GENETIKA, Vol. 53, No. 1 (2021)
Motonori TOMITA, Keiko NAKATSUKA, Natsuko MORITA, Evans LAGUDAH, Rudi APPELS
NBS-LRR-CONTAINING CLASS OF SALICYLIC ACID-INDUCED GENE TRANSCRIPT IN RYE [Abstract] [Full text]
Hasan KOÇ
GENOTYPE-BY-ENVIRONMENT INTERACTION FOR SEED YIELD AND OIL CONTENT OF SAFFLOWER (Carthamus tinctorius L.) GENOTYPES [Abstract] [Full text]
Tejpal Singh SRAN, S.K. JINDAL and Neena CHAWLA
GENOTYPE BY ENVIRONMENT INTERACTION FOR QUALITY TRAITS IN CHILLI PEPPER (Capsicum annuum L.) [Abstract] [Full text]
Rahime CENGİZ, Mesut ESMERAY
DEVELOPMENT OF LATE TEMPERATE IN VIVO HAPLOID
INDUCERS [Abstract]
[Full
text]
Farinaz ZIAEE, Mohammadreza HAJJARI, Seyed Reza KAZEMINEZHAD, Mehrdad BEHMANESH
SNHG7 HAS AN ONCOGENIC ROLE IN COLORECTAL CANCER VIA POTENTIAL SPONGING OF MIR-485-5P AND MIR-193A-5P; IN SILICO APPROACH [Abstract] [Full text]
Seyed Arsalan ASADI, Alireza POURMOHAMMAD, Ali-Asghar ALILOO, Khoshnood ALIZADEH, Mohammad Bagher HASSANPOURAGHDAM
FORAGE YIELD COMPONENTS OF SOME TOLERANT AND SENSITIVE GRASS PEA GENOTYPE SEEDLINGS AFFECTED BY THE SALINITY STRESS [Abstract] [Full text]
Muhammed KUPE, Sezai ERCISLI, Tatjana JOVANOVIC –
CVETKOVIC, Sadiye Peral EYDURAN, Rayda Ben AYED
MOLECULAR CHARACTERIZATION OF WILD GRAPES FROM
NORTHEASTERN PART OF TURKEY [Abstract] [Full
text]
GENETIC DIVERSITY AND SPATIAL STRUCTURE OF THE IMPERILED EUROPEAN POPULATION OF Malus trilobata IN GREECE [Abstract] [Full text]
Mohammad Reza NAROUI RAD and Gholamali KEIKHA
FIRST EVALUATION OF MELON (Cucumis melo. L) LANDRACES UNDER HIGH TUNNEL CONDITION IN SISTAN [Abstract] [Full text]
Seda ORENAY-BOYACIOGLU, Olcay BOYACIOGLU
CHEMOSENSITIZING EFFECTS OF METHYL JASMONATE ON PACLITAXEL-RESISTANT ANDROGEN-DEPENDENT AND INDEPENDENT PROSTATE CANCER CELL LINES [Abstract] [Full text]
Ruomei WANG, Junwei ZHANG , Fei LUO, Nannan LIU, Slaven PRODANOVIC, Yueming YAN
CLONING AND MOLECULAR CHARACTERIZATION OF TWO NOVEL LMW-m TYPE GLUTENIN GENES FROM Triticum spelta L. [Abstract] [Full text]
Velimir RADIĆ, Igor BALALIĆ, Miloš KRSTIĆ, Ana MARJANOVIĆ-JEROMELA
CORRELATION AND PATH ANALYSIS OF YIELD AND YIELD COMPONENTS IN WINTER RAPESEED [Abstract] [Full text]
Esra CEBECI, Huseyin PADEM, Ali Fuat GOKCE
MORPHOLOGIC AND CYTOPLASMIC ASSESSMENTS OF BULB ONION (Allium cepa L.) LANDRACES [Abstract] [Full text]
Verica TAKAČ, Ankica KONDIĆ-ŠPIKA, Dragana TRKULJA, Ljiljana BRBAKLIĆ, Vesna ŽUPUNSKI, Vladimir AĆIN, Sanja MIKIĆ
PHENOTYPIC AND MOLECULAR DIVERSITY OF WHEAT SPECIES (Triticum spp.) IN RELATION TO PLANT HEIGHT AND HEADING TIME [Abstract] [Full text] [Supplement]
Himani SHARMA, Parul SHARMA and Rajnish SHARMA
TRANSFERABILITY OF APPLE AND PEAR SSRS TO OTHER TEMPERATE POME FRUIT CROPS OF FAMILY ROSACEAE [Abstract] [Full text]
Vladimir Ivanovich TRUKHACHEV, Marina Ivanovna SELIONOVA, Irina Ivanovna DMITRIK, Milan P. PETROVIC, Violeta Caro PETROVIC, Dragana RUZIC-MUSLIC, Nevena MAKSIMOVIC
GENETICAL INFLUENCE ON HISTOLOGICAL STRUCTURE AND
CHEMICAL COMPOSITION OF
MUSCULAR TISSUE IN SHEEP [Abstract] [Full
text]
Stefan M. MARKOVIĆ, Desimir
KNEŽEVIĆ, Nenad M. NEŠOVIĆ, Nevena H. DJUKIĆ
HEAT-INDUCED
ACCUMULATION OF PROLINE AND YIELD COMPONENTS IN GENETICALLY DIVERGENT CEREAL VARIETIES
[Abstract]
[Full
text]
Mauricio PEÑUELA, Angie PATIÑO-MONTOYA, Andrés PEÑA-CRUZ, Heiber CÁRDENAS
POPULATION GENETICS IN MULTIPLE SCALES: GENETIC MICROSTRUCTURE OF A CAT POPULATION IN COLOMBIA [Abstract] [Full text]
Renata ILIČIĆ, Tatjana POPOVIĆ, Sanja MARKOVIĆ, Aleksandra JELUŠIĆ, Ferenc BAGI, Slobodan VLAJIĆ, Slaviša STANKOVIĆ
GENETIC DIVERSITY OF Pseudomonas syringae PV. syringae ISOLATED FROM SWEET CHERRY IN SOUTHERN AND NORTHERN REGIONS IN SERBIA [Abstract] [Full text]
THE INFLUENCE OF GENETIC β -LACTOGLOBULIN POLYMORPHISM ON THE QUANTITY AND QUALITY OF MILK OF THE SIMMENTAL BREED IN SERBIA [Abstract] [Full text]
M. WHETO, O.O, ISMAILA, M.A. ADELEKE, A.S. ADENAIKE, S.O. PETERS, A. YAKUBU, A.O. ADEBAMBO, C.O.N. Ikeobi and O.A. ADEBAMBO
SEQUENCE ANALYSES OF INSULIN-LIKE GROWTH FACTOR 1 GENE IN NIGERIAN INDIGENOUS AND ARBOR ACRE CHICKENS [Abstract] [Full text]
Tatjana VUJOVIĆ, Darko JEVREMOVIĆ, Tatjana MARJANOVIĆ, Đurđina RUŽIĆ
CRYOPRESERVATION OF SERBIAN AUTOCHTHONOUS PLUM ‘CRVENA RANKA’ USING ALUMINIUM CRYO-PLATES [Abstract] [Full text]
IN VITRO PROPAGATION OF BLACK CUMIN (Nıgella satıva L.) PLANTS [Abstract] [Full text]
Sanida ARSLANOVIĆ-LUKAČ, Nenad ĐURIĆ, Veselinka
ZEČEVIĆ, Jasmina BALIJAGIĆ, Dobrivoj POŠTIĆ
THE EFFECT OF YEAR AND GENOTYPE ON PRODUCTIVITY AND QUALITY OF POTATO [Abstract] [Full text]
Vesna PERIĆ, Mirjana SREBRIĆ, Ana NIKOLIĆ, Danijela RISTIĆ, Snežana MLADENOVIĆ DRINIĆ, Zoran DUMANOVIĆ
STABILITY OF YIELD AND SEED COMPOSITION IN EARLY MATURING SOYBEAN GENOTYPES ASSESSED BY AMMI ANALYSIS [Abstract] [Full text]
Boban ANICIC, Nadja NIKOLIC, Jelena CARKIC, Drago JELOVAC, Zoran JEZDIC, Branko DOZIC, Vesna DANILOVIC, Jelena MILASIN
MATRIX METALLOPROTEINASE 7 (MMP-7) AND TISSUE INHIBITOR OF METALLOPROTEINASES-2 (TIMP-2) DOWNREGULATION COMPLEMENTS PLAG1 ONCOGENE OVEREXPRESSION IN PLEOMORPHIC ADENOMA PATHOGENESIS [Abstract] [Full text]
Xiaobang PENG, Majid KHAYYATNEZHAD and Leila JOUDI GHEZELJEHMEIDAN
RAPD PROFILING IN DETECTING GENETIC VARIATION IN Stellaria L. (Caryophyllaceae) [Abstract] [Full text]
Juan YIN, Majid KHAYATNEZHAD, Abdul SHAKOOR
EVALUATION OF GENETIC DIVERSITY IN Geranium (Geraniaceae) USING RAPD MARKER [Abstract] [Full text]
Sedigheh Sadat SHARAFI, Pejman AZADI, Maryam JAFARKHANI KERMANI, Vahid ABDOSSI, Ali ESKANDRI
EVALUATION OF DIFFERENT GROWTH HORMONES MEDIATED CALLUS INDUCTION AND REGENERATION AS WELL AS THE EFFECT OF COLCHICINES, ETHYL METHANESULFONATE (EMS) AND GAMMA RADIATION ON SOME TRAITS OF Impatiens walleriana [Abstract] [Full text]
Dezhong BI, Dan CHEN, Majid KHAYATNEZHAD, Zohreh sayyah HASHJIN, Zifa LI, Yuexiang
MOLECULAR IDENTIFICATION AND GENETIC DIVERSITY IN Hypericum L.: A HIGH VALUE MEDICINAL PLANT USING RAPD MARKERS MARKERS [Abstract] [Full text]
Ana VELIMIROVIC, Zoran JOVOVIC, Novo PRŽULJ
Qurban ALI and Arif MALIK
GENETIC RESPONSE OF GROWTH PHASES FOR ABIOTIC ENVIRONMENTAL STRESS TOLERANCE IN CEREAL CROP PLANTS [Abstract] [Full text]
In memoriаm
Dr Milutin Penčić [Text]
Published in „GENETIKA“ Vol. 53, No.1 (2021), pp. 1-10
© 2021 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
UDC 575. 633.1
https://doi.org/10.2298/GENSR2101001T
Orginal scientific article
NBS-LRR-CONTAINING CLASS OF SALICYLIC ACID-INDUCED GENE TRANSCRIPT IN RYE
Motonori TOMITA1, Keiko NAKATSUKA2, Natsuko MORITA2, Evans LAGUDAH3,
Rudi APPELS4
1 Research Institute of Green Science and Technology, Shizuoka University, Shizuoka, Japan
2 Faculty of Agriculture, Tottori University, Tottori, Japan
3 Division of Plant industry, CSIRO, Canberra, Australia
4 BioSciences, The University of Melbourne, Melbourne, Australia
Abstract
Tomita M., K. Nakatsuka, N. Morita, E. Lagudah, R. Appels (2021). NBS-LRR-Containing class of salicylic acid-induced gene transcript in rye. - Genetika, Vol 53, No.1, 1 -10.
