GENETIKA, Vol

GENETIKA, Vol. 48, No. 1 (2016)

Fuad NUR AZIS and dan Kuntoro BOGA ANDRI

MORPHOLOGICAL DIVERSITY IN FOURTEEN CULTIVARS TIRON VILLAGE, KEDIRI, INDONESIA [Abstract] [Full text]

Ahmed M. EL-SHEHAWI, Saad A. AL-OTAIBI, and Ehab M. AZAB

DEFENSIN GENE EXPRESSION IN SOME PLANT SOURCES OF TAIF [Abstract] [Full text]

Mashhid HENAREH, Atilla DURSUN, Babak ABDOLLAHI MANDOULAKANI, Kamil HALİLOĞLU

ASSESSMENT OF GENETIC DIVERSITY IN TOMATO LANDRACES USING ISSR MARKERS [Abstract] [Full text]

Jelena P. JOVICIC-PETROVIC, Ivana M. STANKOVIC, Aleksandra R. BULAJIC, Branka B. KRSTIC, Dragan D. KIKOVIC, Vera B. RAICEVICFILAMENTOUS FUNGI ISOLATED FROM GRAPE MARC AS ANTAGONISTS OF Botrytis cinerea [Abstract] [Full text]

Almira SOFTIĆ, Katica VELIJA, Jasmin RAMIĆ, Kasim BAJROVIĆ, Gabrijela RADOSAVLJEVIĆ, Lejla LASIĆ, Belma KALAMUJIĆ, Vedad ŠAKIĆ, Naris POJSKIĆ

MICROSATELITE DIVERSITY OF BOSNIAN-HERZEGOVINIAN-CROATIAN SHEPHERD DOG TORNJAK [Abstract] [Full text]

Marijana RAKONJAC, Goran CUTURILO, Milena STEVANOVIC, Ida JOVANOVIC, Ljiljana JELICIC DOBRIJEVIC, Marija MIJOVIC, Danijela DRAKULIC

SPEECH AND LANGUAGE ABILITIES OF CHILDREN WITH THE FAMILIAL FORM OF 22Q11.2 DELETION SYNDROME [Abstract] [Full text]

Jafar AHMADI, Behroz VAEZI, Alireza POUR-ABOUGHADAREH

ANALYSIS OF VARIABILITY, HERITABILITY, AND INTERRELATIONSHIPS AMONG GRAIN YIELD AND RELATED CHARACTERS IN BARLEY ADVANCED LINES [Abstract] [Full text]

Fatih S. BERIS, Necla PEHLIVAN, Melike KAC, Ayhan HAZNEDAR, Fatih COŞKUN, Cemal SANDALLI

VALUATION OF GENETIC DIVERSITY OF CULTIVATED TEA CLONES (Camellia sinensis (L.) KUNTZE) IN THE EASTERN BLACK SEA COAST BY INTER-SIMPLE SEQUENCE REPEATS (ISSRS) [Abstract] [Full text]

Vladimir TRUKHACHEV, Valery BELYAEV, Andrey KVOCHKO, Alexander KULICHENKO, Dmitry KOVALEV, Sergey PISARENKO, Anna VOLYNKINA, Marina SELIONOVA, Magomet AYBAZOV, Svetlana SHUMAENKO, Arslan OMAROV, Tatyana MAMONTOVA, Olesya YATSYK, Alexander KRIVORUCHKO, Milan P. P ETROVIC, Vlada ANTELIC, Violeta PETROVIC CARO

MEF2B GENE SNP MARKERS OF MEAT PRODUCTIVITY IN SEVEROKAVKAZSKAYA SHEEP BREED [Abstract] [Full text]

Nebojša MOMIROVIĆ, Zoran BROĆIĆ, Rade STANISAVLJEVIĆ, Ratibor ŠTRBANOVIĆ, Goran GVOZDEN, Aleksandra STANOJKOVIĆ-SEBIĆ, Dobrivoj POŠTIĆ

VARIABILITY OF DUTCH POTATO VARIETIES UNDER VARIOUS AGROECOLOGICAL CONDITIONS IN SERBIA [Abstract] [Full text]

Reyhaneh SEYYED-NAZARI, Mortaza GHADIMZADEH, Reza DARVISHZADEH, Seyyed Reza ALAVI

DIALLEL ANALYSIS FOR ESTIMATION OF GENETIC PARAMETERS IN ORIENTAL TOBACCO GENOTYPES [Abstract] [Full text]

Ezatollah FARSHADFAR, Reza AMIRI

ASSESSMENT OF GENETIC DIVERSITY AND ESTIMATION OF GENETIC PARAMETERS FOR REMOBILIZATION RELATED TRAITS OF WHEAT UNDER DROUGHT CONDITIONS [Abstract] [Full text]

Saidin saclain, Md Abdul latif, Babul bala, Mithun mallik, Md. Shahidul islam

GENETIC DIVERSITY ANALYSIS OF TROPICAL SUGAR BEET (Beta vulgaris L.) VARIETIES IN BANGLADESH USING RAPD MARKERS [Abstract] [Full text]

Jovan PAVLOV, Nenad DELIĆ, Tomislav ŽIVANOVIĆ, Danijela RISTIĆ, Zoran ČAMDŽIJA, Milan STEVANOVIĆ, Miodrag Tolimi

