

EVALUATION OF ANTIGENOTOXIC POTENTIAL OF LINALOOL, EUCALYPTOL AND MYRCENE AGAINST IQ- AND PhIP-INDUCED DNA DAMAGE

Mitić-Ćulafić Dragana¹, Žegura Bojana², Knežević-Vukčević Jelena¹ and Filipič Metka²

¹Department for microbiology, Faculty of Biology, University of Belgrade, Belgrade

²National Institute of Biology, Ljubljana, Slovenia

It has been reported that many plant components reduce the genotoxic effect of carcinogens, including heterocyclic amines. Monoterpenes: Linalool (L), Eucalyptol (E) and Myrcene (M) are strong antioxidants and free radical scavengers present in many medicinal and aromatic plants. In our previous work we have shown that L, E and M efficiently prevented t-BOOH- induced DNA damage and mutagenesis in bacteria and cultured human cells. In this work we studied antigenotoxic potential of L, E and M against food borne heterocyclic amines IQ and PhIP in human hepatoma HepG2 cells using Comet assay. The range of non-toxic concentrations of L, E and M was determined with MTT test. The cells were exposed to IQ or PhIP for 21h with or without L, E or M and subjected to the alkaline Comet procedure. The DNA damage induced by PhIP was significantly reduced (40-63%) by each of the tested substances, while inhibition of IQ-induced DNA damage was less efficient. Considering that PhIP is known to induce reactive oxygen species, we propose that more efficient protection against PhIP-induced genotoxicity is due to antioxidative activity of the tested substances.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.67

Antigenotoxic potential of tea infusion of Gentiana lutea in wing spot test OF Drosophila melanogaster

Patenković Aleksandra¹, Stamenković-Radak Marina^1,2, Nikolić Dragana, Marković Tamara and Anđelković Marko^1,2

¹ Institute for biological research "Sinisa Stanković, Belgrade

² Faculty of Biology, University of Belgrade, Belgrade

The root of Gentiana lutea is an ingredient of many proprietary medicines, which has a long history of use as an herbal bitter in the treatment of digestive and numerous other disorders. Gentian root possesses therapeutic effects due to the presence of bitter glycosides, with main components gentiopicrin, gentiamarin and swertiamarin.

The antigenotoxic action of gentian was evaluated by the wing Somatic Mutation and Recombination Test (SMART) on D. melanogaster. This test provides sensitive, rapid and reliable verification of mutational and recombinational potential of various chemicals and their antigenotoxic property. Two mutagens, methyl methanesulfonate as an alkylating agent and hydrogen peroxide as an oxidative reagent, were used to evaluate the antigenotoxicity of gentian. Third-instar larvae, trans-heterozygous for two genetic markers, multiple wing hairs (mwh) and flare (flr), were fed mutagens alone and in combination with tea infusion of gentian. Results reveal the difference in antimutagenic effects of gentian infusion. Usage of mutagens with different mechanisms of affecting DNA molecule and different treatments explain mechanisms of antimutagenic effect of G. lutea.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.70

HEDGEHOG SIGNALING PATHWAY IN DEVELOPMENT AND TUMOR FORMATION

Milenković Ljiljana

Stanford University Medical Center, Stanford, USA

Hedgehog (Hh) signaling pathway, first identified in a genetic screen for genes that control embryonic development in the fruit fly, regulates cell proliferation and differentiation in many tissues and organs, not only in flies, but also in vertebrates, including humans. Mutations in the genes components of the pathway can lead to birth defects and cancer. Inappropriate activation of the pathway has been implicated in several types of cancer, including medulloblastoma, basal cell carcinoma, rhabdomyosarcoma and others. That is one of the reasons we would like to fully understand how this signaling pathway works and find ways to modify it, in cases when it doesn't function properly. An interesting insight into Hh signaling in vertebrates came several years ago from genetic evidence in mice that suggests that the primary cilium, a cell organelle present in most cells in vertebrates, has an important role in processing of the Hh signal. We are using genetic, cell biological and biochemical approaches to further understand the role of cilia as a center for receiving and transmitting the Hh signal. Recent advances towards this goal will be presented.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.72