NBS-LRR-type disease resistance gene-like cDNA, induced by salicylic acid (SA) was cloned from rye Secale cereale L. (2n = 14 RR) var. Petkus, which has rust resistance genes such as Lr26, Sr31 and Rr9. We designed primers based on the NBS region and performed PCR using Petkus genomic DNA as a template. Next, we TA-cloned a 532-bp DNA fragment containing five homologous amino acid sequences in the NBS region. The SA-treated rye showed strong expression of a transcript of approximately 3.5 knt in the Northern blots probed with the NBS fragment; however, no transcripts were observed with the untreated rye. We constructed a cDNA library of rye var. Petkus treated with SA, and then screened the cDNA library using the TA-cloned NBS fragments as a probe. The entire nucleotide sequence of a full length of rye NBS-LRR-containing class cDNA 3,446 bp was determined.
Keywords: NBS-LRR, Rye, SA, cDNA, gene, structure
Corresponding author: Motonori Tomita, Research Institute of Green Science and Technology, Shizuoka University, Shizuoka, Japan, E-mail: tomita.motonori.k29@kyoto-u.jp
Published in „GENETIKA“ Vol. 53, No. 1 (2021), pp.11-22
© 2021Serbian
Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC
575.633
https://doi.org/10.2298/GENSR2101011K
Original scientific paper
GENOTYPE-BY-ENVIRONMENT INTERACTION FOR SEED YIELD AND OIL CONTENT OF SAFFLOWER (Carthamus tinctorius L.) GENOTYPES
Hasan KOÇ
Bahri Dagdas International Agricultural Research Institute, Konya, Turkey
Abstract
Koç H. (2021). Genotype-by-environment interaction for seed yield and oil content of safflower (Carthamus tinctorius L.) genotypes. - Genetika, Vol 53, No.1, 11-22.
This research was carried out to determine genotype-by-environment interaction of safflower genotypes tested from 2014 to 2017. Konya, where the research was carried out, is the location with the most irregular and the lowest precipitation in Turkey. In this research, the variance analysis over years and genotypes showed that the main effects on genotypes made by year and genotype-by-year interaction were statistically significant (p<0.01) for all characteristics examined. The climatic conditions, especially the amount and distribution of precipitation, over the years allowed genotypes to perform substantially differently in seed yield and oil content of safflower genotypes. The biplot analysis provided significant advantages in identifying the promising genotypes. The genotypes showed similar patterns of performance across the years, while the amount and distribution of precipitation showed similar patterns. The experimental results revealed that the desired genotypes in terms of both stability and high yield, such as Göktürk, G7, and Dinçer, G5 and G9 and oil content, such as Göktürk, Balcı, and Linas, existed. In comparison to oil content, seed yield was more sensitive to environmental factors.
Keywords: GGE (Genotype + Genotype-by-Environment)-biplot, oil content, safflower, seed yield, stability
Corresponding author: Hasan Koç, Bahri Dagdas International Agricultural Research Institute, Konya, Turkey. email: koc175@hotmail.com
Published in „GENETIKA“ Vol. 53, No. 1 (2021), pp.23-49
© 2021 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
https://doi.org/10.2298/GENSR2101023S
Original scientific paper
GENOTYPE BY ENVIRONMENT INTERACTION FOR QUALITY TRAITS
IN CHILLI PEPPER (Capsicum annuum L.)
Tejpal Singh SRAN, S.K. JINDAL* and Neena CHAWLA
Department of Vegetable Science, Punjab Agricultural University, Ludhiana, India.
Abstract
Sran T. S., S.K. Jindal and N. Chawla (2021). Genotype by environment interaction for quality traits in chili pepper (Capsicum annuum L. - Genetika, Vol 53, No.1, 23-49.
There is a need for identifying the specific environments for the selection of adapted and stable genotypes for quality traits in chilli pepper. Among these quality traits, pungency and coloring matter are the most important ones, which need to be in stable amounts in final products. Hence, this multi-environmental evaluation of chilli pepper genotypes was done in three distinct environments, to identify the suitable environments for selection and also suitable genotypes for specific quality traits. The study includes 43 chilli genotypes tested for three distinct growing conditions for nine different quality traits at Punjab Agricultural University and data was analyzed using Eberhart & Russell’s model and GGE Biplot analysis. The environmental effect accounts for more than 35% variation for the capsaicin in oleoresin and dry matter content. While the traits namely capsaicin content in red powder (3%) and capsaicin in green chili (4.73%) were least influenced by the environment. The contribution of G×E interactions was ˂ 25% for all the studied traits except ascorbic acid. The genotype AC 101 was best for capsaicin content in green and red chilli powder across the environments. The data generated from this study help to identify the stable and superior genotypes for quality traits in early, main and late-season planting.
Keywords: chilli, capsaicin, stable, environmental, suitable.
Corresponding author: S.K. Jindal, Department of Vegetable Science, Punjab Agricultural University, Ludhiana, India. phone: +91-8968766600, E-mail: saleshjindal@pau.edu
Published in „GENETIKA“ Vol. 53, No1(2021), pp. 51-64
© 2021 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.633
https://doi.org/10.2298/GENSR2101051C
Original scientific paper
DEVELOPMENT OF LATE TEMPERATE IN VIVO HAPLOID INDUCERS
Rahime CENGİZ *, Mesut ESMERAY
Breeding and Genetics Department, Maize Research Institute, Sakarya, Turkey
Abstract
Cengiz R. and M. Esmeray (2021). Development of late temperate in vivo haploid inducers. - Genetika, Vol 53, No.1, 51-64.
In vivo doubled haploid technique has been widely used in advanced maize breeding programs due to cost, labor and time advantages and increase in efficiency. However, the number of available inducer lines in the world is sufficient. Six BC1 breeding populations including RWS and RWK-76 haploid inducer lines and late temperate ADK-451, ADK-737 and ADK-455 lines were developed by Sakarya Maize Research Institute (MRI) in Turkey. The RWS and RWK-76 haploid inducer lines were used as donors. Pedigree method was employed to develop the inducer lines. Anthocyanin coloration of plant, tassel length, branch number of tassel, plant height, days to flowering, embryo-endosperm colorfulness and haploid induction rate (HIR) were determined. The genotypes with the best characteristics were selected. The families from BC1F3 to BC1F7 were hybridized to liguleless line to determine the HIR and families with HIR over 8% were selected from BC1 populations. The HIR, plant height and days to tassel flowering values of in-1021 and in-1076 candidate haploid inducer lines were 10.5 and 12.3%, 195 and 200 cm, and 69 and 68 days, respectively. The HIR value of RWS donor haploid inducer ranged from 8.9 to 11.3% and for RWK-76 from 7.3 to 9.8%. Simple Sequence Repeats (SSRs) markers were used to identify genetic similarity between late temperate haploid inducer lines and donors. The similarity rates of in-1021 and in-1076 inducer lines to the RWS donor were 38 and 15%, and to the RWK-76 donor were 23 and 27%. The similarity rate between the two candidate inducer lines was 30%. The results indicated that the late temperate haploid inducer lines developed will increase the efficiency of maize breeding.
Keywords: Genetic similarity, haploid inducer line, haploid induction rate, liguleless line, pedigree method.
Corresponding author: Rahime Cengiz, Breeding and Genetics Department, Maize Research Institute, Sakarya, Turkey, email. rcengiz24@gmail.com, Mobile: +905559670353
Published in „GENETIKA“ Vol. 53, No.1 (2021), pp. 65-78
© 2021Serbian
Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2101065Z
Original scientific paper
SNHG7 HAS AN ONCOGENIC ROLE IN COLORECTAL CANCER VIA POTENTIAL SPONGING OF MIR-485-5P AND MIR-193A-5P; IN SILICO APPROACH
Farinaz ZIAEE1, Mohammadreza HAJJARI1*, Seyed Reza KAZEMINEZHAD1, Mehrdad BEHMANESH2
1Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran
2Department of Genetics, Faculty of Biological Science, Tarbiat Modares University, Tehran, Iran
Abstract
Ziaee F., M. Hajjari, S. R. Kazeminezhad, M. Behmanesh (2021). SNHG7 has an oncogenic role in colorectal cancer via potential sponging of MIR-485-5P and MIR-193A-5P; in silico approach. - Genetika, Vol 53, No.1, 65-78.
SNHG7, as a member of the small nucleolar host gene family, is a recently identified long non-coding RNA (lncRNA). Different reports have identified the SNHGs as competing endogenous RNAs (ceRNAs) sponging miRNAs with a role in cancer progression. However, the biological functions of SNHG7 in the colorectal cancer (CRC) remained to be almost unknown. The current in silico study was aimed to find the potential role of SNHG7 in the CRC development. In this study, we showed the up-regulation of SNHG7 as well as its potential correlation with miRNAs, including mir-193a-5p and mir-485-5P. We hypothesized that SNHG7 modulates these miRNAs availability by acting as a molecular sponge. Our findings showed the potential targets of these miRNAs by studying different databases as well as in silico analyses. In summary, we found SNHG7 as a potential ceRNA which may be a promising biomarker for diagnostic and therapeutic target in CRC.
Keywords: colorectal cancer, in silico analyses, long non-coding RNAs, miRNAs, SNHG7
Corresponding author: Mohammadreza Hajjari, Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran. E-mail: mohamad.hajari@gmail.com; m-hajari@scu.ac.ir
Published in „GENETIKA“ Vol. 53, No. 1(2021), pp. 79-92
© 2021 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
https://doi.org/10.2298/GENSR2101079A
Original scientific paper
FORAGE YIELD COMPONENTS OF SOME TOLERANT AND SENSITIVE GRASS PEA GENOTYPE SEEDLINGS AFFECTED BY THE SALINITY STRESS
Seyed Arsalan ASADI1, Alireza POURMOHAMMAD1*, Ali-Asghar ALILOO1,
Khoshnood ALIZADEH2, Mohammad Bagher HASSANPOURAGHDAM3
1Department of Plant Production and Genetics, Faculty of Agriculture, University of Maragheh, Maragheh, Iran
2Dryland Agricultural Research Institute, Maragheh, Iran
3aDepartment of Horticultural Sciences, Faculty of Agriculture, University of Maragheh, Maragheh, Iran
3bIslamic Parliament Research Center, Tehran, Iran
Abstract
Asadi S. A., A. Pourmohammad, A.A. Aliloo, K. Alizadeh, M. B. Hassanpouraghdam (2021). Forage yield components of some tolerant and sensitive grass pea genotype seedlings affected by the salinity stress. - Genetika, Vol 53, No.1, 79 -92.
Grass pea (Lathyrus sativus L.) is relatively tolerant to several abiotic stresses such as salinity. Twenty-five grass pea genotypes provided by the Dryland Agricultural Research Institute, Maragheh, Iran, were exposed to four salinity levels (0, 40, 80, 120 mM). Based on the primary screen, 8 tolerant genotypes and 17 sensitive genotypes were selected. Principal components analysis for the sensitive and tolerant genotypes showed that the first four components explained approximately 71-80% of variance. In PCA, the coefficients associated with leaf showed that, in tolerant genotypes, leaf length and leaf number increased but, leaf width decreased. In other words, salinity stress makes the leaves grow larger and longer, but the width of the leaves decreases and angle of the leaf becomes tighter. So, these traits may be used as an index of salinity tolerance in grass pea selection programs. Cluster analysis based on Ward’s algorithm and Euclidean distance measure effectively separated the tolerant and sensitive genotypes. Cut off dendrogram located tolerant genotypes (except one genotype) in the same subgroup. This fact shows that cluster analysis efficiently divided and/or separated semi-tolerant tolerant genotypes. Shoot dry weight had significant negative correlation with pH, plumule length and plantlet length. Path analysis on tolerant genotypes, revealed the direct effects of leaf number and pod number with shoot dry weight. In stepwise regression, two traits including leaf number and pod number were entered into the model. The results showed that leaf number and pod number had the most direct and positive effect on the shoot dry weight, and the plant height had the highest indirect effect through leaf number on the shoot dry weight. About sensitive genotypes, the results showed that leaf number and fruit fresh weight had the most direct and positive effect on the shoot dry weight, and the plant height had the highest indirect effect through leaf number on the shoot dry weight. Several characteristics were identified to explain shoot dry weight during path analysis. The selections based on the identified characters would be more effective in improving yield in the breeding programs of grass pea.
