GENETIKA, Vol

GENETIKA, Vol. 48, No.3 (2016)

Reza DARVISHZADEH

DETECTION OF ISSR MARKERS LINKED TO SEED OIL BIOCHEMICAL CHARACTERISTICS IN CASTOR (Ricinus communis L.) THROUGH ASSOCIATION ANALYSIS [Abstract ] [Full text ]

Sajjad ghorghanlu, Mohammad asgharzadeh, Hossein samadi-kafil, Fatemeh khaki-khatibi, Jalil rashedi, Behroz mahdavi poor, Seyyed Reza moaddab, Mahya pourostadi

TNF-Α -308 G/A POLYMORPHISM AND SUSCEPTIBILITY TO TUBERCULOSIS IN AZERI POPULATION OF IRAN [Abstract ] [Full text ]

Nada hladni, Siniša jocić, Anto mijić, Vladimir miklič, Dragana miladinović

CORRELATION AND PATH ANALYSIS of yield and yield components of confectionary sunflower [Abstract ] [Full text ]

Gashtasb mardani, Amir Hossein mahvi, Morteza hashemzadeh-chaleshtori, Simin nasseri, Mohammad Hadi dehghani, Payam ghasemi-dehkordi

DEGRADATION OF PHENANTHRENE AND PYRENE USING GENETICALLY ENGINEERED DIOXYGENASE PRODUCING Pseudomonas putida IN SOIL [Abstract ] [Full text ]

Karina cesaityte and Danielius serapinas

THE SPECTRUM OF MICRODELETIAN SYNDROMES AT THE HOSPITAL OF LITHUANIAN UNIVERSITY OF HEALTH SCIENCES [Abstract ] [Full text ]

Gordana branković, Vesna dragičević, Slađana žilić, Desimir knežević, Nenad đurić, Dejan dodig

EXPECTED GENETIC ADVANCE AND STABILITY OF PHYTIC ACID AND ANTIOXIDANTS CONTENT IN BREAD AND DURUM WHEAT [Abstract ] [Full text ] [Supplement]

Chandrawati, Neha SINGH, Vrijesh Kumar YADAV, Rajendra KUMAR, Sujit KUMAR and Hemant Kumar YADAV

GENETIC VARIABILITY AND INTERRELATIONSHIP AMONG MORPHOLOGICAL AND YIELD TRAITS IN LINSEED (Linum usitatissimum L.) [Abstract ] [Full text ]

Elham kazemi and Danial kahrizi

LACK OF ASSOCIATION BETWEEN GASTRIC CANCER AND HOPQ ALLELES IN Helicobacter pylori [Abstract] [Full text]

Jovanka lukic, Miroslav dinic, Mirjana marcetic, Marina milenkovic, Jelena begovic, Ivana strahinic

EFFECTS OF SOYBEAN CARBOHYDRATES AND Lactobacillus helveticus BGRA43 ON METABOLIC PROCESSES IN RAT COLON [Abstract ] [Full text ]

Omer beyhan, Ecevit eyduran, Meleksen akın, Sezai ercisli, Mustafa Kenan gecer, Ahmet Erhan karahan

IDENTIFICATION OF THE RELATIONSHIP BETWEEN SOME CHARACTERISTICS OF NATIVE WALNUT GENOTYPES PECULIAR TO DARENDE DISTRICT OF MALATYA PROVINCE: USE OF FACTOR ANALYSIS SCORES IN MULTIPLE LINEAR REGRESSION [Abstract ] [Full text ]

Dušica S. ademović-sazdanić, Svetlana I. vojvodić,Stevan L. popović and Nada V. konstantinidis

STUDY OF KILLER CELL IMMUNOGLOBULIN-LIKE RECEPTOR GENES AND HLA C LIGANDS IN HEMATOPOIETIC STEM CELL TRANSPLANTATION PAIRS IN VOJVODINA [Abstract ] [Full text ]

Dana gabriková, Daniela grejtáková, Jarmila bernasovská, Anna pavúková, Barbora lewandowská, Mariana bindasová, Janakisková, Ivana klobušovská, DanicaHronská, Soňa mačeková

SEQUENCE ANALYSIS OF CANDIDATE GENES IN TWO ROMA FAMILIES WITH SEVERE TOOTH AGENESIS [Abstract ] [Full text]

Bojana PETROVIĆ, AND Jelena DUKANAC-STAMENKOVIĆ

CONFINED PLACENTAL MOSAICISM IN SHORT TERM CULTURE [Abstract ] [Full text ]

A. K. singh, Sanjay kumar and Neha singh

DETECTING LEVEL OF GENETIC DIFFERENTIATION IN TWO CLOSELY RELATED SPECIES OF Drosophila: D. bipectinata AND D. malerkotliana [Abstract ] [Full text ]

Antonela markulj kulundžić, Marija viljevac vuletić, Siniša jocić, Sandra cvejić, Maja matoša kočar, Anto mijić, Ivica liović, Aleksandra sudarić, Hrvoje lepeduš, Josip kovačević, Ana josipović

EFFECT OF DIFFERENT SOIL WATER CONTENT EFFECT ON GENOTYPE EXPESSION IN PHOTOSYNTHETIC EFFICIENCY AND LEAF TEMPERATURE IN SUNFLOWER [Abstract ] [Full text ]

Igor balalić, Jovan crnobarac, Siniša jocić, Vladimir miklič, Velimir radić, Nenad dušanić

VARIABILITY OF HEAD DIAMETER IN SUNFLOWER HYBRIDS DEPENDING ON PLANTING DATE [Abstract] [Full text ]

Dane boshev, Mirjana jankulovska, Sonja ivanovska, Ljupcho jankuloski

ASSESMENT OF WINTER WHEAT ADVANCED LINES BY USE OF MULTIVARIATE STATISTICAL ANALYSES [Abstract ] [Full text ]

Maja ječmenica, Natalija kravić, Mirjana vasić, Tomislav živanović, Violeta mandić, Jelena damnjanović, Vesna dragičević

GENETIC VARIABILITY OF FREE ENERGY IN A FUNCTION OF DROUGHT TOLERANCE IN COMMON BEAN ACCESSIONS [Abstract ] [Full text ]

Hulya unver, Ebru sakar, Mehmet ulas, Sezai ercisli⁴, Bekir Erol ak

MOLECULAR CHARACTERIZATION OF INDIGENOUS OLIVE GENOTYPES BASED ON SSR ANALYSIS [Abstract ] [Full text ]

Milka BRDAR-JOKANOVIĆ, Zdenka GIREK, Milan UGRINOVIĆ, Vladimir SIKORA, Ivica ĐALOVIĆ, Jasmina ZDRAVKOVIĆ

AMMI MODEL IN THE ANALYSIS OF GENOTYPE BY ENVIRONMENT INTERACTION OF CONVENTIONALLY AND ORGANICALLY GROWN ONION [Abstract ] [Full text]

Marija tanasković, Zorana kurbalija novičić, Bojan kenig, Marija savić veselinović, Marina stamenković-radak, Marko anđelković

SYNERGISTIC EFFECT OF ENVIRONMENTAL AND GENOMIC STRESS ON WING SIZE OF Drosophila subobscura [Abstract ] [Full text]

Sekip erdal, Mehmet pamukcu, Ahmet ozturk, Koksal aydinsakir, Ozlemyilmaz dogu

MORPHO-PHYSIOLOGICAL COMBINING ABILITY AMONG TROPICAL AND TEMPERATE MAIZE GERMPLASM FOR DROUGHT TOLERANCE [Abstract ] [Full text]

Ana nikolić, Jelena bogosavljević, Zoran čamdžija, Milomir filipović, Dragan kovačević, Milan STEVANOVIĆ, Snežana mladenović drinić

ESTABLISHMENT AND CONFIRMATION OF HETEROTIC GROUPS AND GENETIC DIVERSITY ASSESSMENT OF MAIZE INBRED LINES USING MICROSATELLITE DATA [Abstract ] [Full text]

Ana marjanović jeromela, Aleksandra dimitrijević, Sreten terzić, Aleksandar mikić, Jovanka atlagic, Dragana miladinović, Mirjana jankulovska, Jasna savić, Wolfgang friedt

APPLYING MENDELIAN RULES IN RAPESEED (Brassica napus) BREEDING [Abstract ] [Full text]

Aram ARSHADI, Ezzat KARAMI, Behzad KHATERI, Payam REZABAKHSH

DROUGHT STRESS EFFECTS ON THE GRAIN YIELD AMONG DIFFERENT BARLEY CULTIVARS [Abstract ] [Full text]

Ahmad Reza GOLPARVAR, Amin HADIPANAH

DIVERSITY IN CHEMICAL COMPOSITION AND YIELD OF ESSENTIAL OIL FROM TWO MENTHA SPECIES [Abstract ] [Full text]

Wang NA, Wang HONG, Hui-Ying LIU, Muhammad IRFAN, Shi-Wei NIU, Yang TAO

DIVERSITY OF METHANE-PRODUCING MICROBIAL COMMUNITY STRUCTURE WITH 16S-V3 rDNA PCR-DGGE [Abstract ] [Full text]

Published in „GENETIKA“ Vol. 48, No. 3 (2016), pp. 807-817

UDC 575.630

DOI: 10.2298/GENSR1603807D

Original scientific paper

DETECTION OF ISSR MARKERS LINKED TO SEED OIL BIOCHEMICAL CHARACTERISTICS IN CASTOR (Ricinus communis L.) THROUGH ASSOCIATION ANALYSIS

Reza DARVISHZADEH^1,2

^{^[1]}Department of Plant Breeding and Biotechnology, Urmia University, Urmia, Iran

²Institue of Biotechnology, Urmia University, Urmia, Iran

Abstract

Darvishzadeh R. (2016): Detection of ISSR markers linked to seed oil biochemical characteristics in castor (Ricinus communis L.) through association analysis.- Genetika, Vol 48, No.3, 807 -817.