RELATIONSHIP BETWEEN GENETIC DISTANCE, SPECIFIC COMBINING ABILITIES AND HETEROSIS IN MAIZE (Zea mays L.) [Abstract] [Full text]

Yuksel kaya, Ramazan ayranci

BREEDING BARLEY FOR QUALITY IN TURKEY [Abstract] [Full text]

Tanja VASIĆ,Vesna KRNJAJA, Darko JEVREMOVIĆ, Slavica STANKOVIĆ, Dragan Terzić, Jasmina MILENKOVIĆ, Jordan MARKOVIĆ

VEGETATIVE COMPATIBILITY AND RFLP ANALYSIS OF Colletotrichum destructivum ISOLATES FROM ALFALFA AND RED CLOVER [Abstract] [Full text]

A.I ateya, Y.Y el-seady, S.M. atwa, B.H. merghani and N.A. sayed

NOVEL SINGLE NUCLEOTIDE POLYMORPHISMS IN LACTOFERRIN GENE AND THEIR ASSOCIATION WITH MASTITIS SUSCEPTIBILITY IN HOLSTEIN CATTLE [Abstract] [Full text]

Jana ŽIAROVSKÁ, Michal ZÁHORSKÝ, Andrea HRICOVÁ

Prosystemin identification in Amaranthus cruentus and A. hypochondriacus x hybridus based on data mining and sequence alignment [Abstract] [Full text]

Srbislav DENČIĆ, Ron DEPAUW, Vojislava MOMČILOVIĆ and Vladimir AĆIN
COMPARISON OF SIMILARITY COEFFICIENTS USED FOR CLUSTER ANALYSIS BASED ON SSR MARKERS IN SISTER LINE WHEAT CULTIVARS [Abstract] [Full text]

R. EL-BAKATOUSH, A. ELFRAMAWY

DIVERSITY IN GROWTH AND EXPRESSION PATTERN OF POHKT1 AND POVHA TRANSPORTER GENES UNDER NaCl STRESS IN Portulaca oleracea TAXA [Abstract] [Full text]

Liwei DONG, Ning LI, Xiaobing LU, Slaven PRODANOVIC , Yanhao XU,Wenying ZHANG , Yueming YAN

QUALITY PROPERTIES AND EXPRESSION PROFILING OF PROTEIN DISULFIDE ISOMERASE GENES DURING GRAIN DEVELOPMENT OF THREE SPRING WHEAT NEAR ISOGENIC LINES [Abstract] [Full text]

n. chandrakanth, s.m. moorthy, m. rekha and v. sivaprasad

STABILITY AND PATH ANALYSIS FOR YIELD AND RELATED TRAITS IN SILKWORM, (Bombyx mori L.) REARED UNDER STRESS CONDITIONS [Abstract] [Full text]

Renata ILIČIĆ, Jelica BALAŽ, Vera STOJŠIN, Dragana JOŠIĆ

CHARACTERIZATION OF Pseudomonas syringae PATHOVARS FROM DIFFERENT SWEET CHERRY CULTIVARS BY RAPD ANALYSIS [Abstract] [Full text]

Luka ANDRIĆ, Vlado KOVAČEVIĆ, Imre KADAR, Antun JAMBROVIĆ, Hrvoje PLAVŠIĆ and Domagoj ŠIMIĆ

GENOTYPIC EFFECTS ON BORON CONCENTRATIONS AND RESPONSE ON BORON FERTILIZATION IN MAIZE INBRED LINES [Abstract] [Full text]

Noha S. khalifa, Maher M. shehata, Ahmed F. abodoma, Abdullah F. al-kumbezy

MOLECULAR ANALYSIS OF COMMERCIAL DATE PALM CULTIVARS IN LYBIA USING ISSR AND SRAP PCR- BASED MARKERS [Abstract] [Full text]

Sohail Ahmad JAN, Zabta Khan SHINWARI, Malik Ashiq RABBANI

MORPHO- BIOCHEMICAL EVALUATION OF Brassica rapa SUB-SPECIES FOR SALT TOLERANCE [Abstract] [Full text]

Dario DANOJEVIĆ, Živko ĆURČIĆ, Nevena NAGL, Ksenija TAŠKI-AJDUKOVIĆ, Jan BOĆANSK

EVALUATION OF SUGAR BEET GENOTYPES FOR ROOT TRAITS BY PRINCIPAL COMPONENT ANALYSIS AND CLUSTER ANALYSIS [Abstract] [Full text]

Ivana bakić, Vera rakonjac, Dragan nikolić, Milica fotirić-akšić, Slavica čolić, Aleksandar radović

CHARACTERIZATION OF THE VINEYARD BYOTYP COLLECTION OF PEACH AS STEP IN PREBREEDING PROCESS [Abstract] [Full text]

Mahmoud A. ALOMARI, Omar F. KHABOUR,,Asmaa A. ABU OBAID

THE ASSOCIATION BETWEEN ADIPONECTIN AND ADIPOQ GENE POLYMORPHISMS WITH OBESITY AMONG YOUNG JORDANIAN WOMEN [Abstract] [Full text]

Gordana PETROVIĆ, Dušica JOVIČIĆ, Zorica NIKOLIĆ, Gordana TAMINDŽIĆ, Maja IGNJATOV, Dragana MILOŠEVIĆ, Branko MILOŠEVIĆ

COMPARATIVE STUDY OF DROUGHT AND SALT STRESS EFFECTS ON GERMINATION AND SEEDLING GROWTH OF PEA [Abstract] [Full text]