Keywords: Grass pea, Tolerance to Salinity, PCA, Cluster Analysis
Corresponding author: Alireza Pourmohammad, Department of Plant Production and Genetics, Faculty of Agriculture, University of Maragheh, Maragheh, Iran, E-mail: pourmohammad@ymail.com, Tel: 0098-4137276068
Published in „GENETIKA“ Vol. 53, No.1(2021), pp93-102
© 2021 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
https://doi.org/10.2298/GENSR2101
Original scientific paper
MOLECULAR CHARACTERIZATION OF WILD GRAPES FROM NORTHEASTERN PART OF TURKEY
Muhammed KUPE1, Sezai ERCISLI1, Tatjana JOVANOVIC – CVETKOVIC2*,
Sadiye Peral EYDURAN3, Rayda Ben AYED4
1Department of Horticulture, Faculty of Agriculture, Ataturk University, Erzurum, Turkey
2University of Banja Luka, Faculty of Agriculture, Bosnia & Herzegovina
3Department of Horticulture, Faculty of Agriculture, Igdir University, Igdir, Turkey
4Molecular and Cellular Screening Processes Laboratory, Centre of Biotechnology of Sfax, University of Sfax, Tunisia
Abstract
Kupe M., S. Ercisli, T. Jovanovic - Cvetkovic, S. P. Eyduran, R. B. Ayed (2021). Molecular characterization of wild grapes from northeastern part of Turkey- Genetika, Vol 53, No.1,93 -102.
Progress in grape breeding requires the exploitation of genetic variation among market classes, races and gene pools. Wild grapevines (Vitis vinifera ssp. sylvestris) are being endangered in their natural habitats and high priorities should be given to the wild germplasm.
Turkey is one of the richest sources of wild grapevine and they mostly grown on forest trees on river basin. The present study was carried out to determine the amount of genetic variation and the degree of relatedness among 23 wild grape genotypes using 17 simple-sequence-repeat markers (SSR). Two international grape cultivars, Cabernet Sauvignon and Merlot are also included study. Number of alleles per locus of the 17 Simple Sequence Repeat (SSR) markers ranged from 3.0 to 14.0 and a total of 162 alleles with an average of 9.53 alleles per locus. The average expected and observed heterozygosity values were 0.773 and 0.781, respectively, which exhibited high level of genetic diversity in the wild grape germplasm. The unweighted pair group method with arithmetic mean analysis revealed three main genetic clusters that partially separated wild grape genotypes each other and. The international cultivars formed a out group. The high genetic diversity among native wild grapes from Coruh valley is suggesting that this area could be one of the centre of diversity of the specie. The results indicate a substantial genetic diversity in V. vinifera ssp. sylvestris and the need of exploring a wider area to increase the chance of finding a particular genotype.
Keywords: Wild grape, SSR, molecular characterization, diversity
Corresponding author: Tatjana Jovanovic – Cvetkovic, University of Banja Luka, Faculty of Agriculture, Banja Luka, Bosnia & Herzegovina, e-mail: tatjana.jovanovic-cvetkovic@agro.unibl.org
Published in „GENETIKA“ Vol. 53, No.1 (2021), pp.103-119
© 2021 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2101103B
Original scientific paper
GENETIC DIVERSITY AND SPATIAL STRUCTURE OF THE IMPERILED EUROPEAN POPULATION OF Malus trilobata IN GREECE
Konstantina BALASKA1, George C. ADAMIDIS2, Georgios VARSAMIS1,
Kyriaki PAPALAZAROU1, Paraskevi KARANIKOLA1, Apostolos MANOLIS1,
Athanasios KARAPETSAS3, Nicolas-George ELIADES4, Raphael SANDALTZOPOULOS3, Nikolaos PAPAMATTHAIAKIS1, Kostas POIRAZIDIS5, Georgios KORAKIS1,
Aristotelis C. PAPAGEORGIOU3
1Department of Forestry, Environment and Natural Resources, Democritus University of Thrace, Orestiada, Greece
2Institute of Ecology and Evolution, University of Bern, Bern, Switzerland
3Department of Molecular Biology and Genetics, Faculty of Health Sciences, Democritus University of Thrace, Alexandroupolis, Greece
4Nature Conservation Unit, Frederick University, Nicosia, Cyprus
5Department of Environment, Ionian University, Zakinthos, Greece
Abstract
Balaska K., G.C. Adamidis, G. Varsamis, K. Papalazarou, P. Karanikola, A. Manolis, A. Karapetsas, N.G. Eliades, R. Sandaltzopoulos, N. Papamatthaiakis, K. Poirazidis, G. Korakis, A.C. Papageorgiou (2021). Genetic diversity and spatial structure of the imperiled European population of Malus trilobata in Greece. - Genetika, Vol 53, No.1,103 -119.
Malus trilobata, is a rare tree species occurring in several small and disjunct populations in the eastern part of the Mediterranean basin. The main European population is found in the region of Evros (NE Greece) and is divided in five distinct subpopulations following the species geographical pattern. The genetic diversity of approximately the entire population (69 trees) was analyzed using nuclear microsatellite and random genomic markers. Polymorphism was discovered in 29 out of 45 genomic marker loci (64.44%), while for nuclear microsatellite markers, all three loci were polymorphic with an average of 3.75 alleles per locus. Our results unraveled a specific grouping pattern for both markers. Both genetic markers exhibited relatively low genetic diversity which is in accordance with the prevalent perception that species with fragmented distributions tend to have low genetic diversity, while the differentiation among individuals, revealed a patchy pattern among small groups of trees separated by roads, firebreaks or distance. These results indicate a high genetic fragmentation level for the main European population of M. trilobata, while the presence of roads, firebreaks, conifer plantations and agricultural land, seem to function as potential barriers to gene flow. Consequently, and since it is well-documented that bees hesitate to change foraging patches, as long as their food is abundant, the observed genetic differentiation patterns could be partially attributed to the foraging and flight behavior of bees, which are the main pollinators of the species. The low levels of available genetic diversity combined with the small overall population and repeated events of forest fires inside the M. trilobata distribution, perils the survival of the species and imposes the necessity for a thoroughly organized conservation strategy.
Keywords: Malus trilobata, rare species, fragmentation, pollination, conservation
Corresponding author: Aristotelis C. Papageorgiou, Department of Molecular Biology and Genetics, Faculty of Health Sciences, Democritus University of Thrace, Alexandroupolis, Greece email: apapage@mbg.duth.gr, tel: +30 2551030494
Published in „GENETIKA“ Vol. 53, No. 1(2021), pp. 121-130
© 2021 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
https://doi.org/10.2298/GENSR2101121N
Original
scientific paper
FIRST EVALUATION OF MELON (Cucumis melo. L) LANDRACES UNDER HIGH TUNNEL CONDITION IN SISTAN
Mohammad Reza NAROUI RAD1*and Gholamali KEIKHA2
1Horticulture Crops Research Department, Sistan Agricultural and Natural Resources Research and Education Center, AREEO, Zabol, Iran
2Soil and Water Research Department. Sistan Agricultural and Natural Resources Research and Education Center, AREEO, Zabol, Iran
Abstract
Naroui Rad M. R. and G. Keikha (2021). First evaluation of melon (Cucumis melo. L) landraces under high tunnel condition in Sistan. - Genetika, Vol 53, No.1, 121-130.
In order to evaluate a number of agro-morphological characteristics in 10 melon population, an experiment based on randomized complete block design with three replications was carried out in two years (2017-2018) at high tunnel condition at Zahak Agriculture Research Station. The relationships among the related traits evaluated using by statistical methods. The combined analysis of variance revealed highly significant differences among landraces in evaluated traits. A highly and significant correlation was observed between fruit width and yield (0.81**). Mean comparison using Duncan’s multiple rang test revealed that maximum yield belonged to landrace Zardeivanaki with 29160 kg/ha. Factor analysis was used for understanding the data structure and trait relations. The factor analysis showed that five factors explained 84% of the total variation among the traits. Therefore, the selection may be done according to the first component and it was helpful for a good breeding program for development of high yielding genotypes also landraces Dargazi, Zardeivanaki and Sabzsooski were placed very closely indicating that the responses of these landraces to be similar to high tunnel cultivation condition.
Keyword: landrace, melon, high tunnel, Sistan
Corresponding author: Mohammad Reza Naroui Rad, Horticulture Crops Research Department, Sistan Agricultural and Natural Resources Research and Education Center, AREEO, Zabol, Iran; Email: narouirad@gmail.com; Phone number: +98-9153400109
Published in „GENETIKA“ Vol. 53, No.1 (2021), pp. 131-140
© 2021 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2101131O
Original scientific paper
CHEMOSENSITIZING EFFECTS OF METHYL JASMONATE ON PACLITAXEL-RESISTANT ANDROGEN-DEPENDENT AND INDEPENDENT PROSTATE CANCER CELL LINES
Seda ORENAY-BOYACIOGLU1, Olcay BOYACIOGLU2
1Department of Medical Genetics, Faculty of Medicine, Aydin Adnan Menderes University, Efeler, Aydin, Turkey
2Department of Food Engineering, Faculty of Engineering, Aydin Adnan Menderes University, Efeler, Aydin, Turkey
Abstract
Orenay-Boyacioglu S. and O. Boyacioglu (2021). Chemosensitizing effects of methyl jasmonate on paclitaxel-resistant androgen-dependent and independent prostate cancer cell lines. - Genetika, Vol 53, No.1, 131-140.
The taxane-based therapy provides survival benefit in patients with metastatic prostate cancer; however, the average survival is less than 20 months due to the partial taxane-related chemoresistance. Innovative strategies are needed to overcome the chemoresistance for improved patient survival. In this project, paclitaxel-resistance was developed on androgen-independent PC3 and androgen-dependent 22Rv1 and LNCaP human prostate cancer (PCa) cell lines to investigate the efficacy of methyl jasmonate (MeJa), an anti-cancer drug, in overcoming the chemoresistance. The PCa cell lines were maintained in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS) at 37°C under 5% CO2. The cell lines were exposed to the gradually increasing doses of paclitaxel. Since the resistance on LNCaP could not be achieved, the study was continued with 22Rv1 cell line. It was demonstrated that paclitaxel-resistant cell lines overexpress ABCB1. Resistance levels of cells and MeJa activity in all resistant and parental lines were measured using CellTiter-Glo® luminescent assay. Test results were compared with Student’s t-test or analysis of variance (ANOVA). P≤0.05 (two-tailed) was considered to be significant. In conclusion, MeJa showed more cytotoxicity on paclitaxel resistant PC3 (PC3-PtxR) cells than resistant 22Rv1 (22Rv1-PtxR) cells. Detection of cytotoxic effects of MeJa in overcoming paclitaxel resistance may contribute to the development of alternative new compounds for the prevention or chemosensitization of resistance to chemotherapeutics such as paclitaxel.