POLYMORPHISM C677T IN THE GENE ENCODING FOR METHYLENETETRAHYDROFOLATE REDUCTASE

IN DIFFERENT VASCULAR DISORDERS

Novaković Ivana¹, Damnjanović T.¹, Sango Violeta², Mladenović O.¹, Maksimović N.¹, Mirković Duško³, Antonijević Nebojša⁴, Simić-Ogrizović S.⁵, Sternic N.⁶ and Luković Ljiljana¹

¹Institute of Human Genetics, School of Medicine, Belgrade, ²Laboratory of Medical Genetics, HC Jagodina, Jagodina, ³Institute of Medical Biochemistry, ⁴Institute of Cardiovascular Diseases, ⁵Institute of Nephrology and Urology, ⁶Institute of Neurology, CCS, Belgrade, Serbia

It is well documented that higher plasma level of the amino acid homocysteine has atherogenic and prothrombotic action. For these reasons homocysteine metabolism is studied in large number of phisiological and pathological conditions. Activity of the enzyme methylene-tetrahydrofolate reductase (MTHFR) is one of the key points of homocysteine metabolism, involved in the remethylation of homocysteine to methionine. Gene polymorphism MTHFR C677T reduced the enzyme activity and is associated with risk of the hyperhomocystenemia. During the last five years we analyzed MTHFR C677T polymorphism and ists association with vascular disorders in different groups. In whole population established frequency of 677T allele and TT genotype was about 0.40 and about 15%, respectively, but data varied with age. Our studies showed association of T allele and TT genotype with higher lipid level and higher artherial tension in healthy school-aged child, as well as in young patients with stroke and in hemodyalisis patients. On the contrary, no significant association observed for 677 T allele and TT genotype in young patients with myocardial infarction and with pulmonary embolism.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.74

CYTOGENETICS-THE PRESENT, THE FUTURE AND PERSPECTIVE

Milićević Radomir, Branković Ljiljana, Radulović D., Jugović D., Stanković T., Stamenković H., and Kamenov B.

Clinical Center Nis, Clinical for child internal diseses, Laboratory for Immunology and Genetics, Nis, Serbia

Chromosome aberration frequency in the general population is approximately 0,6% though they occur more often ih high risk groups. The risk group mainly consists of pregnant women above 35 years, young pregnant women who have underwent alternative diagnostic methods wich raised doubts about their condition, couples with primary sterility etc.We present in this study the results of our laboratory for the year 2008. We analysed karyotype of pheripheral blood, bone merrow and amniotic cells after cultivation in RPMI or Quantum medium. All specimen were G-banded using trypsin- Giemsa stain. A total of 961 karyotypes has been analyzed: 311 karyotypes obtained from blood counted 33 abnormal cases, 481 from amniotic had 11 aberrant cases and 179 karyotypes obtained from bone marrow had 37 aberrant metaphases.Cytogenetic is a method with significant and irreplaceble role in the discovery of chromosomal aberrations. Detection of chromosomal abnormalities provides the opportunity to plan further treatment of reproduction disorders.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.75

TYPES OF bcr-abl REARRANGEMENTS IN SERBIAN POPULATION AND A LEVEL OF EXPRESSION IN CHRONIC MYELOID LEUKEMIA PATIENTS

Todorić-Živanović Biljana^a, Strnad Milica^a, Stamatović Dragana^a, Grujović Jelena^b,

Tanić Nikola^c, Ilić Vesna^aand Magić Zvonko^a

Military Medical Academy^a, Applied biosystems^b, Institute for Biological research^c, Belgrade, Serbia

Chronic myeloid leukemia (CML) is a clonal myeloproliferative disorder of a pluripotent chematopoetic stem cell. In more than 95% of cases, it is characterized by the presence of chimeric bcr-abl gene which is formed by the translocation t(9;22)(q34;q11) or by other molecular mechanisms. Although well known by it’s clinical features it is molecularly heterogenic disease. The Bcr-abl, p210kD, protein with its elevated tyrosine kinase activity has a central role in pathogenesis of CML. Those knowledge gave rise for developing molecularly targeted therapy for CML with tyrosine kinase inhibitors. In this study we investigated types of bcr-abl rearrangements in CML patients (pts) in Serbian population. We analyzed 112 patients by RT-PCR method. Level of bcr-abl expression was quantified by the absolute real-time PCR (RQ-PCR) method in 20 treated pts. The level of expression in 15 de novo, untreated, pts was measured too, for constructing base line. In 85 (76.9%) pts, b3a2 form of bcr-abl gene was detected and b2a2 form in 26 (23.2%). One pt (0.9%) was bcr-abl negative, bath with e1a2 form of bcr-abl in blastic transformation of the disease. The level of expression of treated pts was compared with base line.Predomination of b3a2 form of bcr-abl gene, in our population, is in correlation with literature data for European population.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.76