Ayşe Nilgün ATAY, Şerif ÖZONGUN, Turgay SEYMEN, Alamettin BAYAV, Ersin ATAY

Variability and heritability of floral development in apple full-sib offsprings [Abstract] [Full text]

Dubravka VEJNOVIC, Vera Milic, Tatjana DAMNJANOVIC, Nela MAKSIMOVIC, Vera BUNJEVACKI, Ljiljana LUKOVIC, Ivana NOVAKOVIC, Maja KRAJINOVIC, Nemanja DAMJANOV, Goran RADUNOVIC, Sofija PAVKOVIC-LUCIC and Biljana JEKIC

ANALYSIS OF ASSOCIATION BETWEEN POLYMORPHISMS OF MTHFR, MTHFD1 AND RFC1 GENES AND EFFICACY AND TOXICITY OF METHOTREXATE IN RHEUMATOID ARTHRITIS PATIENTS [Abstract] [Full text]

Srdja JANKOVIĆ, Irena MARJANOVIĆ, Nataša Tošić, Nikola KOTUR, Lidija DOKMANOVIĆ, Dejan ŠKORIC, Nada KRSTOVSKI, Jelena LAZIĆ, Predrag RODIĆ, Sonja PAVLOVIĆ,Dragana JANIĆ

WILMS TUMOR (WT)1 GENE EXPRESSION IN CHILDREN WITH ACUTE LEUKEMIA IN SERBIA [Abstract] [Full text]

Khadija AALIYA, Zahida QAMAR, Idrees AHMAD NASIR, Qurban ALI, Abdul MUNIM FAROOQ and Tayyab HUSNAIN

TRANSFORMATION, EVALUATION OF GTGENE AND MULTIVARIATE GENETIC ANALYSIS FOR MORPHO-PHYSIOLOGICAL AND YIELD ATTRIBUTING TRAITS IN Zea mays [Abstract] [Full text] [ Supplementary material 1] [Supplementary material 2]

Published in „GENETIKA“ Vol. 48, No. 1 (2016), pp. 1-8

UDC 575.630
DOI: 10.2298/GENSR1601001N

Orginal scientific paper

MORPHOLOGICAL DIVERSITY IN FOURTEEN CULTIVARS TIRON VILLAGE, KEDIRI, INDONESIA

Fuad NUR AZIS* and dan Kuntoro BOGA ANDRI

Jl. Raya Karangploso KM.4 Malang 65152, Jawa Timur Indonesia

Abstract

Nur Azis F. and dan K. Boga Andri (2016): Morphological diversity in fourteen cultivars Tiron village, Kediri, Indonesia. - Genetika, vol 48, no. 1, 1 -8.

Tiron village, Kediri is one of central mangoes in Indonesia. Many cultivar of mangoes encountered at this location. This study aims to look at the diversity of mango cultivars in the Tiron Village, Kediri, Indonesia. Mangoes diversity is based on qualitative and quantitative character of each cultivar. The diversity among cultivar indicated by the standard deviation and variance in the eleven quantitative characters of mango. Mango cultivars categorized by phylogeny morphological characters. The method used for phylogeny analysis is an UPGMA method (unweighted pair group method with arithmetic mean). Phylogenic analysis is based by the qualitative character of the plant. The results showed there were fourteen cultivars of mango in the village of Tiron Kediri have high diversity. Fourteen mango cultivars were categorized four groups. Based on a qualitative character, there are four classes of mango. The first group is the Katul, Podang Urang, and Podang Lumut. The second group is the Gadung, Jaran, Madu, Endog, Pakel, Dodonilo, Ireng, Lanang and Cantek. Santok Kapur into groups to form groups of three and Kopyor fourth. The high diversity in the village mango Tiron Kediri potential for resource in situ germplasm.

Key words: Manggo, Phylogenic analysis, UPGMA

Corresponding author: Fuad Nur Azis, BPTP Jawa Timur Jl. Raya Karangploso KM.4 Malang 65152, Jawa Timur Indonesia. Phone 0341 494052, 485055 Fax 0341-471255. email: fn_azis@litbang.pertanian.go.id or fn_azis@yahoo.com.

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Published in „GENETIKA“ Vol. 48, No. 1 (2016), pp. 9 -24

Serbia

UDC 575.630

DOI: 10.2298/GENSR1600209E

Original scientific paper

DEFENSIN GENE EXPRESSION IN SOME PLANT SOURCES OF TAIF

Ahmed M. EL-SHEHAWI^{1, 2,†}, Saad A. AL-OTAIBI¹, and Ehab M. AZAB^{1, 3}

¹Department of Biotechnology, Faculty of Science, Taif University, Taif, Saudi Arabia

²Department of Genetics, Alexandria University, Alexandria, Egypt

³Department of Botany, Faculty of Science, Zagazig University, Zagazig, Egypt

Abstract

el-shehawiA.M., S.A. al-otaibi, and E. m. azab (2016): Defensin gene expression in some plant sources of Taif - Genetika, Vol 48, No.1, 9-24.