Keywords: Prostate cancer, Paclitaxel resistance, Methyl Jasmonate, PC3, 22Rv1
Corresponding author: Seda Orenay-Boyacioglu, Department of Medical Genetics, Faculty of Medicine, Aydin Adnan Menderes University, Efeler, Aydin, Turkey, Tel: +90 539 277 7679, Fax: +90 256 214 6495, E-mail: sorenay@adu.edu.tr
Published in „GENETIKA“ Vol. 53, No. 1(2021), pp. 141-155
© 2021 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.633.11
https://doi.org/10.2298/GENSR2101141W
Original
scientific paper
CLONING AND MOLECULAR CHARACTERIZATION OF TWO NOVEL LMW-m TYPE GLUTENIN GENES FROM Triticum spelta L.
Ruomei WANG1,4, Junwei ZHANG1,4 , Fei LUO1, Nannan LIU1, Slaven PRODANOVIC3*, Yueming YAN1,2*
1College of Life Science, Capital Normal University, Beijing, China
2Hubei Collaborative Innovation Center for Grain Industry (HCICGI),
Yangtze University, Jingzhou, China
3Faculty of Agriculture, University of Belgrade, Belgrade, Serbia
Abstract
Wang R., J. Zhang, F. Luo, N Liu, S. Prodanovic, Y. Yan (2021). Cloning and molecular characterization of two novel LMW-m type glutenin genes from Triticum spelta L.- Genetika, Vol 53, No.1,141 -155.
Spelt wheat (Triticum spelta L., 2n=6x=42, AABBDD), as a hexaploid wheat species, is important sources of food and feed in Europe. It also serves as an important genetic resource for improvement of wheat quality and resistance. In this study, two novel m-type low molecular glutenin subunit (LMW-GS) genes, named as TsLMW-m1 and TsLMW-m2 were cloned by allelic specific polymerase chain reaction (AS-PCR) from German spelt wheat cultivars Rochbergers fruher Dinke and Schwabenkorn, respectively. The complete open reading frames (ORFs) of both genes contained 873 bp encoding 290 amino acid residues, and had typical LMW-GS structural features. Two same deletions with 24 bp at the position of 707-730 bp were present in both genes, while TsLMW-m1 had two nonsynonymous single-nucleotide polymorphism (SNP) variations at the positions of 434 bp (C-A transversion) and 857 bp (G-A transition). Phylogenic analysis revealed that both LMW-m genes were closely related to those from wheat A genome, suggesting that both subunits are encoded by the Glu-A3 locus. Secondary structure prediction showed that TsLMW-m1 and TsLMW-m2 subunits had more α-helices than other wheat LMW-GS including superior quality subunit EU369717, which would benefit to form superior gluten structures and dough properties. The authenticity and expression activity of TsLMW-m1 and TsLMW-m2 genes were verified by prokaryotic expression in E. coli. Our results indicated that two newly cloned TsLMW-m genes could have potential values for wheat quality improvement.
Keywords: Spelt; LMW-GS; Molecular cloning; Phylogenetics; Gluten quality.
Corresponding author: Slaven Prodanovic, Faculty of Agriculture, University of Belgrade, Nemanjina 6, 11080, Belgrade, Serbia, phone: +381638687246, fax: +381113161987, E-mail: slavenp@agrif.bg.ac.rs
Yueming Yan, College of Life Science, Capital Normal University, Beijing, Xisanhuan Beilu 105, 100048 Beijing, China, phone: +861068902777, fax: +861068902777, E-mail: yanym@cnu.edu.cn
Published in „GENETIKA“ Vol. 53, No.1(2021), pp157-166
© 2021 Serbian
Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
https://doi.org/10.2298/GENSR2101157R
Original scientific paper
CORRELATION AND PATH ANALYSIS OF YIELD AND YIELD COMPONENTS
IN WINTER RAPESEED
Velimir RADIĆ, Igor BALALIĆ, Miloš KRSTIĆ, Ana MARJANOVIĆ-JEROMELA
Institute of Field and Vegetable Crops, Novi Sad, Serbia
Abstract
Radić V., I. Balalić, M. Krstić, A. Marjanović-Jeromela (2021). Correlation and path analysis of yield and yield components in winter rapeseed. - Genetika, Vol 53, No.1, 157-166.
Five different rapeseed genotypes were tested. The following traits were considered: seed yield, seed germination, seed oil content, 1000 seed weight and seed protein content and they were used to determine whether there is significant or nonsignificant correlation. Highest average results were determined in genotype G-3 (yield 2.544 kg; germination 90%; 1000 seed weight 4,30 g; oil content 45,31% and protein content 19,83%) while G-5 (1.838 kg; 87%; 3,50 g; 44,77% and 17,28%) had the lowest average result of observed parameters. Highly significant positive correlations were found while comparing seed yield with 1000 seed weight (0.753**) and protein content (0.726**). High significant positive correlation were also determined comparing seed germination with 1000 seed weight (0.832**) and protein content (0.892**). Also high significant correlation was determined comparing 1000 seed weight and protein content (0.812**). Positive significant correlations were found by comparing seed yield and seed germination (0.644*). Path analysis indicated highest significant positive direct effect of 1000 seed weight (0.716**) and protein content (0.666**) on seed yield. High but negative significant value had seed germination (-0.645**). In the study of indirect effects on seed yield, none of significant effects were determined. The study of direct effects on oil content showed that the seed germination had high significant positive effect (-1.296**) on oil content. Significant but hot high effect has seed yield (0.556*). In other two traits it was determined negative high significant effects (1000 seed weight – 0.797** and protein content – 0.717**) on seed oil content. The existence of indirect significant effects on oil content in four cases was determined.
Keywords: rapeseed, yield, oil, correlation, path analysis
Corresponding author: Velimir Radić, Institute of Field and Vegetable Crops, Maksima Gorkog 30, Novi Sad 21000, Serbia, email: velimir.radic@ifvcns.ns.ac.rs.
Published in „GENETIKA“ Vol. 53, No. 1(2021), pp. 167-180
© 2021Serbian
Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575. 633
https://doi.org/10.2298/GENSR2101167C
Original scientific paper
MORPHOLOGIC AND CYTOPLASMIC ASSESSMENTS OF BULB ONION
(Allium cepa L.) LANDRACES
Esra CEBECI1*, Huseyin PADEM2, Ali Fuat GOKCE3
1Bati Akdeniz Agricultural Research Institute, 07800, Antalya-Turkey
2Isparta University of Applied Sciences, Department of Horticulture, 32260, Isparta- Turkey
3Omer Halisdemir University, Faculty of Agricultural Sciences and Technologies, 51240, Nigde-Turkey
Cebeci E., H. Padem, A. F. Gokce (2021). Morphologic and cytoplasmic assessments of bulb onion (Allium cepa L.) landraces. - Genetika, Vol 53, No.1,167 -180.
Today climate change threatens to reduce crop yield and harming the food security. Local landraces have adaptation skills to shifting climatic conditions. Using of this local source in plant breeding programs becoming an alternative strategy. In this study, 97 landraces were collected to initiate the bulb onion breeding program eligible for the current trends. Collected materials were morphologically characterized using 21 descriptors, derived from UPOV (International Union for the Protection of New Varieties of Plants). Clustering which was conducted by the NTSYS (Numerical Taxonomy and Multivariate Analysis System) program using UPGMA (Unweighted Pair Group Method of Arithmetic Averages) method, showed that the genetic similarity rate of the landraces was calculated between 0.06-0.96. Hybrid onion breeding program depends on the cytoplasmic-genic male sterility (CMS) system. Thus, the PCR-markers were applied to identify the cytoplasm types of the landraces. Among landraces N-cytoplasm was found in 78 accessions and S-cytoplasm was found in 19 accessions. At the end of the study, a qualified gene pool has been established consisted of characterized onion genotypes which will might be used in further breeding studies.
Keywords: characterization, CMS, diversity, NTSYS, onion breeding,
Corresponding author: Esra Cebeci, Bati Akdeniz Agricultural Research Institute, 07800, Antalya-Turkey, email; esrac3@hotmail.com, Phone:+90 532 5833701
Published in „GENETIKA“ Vol. 53, No.1(2021), pp. 181-194
© 2021 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.633.11
https://doi.org/10.2298/GENSR2101181T
Original scientific paper
PHENOTYPIC AND MOLECULAR DIVERSITY OF WHEAT SPECIES (Triticum spp.) IN RELATION TO PLANT HEIGHT AND HEADING TIME
Verica TAKAČ, Ankica KONDIĆ-ŠPIKA, Dragana TRKULJA, Ljiljana BRBAKLIĆ,
Vesna ŽUPUNSKI, Vladimir AĆIN, Sanja MIKIĆ
Institute of Field and Vegetable Crops, Novi Sad, Serbia
Abstract
Takač V., A. Kondić-Špika, D. Trkulja, Lj. Brbaklić, V. Župunski, V. Aćin, S. Mikić (2021). Phenotypic and molecular diversity of wheat species (Triticum spp.) in relation to plant height and heading time. - Genetika, Vol 53, No.1, 181-194.
Plant height and heading time are important agronomic traits that significantly contribute to the improvement of wheat adaptability and yield. The aim of this study was to evaluate the level of phenotypic variability of these two traits in a collection of wheat species originating from 20 countries, to analyse its molecular diversity based on the microsatellite loci associated with the previously mapped quantitative trait loci, and to estimate potential of microsatellites to detect polymorphism in different wheat species and reveal allelic patterns in relation to the geographical origin. The significant differences in plant height and heading time among different wheat groups were observed, while the differences in means among three different growing seasons were significant only for heading time. The principal coordinate analysis distinguished wheat genotypes by their origin and ploidy level. Wheat varieties from America, South and Southeast Europe, and West and Central Europe had the highest molecular diversity, as was evidenced by the higher number of alleles, number of group-specific alleles, Shannon's information index and gene diversity. The Nei’s genetic identity indicated genetic similarity of geographically distinct groups, such as South and Southeast Europe and Russian (0.901) and South and Southeast Europe and American genotypes (0.638). The studied collection with high observed level of both phenotypic and molecular diversity for plant height and heading time may be a valuable source of variation for wheat breeders to fine adjust these traits to achieve better agronomic performance in certain local environments.
Keywords: diversity, plant height, heading time, microsatellites, wheat
Corresponding author: Verica Takač, Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad, Serbia, Phone: 0214898214, e-mail: verica.takac@ifvcns.ns.ac.rs
Published in „GENETIKA“ Vol. 53, No. 1(2021), pp. 195-208
© 2021 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
https://doi.org/10.2298/GENSR2101195S
Original scientific paper
TRANSFERABILITY OF APPLE AND PEAR SSRS TO OTHER TEMPERATE POME FRUIT CROPS OF FAMILY ROSACEAE
Himani SHARMA, Parul SHARMA and Rajnish SHARMA*
Department of Biotechnology, Dr YS Parmar University of Horticulture & Forestry, Nauni, Solan, India
Abstract
Sharma H., P. Sharma, R. Sharma (2021). Transferability of apple and pear SSRS to other temperate pome fruit crops of family Rosaceae. - Genetika, Vol 53, No.1, 195-208.
Extensive use of simple sequence repeat (SSR) is facilitated if loci would be transferable across species even in closely related genera to overcome high cost and efforts involved in their development as major constraints. In the present study, apple and pear genomic microsatellite primer pairs were used to amplify SSR loci in apple, pear, quince and loquat genotypes, respectively. Already reported SSRs were selected based on their polymorphic survey for successful amplification with at least one polymerase chain reaction (PCR) product of the approximate size expected for a homologous locus screened among apple and pear genotypes for further transferability exploration across other temperate pome fruit crops, respectively. Highest transferability of apple and pear SSR, 61.53 % and 73.33 % was observed in closely related quince and apple genotypes, respectively. This indicated that primer binding sites between these two closely related genera, Malus and Pyrus, are fairly well conserved. Maximum transferability rate was found to be 93.33 % and 80.00 % across all the subjected genotypes for primer CH05D11 and TSUenh016 in apple and pear, respectively. The transferability of markers is based on genomic similarity, and can reflect the relationship of genome collinearity and even evolution between species. This high level of transferability of apple and pear SSRs to other temperate pome fruit crops indicated their promise for application to future molecular screening, map construction, and comparative genomic studies, etc.