MOLECULAR DIAGNOSTIC OF CHILDHOOD ALL

Lakić Nina¹, Krstić Aleksandra¹, Guć-Šćekić Marija¹, Kuzmanović Miloš², Mićić Dragan², Janjić Dragana³, Konstantinidis Nada⁴and Kostić Gordana⁵

¹Laboratory for Medical Genetics, Mother and Child Healthcare Institute, Belgrade; ²Department for Hematological Oncology, Mother and Child Healthcare Institute, Belgrade; ³Department for Hematological Oncology University Children's Hospital in Belgrade;⁴Center for Hematological Oncology Institute for health care of Children and Youth of Vojvodina, Novi Sad;⁵Department for Hematological OncologyChildren hospital, Niš

According to the BFM protocol for treatment of childhood ALL, molecular genetic analyses are a part of routine diagnostic procedure. They play an important role in risk group classification, choice of therapy and detection of MRD during follow-up. After several large studies it had been shown that the presence of fusion genes BCR/ABL, TEL/AML1, MLL/AF4 i PBX1/E2A in transformed blood cells are specific for ALL in children. Developing of RT-PCR technique allow us the detection of fusion gene transcripts of these four the most frequent rearrangements in ALL with high target sensitivity of 10 ^-6cells.RT-PCR analyses for detection of the genetic rearrangements mentioned above have been performing at the Laboratory of Medical Genetics, Mother and Child Health Care Institute, Serbia according to standard BIOMED protocol since May 2007.A total of 87 patients with ALL were analysed using RT-PCR method, during the diagnosing procedure. The BCR/ABL fusion gene (p190 type) was detected in four children (≈5%), equal to the presence of PBX1/E2A rearangement (≈5%). Hibride gene MLL/AF4 was seen in two patients (≈2%) and the most frequent was TEL/AML1 rearangement detected in 19 (≈22%) patients.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.77

EKSPRESSION OF BCL2 PROTEIN AND AMPLIFICATION OF C-MYC GENE IN PATIENTS WITH CHRONIC MYELOID LEUKEMIA

Strnad Milica, Brajušković Goran, Strelić Nataša, Todorić-Živanović Biljana, Stamatović Dragana and Magić Zvonko

Military Medical Academy, Belgrade, Serbia

Chronic myeloid leukemia (CML) represents the malignant myeloproliferative disease developed out of pluripotent hematopoietic stem cell that contains fusion bcr-abl gene. Disorders that occur in the process of apoptosis represent one of the possible molecular mechanisms that bring about disease progress. Within our study we carried out the analysis of the presence the amplification of c-myc oncogene as well as the analysis of the changes in expression of Bcl-2 in patients with CML. Our study included 25 patients with CML (18 in chronic phase, 7 in blast transformation). Using immunohistochemical APAAP method we analyzed the expression of cell death protein in mononuclear bone marrow cells of 25 CML patients. By differential PCR method we followed the presence of amplified c-myc gene in mononuclear peripheral blood cells. The level of expression of Bcl-2 protein is considerably higher in bone marrow samples of patients undergoing blast transformation of disease. The amplification of c-myc gene has been detected in 30% patients in blast transformation of disease. The results of this work have shown that expression of Bcl-2 protein and amplification of c-myc gene are in correlation with disease progression.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.78

RESULTS OF CYTOGENETIC AND MOLECULAR TESTING IN SERBIAN PATIENTS WITH NIJMEGEN BREAKAGE SYNDROME

Ćirković Sanja¹, Guć-Šćekić Marija¹, Pašić Srđan², Radivojević Danijela¹and Jovanović Ankica³