In major crops, panels of germplasm accessions have been assembled for allele mining at targeted candidate genes and for whole-genome association mapping studies. A germplasm collection of 60 genotypes belong to 12 castor accessions from six different provinces of Iran were evaluated for seven biochemical traits as important seed oil components and tested for significant association with the allelic profile of 16 ISSR primers. Study of population structure as a prerequisite for association analysis showed that there is six possible subgroups (K=6) in the studied population that also barplot results confirmed it. Mixed linear model (MLM) were used to discover marker/trait associations. As a result, 37 marker-trait significant associations (P≤0.05) were identified and a maximum number of marker-trait associations (27.03%) were identified for Stearic acid. The identified and associated markers are expected to be useful in marker-aided breeding of high oil content genotypes in castor.

Keywords: association mapping, castor, linkage disequilibrium, mixed linear model, molecular markers.

Corresponding author: Dr. R. Darvishzadeh, Department of Plant Breeding and Biotechnology, Urmia University, Urmia, Iran. E-mail: r.darvishzadeh@urmia.ac.ir. Fax: +98 44 32779558.

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Published in „GENETIKA“ Vol. 48, No. 3 (2016), pp. 819 - 826

Serbia

UDC 575

DOI: 10.2298/GENSR1603819G

Original scientific paper

TNF-Α -308 G/A POLYMORPHISM AND SUSCEPTIBILITY TO TUBERCULOSIS

IN AZERI POPULATION OF IRAN

Sajjad ghorghanlu¹, Mohammad asgharzadeh^2’*, Hossein samadi-kafil³, Fatemeh khaki-khatibi⁴, Jalil rashedi², Behroz mahdavi poor², Seyyed Reza moaddab², Mahya pourostadi⁵

¹Biotechnology Research Center, Faculty of Advanced Medical Sciences,

Tabriz University of Medical Sciences, Tabriz, Iran

² Biotechnology Research Center, Paramedical Faculty, Tabriz University of Medical Sciences, Tabriz, Iran

³Drug Applied Research Center, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

⁴Department of Clinical Biochemistry, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

⁵ Hematology Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

Abstract

ghorghanlu S., M. asgharzadeh, H. samadi-kafil, F. khaki-khatibi, J. rashedi, B. mahdavi poor, S. Reza moaddab, M. pourostadi (2016): TNF-Α -308 G/A polymorphism and susceptibility to tuberculosis in Azeri population of Iran. - Genetika, Vol 48, No.3, 819 -826.

Single nucleotide polymorphisms (SNPs) in cytokine genes may alter the level and function of secreted cytokine; therefore, SNPs can influence the immune response. The aim of the present study was to determine the association of TNF-α -308G/A single nucleotide polymorphism in tuberculosis patients in the Azeri population of Iran. The TNF-308G/A single nucleotide polymorphism in the promoter region was genotyped by using the allele-specific PCR method in 200 healthy controls and 124 tuberculosis patients.

The distribution of allele frequencies for TNF-α -308G/A polymorphism between control and tuberculosis patient groups was not significant (P-value = 0.058, OR = 1.5). Furthermore, no statistically significant association was found between TNF-α -308G/A genotype and resistance/susceptibility to TB (P-value = 0.102). Our results suggest that TNF-α -308G/A polymorphism has no measurable effect on the development of tuberculosis in Azeri population of Iran.

Keywords: association, single nucleotide polymorphism, tuberculosis, tumor necrosis factor-α, susceptibility

Corresponding author: Mohammad Asgharzadeh, Paramedical Faculty, Tabriz University of Medical Sciences, Tabriz, Iran; Tel/fax: +984133371971; E–mail: Asgharzadehmo@yahoo.com

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Published in „GENETIKA“ Vol. 48, No. 3 (2016), pp. 827- 835

Serbia

UDC 575.630

DOI: 10.2298/GENSR1603827H

Original scientific paper

CORRELATION AND PATH ANALYSIS of yield and yield components

of confectionary sunflower

Nada hladni¹, Siniša jocić¹, Anto mijić², Vladimir miklič¹, Dragana miladinović¹

¹Institute of Field and Vegetable Crops, Novi Sad, Serbia

²Agricultural Institute Osijek, Osijek, Croatia

Abstract

Hladni N., S. Jocić, A. Mijić, V. Miklič, D. Miladinović (2016): Correlation and path analysis of yield and yield components of confectionary sunflower. - Genetika, Vol 48, No.3, 827 - 835.

Path coefficient analysis was applied to gain information on direct and indirect effects of studied traits (seed oil content, kernel oil content, seed protein content, kernel protein content, head diameter, kernel ratio and plant height) on sunflower seed yield. Traits which exhibit the highest effects on seed yield will be used as a selection criterion in confectionary sunflower breeding. The research was conducted during three vegetation seasons on 22 experimental confectionary sunflower hybrids, created in breeding program the Institute of Field and Vegetable Crops. Among the largest number of examined traits, significant and highly significant correlations were found. With the analysis of simple correlation coefficients a low interdependence was determined between, kernel protein content, plant heights with seed yield. Negative but weak correlation was determined between kernel oil content, seed protein content, kernel ratio with seed yield. Established a negative strong correlation of head diameter (-0.190^*) and negative very strong correlation of seed oil content (-0.351^**) with seed yield. The seed oil content had a very strong direct negative effect on seed yield (DE=-0.831^**). The head diameter, plant height, seed protein content had weak negative direct effect on seed yield. Kernel protein content and kernel oil content has demonstrated a weak direct positive effect on seed yield. Path coefficient analysis for seed yield showed very strong direct effect for kernel ratio (DE=0.487^*) on seed yield, it shows that the kernel ratio is important selection criterion for confectionary sunflower breeding.

Keywords: confectionary sunflower, seed yield, yield components, correlations, path coefficient analysis

Corresponding author: Nada Hladni, Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad, Serbia, Phone: 381 21 780 365, Fax: 381 21 780 198, E-mail: nada.hladni@ifvcns.ns.ac.rs

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Published in „GENETIKA“ Vol. 48, No3 (2016), pp. 837- 858

Serbia

UDC 575.630

DOI: 10.2298/GENSR1603837M

Original scientific paper

DEGRADATION OF PHENANTHRENE AND PYRENE USING GENETICALLY ENGINEERED DIOXYGENASE PRODUCING Pseudomonas putida IN SOIL

Gashtasb mardani¹, Amir Hossein mahvi^1,2*,

Morteza hashemzadeh-chaleshtori⁴, Simin nasseri^1,3,

Mohammad Hadi dehghani¹, Payam ghasemi-dehkordi⁴

¹Department of Environmental Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

² Center for Solid Waste Research, Institute for Environmental Research, Tehran University of Medical Sciences, Tehran, Iran

³Center for Water Quality Research (CWQR), Institute for Environmental Research (IER), Tehran University of Medical Sciences, Tehran, Iran

⁴ Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran

Abstract

mardani G., A. H. mahvi, M. hashemzadeh-chaleshtori, S. naseri, M.H. dehghani, P. ghasemi-dehkordi (2016): Degradation of phenanthrene and pyrene using genetically engineered dioxygenase producing Pseudomonas putida in soil. - Genetika, Vol 48, No.3, 837 - 858.

The aim of the present study was application of genetically engineered P. putida that produce dioxygenase for degradation of phenanthrene and pyrene in spiked soil using high-performance liquid chromatography (HPLC) method. The nahH gene that encoded catechol 2,3-dioxygenase (C23O) was cloned into pUC18 and pUC18-nahH recombinant vector was generated and transformed into wild P. putida, successfully. The genetically modified and wild types of P. putida were inoculated in soil and pilot plan was prepared. Finally, degradation of phenanthrene and pyrene by this bacterium in spiked soil were evaluated using HPLC measurement technique. The results were showed elimination of these PAH compounds in spiked soil by engineered P. putida comparing to dishes containing natural soil with normal microbial flora and inoculated autoclaved soil by wild type of P. putida were statistically significant (p<0.05). Although adding N and P chemical nutrients on degradation ability of phenanthrene and pyrene by engineered P. putida in soil were not statistically significant (p>0.05) but it was few impact on this process (more than 2%). Additional and verification tests including catalase, oxidase and PCR on isolated bacteria from spiked soil were indicated that engineered P. putida was alive and functional as well as it can affect on phenanthrene and pyrene degradation via nahH gene producing. These findings indicated that genetically engineered P. putida generated in this work via producing C23O enzyme can useful and practical for biodegradation of phenanthrene and pyrene as well as petroleum compounds in polluted environments.