Keywords: apple, pear, SSR, temperate pome fruit crops, transferability, markers.
Corresponding author: Rajnish Sharma, Department of Biotechnology, Dr YS Parmar University of Horticulture & Forestry, Nauni, Solan (HP) 173 230 India, Phone: 01792252310, Fax: 01792252844, E-mail: rajnish.sharma@yahoo.co.in
Published in „GENETIKA“ Vol. 53, No. 1(2021), pp. 209-218
© 2021 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2101209T
Original scientific paper
GENETICAL INFLUENCE ON HISTOLOGICAL STRUCTURE AND CHEMICAL COMPOSITION OF MUSCULAR TISSUE IN SHEEP
Vladimir Ivanovich TRUKHACHEV1*, Marina Ivanovna SELIONOVA1, Irina Ivanovna DMITRIK2, Milan P. PETROVIC3, Violeta Caro PETROVIC3, Dragana RUZIC-MUSLIC3, Nevena MAKSIMOVIC3
1Russian State Agrarian University – Moscow Timiryazev Agricultural Academy, Moscow, 127550, Russia
2FGBNU All-Russian Research Institute of Sheep and Goat breeding, 355015, Stavropol, Russia
3Institute for Animal Husbandry, Zemun, Belgrade, Serbia
Abstract
Trukhachev V. I., M. I. Selionova, I. I. Dmitrik, M.P. Petrovic, V. Caro Petrovic, D. Ruzic-Muslic, N. Maksimovic (2021). Genetical influence on histological structure and chemical composition of muscular tissue in sheep. - Genetika, Vol 53, No.1, 209-218.
The effect of different genotypes kept in the same feeding level on microstructure and chemical composition of muscular tissue (musculus longissimus dorsi) of sheep has studied. The study aims to see how the gene combination of different breeds influences the mentioned problem. It was revealed that sheep muscular tissue obtained by crossing the North-Caucasian breeds (NC) and Texel (T) was characterized by a larger quantity of muscular fibres by 4.73 % and 10.14% (P<0.05), and a smaller diameter by 5.65 % and 18.6% (P< 0.05), a higher evaluation of marbling by 5.2 and 8.3 points, less connective tissue content by 0.8 and 1.3 absolute percentage compared to thoroughbred North-Caucasian and from crossing North-Caucasian with Poll Dorset (PD). It showed that meat obtained from crossing animals of the T×NC variant is more delicate, succulent, and has a total higher quality and consumer properties.
The results of different crossing indicate the reasonability to use morphological characteristics of muscular tissue on the histological level when the quality of sheep slaughtering performance had evaluated.
Keywords: genotype, crossbreeding, sheep slaughtering performance, meat quality, micro-structural analysis, chemical composition
Corresponding author: Vladimir Ivanovich Trukhachev Russian State Agrarian University – Moscow Timiryazev Agricultural Academy, Moscow, 127550, Russia E-mail: m_selin@mail.ru
Published in „GENETIKA“ Vol. 53, No. 1(2021), pp.219-233
© 2021 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
https://doi.org/10.2298/GENSR2101219M
Original scientific paper
HEAT-INDUCED ACCUMULATION OF PROLINE AND YIELD COMPONENTS
IN GENETICALLY DIVERGENT CEREAL VARIETIES
Stefan M. MARKOVIĆ1*, Desimir KNEŽEVIĆ2, Nenad M. NEŠOVIĆ3,
Nevena H. DJUKIĆ1
1 University of Kragujevac, Faculty of Science, Department of Biology and Ecology, Kragujevac, Serbia
2 University of Priština, Faculty of Agriculture, Kosovska Mitrovica, Lešak, Serbia
3 Agricultural Extension Service Kraljevo Ltd., Kraljevo, Serbia.
Abstract
Marković S., D. Knežević, N. Nešović. N. Djukić (2021). Heat-induced accumulation of proline and yield components in genetically divergent cereal varieties. - Genetika, Vol 53, No.1, 219 -233.
Growth and development of cereals is affected by abiotic factors, particularly by high temperature. An important factor in plant adaptation to abiotic stress can be the proline accumulation. Proline is an amino acid involved in a series of metabolic processes and is important as a protein stabilizer, osmolyte and antioxidant. The aim of this experiment was to determine the effect of high temperature on the proline content and yield elements in different cereal varieties during two vegetative seasons. For the investigation, plant material of 8 genetically divergent winter wheat varieties, 1 variety of triticale and 1 oat variety were used. Samples were collected in days with moderate midday air temperatures of 24-26°C in the milk stage and after a few days in the same phenological stage, in conditions of high midday air temperatures of 34-36°C. Proline accumulation was determined spectrophotometrically. Statistical analyses of data were done in the SPSS program. The results during the first experimental year showed that under moderate air temperature conditions proline content was 0.661 µmol g-1 fresh plant, and in the second experimental year 0.777 µmol g-1 fresh plant. Under conditions of heat stress during the first year, the content of proline increased to 2.169 µmol g-1 fresh plant, and in the second experimental year the average content was 2.510 µmol g-1 fresh plant, which confirms the increase of proline accumulations under heat stress. Compared with other cereal varieties, wheat varieties Zvezdana, Pobeda, Simonida and Avenu were characterized by higher contents of proline in conditions of heat stress in both vegetative seasons. Statistical analysis and correlation of the results of proline accumulation and yield elements showed that more resistant varieties to heat stress conditions have higher yields.
Keywords: proline, heat stress, cereals, yield, genetic variability
Corresponding author: Stefan Marković, University of Kragujevac, Faculty of Science, Department of Biology and Ecology, Radoja Domanovića 12, 34000, Kragujevac, Serbia. E-mail: stefan.markovic@pmf.kg.ac.rs; Phone: +381 34 336223.
Published in „GENETIKA“ Vol. 53, No. 1(2021), pp.235-246
© 2021 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2101235P
Original scientific paper
POPULATION GENETICS IN MULTIPLE SCALES: GENETIC MICROSTRUCTURE OF A CAT POPULATION IN COLOMBIA
Mauricio PEÑUELA1, Angie PATIÑO-MONTOYA2, Andrés PEÑA-CRUZ3,
Heiber CÁRDENAS4
1Posgrado en Ciencias-Biología, Facultad de Ciencias Naturales y Exactas, Universidad del Valle, Cali-Colombia
2 Programa de Pós-graduação em Ecologia, Doutorado em Ecologia, Universidade de Brasília, Brasília D.F.-Brasil
3Gobernación del Valle del Cauca, Colombia
4Departamento de Biología, Facultad de Ciencias Naturales y Exactas, Universidad del Valle, Cali-Colombia
Abstract
Peñuela M., A. Patiño-Montoya, A. Peña-Cruz, H. Cárdenas (2021). Population genetics in multiple scales: genetic microstructure of a cat population in Colombia- Genetika, Vol 53, No.1, 235-246.
Considering of multiples research of genetic in cats, the objective of this study was to compare the genetic diversity of the domestic cat population at different spatial and temporal scales. According to the administrative division of the city of Cali, 8 colonies (localities) were chosen, which together form 5 subpopulations within the city for sampling. The phenotype from each individual’s coat was recognized for the subsequent calculation of allele frequencies, Hardy-Weinberg (HW) equilibrium, genetic structure, and correlated genetic diversity and structure with antiquity in each neighborhood. The non-agouti allele obtained the highest frequencies, and the white allele had the lowest frequencies in all colonies and subpopulations; the manx allele was also reported. HW equilibrium was found in the orange locus, except for the Salomia, Sena and Santa Barbara colonies of the NW subpopulation. A significant association was found between the diversity of the tabby locus and antiquity and the differentiation of the colonies (FCT) with antiquity of colonies. In conclusion, domestic cats in the city of Cali behave as a single population, with incipient genetic microstructure phenomena, which are part of the natural dynamics of the population in their interaction with the urban environment.
Keywords: Felis catus, dispersion, population genetics.
Corresponding author: Mauricio Peñuela, Posgrado en Ciencias-Biología, Facultad de Ciencias Naturales y Exactas, Universidad del Valle, Cali-Colombia, Email: mauricio.penuela@hotmail.com
Published in „GENETIKA“ Vol. 53, No. 1(2021), pp. 247-262
© 2021 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2101247I
Original scientific paper
GENETIC DIVERSITY OF Pseudomonas syringae PV. syringae ISOLATED FROM SWEET CHERRY IN SOUTHERN AND NORTHERN REGIONS IN SERBIA
Renata ILIČIĆ1, Tatjana POPOVIĆ2*, Sanja MARKOVIĆ3, Aleksandra JELUŠIĆ3,
Ferenc BAGI1, Slobodan VLAJIĆ4, Slaviša STANKOVIĆ5
1University of Novi Sad, Faculty of Agriculture, Novi Sad, Serbia
2Institute for Plant Protection and Environment, Belgrade, Serbia
3University of Belgrade, Institute for Multidisciplinary Research, Belgrade, Serbia
4 Institute of Field and Vegetable Crops, Novi Sad, Serbia
5 University of Belgrade, Faculty of Biology, Belgrade, Serbia
Abstract
Iličić R., T. Popović, S. Marković, A. Jelušić, F. Bagi, S. Vlajić, S. Stanković (2021). Genetic diversity of Pseudomonas syringae pv. syringae isolated from sweet cherry in southern and northern regions in Serbia.- Genetika, Vol 53, No.1, 247 -262.
Bacterial canker and leaf spot caused by plant pathogenic bacterium Pseudomonas is among the most destructive cherry diseases worldwide. Nowadays in Serbia, sweet cherry production significantly increased and the new plantations, mainly grown from imported planting material are being raised every year. During spring, 2018 and 2019, occurrence of bacterial canker and leaf spot symptoms was observed on a newly planted sweet cherry plantations in two localities, Žitorađa (Southern region) and Karavukovo (Northern region-Vojvodina). Typical P. syringae colonies were isolated on Nutrient Sucrose Agar supplemented with 5% sucrose (NSA). A total of fifteen isolates were selected and identified. Results of the LOPAT test (+---+) determined them to belong to fluorescent Pseudomonas Group Ia, while results of G+A+T-Ta- tests indicate presence of Pseudomonas syringae pv. syringae. Pathogenicity was confirmed on immature sweet and sour cherry fruitlets by forming of black, sunken lesions for all tested isolates. Genes syrB and syrD were successfully detected in all tested isolates. DNA sequencing using gapA, gltA, gyrB and rpoD housekeeping genes determined tested isolates to belong to P. s. pv. syringae using the National Center for Biotechnology Information (NCBI) nucleotide BLAST. The Serbian isolates shared 99.47% to 100% (Žitorađa) and 99.38% to 100% (Karavukovo) identity with bacterium P. s. pv. syringae. Phylogenetic analysis grouped isolates from Žitorađa in one tree cluster, separate from the Karavukovo isolates, indicating presence of two genetically diverse groups of causal pathogen P. s. pv. syringae, obtained from two geographically distinct localities in Serbia. Phylogeographic analysis grouped isolates from Žitorađa in multilocus haplotype coded as REz and isolates originated from Karavukovo in multilocus haplotype coded as REk. Considering that during last few years P. syringae continuously occurs mainly in young sweet cherry plantations, where imported material is used for raising, health status check is recommended to be included as obligatory measure when nursery material is used from import.