Plant defensins are promising future strategy as antimicrobial agents; however the number of characterized plant defensins is very low. We isolated and studied the expression of plant defensins in six plants from Taif region. Using RT-PCR and two pairs of common as well as three pairs of specific primers, the defensin gene expression was analyzed. Plants showed differences in defensin gene expression in floral buds and leaves, though floral buds represented higher gene expression. The amino acid sequence of the six isolated cDNA sequences showed high similarity with other defensin accessions from the nucleotide database, especially in the cysteine rich motif. Phylogenetic analysis revealed that the isolated sequences share the common features of plant defensins, especially the 8 conserved cysteines. The results of this study help to detect some valuable Saudi plant sources for the development of natural peptides as a replacement for chemical antibiotics.

Keywords: Plant, Defensin, gene expression, Antimicrobial peptides, Phylogenetic analysis.

Corresponding author: Ahmed M. el-shehawi, Department of Biotechnology, Faculty of Science, Taif University, Taif, Saudi Arabia,,Email: elshehawi@hotmail.com

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Published in „GENETIKA“ Vol. 48, No. 1 (2016), pp.25- 35

Serbia

UDC 575.630

DOI: 10.2298/GENSR1601025H

Original scientific paper

ASSESSMENT OF GENETIC DIVERSITY IN TOMATO LANDRACES USING ISSR MARKERS

Mashhid HENAREH^1*, Atilla DURSUN², Babak ABDOLLAHI MANDOULAKANI³,

Kamil HALİLOĞLU⁴

¹West Azerbaijan Agricultural and Natural Resources Research Center, Urmia, Iran.

²Department of Horticulture, Faculty of Agriculture, Ataturk University,Erzurum, Turkey.

³Department of Plant Breeding and Biotechnology, Faculty of Agriculture,Urmia University, Urmia, Iran.

⁴Department of Field Crops, Faculty of Agriculture, Ataturk University, Erzurum, Turkey.

Abstract

Henareh M., A. Dursun, B. A. Mandoulakani, K. Haliloğlu (2016): Assessment of genetic diversity in tomato landraces using ISSR markers.- Genetika, Vol 48, No. 1, 25 - 35.

Tomato is one of the most economically important vegetable crops in many parts of the world. Turkey and Iran are the main producers of tomatoes in the world. The objective of this study was to assess the genetic variation of 93 tomato landraces from East Anatolian region of Turkey and North-West of Iran, along with three commercial cultivars using 14 ISSR primers. The percentage of polymorphic loci (PPL) for all primers was 100%. The mean of expected heterozygosity (He) for the primers varied from 0.153 (UBC808) to 0.30 (UBC848). The dendrogram placed the landraces and commercial cultivars into nine groups. The genotypes originating from the same region, often located in the same group or two adjacent groups. The highest likelihood of the data was obtained when population were located into 2 sub-populations (K = 2). These sub-populations had Fst value of 0.16 and 0.21.

Key words: ISSR, genetic variation, Shannon’s information index, Solanum lycopersicum.

Corresponding author: M. Henareh, West Azerbaijan Agricultural and Natural Resources Research Center, Urmia, Iran. E-mail: mashhid_henareh@yahoo.com Phone:+989144407629. Fax:+98 44 2622220

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Published in „GENETIKA“ Vol. 48, No1 (2016), pp. 37 -48

Serbia

UDC 575.630
DOI: 10.2298/GENSR1601037J
Original scientific paper

FILAMENTOUS FUNGI ISOLATED FROM GRAPE MARC AS ANTAGONISTS

OF Botrytis cinerea

Jelena P. JOVICIC-PETROVIC¹, Ivana M. STANKOVIC¹, Aleksandra R. BULAJIC¹,

Branka B. KRSTIC¹, Dragan D. KIKOVIC², Vera B. RAICEVIC¹

¹Faculty of Agriculture, University of Belgrade, Belgrade, Serbia

²Faculty of Natural Sciences, Kosovska Mitrovica, Serbia

Abstract

Jovicic-Petrovic P. J., I. M. Stankovic, A. R. Bulajic, B. B. Krstic, D. D. Kikovic, V. B. Raicevic (2016): Filamentous fungi isolated from grape marc as antagonists of Botrytis cinerea.-Genetika, vol 48 , no.1, 37-48.

In this paper we report on the isolation and identification of three filamentous fungi from grape marc, and antifungal effect of their cell-free culture filtrates on the growth of Botrytis cinerea, causal agent of gray mold. Grape marc is a waste material that has been used as soil amendment in sustainable agriculture. Isolates originating from grape marc were identified on the basis of morphological features and internal transcribed spacer rDNA or β-tubulin gene sequencing. The presence of three different species, Penicillium paneum, Penicillium chrysogenum and Aspergillus fumigatus has been detected expressing different effect on the growth of B. cinerea.

The effect of crude culture filtrates of selected fungi on B. cinerea growth was tested. Heat sensitivity of the established inhibition effect was examined by autoclaving the crude culture filtrate prior to testing. Additional aim was to determine whether antifungal effect was influenced by previous exposure to B. cinerea in dual liquid cultures. Crude culture filtrate of A. fumigatus K16/2 showed the lowest suppression of B. cinerea growth. A maximal percentage inhibition achieved within the study was 38.2%, 39.8% and 23.8 for crude filtrates of P. paneum K7/1, P. chrysogenum K11/1 and A. fumigatus K16/2, respectively. Presence of B. cinerea in dual liquid culture induced significant increase in antifungal capacity of the culture filtrates in comparison to pure culture filtrates of the chosen isolates. The antifungal activity of all of the isolates’ culture filtrates retained after heat treatment suggesting the presence of some thermostable antifungal metabolites. The results indicate the complexity and specificity of the interaction between filamentous fungi and B. cinerea. Grape marc is a good source for isolation od B. cinerea fungal antagonists and their antifungal metabolites. Specificity of fungal-fungal interactions suggests that further research on the antagonistic mechanisms and factors affecting them should be studied separately for each pair of antagonists.