Keywords: catechol 2,3-dioxygenase, HPLC, phenanthrene, pyrene, Pseudomonas putida

Corresponding author: Amir Hossein Mahvi, Department of Environmental Health Engineering, School of Public Health and Center for Solid Waste Research, Institute for Environmental Research, Tehran University of Medical Sciences, Tehran, Iran, E-mail: biotechnologyskums@gmail.com, ahmahvi@yahoo.com,Tell: +98-38-33346692. Fax: +98-38-33330709

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Published in „GENETIKA“ Vol. 48, No.3 (2016), pp. 859 - 866

Serbia

UDC 575

DOI: 10.2298/GENSR1603859C

Original scientific paper

THE SPECTRUM OF MICRODELETIAN SYNDROMES AT THE HOSPITAL OF LITHUANIAN UNIVERSITY OF HEALTH SCIENCES

Karina cesaityte¹and Danielius serapinas^1,2

¹Department of Genetics and molecular medicine, Medical Academy,

Lithuanian University of Health Sciences, Kaunas, Lithuania

²Mykolas Romeris University, Vilnius, Lithuania

Abstract

cesaityte K. and D. serapinas (2016): The spectrum of microdeletian syndromes at the hospital of Lithuanian University of Health Sciences. - Genetika, Vol 48, No.3, 859-866.

Microdeletion syndrome is a rare condition which can be diagnosed by fluorescent in situ hybridization (FISH) method. We analyzed microdeletion syndromes cases during ten years period (2005-2015) at The Hospital of Lithuanian University of Health Sciences. We report 2 patients with Prader-Willi syndrome, 2 patients with Smith-Magenis syndrome, 1 patient with Angelman syndrome and 1 patient with Cri du Chat syndrome. All syndromes were confirmed by FISH. These cases contain mainly data about phenotype abnormalities and clinical symptoms.

Keywords: microdeletion syndrome, Fluorescence in situ hybridization (FISH), phenotype

Corresponding author: Danielius Serapinas, Lithuanian University of Health Sciences, Medical Academy, Department of Genetics and molecular medicine Eivenių 2, Kaunas LT50009, Lithuania,Phone: +37037326771, fax: +37037326953,E-mail: dserapinas@gmail.com

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Published in „GENETIKA“ Vol. 48, No. 3 (2016), pp. 867 - 880

Serbia

UDC 575

DOI: 10.2298/GENSR1603867B

Original scientific paper

EXPECTED GENETIC ADVANCE AND STABILITY OF PHYTIC ACID AND ANTIOXIDANTS CONTENT IN BREAD AND DURUM WHEAT

Gordana branković¹, Vesna dragičević², Slađana žilić², Desimir knežević³,

Nenad đurić⁴, Dejan dodig²

¹University of Belgrade, Faculty of Agriculture, Belgrade, Serbia

²Maize Research Institute “Zemun Polje”, Belgrade, Serbia

³University of Priština, Faculty of Agriculture, Zubin Potok, Serbia

⁴ Megatrend University, Faculty of Biofarming, Bačka Topola, Serbia

Abstract

branković G., V. Dragičević, S. žilić, D. knežević, N. đurić, D. Dodig (2016): Expected genetic advance and stability of phytic acid and antioxidants content in bread and durum wheat.- Genetika, Vol 48, No.3, 867 - 880.

Fifteen genotypes of bread wheat (Triticum aestivum L.) and fifteen genotypes of durum wheat (Triticum durum Desf.) were evaluated in the multi-environment trial during 2010-11. and 2011-12 vegetation seasons to investigate components of variance, heritability in a broad sense (h²), expected genetic advance (GA), and stability of phytic acid (PA), inorganic phosphorus (P_i), phytic phosphorus (P_p)/P_i relation, yellow pigment (YP), water soluble phenolics (WSPH) and free protein sulfhydryl groups (PSH) content. The field trials were carried out at three locations in Serbia, as randomized complete block design with four replications. The genetic component of variance (σ²_g) predominated the genotype × environment interaction (σ²_ge) component for: P_i in bread wheat (3.0 times higher), P_p/P_i in bread wheat (2.1 times higher) and in durum wheat (1.2 times higher), YP content in bread wheat (2.2 times higher) and in durum wheat (1.7 times higher), and WSPH content in bread wheat (1.4 times higher). The relation σ²_g/σ²_ge for P_i content in durum wheat was equal to one. The σ²_ge prevailed σ²_g for: PA in bread wheat (1.7 times higher) and in durum wheat (5.7 times higher), PSH in durum wheat (3.7 times higher), and WSPH in durum wheat (5.2 times higher). High h² coupled with high expected genetic advance as percent of mean (GAM) were observed for: P_i (93.7% and 26.1%, respectively) in bread wheat, P_p/P_i relation in bread wheat (92.4% and 20.7%, respectively) and in durum wheat (87.2% and 20.8%, respectively), YP content in bread wheat (92.6% and 28.0%, respectively) and in durum wheat (90.7% and 28.1%, respectively), and WSPH content (88.9% and 25.8%, respectively) in bread wheat. PA content in bread and durum wheat had medium to medium high h²(50.5% and 77.9%, respectively), and low expected GAM (9.9% and 3.7%, respectively). GGE biplots with average-environment coordination (AEC) indicated less stability of durum wheat for PA, WSPH and PSH content.

Keywords: Triticum aestivum, Triticum durum, phytic acid, antioxidants, expected genetic advance, GGE (AEC) biplot

Corresponding author: Gordana Branković, University of Belgrade, Faculty of Agriculture, Nemanjina 6, 11000 Belgrade, Serbia, E-mail: gbrankovic@agrif.bg.ac.rs, Phone: +381112615315 extension 2239, Fax: + 381 11 3161 987

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Published in „GENETIKA“ Vol. 48, No.3 (2016), pp 881 -892

Serbia

UDC 575.630

DOI: 10.2298/GENSR1603881C

Original scientific paper

GENETIC VARIABILITY AND INTERRELATIONSHIP AMONG MORPHOLOGICAL AND YIELD TRAITS IN LINSEED (Linum usitatissimum L.)

Chandrawati^1,2, Neha SINGH¹, Vrijesh Kumar YADAV^1,2, Rajendra KUMAR³, Sujit KUMAR³

and Hemant Kumar YADAV^1,2,*

¹CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow-226001

²Academy of Scientific and Innovative Research, New Delhi

³Uttar Pradesh Council of Agricultural Research (UPCAR), Vibhuti Khand, Gomatinagar, Lucknow-226010

Abstract

Chandrawati, N. Singh, R. Kumar, S. Kumar and H. K.Yadav (2016): Genetic variability and interrelationship among morphological and yield traits in linseed (Linum usitatissimum L.).- Genetika, Vol 48, No.3, 881 - 892.

Fifty eight genotypes of linseed (Linum usitatissimum L.) were evaluated two consecutive years for variability and other genetic parameters based on various traits. Substantial amount of genetic variations were observed with low influence of environment indicated consistence performance of the genotypes. High heritability, GCV, PCV and GA was observed for number of branches/plant, number of capsules/plant, days to 50% flowering and capsule weight/plant. Oil content showed high heritability with low genetic advance indicating non-additive genetic behavior. Positive and significant correlations were observed among days to 50% flowering, plant height, number of branches/plant. Seed weight had significant and positive correlation with number of capsules/plant, capsules weight/plant, husk weight/plant and test weight. The branches/plant, number of capsules/plant, and plant height identified as important traits for selection in linseed breeding program.

Keywords: genetic advance, genetic variability, heritability, linseed, path analysis

Corresponding author: Hemant Kumar Yadav, CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow-226001, Tel.:+91-522-2297938, Fax: +91-522-2205836, Email: h.yadav@nbri.res.in

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Published in „GENETIKA“ Vol. 48 No.3 (2016), pp. 893-902

Serbia

UDC 575

DOI: 10.2298/GENSR1603893K

Original scientific paper

LACK OF ASSOCIATION BETWEEN GASTRIC CANCER AND HOPQ ALLELES

IN Helicobacter pylori

Elham kazemi¹ and Danial kahrizi^2,3*

1-Department of Sexual Medicine, The Rhazes Center for Research in Family Health and Sexual Medicine; Kermanshah University of Medical Sciences, Kermanshah, Iran

2. Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran

3- Department of Agronomy and Plant Breeding, Faculty of Agriculture, Razi University, Kermanshah, Iran

Abstract

kazemi E. and D. kahrizi (2016): Lack of association between gastric cancer and HOPQ alleles in Helicobacter pylori.- Genetika, Vol 48, No.3, 893- 902.

Helicobacter pylori use a number of mechanisms to survive in the stomach lumen. The presence of these bacteria in the stomach can lead to gastritis and reduction in stomach acid production. Acute inflammation can directly damage to the peripheral cells that are responsible for the secretion of acid. The risk of developing gastric carcinoma is associated to heterogeneity of Helicobacter pylori virulence factors (such as cagA). The HopQ is one of the outer membrane proteins involved in bacterial adherence to gastric mucosa and has been suggested to also play a role in the virulence of H. pylori. This gene has been shown in two types. The purpose of the current study was to investigate the association between different H. pylori virulence hopQ alleles (types I and II) and patients with gastroduodenal disorders. For this purpose 58 stomach biopsies the of patients with gastric cancer and 100 saliva samples from healthy individuals were collected. Then genomic DNA was purified and PCR for was done for desired genes via specific primers. The H. pylori infections were diagnosed by PCR for GlmM gene. Then frequencies of hopQI+, hopQII⁺ and hopQI⁺ hopQII⁺ genotypes were determined in H. pylori infected cases. Statistical analysis showed that there were not significant differences between healthy and diseased ones for genotypes hopQI⁺, hopQII⁺ and hopQI⁺ hopQII⁺.