Keywords: bacterium, bacterial canker, leaf spot, sweet cherry, plantation
Corresponding author: Tatjana Popović, Institute for Plant Protection and Environment, Teodora Drajzera 9, Belgrade, Serbia, email: tanjaizbis@gmail.com, Phone: 00381112660049, Fax: 00381112669860
Published in „GENETIKA“ Vol. 53, No. 1(2021), pp. 263-270
© 2021 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2101263N
Original scientific paper
THE INFLUENCE OF GENETIC β -LACTOGLOBULIN POLYMORPHISM ON THE QUANTITY AND QUALITY OF MILK OF THE SIMMENTAL BREED IN SERBIA
Dragan NIKŠIĆ1*, Vlada PANTELIĆ1, Dušica OSTOJIĆ ANDRIĆ1, Dragan STANOJEVIĆ2, Nikola DELIĆ1, Aleksandar STANOJKOVIĆ1, Maja PETRIČEVIĆ1
1 Institute for Animal Husbandry, Belgrade-Zemun, Republic of Serbia
2 Faculty of Agriculture, University of Belgrade, Belgrade-Zemun, Republic of Serbia
Abstract
Nikšić D., V. Pantelić, D. Ostojić Andrić, .D. Stanojević, N. Delić, A. Stanojković, M. Petričević (2021). The influence of genetic β -lactoglobulin polymorphism on the quantity and quality of milk of the Simmental breed in Serbia.- Genetika, Vol 53, No.1, 263-270.
The study of the link between genes controlling protein polymorphism and milk performance traits of domestic animals has great economic importance from a selection point of view, as it reduces the generation interval, leading to increased productivity in livestock. The objective of this paper was to establish the influence of genetic β -lactoglobulin polymorphism on the quantity and quality of milk of the simmental breed in Serbiа. For the research blood samples were taken from a total of 157 Simmental cows. The genotypes of Simmental cows for β-lactoblobulin and their effect on quantitative milk performance traits were determined using the PCR-RFLP analysis. The variability of traits influenced by the genetic polymorphism of β-lactoblobulin was statistically very highly significant (p <0.0001) for milk yield in standard lactation and milk fat and protein yields, while it showed no statistically significant variability (p> 0.05) for content of milk fat and protein. The AB genotype cows achieved a 121 kg and 338 kg increase in milk production compared to the BB genotype and AA genotype cows, respectively.
Keywords: polymorphism, β-lactoblobulin, PCR-RFLP, Simmental breed
Corresponding author: Dragan Nikšić, Institute for Animal Husbandry, Belgrade-Zemun, Republic of Serbia,email: draganniksic84@gmail.com
Published in „GENETIKA“ Vol. 53, No. 1(2021), pp. 271-282
© 2021 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2101271W
Original scientific paper
SEQUENCE ANALYSES OF INSULIN-LIKE GROWTH FACTOR 1 GENE
IN NIGERIAN INDIGENOUS AND ARBOR ACRE CHICKENS
M. WHETOa*, O.O, ISMAILAa, M.A. ADELEKEb, A.S. ADENAIKEa, S.O. PETERSc,
A. YAKUBUd, A.O. ADEBAMBOa, C.O.N. Ikeobia and O.A. ADEBAMBOa
aDepartment of Animal Breeding and Genetics, Federal University of Agriculture, Abeokuta, Nigeria
bDiscipline of Genetics, School of Life Sciences, University of KwaZulu-Natal (Westville Campus), South Africa
cDepartment of Animal Science, Berry College, Mount Berry, GA 30149, USA
dDepartment of Animal Science, Faculty of Agriculture, Nasarawa State University, Keffi, Shabu-Lafia campus, Lafia, Nigeria
Abstract
Wheto M., O.O, Ismaila, M.A. Adeleke, A.S. Adenaike, S.O. Peters, A. Yakubu, A.O. Adebambo, C.O.N. Ikeobi and O.A. Adebambo (2021). Sequence analyses of insulin-like growth factor 1 gene in Nigerian indigenous and Arbor acre chickens. - Genetika, Vol 53, No.1, 271 -282.
The chicken Insulin-like growth factor 1 (IGF1) is a candidate gene for growth, body composition and metabolism, skeletal characteristics and growth of adipose tissue and fat deposition in chickens. It is mapped to 165.95 cM on chromosome 1 and composed of four exons and three introns, spanning more than 50 kb. Genomic DNA was extracted from blood samples collected from the experimental birds using Qiagen DNA extraction kits. Polymersae chain reaction (PCR) was carried out using established primers. The PCR amplicon involving 5’untranslated region were sequenced. The sequences were analysed to identify polymorphisms, their genetic diversities and evolutionary relationships among three strains of Nigerian indigenous chickens [Frizzle Feathered (7), Normal Feathered (19) and Naked Neck (19), and the Arbor Acre broiler chicken (17)]. Nucleotide sequences generated were edited and aligned using Codon Code Aligner. Diversity analysis was done using DnaSp while MEGA6 software was used to plot phylogenetic tree using maximum likelihood method. A total of nineteen single nucleotide polymorphisms (SNPs) were detected from 560 bp portions of the 5’UTR among the four chicken populations studied with none detected in the Frizzle feathered chicken. The Naked neck chicken had the highest number of SNP’s (13), haplotypes (6), haplotype diversity (0.778), nucleotide diversity (0.00487), average number of nucleotide differences (2.725), highest number of polymorphic (segregating) sites (13), parsimony informative site (5) and singleton variable site (8). The Naked neck chicken therefore had the highest rate of mutation and degree of allelic variation compared to other chicken strains used in this study. The phylogenetic tree showed that small genetic differentiation exists among the chicken populations studied. Some of the SNPs are newly discovered; hence, association between these alleles and productive traits in Nigerian native chickens is desirable in future studies.
Keywords: IGF1, FUNAAB Alpha, Indigenous chicken, Gene, Sequence.
Corresponding author: M. Wheto, Department of Animal Breeding and Genetics, Federal University of Agriculture, Abeokuta, Nigeria, E-mail: whetom@funaab.edu.ng; +2348038453793
Published in „GENETIKA“ Vol. 53, No1(2021), pp.283-294
© 2021 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
https://doi.org/10.2298/GENSR2101283V
Original scientific paper
CRYOPRESERVATION OF SERBIAN AUTOCHTHONOUS PLUM ‘CRVENA RANKA’ USING ALUMINIUM CRYO-PLATES
Tatjana VUJOVIĆ*, Darko JEVREMOVIĆ, Tatjana MARJANOVIĆ, Đurđina RUŽIĆ
Fruit Research Institute, Čačak, Republic of Serbia
Abstract
Vujović T., D. Jevremović, T. Marjanović, Đ. Ružić (2021). Cryopreservation of Serbian autochthonous plum ‘Crvena Ranka’ using aluminium cryo-plates. - Genetika, Vol 53, No.1, 283 -294.
ʻCrvena Rankaʼ is one of the oldest autochthonous plum cultivars in Serbia. However, it is endangered due to the long-term pathogen pressure and continuous suppression by commercially important cultivars and therefore faced with genetic erosion. This study was carried out to investigate the suitability of two efficient and simple cryopreservation methods using aluminium cryo-plates for its conservation. Precultured shoot tips of this genotype were embedded in alginate gel into cryo-plates wells. Osmoprotection was performed using loading solution with 1.9 M glycerol and 0.5 M sucrose (30 min at room temperature). In the V cryo-plate protocol, explants were dehydrated at room temperature for 20 or 40 min with PVS A3 (37.5% glycerol, 15% dimethylsulfoxide, 15% ethylene glycol and 22.5% sucrose) or with PVS3 (50% glycerol and 50% sucrose) for 60 min. In the D cryo-plate protocol, desiccation for 2, 2.5 or 3 h was performed over silica gel. Then, the cryo-plates were directly immersed into liquid nitrogen. Unloading was done in MS medium containing 0.8 M sucrose (30 min at room temperature). In the V cryo-plate procedure regrowth of cryopreserved explants dehydrated with PVS A3 was between 50% and 51.9%, while in those dehydrated with PVS3 it was 66.7%. As for the D cryo-plate method, regrowth of cryopreserved explants ranged between 30–40%. After regrowth, shoots were successfully multiplied and rooted. Results prove the feasibility of these new cryogenic methods for a long-term storage of this valuable Prunus genotype.
Keywords: Prunus domestica L., plant vitrification solution, V cryo-plate, desiccation, D cryo-plate
Corresponding author: Tatjana Vujović, Fruit Research Institute, Kralja Petra I/9, 32000 Čačak, Republic of Serbia, Phone: +381 32 321 375, Fax: +381 32 321 391, E-mail: tvujovic@institut-cacak.org
Published in „GENETIKA“ Vol. 53, No. 1(2021), pp. 295-303
© 2021 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
https://doi.org/10.2298/GENSR2101295U
Original
scientific paper
IN VITRO PROPAGATION OF BLACK CUMIN (Nıgella satıva L.) PLANTS
Hüseyin UYSAL
Aydın Adnan Menderes University, Agricultural Faculty, Department of Agricultural Biotechnology, Aydın, Turkey
Abstract
Uysal H.(2021). In vitro propagation of black cumin (Nıgella satıva L.) plants. - Genetika, Vol 53, No.1, 295 -303.
This study was carried out to determine in vitro development using Black cumin leaf and stem explants. Çameli black cumin variety was used as plant material. Five different nutrient mediums (1. LS2.5, 2. MS, 3. MS + 0.5 mg.l-1 IAA, 4. MS + 0.5 mg.l-1 BAP, 5. MS + 0.5 mg.l-1 IAA + 0.5 mg.l-1 BAP) containing 30 g sugar were used in this study. As a result of the research, 100% callus formation was detected in the stem explants cultured in the number 1 and number 5 mediums. These were followed by stem explants cultured in medium 4 with a success rate of 96%. Of this rate, 66% was shoot formation, and 30% was callus formation. Direct shoot regeneration was performed only on stem explants cultured in mediums 4 and 3, with a 66% success rate in medium four and a 36% success rate in medium 3. The highest plant regenerations from calluses were gained from stem explants (273.3%) in medium 4, followed by calluses gained from leaf explants (262.5%) in the same medium. These were followed by cultures in medium 3, with calluses derived from stem explants (255%) and leaf explants (150%). No plant regeneration was determined from calluses gained in the medium 1. Thus it is evident that high auxin content and auxin-cytokinin balanced mediums encouraged callus formation in the black cumin plants. The addition of only IAA or BAP to the medium promoted shoot formation in the stem explants, but direct shoot regeneration was not thereby achieved from the leaf explants. These results show that, for in vitro clonal propagation studies done on black cumin plants, a high auxin containing medium is preferable if the aim is callus formation. If the aim is direct shoot regeneration, BAP or other cytokinin-containing medium is preferred.
Keywords: Black cumin, clonal propagation, hormone, in vitro, Nigella sativa
Corresponding author: Hüseyin Uysal, Aydın Adnan Menderes University, Agricultural Faculty, Department of Agricultural Biotechnology, Aydın, Turkey, Phone: +90 256 218 2000 Ex: 6430, e-mail: hüseyin.uysal@adu.edu.tr
Published in „GENETIKA“ Vol. 53, No. 1(2021), pp 305-322
© 2021 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
https://doi.org/10.2298/GENSR2101305A
Original scientific paper
THE EFFECT OF YEAR AND GENOTYPE ON PRODUCTIVITY AND QUALITY
OF POTATO
Sanida ARSLANOVIĆ-LUKAČ1, Nenad ĐURIĆ1, Veselinka ZEČEVIĆ1,
Jasmina BALIJAGIĆ2, Dobrivoj POŠTIĆ3
1Megatrend University, Faculty of Biofarming, Bačka Topola, Serbia
2University of Montenegro, Biotechnical Faculty Podgorica, Montenegro
3Institute for Plant Protection and Environment, Belgrade, Serbia
Abstract
Arslanović-Lukač S., N. Đurić, V. Zečević, Jasmina Balijagić, D. Poštić (2021). The effect of year and genotype on productivity and quality of potato. - Genetika, Vol 53, No.1, 305-322.