Key words: Aspergillus, Botrytis cinerea, culture filtrate, growth inhibition, Penicillium

Corresponding author: Jelena Jovicic Petrovic,Postal address: Faculty of Agriculture, Nemanjina 6, Zemun, Serbia,Tel: +381 11 2615315 ext. 162,Fax: +381 11 2193659, E-mail: jelenap@agrif.bg.ac.rs

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Published in „GENETIKA“ Vol. 48, No.1 (2016), pp. 49 -56

Serbia

UDC 575
DOI: 10.2298/GENSR1601049S
Original scientific paper

MICROSATELITE DIVERSITY OF BOSNIAN-HERZEGOVINIAN-CROATIAN SHEPHERD DOG TORNJAK

Almira softić^*, Katica velija^*, Jasmin ramić^**, Kasim bajrović ^**, Gabrijela radosavljević^**, Lejla lasić^**, Belma kalamujić^**, Vedad šakić^*, Naris pojskić^**

^*University of Sarajevo, Veterinary Faculty, Department of Zootechnics and Poultry Production, Bosnia and Herzegovina

^** University of Sarajevo, Institute for Genetic Engineering and Biotechnology, Bosnia and Herzegovina

Abstract

softić A., K. velija J. ramić, K. bajrović, G. radosavljević, L. lasić., B. kalamujić, V. šakić, N. pojskić (2016): Microsatelite diversity of Bosnian-Herzegovinian-Croatian shepherd dog Tornjak.- Genetika, Vol 48, No.13,49 -56.

This paper presents the first estimation of polymorphism of the Bosnian-Herzegovinian-Croatian Shepherd dog Tornjak in Bosnia and Herzegovina using 10 microsatellite loci, which are an integral part of StockMarks^® for Canine Genotyping Kit (Applied Biosystems, Foster City, CA, USA). Ten microsatellite loci used in this study are appropriate for assessing the genetic diversity for this breed. Measures of genetic diversity were estimated based on allelic and genotypic calculations, observed (H_O) and expected (H_E) heterozygosities, deviations from Hardy-Weinberg equilibrium and polymorphism information content (PIC). The lowest genetic diversity was estimated for locus PEZ20, and the highest for PEZ6 locus. Observed and expected mean heterozygosities were 0.7261 and 0.7392, respectively. Statistically significant deviation (p<0.05) from Hardy-Weinberg equilibrium was found for PEZ1, PEZ12, PEZ3 and PEZ6 loci. The PIC values suggested that all markers (100%) are very informative (PIC > 0.5) in terms of their suitability for genetic diversity studies. When all observed parameters are taken into account (observed and expected heterozygosities, PIC, number of detective and effective alleles, number of detected and maximum possible genotypes, major allele frequency and major allele frequency index), we can conclude that PEZ6 locus shows the highest genetic diversity while PEZ3 displays the lowest. However, assuming values of observed and expected heterozygosities, as well as PIC, we consider loci PEZ20 to be the least diverse, but this locus has more effective alleles and more genotypes present than PEZ3. These preliminary results are the first genetic diversity survey of the Bosnian-Herzegovinian-Croatian Shepherd dog Tornjak in Bosnia and Herzegovina and could be useful to the dog breeders in designing and managing breeding strategies. Summarizing the information above, we can conclude that the population of the Bosnian-Herzegovinian-Croatian Shepherd dog Tornjak from B&H is not affected by substantial loss of genetic diversity. Results of our study indicate presence of reasonably high level of genetic variability and lead to a better understanding of this dog breed.

Key words: alleles, frequencies, microsatellites, genetic diversity, Bosnian-Herzegovinian-Croatian Shepherd dog Tornjak.

Corresponding author: Naris Pojskić,Institute for Genetic Engineering and Biotechnology, University of Sarajevo,Zmaja od Bosne 8, 71000 Sarajevo, Bosnia and Herzegovina,Phone:+38733220926, fax:+38733442891,E mail: naris.pojskic@ingeb.unsa.ba

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Published in „GENETIKA“ Vol. 48, No. 1 (2016), pp. 57 -72

Serbia

UDC 575
DOI: 10.2298/GENSR1601057R
Original scientific paper

SPEECH AND LANGUAGE ABILITIES OF CHILDREN WITH THE FAMILIAL FORM OF 22Q11.2 DELETION SYNDROME

Marijana RAKONJAC^a,b,*, Goran CUTURILO^c,d, Milena STEVANOVIC^e, Ida JOVANOVIC^C,^d,,

Ljiljana JELICIC DOBRIJEVIC^A,b, Marija MIJOVIC^d, Danijela DRAKULIC^e

^aInstitute for experimental phonetics and speech pathology, Belgrade, Serbia

^bLife activities advancement center, Belgrade, Serbia

^cFaculty of Medicine, University of Belgrade, Belgrade, Serbia

^dUniversity Children’s Hospital, Belgrade, Serbia

^eInstitute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia

Abstract

Rakonjac M., G. Cuturilo, M. Stevanovic, I.Jovanovic^,, Lj. Jelicic Dobrijevic, M. Mijovic, D. Drakulic (2016): Speech and language abilities of children with the familial form of 22q11.2 deletion syndrome- Genetika, Vol 48, No. 1, 57-72.