Keywords: gastric cancer, HopQ genotyping, Helicobacter pylori

Corresponding author: Danial kahrizi, Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran, Email: dkahrizi@yahoo.com,Tel: +98 918 3322235, Fax: +98 83 3832 10 83

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Published in „GENETIKA“ Vol. 48, No.3 (2016), pp. 903-921

Serbia

UDC 575

DOI: 10.2298/GENSR1603903L

Original scientific paper

EFFECTS OF SOYBEAN CARBOHYDRATES AND Lactobacillus helveticus BGRA43

ON METABOLIC PROCESSES IN RAT COLON

Jovanka lukic¹, Miroslav dinic¹, Mirjana marcetic², Marina milenkovic²,

Jelena begovic¹, Ivana strahinic¹^*

¹Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade, Serbia

²Faculty of Pharmacy, University of Belgrade, Serbia

Abstract

lukic J., M. dinic, M. marcetic, M. milenkovic, J. begovic, I. strahinic (2016): Effects of soybean carbohydrates and Lactobacillus helveticus BGRA43 on metabolic processes in rat colon.- Genetika, Vol 48, No.3, 903 - 921.

Aim of this work was to assess the metabolic and physiological changes that occurred in the hind gut of rats after feeding with soybean carbohydrates alone and in combination with Lactobacillus helveticus BGRA43. Wistar rats were gavaged with soybean flour for 28 days. The parameters assessed included fecal volatile organic compounds, and L-lactate, reducing sugars, proteins, ammonia and water levels in the colonic lumen. The presence of lactic acid (LAB), sulfate reducing (SRB) and methanogenic bacteria was assessed by semi-quantitative PCR. Malondialdehyde levels as well as lymphoid tissue size in ileal and colonic mucosa were also evaluated. On the basics of the results obtained, correlation network was created, setting the parameters tested in research in two metabolic groups: saccharolytic and proteolytic fermentation group. The principal finding of the study is a negative correlation between oral administration of BGRA43 and increase of parameters related to carbohydrate fermentation in the gut, and a positive correlation to factors related to proteolytic fermentation. On the contrary, soybean carbohydrates were correlated with increased values of factors related to carbohydrate catabolism. Different effects of BGRA43 and soybean carbohydrates on metabolic processes in colonic lumen indicate the possibility of applying the BGRA43 in alleviating the gastrointestinal symptoms occurring after consuming hardly digestible carbohydrates.

Keywords: fermentation, lactate, metabolic network, probiotics, reducing bacteria, sulfate

Corresponding author: Ivana Strahinic, Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade, V. Stepe 444a, P. O. Box 23, 11010 Belgrade, Serbia,Telephone: +381 (0)11 3975 960; Fax: +381 (0)11 3975 808; E-mail: strahi@imgge.bg.ac.rs

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Published in „GENETIKA“ Vol. 48, No. 3 (2016), pp. 923 - 932

Serbia

UDC 575.630

DOI: 10.2298/GENSR1603923B

Original scientific paper

Omer beyhan¹, Ecevit eyduran², Meleksen akın³, Sezai ercisli⁴, Mustafa Kenan gecer⁵, Ahmet Erhan karahan⁶

^{^[1]}Sakarya University, Pamukova Vocational School, Sakarya-Turkey

²Igdır University, Agricultural Faculty, Department of Animal Science, Igdir-Turkey

³Oregon State University, Agricultural Faculty, Department of Horticulture, Corvallis, Oregon-USA

⁴Ataturk University, Agricultural Faculty, Department of Horticulture, Erzurum-Turkey

⁵Igdir University, Agricultural Faculty, Department of Horticulture, Igdir-Turkey

⁶Ege University, Agricultural Faculty, Department of Animal Science, Izmir-Turkey

Abstract

beyhan O., E. eyduran, M. akın, S. ercisli, M. K. gecer, A. E. karahan (2016): Identification of the relationship between some characteristics of native walnut genotypes peculiar to Darende district of Malatya province: use of factor analysis scores in multiple linear regression.- Genetika, Vol 48, No.3, 923 - 932.

Two main aims of this investigation were to predict kernel ratio (KR) and kernel weight (KW) from some walnut characteristics, respectively. For these aims, a total of 112 Walnut genotypes growing in nature were collected at Darende District of Malatya province in the Eastern Anatolia region of Turkiye. The walnut characteristics evaluated were nut length (NL), nut width (NW), nut height (NH), nut weight (NWe), shell thickness (ST), kernel ratio (KR) and kernel weight (KW), respectively. Independent variables were subjected to factor analysis based on principal component extraction method and VARIMAX rotation. On the basis of jointly using factor scores in multiple regression, KR (81.3 % R²and 80.6 % adjusted R²) and KW (94.7% R² and 94.5% adjusted R²) characteristics were predicted by using four factor scores with a big accuracy without multicollinearity problem.

Consequently, the present results revealed that, walnuts of heavier KW and NWe in the prediction of KR would be expected to produce those of higher KR, and walnuts of higher values in NH, NW, NWe, ST, NL, and KR in the prediction of KW would be expected to produce those of heavier KW. The knowledge may help walnut breeders to improve new selection strategies.

Keywords: Walnuts; Pomology; Multiple linear regression, Factor Analysis

Corresponding author: Ecevit Eyduran, Igdır University, Agricultural Faculty, Department of Animal Science, Igdir-Turkey, E-mail: ecevit.eyduran@gmail.com

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Published in „GENETIKA“ Vol. 48, No. 3 (2016), pp.933 - 943

Serbia

UDC 575

DOI: 10.2298/GENSR1603933A

STUDY OF KILLER CELL IMMUNOGLOBULIN-LIKE RECEPTOR GENES AND HLA C LIGANDS IN HEMATOPOIETIC STEM CELL TRANSPLANTATION PAIRS IN

VOJVODINA

Dušica S. ademović-sazdanić ¹, Svetlana I. vojvodić ¹,Stevan L. popović²

and Nada V. konstantinidis³

¹Institute for Blood Transfusion of Vojvodina, Department for Laboratory Diagnostics, Tissue Typing Laboratory, University of Novi Sad, Medical Faculty Novi Sad, Novi Sad,Serbia

²Institute for Internal Medicine, Department of Hematology, Clinical Center of Vojvodina, University of Novi Sad, Medical Faculty Novi Sad, Novi Sad, Serbia

³Institute for Child and Youth Health Care of Vojvodina, Department for Oncology and Hematology, University of Novi Sad, Medical Faculty Novi Sad, Novi Sad, Serbia

Abstract

ademović-sazdanić S.D., S. I. vojvodić,S. L. popović and Nada V. konstantinidis (2016): Study of killer cell immunoglobulin-like receptor genes and HLA C ligands in hematopoietic stem cell transplantation pairs in Vojvodina.- Genetika, Vol 48, No.3, 933 - 943.

Objective: The aim of the study was to analyse KIR/HLA profiles and to create the predictive probabilities for the selection of the most suitable Hematopoietic Stem Cell Transplantation (HSCT) donor.

Materials and Methods: The study was conducted on 92 patients with malignant hematological deseases and 181 their first degree relatives, from the region of Vojvodina. HLA and KIR genotyping was performed by polymerase chain reaction-sequence specific primers (PCR-SSP) assay. The analysis included the degree of HLA matching between transplant pairs, number of missing ligands for inhibitory KIR genes, existence of GvH / HvG ligand-ligand mismatches (specific for C1, C2 or Bw4 ligands) and distribution of C1/C1, C1/C2, C2/C2 HLA ligand groups in patients.

Results: There was no significant differences in HLA frequencies between donors and recipients as analyzed by pairwise comparison, the probability of finding HLA identical donor is only 0.154, the probability of finding the donor with ≥ 1 KIR-ligand mismatch is 0.939, the probability of finding the donor with KIR-ligand mismatch for the KIR 2DL1 gene is 0.298, probability of having favorable C1/C1 and C2/C2 HLA ligand groups is 0.565.

Conclusion: The results of our study could be used as a basis for the HSCT outcome prediction representing a powerful tool for choosing the most suitable donor.

Keywords: Hematopoietic Stem Cell Transplantation; Human Leukocyte Antigen; Killer cell Immunoglobulin-like Receptor genes

Corresponding author: Svetlana Vojvodić, Institute for Blood Transfusion of Vojvodina, Department for Laboratory Diagnostics, Tissue Typing Laboratory, Hajduk Veljkova 9a, 21000 Novi Sad, Serbia; Tel.: +381-21-4877-963; Fax:+381-21-4877-978; Mobile:+381-64-861-58-12; email: svetlana.vojvodic021@ gmail.com

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Published in „GENETIKA“ Vol. 48, No.3 (2016), pp. 945 -954

Serbia

UDC 575

DOI: 10.2298/GENSR1603945G

Original scientific paper

SEQUENCE ANALYSIS OF CANDIDATE GENES IN TWO ROMA FAMILIES

WITH SEVERE TOOTH AGENESIS

Dana gabriková¹, Daniela grejtáková¹, Jarmila bernasovská¹,

Anna pavúková², Barbora lewandowská¹, Mariana bindasová¹, Janakisková¹, Ivana klobušovská¹, DanicaHronská¹, Soňa mačeková¹

¹Department of Biology, Faculty of Humanities and Natural Sciences, University of Prešov, Prešov, Slovakia.

²Dental Clinic Šarišské Michaľany, Šarišské Michaľany, Slovakia

Abstract

gabriková D., D. grejtáková, J. bernasovská, A. pavúková, B. lewandowská, M. bindasová, J.kisková, I. klobušovská, D.Hronská, S. mačeková (2016): Sequence analysis of candidate genes in two Roma families with severe tooth agenesis.- Genetika, Vol 48, No.3, 945-954.