The study presents results of a three-year experiment of variability of different potato varieties in Montenegro: early maturity varieties (Riviera), medium early (Almera, Aladin and Bounty) and medium late (Agria, Margarita, Kennebec and Desiree). The research was conducted during 2015, 2016 and 2017, in acid, brown soil and three different climatic locations: Nedakusi (556 m a.s.l.), Sutivan (680 m a.s.l.) and Orahovica (900 m a.s.l.). Field experiments were set up using standard methodology in random block design in three repetitions. The analysis of variance suggest that number of tubers per plant, mean tuber weight, yield of small tubers, marketable and total yields were significantly fluctuating depending on genotype (G), year (Y) and the location (L). In addition to individual influences of different factors, their interactions were also pronounced (G x Y, G x L, Y x L, G x Y x L). In the three-year period average, the highest total yield was recorded in Nedakusi (31.41 t ha-1), followed by Sutivan (21.35 t ha-1), while the lowest average yield (17.36 t ha-1) was recorded in Orahovica. As expected, on the three-year average, the highest percentage of dry matter was found in late varieties Agria - 25.46%, then Desiree - 25.33%, followed by Kennebec with 25.13%, while the lowest percentage of dry matter was found in medium early variety Almera - 20.82%. The highest average yield of tubers in the three-year period was recorded in the Aladin variety, followed by Agria and Desiree, while the lowest average yield was recorded in the variety Riviera. Obtained results show that the highest yields over observed locations were recorded in medium early varieties that formed high number of tubers per plant (Aladin) and medium late varieties (Desiree and Agria) give satisfactory and stable yields.
Keywords: potato, genotype, productivity, dry matter, starch
Corresponding author: Dobrivoj Poštić, Institute for Plant Protection and Environment, Belgrade, Serbia E-mal: pdobrivoj@yahoo.com
Published in „GENETIKA“ Vol. 53, No.1(2021), pp. 323-338
© 2021 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-ZemunSerbia
UDC 575.633
https://doi.org/10.2298/GENSR2101323P
Original scientific paper
Vesna PERIĆ, Mirjana SREBRIĆ, Ana NIKOLIĆ, Danijela RISTIĆ,
Snežana MLADENOVIĆ DRINIĆ, Zoran DUMANOVIĆ
Maize Research Institute “Zemun Polje”, Belgrade, Serbia
Abstract
Perić V., M. Srebrić, A. Nikolić, D. Ristić, S.Mladenović Drinić, Z. Dumanović (2021). Stability of yield and seed composition in early maturing soybean genotypes assessed by AMMI analysis.- Genetika, Vol 53, No.1,323 -338.
Identification of stable sources for breeding for important agronomic traits is prerequisite for providing a continuous and long-term progress in breeding. In this study, thirty-one early-maturing genotypes from soybean collection of Maize Research Institute “Zemun Polje” were evaluated across four environments (two years and two locations) according to randomized complete block design with three replications. The aim of research was to examine the value of the interaction of genotype and environment for three important agronomic traits (seed yield, protein content and oil content) using anadditive main effects and multiplicative interaction (AMMI) statistical model, and to identify stable sources for breeding for listed traits. The results of the research indicated that all traits were strongly influenced by environmental factors, while the influence of genotype and particularly interaction of genotype and environment was of less importance. AMMI analysis enabled identification of genotypes with above average value and high stability for seed yield (three genotypes), protein content (three genotypes) and oil content (two genotypes), which could be utilized as potential stable sources of variability in future soybean breeding programs.
Keywords: AMMI analysis, soybean, seed yield, seed composition
Corresponding author: Vesna Perić, Maize Research Institute “Zemun Polje”, Slobodana Bajića 1, 11000 Belgrade, Serbia. Phone + 381 11 3756704. e-mail: vperic@mrizp.rs
Published in „GENETIKA“ Vol. 53, No. 1(2021), pp. 339-348
© 2021 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.
https://doi.org/10.2298/GENSR2101339A
Original scientific paper
MATRIX METALLOPROTEINASE 7 (MMP-7) AND TISSUE INHIBITOR OF METALLOPROTEINASES-2 (TIMP-2) DOWNREGULATION COMPLEMENTS PLAG1 ONCOGENE OVEREXPRESSION IN PLEOMORPHIC ADENOMA PATHOGENESIS
Boban ANICIC1, Nadja NIKOLIC2, Jelena CARKIC2, Drago JELOVAC1, Zoran JEZDIC1, Branko DOZIC3, Vesna DANILOVIC4, Jelena MILASIN2
1University of Belgrade, School of Dental Medicine, Clinic for Maxillofacial Surgery, Belgrade, Serbia
2University of Belgrade, School of Dental Medicine, Department of Human Genetics, Belgrade, Serbia
3University of Belgrade, School of Dental Medicine, Department of Pathology, Belgrade, Serbia
4University of Belgrade, School of Dental Medicine, Department of Histology, Belgrade, Serbia
Abstract
Anicic B., N. Nikolic, J. Carkic, D. Jelovac, Z. Jezdic, B. Dozic, V. Danilovic, J. Milasin (2021). Matrix metalloproteinase 7 (MMP-7) and tissue inhibitor of metalloproteinases-2 (TIMP-2) downregulation complements plag1 oncogene overexpression in pleomorphic adenoma pathogenesis.- Genetika, Vol 53, No.1, 339-348.
Pleomorphic adenoma (PA) is the most common neoplasm of salivary glands and consists of epithelial and mesenchymal components. Although a benign lesion, it harbors a potential for recurrence and malignant transformation. Also, due to its histological diversity and unpredictive behavior PA can represent both diagnostic and therapeutic challenge. Matrix metalloproteinases (MMPs) are well known modifiers of extracellular matrix (ECM) PA component and in conjunction with their endogenous tissue inhibitors (TIMPs) may influence PA tumor biology. PLAG1 oncogene also has an important role in PA; however, neither the exact mechanisms of its influence nor its interactions with other genes are completely elucidated. The aims of this study were to assess the expression of PLAG1, MMP-2, MMP-7, MMP-9, TIMP-1 and TIMP-2 genes in PAs, and find a possible association of gene expression levels with clinical/epidemiological parameters of PA patients. Relative mRNA levels were assessed using Quantitative real-time PCR analyses in 15 PAs of the parotid gland and 5 normal salivary glands (NSGs). A statistically significant overexpression of PLAG1 was observed in PA compared to NSG samples (P=0.010); PA had 5.48 times higher mRNA levels than NSG. Out of the three analyzed MMP genes, significantly lower levels of MMP-7 were found in PA patients (P=0.026). TIMP2 was also downregulated in PA samples, compared to NSGs (P=0.040). MMP-7 and TIMP-2 mRNA levels were decreased 2.95 and 2.85 times respectively, in PA samples. No association was found between gene expression and clinical/epidemiological PA parameters. Our results suggest that PLAG1 overexpression with concomitant MMP7 and TIMP2 downregulation may contribute to PA development.
Keywords: salivary gland, pleomorphic adenoma, MMP-7, TIMP-2, PLAG1, gene expression
Corresponding author: Dr Jelena Milašin, University of Belgrade, School of Dental Medicine, Department of Human Genetics, Dr Subotica 8, 11000 Belgrade, Serbia, phone: + 38111 2685288, fax: +38111 2685361, e-mail: jelena.milasin@stomf.bg.ac.rs
Published in „GENETIKA“ Vol. 53, No.1(2021), pp. 349-362
© 2021 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
https://doi.org/10.2298/GENSR2101349P
Original scientific paper
RAPD PROFILING IN DETECTING GENETIC VARIATION
IN Stellaria L. (Caryophyllaceae)
Xiaobang PENG 1*, Majid KHAYYATNEZHAD2 and Leila JOUDI GHEZELJEHMEIDAN3*
1Department of Biological and Medical Engineering, ShangLuo University, Shaanxi Shangluo, 726000, China
2Young Researchers Club, Ardabil Branch, Islamic Azad University, Ardabil, Iran
3Department of Agriculture, Shabestar Branch, Islamic Azad University, Shabestar, Iran
Abstract
Peng X., M. Khayyatnezhad and L. Joudi Ghezeljehmeidan (2021). RAPD profiling in detecting genetic variation in Stellaria L. (Caryophyllaceae).- Genetika, Vol 53, No.1,349 -362.
Stellaria species are common herbs, preferred humid mountainously slopes, but some grew in desert. Main center of diversification for Stellaria is Eurasia, with a center of distribution in the mountains of central Asia. Some species are also cosmopolitan. It is represented by 9 species in Iran. The genus has high medicinal value. To determine the genetic diversity and understand the species’ limits within the Iranian Stellaria, we produced molecular data using 139 randomly collected plants representing 8 species from five provinces of Iran. A total of 122 reproducible bands were generated by 10 of 25 random amplified polymorphic DNA (RAPD) primers, with an average of 12.2 bands/primer and 33% polymorphism. Largest number of effective alleles (Ne), genetic diversity (H), and Shannon Index (I) were shown by S. media. Our data depicted highest similarity between S. media and S. pallida and lowest between S. media and S. graminea. S. pallida showed relatively low level of genetic variation. Finally, the Neighbor Joining (NJ) trees based on RAPD markers data divided the populations into two different clusters, indicating their genetic difference which is discussed in details.
Keywords: Endemism, Gene flow, Random Amplified Polymorphic DNA (RAPD).
Corresponding author: Xiaobang Peng, Department of Biological and Medical Engineering, ShangLuo University, Shaanxi Shangluo, 726000, China, Email: penigxiaobang@126.com;
Leila Joudi Ghezeljehmeidan, Department of Agriculture, Shabestar Branch, Islamic Azad University, Shabestar, Iran, Joudi.leila@yahoo.com
Published in „GENETIKA“ Vol. 53 No. 1(2021), pp. 363-378
© 2021 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
https://doi.org/10.2298/GENSR2101363Y
Original scientific paper
EVALUATION OF GENETIC DIVERSITY IN Geranium (Geraniaceae)
USING RAPD MARKER
Juan YIN1*, Majid KHAYATNEZHAD2, Abdul SHAKOOR3,4
1Forestry College, Xinyang College of Agriculture and Forestry, Xinyang, Henan, 464000, China
2Department of Environmental Sciences and Engineering, Ardabil Branch, Islamic Azad University, Ardabil, Iran
3College of Environment and Planning, Henan University, Kaifeng, 475004, Henan, China
4Key Laboratory of Geospatial Technology for the Middle and Lower Yellow River Regions, Ministry of Education, Kaifeng 475004, Henan, China
Abstract
Yin J., M. Khayatnezhad, A. Shakoor (2021). Evaluation of genetic diversity in geranium (Geraniaceae) using RAPD marker.- Genetika, Vol 53, No.1,363 -378.