¹Laboratory for Medical Genetics, Mother and Child Healthcare Institute „Dr Vukan Čupić”, Belgrade;²Department for Imunology, Mother and Child Healthcare Institute „Dr Vukan Čupić”, Belgrade, ³Department for Hematological Oncology, Mother and Child Healthcare Institute „Dr Vukan Čupić”, Belgrade, Serbia

Nijmegen breakage syndrome (NBS) is a rare autosomal recessive disorder, characterized by microcephaly, facial dysmorphism, immunodeficiency and cancer susceptibility, with increased cellular hypersensitivity to specific mutagenic agents such as bleomycin(BLE). Molecular diagnosis of NBS is based on screening for the presence of mutations in NBN gene, especially 5-bp deletion(657del5) in exon 6, the most frequent mutation in Slavic population.We investigated five patients with NBS, diagnosed and treated at the Mother and Child Health Care Institute of Serbia (2005-2007). Cytogenetic testing was carried out on peripheral blood cultures using standard procedure.Only three of four blood cultures were successful and exhibited chromosome breakage BLE-induced increases (0.27-0.81breaks/cell) and aberrations including chromosomes 7 and 14.Molecular analysis for the presence of 657del5 mutation in NBN gene was carried on DNA samples from peripheral blood, using modified PCR method on PAGE gel.Homozygosity has confirmed for all (five) tested patients. Early and precise diagnosis of NBS is very important for the management of the disease and for genetic counseling of the families at risk.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.79

PROGNOSTIC SIGNIFICANCE OF TUMOR SUPPRESSOR GENES HYPERMETHYLATION IN ORAL SQUAMOUS CELL CARCINOMA

Šupić Gordana¹, Kozomara Ružica², Branković-Magić Mirjana³, Jović Nebojša²

and Magić Zvonko ¹

¹Institute for Medical Research, Military Medical Academy, Belgrade, ²Clinic for Maxilofacial Surgery, Military Medical Academy, Belgrade, ³Institute for Oncology and Radiology, Belgrade, Serbia

Oral squamous cell carcinoma (OSCC) is characterized by high mortality rate and rising incidence in developed countries. Epigenetic modifications are changes in gene expression that are not coded in the DNA sequence. DNA methylation of CpG islands in promoters causes transcriptional silencing of genes and malignant transformation. The genes selected for our investigation are involved in cellular processes that are crucial for malignant transformation including cell cycle control (p16), apoptosis (DAPK), Wnt signaling (APC), cell-cell adhesion (E-cadherin), and DNA repair (MGMT, WRN). In 77 patients with OSCC, methylation was determined by methylation-specific PCR. Hypermethylation of p16 gene promoter was detected in 58.4% of samples, MGMT in 33.76%, DAPK in 36.36%, WRN in 23.37%, E-cadherin in 42.86%, and APC in 18.18% of OSCC samples. Patients with E-cadherin promoter methylation had significantly worse overall and cancer-specific survival (χ², p<0.05), while hypermethylation of other genes did not have prognostic significance. E-cadherin promoter hypermethylation could be molecular marker for the poor survival in OSCC.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.80

PATTERN OF TRISOMY 1Q IN HEMATOLOGICAL MALIGNANCIES: A SINGLE INSTITUTION EXPERIENCE

Đorđević Vesna^a, Denčić-Fekete Marija ^a, Jovanović Jelica^a, Drakulić Danijela^b, Stevanović Milena^b, Janković Gradimir^a, Gotić Mirjana^a