Selective tooth agenesis is the most common congenital disorder affecting the formation of dentition in humans. Both its forms (hypodontia and more severe oligodontia) can be found either in isolated form and they can be associated with systemic condition (syndromic tooth agenesis). In addition to previously known genes (PAX9, MSX1 and AXIN2) mutations in EDA, EDARADD and WNT10 gene were recently found to be involved in isolated forms of tooth agenesis. The objective of this study was to characterize the phenotype of affected members in two large families of Roma origin segregating severe isolated tooth agenesis with very variable phenotype and to perform mutation analysis of seven genes with aim to find causal mutation. 26 family members were clinically examined and coding regions of seven genes (MSX1, PAX9, AXIN2, EDA, EDAR, EDARADD and WNT10A) were sequenced. With exclusion of third molars, average number of missing teeth was 8.2 ± 4.9 in family 1 and 7.1 ± 2.3 in family 2. The most frequently missing teeth were maxillary lateral incisors and first premolars and mandibular central incisors. Sequencing revealed four potentially damaging variants (g.Ala40Gly in MSX1, g.Ala240Pro in PAX9, g.Pro50Ser in AXIN2 and g.Met9Ile in EDARADD); however, none of them was present in all affected family members. Variable phenotype in both families examined in this study is in favour of heterogeneous genetic cause of tooth agenesis in these families: possible interaction of several defected genes, sequence variants in regulatory regions and additional environmental factors is assumed.

Keywords: candidate genes, genetic causes,hypodontia, oligodontia, odontogenesis

Corresponding author: Dana Gabriková, Department of Biology, Faculty of Humanities and Natural Sciences, University of Prešov, 17. Novembra 1, 08116 Prešov, Slovakia. E-mail: dana.gabrikova@yahoo.com, Phone: +421949172989, Fax: +421517725547

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Published in „GENETIKA“ Vol. 48, No. 3 (2016), pp. 955-961

Serbia

UDC 575

DOI: 10.2298/GENSR1603955P

Original scientific paper

CONFINED PLACENTAL MOSAICISM IN SHORT TERM CULTURE

Bojana PETROVIĆ, Jelena DUKANAC-STAMENKOVIĆ

Clinic for gynecology and obstetrics, Clinical center of Serbia, Belgrade, Serbia

Abstract

Petrović B. and J. Dukanac-Stamenković (2016): Confined placental mosaicism in short term culture.- Genetika, Vol 48, No.3, 955 -961.

Finding of fetal chromosomal mosaicism complicates genetic counseling, as well as pregnancy management. The aim of this study was to determine the risk of confined placental mosaicism in short term culture of chorionic villous samples. We conducted a retrospective review of karyotype analysis results obtained after chorionic villous sampling (CVS) in two years period. A 420 samples of chorionic villi were taken transabdominally and obtained by a semidirect method (overnight incubating culture). All fetuses with CVS mosaicism were under the intensive perinatal care. In all cases of chromosome mosaicism the additional karyotyping was performed from fetal blood samples after 22^nd gestational week in order to exclude true fetal mosaicism. After delivery newborns were examined by experienced pediatrician. From 420 analyzed samples in 11 (2,6%) cases we found placental mosaicism. No anomalies were seen in genetic sonogram of this fetuses and mosaicism was confirmed only in one case. Confined placental mosaicism (CPM) was found in 2,1% (9/420) of all analyzed cases, and it made 90% of all placental mosaicism. In 60% (6/10) of placental mosaicism cases we found mosaicism with single aberrant cell. Trisomy 21 mosaicism was the most frequent aberration found in 30% of cases. Finding of mosaicism in chorionic villi sample is at special importance for genetic counseling, because every case has to be reveled individually regarding the type and level of mosaicism. Anyway, in every case of placental mosaicism intensive antenatal monitoring is necessary, with additional chromosome analysis from different tissue in consideration of previous findings.

Keywords: confined placental mosaicism, short term culture.

Corresponding author: Bojana Petrović, Bulevar Arsenija Čarnojevića 25/23,11070 Novi Beograd,tel: 0117116810 (home), 0646178320 (mobile),e-mail: bojana.petrovic1977@gmail.com

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Published in „GENETIKA“ Vol. 48, No.3 (2016), pp. 963 - 970

Serbia

UDC 575

DOI: 10.2298/GENSR1603963S

Original scientific paper

DETECTING LEVEL OF GENETIC DIFFERENTIATION IN TWO CLOSELY RELATED SPECIES OF Drosophila: D. bipectinata AND D. malerkotliana

A. K. singh*, Sanjay kumar and Neha singh

Genetics Laboratory, Department of Zoology, Banaras Hindu University,

Varanasi-221005, India

Abstract

singh A. K., S. kumar and N. singh (2016): Detecting level of genetic differentiation in two closely related species of Drosophila: D. bipectinata and D. malerkotliana.- Genetika, Vol 48, No.3, 963 -970.

A considerable amount of allozyme variation exists among different populations of a Drosophila species. Such allozyme variation can also be observed between two closely related species of Drosophila which show reproductive isolation but experience mating under laboratory conditions and produce hybrids. D. bipectinata and D. malerkotliana are two closely related sympatric species and belong to bipectinata species complex. Allozyme polymorphism studies conducted with them and their hybrids reveal that these two species have enough genetic differentiation due to allozyme variation at three enzyme coding loci; however, their hybrids exhibit common allozyme variants of both the species. The hybrids exhibit very little genetic differentiation from either of their parents.

Keywords: allozyme; Drosophila bipectinata; hybrid; genetic differentiation, species

Corresponding author: Prof. A. K. Singh, Genetics Laboratory, Department of Zoology, Banaras Hindu University, Varanasi-221005, India; Email: aksbhu23@rediffmail.com

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Published in „GENETIKA“ Vol. 48, No. 3 (2016), pp. 971- 982

Serbia

UDC 575.630

DOI: 10.2298/GENSR1603971M

Original scientific paper

EFFECT OF DIFFERENT SOIL WATER CONTENT EFFECT ON GENOTYPE EXPESSION IN PHOTOSYNTHETIC EFFICIENCY AND LEAF TEMPERATURE

IN SUNFLOWER

Antonela markulj kulundžić¹, Marija viljevac vuletić¹, Siniša jocić², Sandra cvejić², Maja matoša kočar¹, Anto mijić¹, Ivica liović¹, Aleksandra sudarić¹, Hrvoje lepeduš³, Josip kovačević¹, Ana josipović¹

¹Agricultural Institute Osijek, Osijek, Croatia

²Institute of Field and Vegetable Crops, Novi Sad, Serbia

³Faculty of Humanities and Social Sciences, J. J. Strossmayer University of Osijek, Osijek, Croatia

Abstract

markulj kulundžićA., M. viljevac vuletić, S. jocić, S. cvejić, M. matoša kočar, A. mijić, I. liović, A. sudarić, H. lepeduš, J. kovačević, A. josipović (2016): Effect of different soil water content effect on genotype expession in photosynthetic efficiency and leaf temperature in sunflower.- Genetika, Vol 48, No.3, 971-982.

Sunflower (Helianthus annuus L.) has high needs for water but can tolerate drought very well because, under stress conditions, its well developed root system can supply water and mineral nutrients from deeper soil layers. Reduced water content in soil affects plant growth and development, photosynthetic rate and causes rapid leaf senescence. In this study, we measured maximum quantum yield of photosystem II (F_v/F_m), photosynthetic performance index (PI_ABS) and leaf temperature (LT) on 13 sunflower genotypes at different soil water contents. By calculating stress tolerance indices (STI) of F_v/F_m and PI_ABS parameters we evaluated drought tolerance for every tested sunflower genotype at given soil water contents. The experiment was set up in vegetation pots in two treatments with different soil water contents (60% and 80% of field water capacity) in three replications. Based on the obtained results for F_v/F_m and PI_ABS and STI values of F_v/F_m and PI_ABS parameters, we concluded that genotypes 5 and 12 had higher tolerance at both treatments, as opposed to genotypes 2 and 13 which were less tolerant. These analyses will help breeders to select genotypes adapted to different farming areas which is, along with the use of recommended production practices, the background for profitable sunflower production.

Keywords: drought, Helianthus annuus, maximum quantum yield of photosystem II, photosynthetic performance index, stress tolerance index.

Corresponding author: Antonela Markulj Kulundžić, Agricultural Institute Osijek, Južno predgrađe 17, HR-31103 Osijek, Croatia; Phone: +385 31 515 537; E-mail: antonela.markulj@poljinos.hr

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Published in „GENETIKA“ Vol. 48, No. 3 (2016), pp. 983-990

Serbia

UDC 575:633

DOI: 10.2298/GENSR1603983B

Original scientific paper

VARIABILITY OF HEAD DIAMETER IN SUNFLOWER HYBRIDS DEPENDING

ON PLANTING DATE

Igor balalić¹, Jovan crnobarac², Siniša jocić¹, Vladimir miklič¹,

Velimir radić¹, Nenad dušanić¹

¹Institute of Field and Vegetable Crops, Novi Sad, Serbia

²University of Novi Sad, Faculty of Agriculture, Novi Sad, Serbia

Abstract

balalić I., J. crnobarac, S. jocić, V. miklič, V. radić, N. dušanić(2016): Variability of head diameter in sunflower hybrids depending on planting date. - Genetika, Vol 48, No.3, 983 - 990.