Genetic diversity studies are essential to understand the conservation and management of plant resources in any environment. No detailed Random Amplified Polymorphic DNA (RAPD) studies were conducted to study Geranium genetic diversity. Therefore, we collected and analyzed thirteen species from nine provinces. Overall, one hundred and twenty-five plant specimens were collected. Our aims were 1) to assess genetic diversity among Geranium species 2) is there a correlation between species genetic and geographical distance? 3) Genetic structure of populations and taxa. We showed significant differences in quantitative morphological characters in plant species. Unweighted pair group method with arithmetic mean and multidimensional scaling divided Geranium species into two groups. G. sylvaticum depicted unbiased expected heterozygosity (UHe) in the range of 0.11. Shannon information was high (0.38) in G. columbinum. G. sylvaticum showed the lowest value, 0.14. The observed number of alleles (Na) ranged from 0.25 to 0.55 in G. persicum and G. tuberosum. The effective number of alleles (Ne) was in the range of 1.020-1.430 for G. tuberosum and G. collinum. Gene flow (Nm) was relatively low (0.33) in Geranium. The Mantel test showed correlation (r = 0.27, p=0.0002) between genetic and geographical distances. We reported high genetic diversity, which clearly shows the Geranium species can adapt to changing environments since high genetic diversity is linked to species adaptability. Present results highlighted the utility of RAPD markers and morphometry methods to investigate genetic diversity in Geranium species.
Keywords: Gene flow, Random Amplified Polymorphic DNA (RAPD), Geranium, isolation, morphometry.
Corresponding author: Juan Yin, Forestry College, Xinyang College of Agriculture and Forestry, Xinyang, Henan, China. E-mail: yinjuan20210313@163.com, ldongmei870@gmail.com
Published in „GENETIKA“ Vol. 53, No.1(2021), pp. 379-391
© 2021Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
https://doi.org/10.2298/GENSR2101379S
Original scientific paper
EVALUATION OF DIFFERENT GROWTH HORMONES MEDIATED CALLUS INDUCTION AND REGENERATION AS WELL AS THE EFFECT OF COLCHICINES, ETHYL METHANESULFONATE (EMS) AND GAMMA RADIATION ON SOME TRAITS OF Impatiens walleriana
Sedigheh Sadat SHARAFI1, Pejman AZADI2, Maryam JAFARKHANI KERMANI3,
Vahid ABDOSSI1, Ali ESKANDRI4
1Department of Horticultural Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 Department of Genetic Engineering, Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj, Iran
3 Department of Tissue and Cell Culture, Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj, Iran
4Department of Nuclear Agriculture, Karaj, Iran
Abstract
Sharafi S. S., P. Azadi, M. Jafarkhani Kermani, V. Abdossi, A. Eskandri (2021). Evaluation of different growth hormones mediated callus induction and regeneration as well as the effect of colchicines, ethyl methanesulfonate (EMS) and gamma radiation on some traits of Impatiens walleriana. - Genetika, Vol 53, No.1, 379-391.
Impatiens is an ornamental member of family Balsaminaceae. This plant mostly propagated by vegetative technique, which generally time wasting process. It is often multiplicities via seed but is barricaded by F1 seed sterility. In vitro culture of Impatiens walleriana has much significant function in fast proliferation with useful features and elicitation of healthful and disease-free plants. This experiment was conducted to investigate the impact of medium and different hormones on in vitro propagation of Impatiens walleriana by using a completely randomized design. MS medium was prepared along with various concentrations of BAP, TDZ and ZEA. Callus was induced and grew well in media supplemented with 0.5 mg/l NAA + 1 mg/l BAP. In order to indirect propagation, explants were cultured in same media containing BAP, ZEA and TDZ in combination with NAA. These treatments have ability to organogenesis. The results revealed that the control treatment had the lowest effect on traits including shoot percentage, number of shoots, number of leaves, shoot length, fresh and dry weight, and it lead to maximum proliferations in medium supplemented with 0.5 mg/l NAA + 1 mg/l BAP. The highest root length and rooting percentage was observed in 0.5 mg/l IBA + 0.5 mg/l BAP. In addition, the effect of mutation agents was studied. Aseptic samples were treated with γ- irradiation, Ethyl Methane Sulfonate and colchicines at growth chamber. Treatments with 30 and 60 grey respectively had the lower survival rate, growth rate and polyploidy while colchicines with 0.1 and 0.2 had the highest rats. Regarding to these, the present technique illustrate an effective system for in vitro reproduction of Impatiens walleriana by hypocotyls cultures. In addition, colchicines proved to be effective in induction of polyploidy in this plantlet.
Keywords: Colchicines, Ethyl methanesulfonate, Gamma radiation, Impatiens walleriana, Tissue culture
Corresponding author: Pejman Azadi, Department of Genetic Engineering, Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj, Iran
Published in „GENETIKA“Vol. 53, No.1(2021), pp. 393-405
© 2021 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
https://doi.org/10.2298/GENSR2101393B
Original scientific paper
MOLECULAR IDENTIFICATION AND GENETIC DIVERSITY IN Hypericum L.:
A HIGH VALUE MEDICINAL PLANT USING RAPD MARKERS MARKERS
Dezhong BI1,*, Dan CHEN2, Majid KHAYATNEZHAD3, Zohreh sayyah HASHJIN4, Zifa LI2*, Yuexiang MA1
1School of Chinese Medicine, Shandong University of Traditional Chinese Medicine, Jinan, China
2Experimental Centre, Shandong University of Traditional Chinese Medicine, Jinan, China
3Department of Environmental Sciences and Engineering, Ardabil Branch, Islamic Azad University, Ardabil, Iran
4Department of Horticultural Science, Faculty of agriculture, University of Tabriz, Tabriz, Iran
*Equal contribution
Abstract
Bi D., D. Chen, M. Khayatnezhad, Z. S. Hashjin, Z. Li, Y. Ma (2021). Genetic response of growth phases for abiotic environmental stress tolerance in cereal crop plants. - Genetika, Vol 53, No.1,393-405.
Genus Hypericum (Guttiferae, Hypericoideae) is perennial, belonging to the Hypericaceae family, having 484 species in forms of trees, shrubs, and herbs, distributed in 36 taxonomic sections. No detailed Random Amplified Polymorphic DNA (RAPD) studies were conducted to study Hypericum genetic diversity. Therefore, we collected and analyzed six species from five provinces of Iran regions. Overall, seventy plant specimens were collected. Our aims were 1) to assess genetic diversity among Hypericum species 2) is there a correlation between species genetic and geographical distance? 3) Genetic structure of populations and taxa. We showed significant differences in quantitative morphological characters in plant species. H. dogonbadanicum depicted unbiased expected heterozygosity (UHe) in the range of 0.10. Shannon information was high (0.32) in H. perforaturm. H. dogonbadanicum showed the lowest value, 0.17. The observed number of alleles (Na) ranged from 0.22 to 0.53 in H. dogonbadanicum and H. elongaturn. Gene flow (Nm) was relatively low (0.87) in Hypericum. The Mantel test showed correlation (r = 0.45, p=0.0001) between genetic and geographical distances. We reported high genetic diversity, which clearly shows the Hypericum species can adapt to changing environments since high genetic diversity is linked to species adaptability. Present results highlighted the utility of RAPD markers and morphometry methods to investigate genetic diversity in Hypericum species.
Keywords: Gene flow, Hypericum, Random Amplified Polymorphic DNA (RAPD), morphometry
Corresponding author: Yuexiang Ma, School of Chinese Medicine, Shandong University of Traditional Chinese Medicine, No. 4655 University Road, University Science Park, Changqing District, Jinan City, Shandong Province, 250355, P.R.China; E-mail: myx1008@126.com;
Zifa Li, Experimental Centre, Shandong University of Traditional Chinese Medicine, No. 4655 University Road, University Science Park, Changqing District, Jinan City, Shandong Province, 250355,P.R.China, E-mail: zifa_0611@163.com; majidkhayatnezhad126@gmail.com
Published in „GENETIKA“Vol. 53, No.1(2021), pp. 407-417
© 2021 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.633.11
https://doi.org/10.2298/GENSR2101407V
Original scientific paper
FROM NEOLITHIC TO LATE MODERN PERIOD: BRIEF HISTORY OF WHEAT
Ana VELIMIROVIC1, Zoran JOVOVIC1, Novo PRŽULJ2
1University of Montenegro, Biotechnical Faculty, Podgorica, Montenegro
2University of Banja Luka, Agricultural Faculty, The Republic of Srpska, Bosnia and Herzegovina
Abstract
Velimirovic A., Z. Jovovic, N. Pržulj (2021). From Neolithic to late modern period: brief history of wheat.- Genetika, Vol 53, No.1,407-417.
History of wheat cultivation is as long as history of civilization. Adaptation of nature, animal domestication and plant cultivation, enabled transition from nomadism to sedentism 12,000 years ago, portraying the rise of Homo sapiens of today. First civilization, Mesopotamia aroused around 4000 B.C.E, in the river banks of Tiger and Euphrates, where carbon-14 dating revealed that tetraploid wild emmer (Triticum turgidum subsp. dicoccoides) was grown. Due to modest cultivation requirements and high nutritional value, wheat quickly spread from its centre of origin throughout the world. Generations of farmers have chosen seeds from plants with best architecture, adapted to local conditions for sowing, striving toward constant improvement of yields. For centuries agricultural production was based on locally adapted wheat varieties of great genetic diversity. Agriculture completely changed its course in mid-XX century as a result of Green Revolution, introduction of high-yielding cereal varieties, chemical fertilizers and pesticides, irrigation and mechanization replacing traditional techniques. The flourishing of agriculture has drastically changed the course of agricultural development and global society. Improvement of agricultural techniques by integrating scientific advancements and knowledge to assimilate environmental factors has tripled wheat yields in last 50 years. Today, wheat, maize and rice, represent staple food for humanity.
Keywords: wheat, agricultural evolution, green revolution, wheat cultivation
Corresponding author: Ana Velimirovic, University of Montenegro, Biotechnical Faculty, Mihaila Lalića 15, Podgorica, Montenegro, E-mail: ana.velimirovic@hotmail.com, phone: 00382 69 895 805
Published in „GENETIKA“Vol. 53, No.1(2021), pp.419-456
© 2021 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.633.
https://doi.org/10.2298/GENSR2101419A
Original scientific paper
GENETIC RESPONSE OF GROWTH PHASES FOR ABIOTIC ENVIRONMENTAL STRESS TOLERANCE IN CEREAL CROP PLANTS
Qurban ALI* and Arif MALIK
Institute of Molecular Biology and Biotechnology, University of Lahore, Lahore Pakistan
Abstract
Ali Q. and A. Malik (2021). Genetic response of growth phases for abiotic environmental stress tolerance in cereal crop plants. - Genetika, Vol 53, No.1,419-456.
The yield potential and quality of main cereals crop plants including maize, wheat, rice and barley have improved through breeding and introduction of transgenic crop plants from last three decades. There has been intensive research for the improvement of resistance against biotic and abiotic environmental conditions to safe the potential of cereal crop plants. Among abiotic stresses drought and heat are two most important abiotic factors which caused major loss in yield and quality of crop plants. The heat stress leads towards drought due to loss of water from soil and plant surfaces, therefore drought and heat caused combined adverse effects on plant morphological, physiological and yield traits which leads to reduce crop plant potential. There has been always an interaction among the environmental conditions and crop plants to produce grain and restore productivity. The drought and heat stress caused changes at cellular level, molecular changes and gene expression changes in cereals at various vegetative and reproductive stages/phases of crop growth and development. A large number of genes have indentified in cereals which switch up-regulated and down-regulated during drought and heat stress conditions. However, there is a need to improve resistance in cereals at gene level to maintain potential of yield and quality under abiotic stress conditions like drought, heat, salinity, and cold.
Keywords: drought, heat, maize, wheat, rice, reproductive phase, plant and environmental interactions
Corresponding author: Qurban Ali, Institute of Molecular Biology and Biotechnology, University of Lahore, Lahore Pakistan, E-mail: saim1692@gmail.com