^aInstitute of Hematology, Clinical Center of Serbia, Belgrade, Serbia

^bInstitute of Molecular Genetics and Genetic Engineering, Belgrade, Serbia

An extra copy of 1q usually originates from the translocated unbalanced derivative chromosome, isochromosome, or ‘‘jumping translocation.’’ We report a pattern of partial trisomies and unbalanced whole-arm translocations of 1q in 10 patients: 5 with myelodysplastic syndrome, 3 with acute myeloid leukemia, and a single patient with acute lymphoblastic leukemia and myeloproliferative syndrome. The trisomy of 1q was registered as the sole karyotype aberration in one patient, while it was accompanied by a limited number of additional chromosomal changes in nine patients. Analyzed patients are a subset of a larger group of 92 adults carrying a wide variety of chromosome 1 anomalies within a complex cytogenetic context observed over a period between 1994 and 2006 in a panel of 3786 hematologic patients at the Institute of Hematology in Belgrade. Chromosomes were prepared according to standard procedures and HG-banding. Conventional cytogenetics was supplemented by fluorescence in situ hybridization (FISH) with a probe specific for the paracentric region of 1q. Whole-arm 1q translocations involved chromosomes: Y, 7, 14, 15, 16, 16 and 19. This study suggests that extra copy of 1q as the sole cytogenetic abnormality may be sufficiently mutagenic to favor leukemogenesis and hematopoietic tissue degeneration (trilineage myelodysplasia).

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.81

GENETICS IN PHARMACOLOGY: AN INDIVIDUALIZED MEDICINE

Jelenković Ankica

Institute for biological research "Sinisa Stanković“, Belgrade, Serbia

Prescribing drugs is a serious responsibility. The effects of medication varies from person to person, which can be, beside other reasons, due to the fact that human genetic profiles are not identical. The first such observations appeared more than 50 years ago. That was the muscle relaxant suxamethonium chloride that prolonged apnea in some people, as well as the drugs metabolized by N-acetyltransferase with very different blood concentrations of isoniazid and procainamid. It is known that genetic factors influence drugs' pharnmacokinetic and pharmacodinamic. The term pharmacogenetcs is used since late 1950s. It is generally regarded as the study or clinical testing of genetic variation that gives rise to differing response to drugs. Phenomenon of individual variations to drugs progressed toward pharmacogenetic science which promise to incorporate into clinical practice a concept of „tailor-made medicine“, individualzed medicine, in which drugs and drug combinations become coordinated with each individual's unique genetic profile. The wider use of pharmacogenetic testing is viewed by many as an outstanding opportunity to optimize prescribing safety, efficacy and cost.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.84

FAMILY HISTORY OF GRAVES-BASEDOW DISEASE AND ENVIRONMENTAL RISK FACTORS IN ETIOLOGY OF THIS DISEASE

Živanović Dušica¹, Šipetić Sandra²,Stamenković-Radak Marina³ and Milašin Jelena⁴

¹Dept of Gynecology and Obstetics, Health Center Ćuprija, Ćuprija;²Institute of Epidemiology, Medical School, University of Belgrade;³Faculty of Biology, University of Belgrade;⁴Institute of Biology and Genetics, School of Dentisty, University of Belgrade

Graves-Basedow disease (GBD) is a common multifactorial syndrome, determined by several genes and environmental factors. Our study aimed at unveiling risk factors for developing GBD and to compare risk factors in affected individuals with positive family history (PFH) and individuals without PFH of GBD. This cross-sectional study was conducted in Ćuprija and icluded 262 individuals (132 with GBD and 130 healthy subjects). Data were collected on basic demographic characteristics, exposure to various chemical and physical agents, stress, smoking and PFH of GBD. Affected individuals were older (above 50) (p=0,020), exposed to stress (p=0,024) to physical agents (p=0,031), and had significanly (p=0,000) more relatives with GBD than the control. Among the affected individuals with PFH of GBD, the number of women was significantly higher (p=0,000), than in the affected individuals without PFH. Gender, age, PFH and exposure to physical agents were identified as potential risk factors for the increased incidence of GBD. Different risk factors are probably responsible for Graves-Basedow disease among the affected individuals with and without PFH.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.85

POPULATION GENETICS IN PRECLINICAL PRACTICE: PRESENTATION OF 18 YEARS WORK CARRIED AUT AT GENETIC COUNSELLING OFFIS OF THE MEDICAL PRACTICE IN JAGODINA

Janakova Zorica

Healthcare Center, Jagodina

The work shows principles successfully organized prevention of chronic comman noncontagious diseases and basic levels of activity carried out at this family counselling offis. From the aspect of populational genetics and preclinical practice and using the examples of Diabetes mellitus, we establish the genetical, constitutional, physiological and environmental parameters of the most effective selection of the persons (families) in order to diagnose liability .The work shows Population and Genetic Questionnaire of pregnant and child-bearing woman in the systematic procedure of screening, long-term monitoring and control genetic, physiological and environment health of the population.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.86