Head size contributes substantially to seed yield of sunflower because it influences both number of seeds per head, and seed size. The aim of this field study was to determine the variability of head diameter in sunflower hybrids depending on planting date across three cropping seasons. Three hybrids (Miro, Rimi, Pobednik) were sown at four planting dates (PD1 - 20^th of March, PD2 - 10^thof April, PD3 - 30^th of April, PD4 - 20^th of May). Head diameter was analysed in the stage of flowering and physiological maturity. The trial was arranged as Randomized Complete Block Design (RCBD) with four replications. Analysis of variance (ANOVA) showed that the effect of year, hybrid, planting date and most interactions were highly significant for head diameter in the flowering stage. In the stage of physiological maturity head diameter varied significantly depending only on the planting date. Also, all interactions, except year × hybrid (Y × H), were highly significant. Planting date had significant influence on head diameter in the stage of flowering and physiological maturity in sunflower. With later sowing head diameter increased, so that significantly highest head diameter across planting dates was found in PD4 (11.8 cm in flowering stage, and 22.6 cm at the stage of physiological maturity). Study results may be helpful in the recommendation for optimal planting date in sunflower.

Keywords: head diameter, hybrid, planting date, sunflower, variability

Corresponding author: Igor Balalić, Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad, Serbia, Phone: +381214898318; Fax: +381216413833; E-mail: igor.balalic@ifvcns.ns.ac.rs

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Published in „GENETIKA“ Vol. 48, No. 3 (2016), pp. 991-1001

S. Bajića 1, 11185 Belgrade-Zemun

Serbia

UDC 575:633.11

DOI: 10.2298/GENSR1603991B

Original scientific paper

ASSESMENT OF WINTER WHEAT ADVANCED LINES BY USE OF MULTIVARIATE STATISTICAL ANALYSES

Dane boshev¹, Mirjana jankulovska¹, Sonja ivanovska¹,

Ljupcho jankuloski¹

¹Faculty of Agricultural Sciences and Food, Ss. Cyril and Methodius University in Skopje, Republic of Macedonia

Abstract

boshev D., M. jankulovska, S. ivanovska, Lj. jankuloski (2016): Assessment of winter wheat advanced lines by use of multivariate statistical analyses.- Genetika, Vol 48, No.3, 991- 1001.

This study was conducted to evaluate 49 advanced lines of winter wheat (Triticum aestivum L.) for their morphoagronomic traits and to determine best criteria for selection of lines to be included in future breeding program. The material was assessed in two years experiment at two locations, using RCBD design with three replications. Ten quantitative traits: plant height, number of fertile tillers, spike length, number of spikelets per spike, number of grains per spike, weight of grain per spike and per plant, fertility, biological yield and harvest index were evaluated by PCA and two-way cluster analysis. Three main principal components were determined explaining 71.391% of the total variation among the genotypes. One third of the variation is explained by PC1 which reflects the genotype yield potential. PC2 and PC3 explained 25.22% and 15.49% of the total variance, mostly in relation to the plant height and spike components, respectively. Biplot graph revealed strongest positive association between spike length, number of spikelets and biological yield and between number of tillers, weight of grains per spike and per plant. Two-way cluster analysis resulted with a dendrogram with one solely separated genotype, superior for all traits and two main clusters of genotypes defined with wide genetic diversity especially between the groups within the second cluster. Genotypes with high values for specific traits will be included in the future breeding programmes. Classification of genotypes and the extend of variation among them illustrated on the heatmap has proved to be practical tool for selecting genotypes with desired traits in the early stages of the breeding process.

Keywords: advanced lines, cluster analysis, PCA, productive traits, wheat

Corresponding author: Dane Boshev, Faculty of Agricultural Sciences and Food, blvd. Aleksandar Makedonski BB, 1000 Skopje, Macedonia, Republic of Macedonia, tel: ++389 2 3115 277, fax: ++389 2 3134 210, e-mail: dboshev@yahoo.com.

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Published in „GENETIKA“ Vol. 48, No. 3(2016), pp. 1003-1015

S. Bajića 1, 11185 Belgrade-Zemun

Serbia

UDC 575:630

DOI: 10.2298/GENSR1603003J

Original scientific paper

GENETIC VARIABILITY OF FREE ENERGY IN A FUNCTION OF DROUGHT TOLERANCE IN COMMON BEAN ACCESSIONS

Maja ječmenica¹, Natalija kravić², Mirjana vasić³, Tomislav živanović⁴,

Violeta mandić⁵, Jelena damnjanović⁶, Vesna dragičević²

¹Ministry of Agriculture and Environment, Directorate for National Reference Laboratories, Belgrade, Serbia

²Maize Research Institute „Zemun Polje“, Belgrade, Serbia

³Institute of Field and Vegetable Crops, Novi Sad, Serbia

⁴University of Belgrade, Faculty of Agriculture, Belgrade, Serbia

⁵Institute for Animal husbandry, Zemun, Serbia

⁶Institute for vegetable crops, Smederevska Palanka, Serbia

Abstract

Ječmenica M., N. Kravić, M. Vasić, T. Živanović, V. mandić, J. damnjanović, V. Dragičević (2016): Genetic variability of free energy in a function of drought tolerance in common bean accessions. - Genetika, vol 48, no. 3, 1003 - 1015.

Characterisation of bean genotypes, particularly local landraces is important for ongoing breeding programs, especially for drought tolerance. Susceptibility to drought is emphasized when bean is grown as a stubble crop and sown at the middle of summer. The aim of this study was to compare variability of ten bean genotypes to optimal (25 °C) and higher (30 °C) temperatures in combination with optimal (80%) and reduced (40%) field water capacity (FWC), from the point of growth (root and shoot length and fresh matter accumulation) and thermodynamic parameters of free energy (calculated parameter after drying at 60 °C, 105 °C and 130 °C) during the early seedlings stage. Significant and positive correlation between root length and fresh matter with free energy at symplast and chemically bound water occurred under temperature stress (i.e. 30 °C). Root growth and elongation were affected by drought stress, i.e. under a combination of high temperature (30 °C) and water deficit (40% FWC). Based on higher energy consumption, the ability of shoot to continue a growth in stressful conditions could be possible to achieve, as was confirm through significant and positive correlation between evaluated growth parameters in shoot and free energy of free water. It could be concluded that most of the examined dry bean accessions expressed some sensitivity to stress applied. Among genotypes tested, local landrace zecak expressed lesser susceptibility to stresses applied. Accession Maksa was more tolerant to changes at cytoplasmic level, while Medijana and Sataja 425 expressed root i.e. shoot stress tolerance. Those accessions could be considered as potentially drought tolerant genotypes.

Keywords: field water capacity, free energy, genotype, temperature, water deficit, Phaseolus vulgaris L.

Corresponding author: Vesna Dragičević, Maize Research Institute, S.Bajica 1, Belgrade, 11185 Serbia, e-mail: vdragicevic@mrizp.rs, Phone: +381 11 37 56 704, Fax: +381 11 37 56 707

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Published in „GENETIKA“ Vol. 48, No. 3(2016), pp. 1017-1025

Serbia

UDC 575

DOI: 10.2298/GENSR1603017U

Original scientific paper

MOLECULAR CHARACTERIZATION OF INDIGENOUS OLIVE GENOTYPES BASED ON SSR ANALYSIS

Hulya unver¹, Ebru sakar^2*, Mehmet ulas³, Sezai ercisli⁴, Bekir Erol ak²

¹Ankara University Kalecik Vocational School Kalecik-Ankara, Turkey

²Harran University Agricultural Faculty, Department of Horticulture Sanliurfa, Turkey

³Bornova Olive Research Institute, Izmir, Turkey

⁴Atatürk University, Agricultural Faculty, Department of Horticulture Erzurum, Turkey

Abstract

unver H., E. sakar, M. ulas, S.ercisli, B. E. ak (2016): Molecular characterization of indigenous olive genotypes based on SSR analysis. - Genetika, Vol 48, No.3, 1017 - 1025.

Trees of 25 widely grown olive genotypes were analyzed using a set of 10 SSR (simple sequence repeat) primer pairs and to evaluate genetic diversity and reveal inter-cultivar relationships. Two well-known international olive cultivars (Chetoni and Manzanilla) and four widely grown Turkish standard cultivars (Aycalik, Edincik Su, Gemlik, Kilis Yaglik) are also included in the study to compare Kilis genotypes. The 10 polymorphic SSR loci exhibited 4 (UDO4) to 17 alleles (UDO43), with expected heterozygozity (He) ranging from 0.510 to 0.887 and a mean of 0.692 presenting high polymorphism. In this study we did not determine identical genotypes and Polateli4 and Kilis Yağlık (0.75), Polateli3 and Polateli7 (0.75) and Polateli6 and Manzanilla (0.70) revealed the highest similarity ratio each other. The most genetically divergent cultivars were Elbeyli8 and Musabeyli5 (0.10); Elbeyli3 and Musabeyli7 (0.15) and Musabeyli6 and Elbeyli7 (0.15), respectively

Keywords: molecular characterization, olive, SSR

Corresponding author: Ebru Sakar, Harran University Agricultural Faculty Department of Horticulture 63300 Sanliurfa-Turkey, Phone: 0090 4143183708, Fax: 0090 4143183682, e-mail: ebru.sakar@harran.edu.tr

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Published in „GENETIKA“ Vol. 48, No.3 (2016), pp. 1027-1038

Serbia

UDC 575.630

DOI: 10.2298/GENSR1603027B

Original scientific paper

AMMI MODEL IN THE ANALYSIS OF GENOTYPE BY ENVIRONMENT INTERACTION OF CONVENTIONALLY AND ORGANICALLY GROWN ONION

Milka BRDAR-JOKANOVIĆ¹, Zdenka GIREK², Milan UGRINOVIĆ², Vladimir SIKORA¹, Ivica ĐALOVIĆ¹, Jasmina ZDRAVKOVIĆ²

¹Institute of Field and Vegetable Crops, Novi Sad, Serbia

²Institute for Vegetable Crops, Smederevska Palanka, Serbia

Abstract

Brdar-Jokanović M., Z. Girek, M. Ugrinović, V. Sikora., I. Đalović, J. Zdravković (2016): AMMI model in the analysis of genotype by environment interaction of conventionally and organically grown onion.- Genetika, Vol 48, No.3, 1027 - 1038.