The 22q11.2 Deletion Syndrome (22q11.2DS), which encompasses Shprintzen syndrome, DiGeorge and velocardiofacial syndrome, is the most common microdeletion syndrome in humans with an estimated incidence of approximately 1/4000 per live births. After Down syndrome, it is the second most common genetic syndrome associated with congenital heart malformations. The mode of inheritance of the 22q11.2DS is autosomal dominant. In approximately 72 - 94% of the cases the deletion has occurred de novo, while in 6 to 28% of patients deletion was inherited from a parent. As a part of a multidisciplinary study we examined the speech and language abilities of members of two families with inherited form of 22q11.2DS. The presence of 22q11.2 microdeletion was revealed by fluorescence in situ hybridization (FISH) and/or multiplex ligation-dependent probe amplification (MLPA). In one family we detected 1.5 Mb 22q11.2 microdeletion, while in the other family we found 3Mb microdeletion. Patients from both families showed delays in cognitive, socio-emotional, speech and language development. Furthermore, we found considerable variability in the phenotypic characteristics of 22q11.2DS and the degree of speech-language pathology not only between different families with 22q11.2 deletion, but also among members of the same family. In addition, we detected no correlation between the phenotype and the size of 22q11.2 microdeletion.

Key words: 22q11.2DS, speech and language, mode of inheritance, size of deletion

Corresponding author: Marijana Rakonjac,Institute for experimental phonetics and speech pathology,Life activities advancement center,Jovanova 35,11000 Belgrade,Serbia,Phone: +381 11 3208 500,E-mail: masadanicic@gmail.com

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Published in „GENETIKA“ Vol. 48, No.1 (2016), pp.73 -85

Serbia

UDC 575.633.11
DOI: 10.2298/GENSR1601073A

Original scientific paper

ANALYSIS OF VARIABILITY, HERITABILITY, AND INTERRELATIONSHIPS AMONG GRAIN YIELD AND RELATED CHARACTERS IN BARLEY ADVANCED LINES

Jafar AHMADI¹, Behroz VAEZI², Alireza POUR-ABOUGHADAREH^1*

¹Department of Crop production and Breeding, Imam Khomeini International University, Qazvin, Iran

²Faculty member of Gachsaran Agricultural Research Station, Gachsaran, Iran.

Abstract

Ahmadi J., B.Vaezi, Alireza Pour-Aboughadareh (2016): Analysis of variability, heritability, and interrelationships among grain yield and related characters in barley advanced lines--Genetika, Vol 48, No. 1, 73-85.

The main goal of this study was to improve pure lines for low input agricultural areas of the semi-arid region of Iran and similar environmental conditions. Forty barley pure lines provided from ICARDA along with three check cultivars were evaluated under rain-fed conditions in a semi-arid region of Iran during two years (2011-2013). The experiments were conducted in a randomized complete block design with four replications to estimate genetic variation and heritability for agro-morphological traits. The highest values of phenotypic and genotypic coefficients of variation were observed for the number of grains per spike followed by peduncle length, early vigor and grain yield. The broad heritability estimates ranged from 24% for grain yield to 96% for the number of grains per spike. The highest and lowest expected genetic advance, observed for days to physiological maturity and the number of grains per spike, respectively. Results of stepwise regression and path analysis showed that hectoliter grain weight and thousand grain weight exhibited the highest direct effects on grain yield, respectively. Comparisons between the pure lines and check cultivars indicated that out of 40 pure lines, 27 lines had a higher grain yield. Also, the pure lines No. 29, 13, 9 and 33 were identified as the superior lines for semi-arid environmental conditions. Our results indicate that check cultivars could be improved by selecting for pure lines with taller peduncle and the number of grains per spike, but with heavier grains. Therefore, these lines can be used as genetic material to broaden the genetic basis of barley breeding programs all over the world.

Key words: Agronomic traits, correlation, genetic variation, heritability, path analysis, yield performance.

Corresponding author: Alireza Pour-Aboughadareh, Department of Crop Production and Breeding, Imam Khomeini International University, Qazvin, Iran. Email: a.poraboghadareh@edu.ikiu.ac.ir.com

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Published in „GENETIKA“ Vol. 48, No.1 (2016), pp.87-96

Serbia

UDC 575
DOI: 10.2298/GENSR1601087B
Original scientific paper

EVALUATION OF GENETIC DIVERSITY OF CULTIVATED TEA CLONES (Camellia sinensis (L.) KUNTZE) IN THE EASTERN BLACK SEA COAST BY INTER-SIMPLE SEQUENCE REPEATS (ISSRS)

Fatih S. BERIS¹, Necla PEHLIVAN¹, Melike KAC¹, Ayhan HAZNEDAR², Fatih COŞKUN³, Cemal SANDALLI^1*

¹Department of Biology, Faculty of Arts & Sciences, Recep Tayyip Erdogan University, Rize, Turkey

²Ministry of Agriculture, Ataturk Tea and Horticulture Research Institute, Rize Turkey

³Department of Biology, College of Arts & Sciences, Balikesir University, Cagis Campus, Balikesir, Turkey

Abstract

Beris S. F., N. Pehlivan, M. Kac, A. Haznedar, F. Coşkun, C.Sandalli (2016): Evaluation of genetic diversity of cultivated tea clones (Camellia sinensis (l.) kuntze) in the Eastern black sea coast by inter-simple sequence repeats (ISSRS)- Genetika, Vol 48, No. 1, 87-96.