CASE STUDY OF POSSIBLE FAMILIAL ANTIPHOSPHOLIPID SYNDROME

Popović-Kuzmanović Dragana¹, Novaković Ivana², Stojanović Ljudmila¹, Miković Danijela³, Mirković Duško⁴, Luković Ljiljana²and Aksentijević Ivona⁵

¹Clinical center ”Bežanijska Kosa”,²³Institute of Biology and Human genetics, School of Medicine, University of Belgrade,³Transfusion Institute of Serbia, ⁴Institute of Biochemistry, KCS, Belgrade, Serbia,⁵National Institutes of Health, Arthritis and Rheumatism Branch, Bethesda, USA

Antiphospholipid syndrome (APS) may have a familial association. This familial tendency could be genetically determined. In this study we analyzed family consisted of 7 members, with three affected members: father and two suns. The diagnosis was established by clinical signs, imaging methods, presence of anti cardiolipin and anti β₂ glicoprotein I antibodies, homocysteine levels, coagulation factors as well as protein C, protein S, antithrombin III deficiencies and APC resistance. DNA polymorphisms in genes encoding for factor V (G1691A), prothrombin (G20210A), MTHFR (C677T and A1298C) were analyzed by PCR/RFLPS method. Plasma Hcy was measured by HPLC method. Father and two suns had pulmonary embolism. Nobody have positive ANA, dsDNA and RF. The values of anti cardiolipin anti β₂ glycoprotein I antibodies were border line positive. Hcy levels and platelet number were within normal range. All members of family had low value of factor V, and father and both of suns also had low factor II, VII and X. Father and his suns had low protein C and S, but they are on anticoagulant therapy. The results of genetic studies will be discussed in the context of biochemical and clinical phenotypes. Further investigations will include analyses of new DNA polymorphisms, which could be associated with family disease.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.87

PHARMACOGENETICS, DRUG INTERACTIONS AND ADVERSE DRUG REACTIONS

Jelenković Ankica

Institute for Biological Research "Sinisa Stanković“, Belgrade, Serbia

Over half a century ago it has been recognized that genetic determined variations between people contribute to interindividual differences in the response to the same dose of the same drug, given separately or together with other drugs. Pharmacogenetics is a growing field of research and medicine focuses on the interaction between a single gene variation and drug therapy. Genetic polymorphism are involved in drug efficacy, drug-drug interactions and adverse drug reactions (ADR) variability, because most of drug effects are defined by the interplay of multiple gene products through the entire drug pathway in the human body, such as metabolizing enzymes, transporters, receptors and/or ion channels. Millions of people worldwide are affected per annum by ADR, which are among leading causes of death. Pharmacotherapy improvement, especially that of medications with narrow therapeutic indices, is expected to be obtain with identification of gene variants encoding key components of drug targets (i.e. receptors) and all aspects of drug disposition (i.e. absorption, distribution, metabolism and excretion) responsible for increased risk of adverse drug reactions and unwanted drug interaction.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.88

CANDIDATE GENE POLYMORPHISMS AND SUSCEPTIBILITY TO ABDOMINAL AORTIC ANEURYSM

Cvetković Dragana¹, Cvetković Slobodan², and Novaković Ivana³

¹Faculty ofBiology, University of Belgrade, Belgrade

²Clinic for Vascular Surgery, Institute of Cardiovascular diseases Clinical Center of Serbia

³Institute of Human Genetics, School of Medicine, University of Belgrade

Abdominal aortic aneurysm (AAA) is a common vascular disease with high fatality rate. Genetic polymorphisms potentially related to susceptibility to aneurysm formation and disease outcome have attracted much interest. The aim of this study was to examine the association between AAA and following candidate gene polymorphisms: -1562 (C→T) of matrix metalloproteinase (MMP) 9 gene, common e2/e3/e4 polymorphism of apolipoprotein E (APOE) gene, I/D of angiotensin converting enzyme gene (ACE), 677 (C→T) of methylenetetrahydrofolate reductase (MTHFR) gene and -819 (C→T) of interleukin (IL) 10 gene. The study included patients who underwent surgery for AAA during 2005-2006 at the Institute for Cardiovascular Diseases CCS and healthy controles comparable for sex and age. Significant association with AAA was found only for ACE I/D polymorphism (OR=2.897, 95% CI 1.409-5.955, p=0.003). None of the other polymorphisms was significantly related with susceptibility to AAA. Though it is not likely that a single gene polymorphism has a key role in aneurysm formation, candidate gene approach provides better understanding of pathogenesis and individual susceptibility to AAA and may provide potential therapeutic targets.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.89