This study was aimed to assess the stability of direct yield components (bulb weight and number plot^-1) and other yield contributing characteristics (bulb diameter, height and index, neck diameter and length, plant height, emergence and vegetation period) in five commercial onion cultivars grown in conventional and organic environments, by employing additive main effect and multiplicative interaction (AMMI) statistical model in data analysis. The two-year field trial organized in complete randomized blocks included the plots maintained in four regimes: mineral fertilization (conventional), without fertilization, fertilization with farmyard manure and with bacterial fertilizer (organic). Each treatment by year combination was considered as an environment. Analysis of variance of AMMI model calculated for the investigated traits showed that all sources of variation (genotypes, environments, genotype by environment interaction) were highly significant. The largest proportions of the total sum of squares were encompassed by environments, except for emergence and bulb index with the pronounced effect of genotypes (67.26 and 52.54%, respectively) and neck length with the genotype by environment interaction amounting 44.59%. Generally, the effects of the interactions were in the common range. The AMMI model with two axes was concluded as the best model for the investigated traits. Onions grown in conventional system outperformed the organic ones. However, good performance of the genotypes was accompanied with low stability across the environments and vice versa. Therefore breeding programs intended to develop cultivars adapted to alternative production systems should rely on the experiments set in the corresponding environments that include various combinations of genotypes and agro-technical procedures based on the principles of organic agriculture.

Keywords: AMMI analysis, genotype by environment interaction, onion, organic agriculture

Corresponding author: Milka Brdar-Jokanović, Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad, Serbia, Phone: 381 21 780 365, Fax: 381 21 780 198, E-mail: milka.brdar@ifvcns.ns.ac.rs, milka.brdarjokanovic@gmail.com

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Published in „GENETIKA“ Vol. 48, No. 3(2016), pp. 1039-1052

Serbia

UDC 575

DOI: 10.2298/GENSR1603039T

Original scientific paper

SYNERGISTIC EFFECT OF ENVIRONMENTAL AND GENOMIC STRESS ON WING SIZE OF Drosophila subobscura

Marija tanasković¹, Zorana kurbalija novičić², Bojan kenig¹, Marija savić veselinović³, Marina stamenković-radak³, Marko anđelković⁴

¹University of Belgrade - Institute for Biological Research “Siniša Stanković”, Belgrade, Serbia

²Animal Ecology, Department of Ecology and Genetics, Evolutionary Biology Center, Uppsala University, Uppsala, Sweden

³Faculty of Biology, University of Belgrade, Belgrade, Serbia

⁴Serbian Academy of Sciences and Arts, Belgrade, Serbia

Abstract

tanasković M., Z. kurbalija novičić, B. kenig, M.savić veselinović, M.stamenković-radak, M. anđelković (2016): Synergistic effect of environmental and genomic stress on wing size of Drosophila subobscura.- Genetika, Vol 48, No.3, 1039 - 1052.

Growing anthropogenic influence on every aspect of environment arise important issues regarding the ability of populations and species to adapt to variant pressures. Lead is one of the most present contaminants in the environment with detrimental influence on organisms and populations. In combination with genomic stress, lead may act synergistically, leading to reduction in adaptive values. We sampled two Drosophila subobscura populations, from ecologically different habitats and established differences in genetic backgrounds and population histories. In order to establish different levels of genome heterozygosity, series of intra-line, intra-population and between population crosses were made. The progeny was reared on a standard Drosophila medium and a medium with 200μg/mL of lead acetate and right wing of approximately 4000 individuals was used for geometric morphometric analysis of wing size. Results showed that lead significantly reduces wing size and that magnitude of this reduction is dependent on genetic background, indicating synergistic effect of genomic and environmental stress. There is also an indication of strong female origin influence on the outcome of hybridization when source of environmental stress is lead. Our results showed that the genetic structure of populations is of great importance for population fitness in anthropogenic induced stressful conditions. Further studies of synergistic effect of genetic and environmental stress are needed, as well as studies of its outcome in natural populations.

Keywords: Drosophila subobscura, hybridization, lead pollution, synergistic effect, wing size

Corresponding author: Marija Tanasković, Department of Genetics of Populations and Ecogenotoxicology, Institute of Biological Research “Siniša Stanković”, University of Belgrade, Despot Stefan Blvd.142, 11060 Belgrade, Serbia; Phone number: +381 11 20 78 328; Fax number: +381 11 27 61 433; email: marija.tanaskovic@ibiss.bg.ac.rs

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Published in „GENETIKA“ Vol. 48, No. 3(2016), pp. 1053-1066

Serbia

UDC 575.633.15

DOI: 10.2298/GENSR1603053E

Original scientific paper

MORPHO-PHYSIOLOGICAL COMBINING ABILITY AMONG TROPICAL AND TEMPERATE MAIZE GERMPLASM FOR DROUGHT TOLERANCE

Sekip erdal¹, Mehmet pamukcu¹, Ahmet ozturk¹, Koksal aydinsakir¹,

Ozlemyilmaz dogu²

¹Bati Akdeniz Agricultural Research Institute-Antalya, Turkey

²General Directorate of Agricultural Research and Policies, Ankara, Turkey

Abstract

erdal S., M. pamukcu, A. ozturk, K. aydinsakir, O.yilmaz dogu (2016): Morpho-physiological combining ability among tropical and temperate maize germplasm for drought tolerance.- Genetika, vol 48, no. 3, 1053 – 1066.

In this study, seven maize inbred lines representing different tropical, drought tolerant populations and two adapted temperate maize inbred lines were crossed in a half-diallel mating design to determine combining abilities. The genotypes were tested in well-watered (WW) and managed water stressed (WS) conditions in 2013 and 2014. General combining ability (GCA) and specific combining ability (SCA) mean squares were significant for all investigated traits and demonstrated both additive and non-additive genetic effects in both conditions. Higher desired leaf rolling, leaf senescence, stomatal conductance and leaf chlorophyll content GCA effects of tropical inbreds under WS conditions showed the presence of the valuable allels related to drought stress. SCA analysis revealed that the best hybrids for water use efficiency and irrigation water use efficiency were tropical x temperate crosses. Stress tolerance index and drought resistance index identified G5 x G9, a tropical x temperate hybrid, as the most tolerant hybrid to drought. Our study suggests that tropical drought tolerant germplasm has the potential to contribute useful genetic diversity to temperate maize breeding programs.

Keywords: diallel analysis, drought tolerance, maize, temperate, tropical

Corresponding author: Sekip Erdal, Bati Akdeniz Agricultural Research Institute-Antalya, Turkey, sekip.erdal@gthb.gov.tr, +902424297331

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Published in „GENETIKA“ Vol. 48, No. 3(2016), pp. 1067-1076

Serbia

UDC 575.633.15

DOI: 10.2298/GENSR603067N

Original scientific paper

ESTABLISHMENT AND CONFIRMATION OF HETEROTIC GROUPS AND GENETIC DIVERSITY ASSESSMENT OF MAIZE INBRED LINES USING MICROSATELLITE DATA

Ana nikolić, Jelena bogosavljević, Zoran čamdžija, Milomir filipović,

Dragan kovačević, Milan STEVANOVIĆ, Snežana mladenović drinić

Maize Research Institute “Zemun Polje”, Belgrade, Serbia

Abstract

nikolić A. J. bogosavljević, Z. čamdžija, M. filipović, D. kovačević, M. Stevanović, S. mladenović drinić (2016): Establishment and confirmation of heterotic groups and genetic diversity assessment of maize inbred lines using microsatellite data. - Genetika, Vol 48, No.3, 1067 - 1076.

Twenty-seven maize inbreds (12 commercial and 15 developing lines) from Maize Research Institute breeding program were subjected to microsatelite analysis. The aim was genetic diversity determination, establishing relationships among tested lines and assigning them to heterotic groups according to molecular marker data. Number of alelles detected was 97, with an average of 3.23. Major allele frequency was in a range from 0.33 to 0.82 (average 0.55). The highest value for observed heterozygosity was 10% for several developing lines. Mean values for gene diversity and PIC were 0.56 and 0.48, respectively. Frequency-based distances were calculated using Roger’s coefficient and average value of 0.57 indicates high genetic diversity in analyzed maize inbreds. Distance matrices were subjected to cluster analysis and PCA. Multivariate analysis methods showed considerable concurrency with pedigree data. Results of analysis with 30 microsatellite markers could be useful for defining/redefining heterotic groups but should be complemented with field testing data.