Tea is the most globally consumed drink after spring water and an important breeding plant with high economical value in Turkey. In half a century, various kinds of tea cultivars have been bred in Turkey to improve the quality and yield of tea plants. Since tea reproduces sexually, tea fields vary in quality. Thus, determining the genetic diversity and relationship of the plants to support breeding and cultivation is important. In this study we aimed to determine the genetic diversity of tea cultivars breeding in the Eastern Black Sea coast of Turkey and the genetic relationship between them, to verify whether the qualitative morphological designations of the clones are genetically true by the ISSR markers. Herein, the genetic diversity and relationships of 18 Turkish tea cultivars were determined using 15 ISSR markers with sizes ranging from 250 to 3000 base pairs. The similarity indices among these cultivars were between 0.456 and 0.743. Based on cluster analysis using UPGMA, some of tea cultivars originating from the same geographical position were found to be clustered closely. Our data provide valuable information and a useful basis to assist selection and cloning experiments of tea cultivars and also help farmers to find elite parental clones for tea breeding in the Eastern Black Sea coast of Turkey.

Key words: Genetic polymorphism, PCR based markers, Tea breeding, Turkish tea cultivars, UPGMA clustering

Corresponding author: Cemal sandalli,Department of Biology, Faculty of Arts & Sciences, Recep Tayyip Erdogan University, 53100, Rize, Turkey,Tel No: +90 464 223 61 26 Ext: 1828.Fax No: +90 464 223 4019, cemal.sandalli@erdogan.edu.tr

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Published in „GENETIKA“ Vol. 48, No.1 (2016), pp. 97-108

Serbia

UDC 575

DOI: 10.2298/GENSR1601097T
Original scientific paper

MEF2B GENE SNP MARKERS OF MEAT PRODUCTIVITY IN

SEVEROKAVKAZSKAYA SHEEP BREED

Vladimir TRUKHACHEV¹, Valery BELYAEV¹, Andrey KVOCHKO¹, Alexander KULICHENKO², Dmitry KOVALEV², Sergey PISARENKO², Anna VOLYNKINA², Marina SELIONOVA³, Magomet AYBAZOV³, Svetlana SHUMAENKO³, Arslan OMAROV³, Tatyana MAMONTOVA³, Olesya YATSYK¹, Alexander KRIVORUCHKO¹, Milan P. P ETROVIC⁴,

Vlada ANTELIC ⁴, Violeta PETROVIC CARO⁴

¹Faculty of Veterinary Medicine, Stavropol State Agrarian University, Stavropol, Russian Federation

²Stavropol Research Anti-plague Institute, Stavropol, Russian Federation

³All-Russian Research Institute of Sheep and Goat Breeding, Stavropol, Russian Federation

⁴Institute for Animal Husbandry, Belgrade, Republic of Serbia

Abstract

Trukhachev V., V. Belyaev, A.Kvochko, A. Kulichenko, D. Kovalev, S.Pisarenko, A. Volynkina, M. Selionova, M. Aybazov, S. Shumaenko, A.Omarov, T. Mamontova, O. Yatsyk, A. Krivoruchko, M P. Petrovic, V.Pantelic, V. Petrovic Caro (2016): MEF2B gene SNP markers of meat productivity in Severokavkazskaya sheep breed.- Genetika, Vol 48, No. 1, 97-108.

One of the new promising candidate genes defining productive qualities of sheep is MEF2B. Protein from the MEF2 group encoded by it affects the production of myostatin and the expression of the genes responsible for the growth of skeletal muscle fibers. Thus, the knowledge of the MEF2B gene structure is important for genomic selection. We have studied the structure of the MEF2B gene at sheep of Severokavkazskaya breed bred in Russia. To detect alleles we use NimbleGen sequencing technology by Roche (USA). As a result, it was revealed 14 single nucleotide polymorphisms (SNP) at the given breed. The discovered SNPare located in not coding areas. From them 7 polymorphisms are in the area of 5’ upstream gene in loci: c.-1713, c.-1319, c.-839, c.-321, c.-246, c.-161, c.-3; 6 polymorphisms are in introns, loci: c.55-51, c.258+312, c.258+380, c.259-52, c.452+95, c.452+103, 1 SNP is in 3’ downstream gene, c.*252. Two of the identified SNPs are significantly connected with high indices of meat productivity: c.55-51 and c.259-52. At the same time it was not possible to find out the impact on productivity of c.-1713 polymorphism. Our investigation is a base of next research of affection of different MEF2B gene alleles on meat quality and can be used to prepare PCR test-system for genomic selection.