THE ASSOCIATION OF IL-6 -174G/C GENE POLYMORPHISM WITH CAROTID ATHEROSCLEROSIS IN POPULATION OF SERBIA

Stojković Ljiljana¹, Živković Maja¹, Radak Đorđe², Kolaković Ana¹, Stančić Olja¹, Stanković Aleksandra¹ and Alavantić Dragan¹

¹Institute of Nuclear Sciences VINČA, Laboratory for Radiobiology and Molecular Genetics, Belgrade, Serbia

²Dedinje Cardiovascular Institute, Vascular Surgery Clinic, Belgrade, Serbia

If chronic inflammation plays a causal role in atherogenesis, individuals with proinflammatory gene variants would be expected to develop atherosclerosis. The IL-6 gene were chosen because is an important component of the upstream inflammatory process and the -174G/C polymorphisms already implicated in cardiovascular disease. The aim of was to associate the IL-6 -174G/C polymorphism with development of carotid plaques. Materials and methods: Genomic DNA was isolated from blood cells of 278 controls and 136 patients with carotid atherosclerosis. The genotypes were determined by tetra-primer ARMS PCR and visualized by agarose gel electrophoresis. Results: The genotype distribution wasn’t significantly different in patients (GG 39.71%, GC 44.12%, CC 16.18%) compared to controls (GG 41.73%, GC 46.40%, CC 11.87%). Conclusion: The IL-6 gene polymorphism is not associated with advanced atherosclerotic phenotype such as plaque presence, in population of Serbia. As IL-6 is expressed in human atherosclerotic plaques there is the possibility that the level of its expression plays the more important role in this clinical phenotype than genotype of investigated polymorphism.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.90

MATRIX METALOPROTEINASE-9 GENE POLYMORPHISM -1562C/T AS SUSCEPTIBILITY FACTOR FOR CAROTID ATHEROSCLEROTIC PLAQUE DEVELOPMENT

Kolaković Ana¹, Stojković Ljiljana¹, Radak Đorđe², Đurić Tamara¹, Radak Sandra², Stanković Aleksandra¹, Alavantić Dragan¹and Živković Maja¹

¹Institute of Nuclear Sciences VINČA, Laboratory for Radiobiology and Molecular Genetics, Belgrade, Serbia;²Dedinje Cardiovascular Institute, Vascular Surgery Clinic, Belgrade, Serbia

The matrix metalloproteinase 9 (MMP-9) has been shown to play role in VSMC migration and proliferation by degradation of the basement membrane. MMP-9 expression is increased after vascular injury. The MMP-9 -1562 C/T gene polymorphism results in higher promoter activity of the T allele. The higher prevalence of –1562T allele was associated with coronary artery stenosis. The aim was to investigate the association of the MMP-9 -1562C/T polymorphism and susceptibility for development of carotid plaques. Genomic DNA was isolated from blood cells of 278 control subjects and 136 patients with carotid atherosclerosis. The genotypes were determined by tetra-primer ARMS PCR method previously developed by us and visualized by MADGE. We did not find the significant difference in genotype frequencies between controls: CC 70.82%, CT 27.05%, TT 2.14%, and patients: CC 74.26%, CT 25.74%, TT 0%. The allele frequencies didn’t differ as well (controls: C 0.84, T 0.16, patients: 0.87, 0.13. MMP-9 gene polymorphism -1562C/T is not the susceptibility factor for development of carotid atherosclerotic plaques in population of Serbia.

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Book of Abstracts IV CONGRESS OF THE SERBIAN GENETIC SOCIETY

Tara, Serbia, June 01-05, 2009.

pp.91