Keywords: genetic diversity, heterotic groups, maize, SSR

Corresponding author: Ana Nikolić, Maize Research Institute “Zemun Polje”, Slobodana Bajića 1, 11185 Zemun Polje, Serbia, E-mail: anikolic@mrizp.rs, Phone: + 381 64 840 6216

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Published in „GENETIKA“ Vol. 48, No. 3(2016), pp 1077-1086

Serbia

UDC 575:630

DOI: 10.2298/GENSR1603077M

Original scientific paper

APPLYING MENDELIAN RULES IN RAPESEED (Brassica napus) BREEDING

Ana marjanović jeromela¹, Aleksandra dimitrijević¹, Sreten terzić¹, Aleksandar mikić¹, Jovanka atlagic¹, Dragana miladinović¹, Mirjana jankulovska²,

Jasna savić³, Wolfgang friedt⁴

¹Institute of Field and Vegetable Crops, Novi Sad, Serbia

²Faculty of Agricultural Sciences and Food, University of Skopje, Republic of Macedonia

³Faculty of Agriculture, University of Belgrade, Belgrade-Zemun, Serbia

⁴Justus Liebig University, Institute of Agronomy & Plant Breeding, Giessen, Germany

Abstract

marjanović jeromela A., A. dimitrijević, S. terzić, A. mikić, J. atlagic, D. miladinović, M. jankulovska, J. savić, W. friedt (2016): Applying Mendelian rules in rapeseed (Brassica napus) breeding.- Genetika, Vol 48, No.3, 1077 - 1086.

Rapeseed is one of the most important sources of edible oil, raw material for industry, as well as feed. The yield and quality of rapeseed have significantly been improved in recent decades as a result of intensive breeding and optimized production technology. The application of Mendel's rules in introducing monogenic traits has also contributed to success in rapeseed breeding. Rule 1, which refers to the uniformity of F₁ generation, is now the basis of widespread development of rapeseed hybrids. Rule 2, dealing with genetic segregation in the F₂ generation, is the basis for understanding the process of breeding lines. Rule 3, regarding the independent segregation of genes and traits, while exempting linked traits, is the basis of combining different desirable properties by selection. In the last few decades, the systematic use of Mendel's rules has contributed to the improvement of many properties of rapeseed, including tolerance to biotic and abiotic stress, yield and seed quality. Particular progress has been made in breeding for resistance to diseases, including the identification of molecular markers for marker-assisted selection. The next objective of rapeseed breeding is to create varieties with improved tolerance to environmental stress (e.g. frost, heat, and drought). Based on Mendel's rules, classical breeding methods and the latest developments in the field of molecular genetics and breeding, future progress is expected in the field of rapeseed breeding with an emphasis on polygenic, quantitative traits such as biomass, seed, and oil yield.

Keywords: applied genetics; Brassica napus; breeding; fatty acid composition; grain quality; grain yield; Mendelian traits; protein meal; rapeseed

Corresponding author: Ana Marjanović Jeromela, Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad, Serbia, (phone) +381214898405 and +381648205739, (fax) +381216413833, (e-mail) ana.jeromela@ifvcns.ns.ac.rs

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Published in „GENETIKA“ Vol. 48, No. 3(2016), pp. 1087-1100

UDC 575.630

DOI: 10.2298/GENSR1603087A

Original scientific paper

DROUGHT STRESS EFFECTS ON THE GRAIN YIELD AMONG DIFFERENT BARLEY CULTIVARS

Aram ARSHADI¹, Ezzat KARAMI^2*, Behzad KHATERI³, Payam REZABAKHSH⁴

¹Department of Plant Breeding, Campus of Agriculture and Natural Resources, Razi University, Kermanshah, Iran

²Department of Agronomy and Plant Breeding, College of Agriculture and Natural Resources, Sanandaj Branch, Islamic Azad University, Sanandaj, Kurdistan, Iran

³ Department of Biotechnology, Maragheh Branch, Islamic Azad University, Maragheh, Iran

⁴Department of Agroecology, Mahabad Branch, Islamic Azad University, Mahabad, Iran

Abstract

Arshadi A., E. Karami, B. Khateri, P. Rezabakhsh (2016): Drought stress effects on the grain yield among different barley cultivars. - Genetika, Vol 48, No.3, 1087 - 1100.

We examined commercial barley cultivars in two sequential growing season by including irrigation and rainfed conditions under a randomized complete block experimental design. There were positive and significant correlations between the yeild and either of the indices including Mean productivity (MP), Stress Tolerance Index (STI), Geometric Mean Productivity (GMP), Harmonic Mean Productivity (HMP), and Mean Relative Performance (MRP) in both irrigated and drought conditions. The PC1 and PC2 justified 94.86 and 94.44% of total variation in the first year and second year, respectively. According to 3-dimensional diagram and based on STI, genotypes 2, 3, and 8 in the first year and gentypes 4, 3, 8, and 2 in the second year were clustered together in group A, and therefore, called the most tolerant cultivars to drought stress.

Keywords: barley, biplot, drought stress, drought tolerance indices

Corresponding author: Ezzat Karami, Assistant Professor of Plant Breeding, Department of Agronomy and Plant Breeding, College of Agriculture and Natural Resources, Sanandaj Branch, Islamic Azad University, Sanandaj, Kurdistan, Iran,Email address: Ezzatut81@yahoo.com

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Published in „GENETIKA“ Vol. 48, No.3(2016), pp. 1101-1110

Serbia

UDC 575.630

DOI: 10.2298/GENSR1603101G

Original scientific paper

DIVERSITY IN CHEMICAL COMPOSITION AND YIELD OF ESSENTIAL OIL FROM TWO MENTHA SPECIES

Ahmad Reza GOLPARVAR^1*, Amin HADIPANAH²

¹Department of Agronomy and Plant Breeding, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran

²Department of Horticultural, Science and Research Branch, Islamic Azad University, Tehran, Iran

Abstract

Golparvar R. A. and A. Hadipanah (2016): Diversity in chemical composition and yield of essential oil from two Mentha species.- Genetika, Vol 48, No.3, 1101 - 1110.

The genus Mentha, which belongs to the mint family (Lamiaceae). Essential oil yield and chemical components of two Mentha species including Mentha longifolia (L.) Huds. and (Mentha spicata L.) collected from three ecotypes in Iran were investigated. The essential oils of samples were obtained by hydro-distillation, and analyzed using gas chromatography–mass spectrometry (GC–MS). A significant difference (p < 0.05) in oil yields was obtained from the aerial parts of two Mentha species. The essential oil yields were obtained from the aerial of M. longifolia, 0.62, 0.85 and 1.24 ml / 100 g dry matter identified in Ardestan, Saman and Kuhrang province, respectively and the aerial of M. spicata, 0.49, 1.02 and 1.54 ml / 100 g dry matter identified in Ardestan, Saman and Kuhrang province, respectively. Results indicated significant differences (p < 0.01) among the aerial for the main constituents in the essential oil from two Mentha species. The major constituents of the essential oil from the aerial of M. longifolia collected from Ardestan province were pulegone (31.21%), 1,8-cineole (23.01%), sabinene (6.76%), the aerial of M. longifolia collected from Saman province were pulegone (31.06%), 1,8-cineole (24.34%), sabinene (7.45%) and the aerial of M. longifolia collected from Kuhrang province were pulegone (36.42%) and 1,8-cineole (29.49%). The major constituents of the essential oil from the aerial of M. spicata collected from Ardestan province were 1,8-cineole (35.28%), carvone (30.71%), the aerial of M. spicata collected from Saman province were carvone (35.37%), 1,8-cineole (24.35%), pulegone (18.67%) and the aerial of M. spicata collected from Kuhrang province were carvone (41.51%), 1,8-cineole (25.95%). Generally, a comparison of our results with the previous reports suggests differences in the essential oil compositions and oil yield of the plant material could be attributed to genetic diversity in two Menthe species.

Keywords: chemical constitutes, genetic diversity, Mentha longifolia L., Mentha spicata L.

Corresponding author: Ahmad Reza Golparvar, Department of Agronomy and Plant Breeding, Isfahan (Khorasgan) Branch, Islamic Azad University, P.O.Box:81595-158, Isfahan, Iran, e-mail:dragolparvar@gmail.com

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Published in „GENETIKA“ Vol. 48, No. 3(2016), pp. 1111-1119

Serbia

UDC 575

DOI: 10.2298/GENSR1603111W

Original scientific paper

DIVERSITY OF METHANE-PRODUCING MICROBIAL COMMUNITY STRUCTURE WITH 16S-V3 rDNA PCR-DGGE

Wang NA¹, Wang HONG², Hui-Ying LIU¹, Muhammad IRFAN³, Shi-Wei NIU¹, Yang TAO²^＊

¹Institute of Plant nutrients and Environmental Resouces, Liaoning Academy of Agricultural Sciences, Liaoning 110161,China

²Center of Microbial Engineering, Liaoning Academy of Agricultural Sciences, Shenyang, Liaoning 110161, China

³Bioscience & Biotechnology College, Shenyang Agricultural University, Shenyang, Liaoning China

Abstract

Wang N., W.Hong, H.-Y. Liu, M. Irfan, S.-W. Niu, Y. Tao (2016): Diversity of methane-producing microbial community structure with 16S-V3 RDNA PCR-DGGE.- Genetika, Vol 48, No.3, 1111 - 1119.

The method of 16S-V3 rDNA PCR-DGGE was utilized to study the diversity of methane-producing microbial community structure under different methane-producing microbial community structure. Results showed that community structure of the methanogens under different environmental condition was significantly different. The number of bands about community under extreme condition was less than normal conditions, but the main bands were obvious. The sequence analysis showed that the predominant strains mainly were uncultured bacteria that could not be identified in the level of species .Z2 was Clostridia, Z8 was Clostridiales, D5 was Eubacteriaceae, and D8 was Flavobacteriaceae. This study laid some extent foundation for future research about establishing a methane-producing bacterial system with high gas yield in winter.

Keywords: community structure, PCR-DGGE, uncultured bacteria

Corresponding author: Yang Tao,Center of Microbial Engineering, Liaoning Academy of Agricultural Sciences, Shenyang, Liaoning 11016 China

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