Key words: genomic election, MEF2B, sequence, sheep, SNP

Corresponding author: Alexander Krivoruchko, Faculty of Veterinary medicine, Stavropol State Agrarian University, Stavropol, Russian Federation, 355017, Zootehnichesky, 12, Tel:+79188814327, Fax:+78652286738, E-mail: rcvm@yandex.ru

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Published in „GENETIKA“ Vol. 48, No. 1 (2016), pp. 109 - 124

Serbia

UDC 575.633
DOI: 10.2298/GENSR1601109M
Original scientific paper

VARIABILITY OF DUTCH POTATO VARIETIES UNDER VARIOUS AGROECOLOGICAL CONDITIONS IN SERBIA

Nebojša MOMIROVIĆ¹, Zoran BROĆIĆ¹, Rade STANISAVLJEVIĆ², Ratibor ŠTRBANOVIĆ², Goran GVOZDEN³, Aleksandra STANOJKOVIĆ-SEBIĆ⁴, Dobrivoj POŠTIĆ²

¹ Faculty of Agriculture, Belgrade, Serbia

²Institute for Plant Protection and Environment, Belgrade, Serbia

³ Golić trejd d.o.o., Gradiška, Republika Srpska

⁴Institute of Soil Science, Belgrade, Serbia

Abstract

Momirović N., Z. Broćić, R. Stanisavljević, R. Štrbanović, G. Gvozden, A. Stanojković-Sebić, . Poštić (2016): Variability of Dutch potato varieties under various agroecological conditions in Serbia- Genetika, Vol 48, No. 1, 109-124.

The study presents results of a three-year experiment of variability of different Dutch potato varieties in Serbia: Adora and Cleopatra (early), Innovator and Frisia (medium-early) and Desiree and Kondor (medium-late). The research was conducted during 2008, 2009 and 2010, in three different soil and climatic locations: Zemun (100 m a.s.l.), Srbobran (86 m a.s.l.) and Guča (370 m a.s.l.). The four-repplicate field trials were set up using standard methodology according to the random block desing.

The analysis of variance suggest that number of tubers per plant, number of market tubers per plant and total tuber yield were significantly fluctuating depending on genotype (G), year (Y) and the location (L). In addition to individual influences of different factors, their interactions were also pronounced (G x Y, G x L, Y x L, G x Y x L).

In the three-year period average, the highest total yield was recorded in Zemun (35.80 t ha־¹), followed by Guča (29.32 t ha־¹), while the lowest average yield recorded was in Srbobran (27.38 t ha־¹). The highest average yield of tubers in the three-year period was recorded in the Cleopatra variety, followed by Adora variety, while the lowest average yield was recorded in the variety Frisia. Obtained results show that the highest yields over observed locations were recorded in early varieties that formed medium number of tubers per plant (Cleopatra and Adora) and medium late varieties (Desiree and Kondor) that expressed good resistance to high air temperatures and stress caused by drought.

Key words: genotype, potato, tuber, varieties, yield

Corresponding author: Nebojša Momirović, Faculty of Agriculture, Nemanjina 6, 11080 Belgrade, Serbia, Tel: +381 11 2615-315; Fax: +381 11 2193-659; E-mail: emomirov@agrif.bg.ac.rs,

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Published in „GENETIKA“ Vol. 48, No. 1 (2016), pp. 125- 137

Serbia

UDC 575.630

DOI: 10.2298/GENSR1601125S

Original scientific paper

DIALLEL ANALYSIS FOR ESTIMATION OF GENETIC PARAMETERS IN ORIENTAL TOBACCO GENOTYPES

Reyhaneh SEYYED-NAZARI¹, Mortaza GHADIMZADEH¹, Reza DARVISHZADEH¹,

Seyyed Reza ALAVI²

¹Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Urmia University, Urmia, Iran

² Urmia Tobacco Research Centre (UTRC), Urmia, Iran

Abstract

Seyyed-Nazari R., M. Ghadimzadeh, R. Darvishzadeh, S. Reza Alavi (2016): Diallel analysis for estimation of genetic parameters in oriental tabacco genotypes.- Genetika, Vol 48, No.1, 125-137.

Oriental tobacco is one of the main types of tobacco (Nicotiana tabacum L.), which consists eight percent of the total world production in the world. The objective of this research was to estimate genetic parameters using diallel cross analysis to identify suitable parents used in Oriental tobacco breeding programs. Fifteen F1 progenies along with their six parents were planted in randomized complete block design with three replications in two separate experiments at Urmia Tobacco Research Centre, Iran in 2012. Combined analysis of variance for seven investigated traits consisting of fresh weight of leaf (FWL), dry weight of leaf (DWL), number of leaves (NL), length of leaf (LL), width of leaf (WL), length of stem (LS), and diameter of stem (DS), using Griffing’s method 2, Model 1 showed significant mean squares of GCA and SCA for all traits except length of leaf indicating the importance of both additive and non-additive genetic effects for these traits. Baker ratios indicated that additive genetic effect were more important for investigated traits except number of leaves in which equal importance of both components was indicated. Good and poor general combiners were determined among parents for each investigated traits and finally a set of hybrids appeared to be significant SCA which could be used in Oriental tobacco improvement.

Key words: Diallel, Gene effects, GCA, SCA, Oriental tobacco

Corresponding author: Mortaza Ghadimzadeh, Department of Plant Breeding and Biotechnology, Urmia University, Urmia, Iran., Phone: + 98 914 1491832,Fax: + 98 44 3277955,8 E-mail: m.ghadimzadeh@urmia.ac.ir,

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Published in „GENETIKA“ Vol. 48, No.1 (2016), pp. 139 -149

Serbia

UDC 575
DOI: 10.2298/GENSR1601139F

Original scientific paper

ASSESSMENT OF GENETIC DIVERSITY AND ESTIMATION OF GENETIC PARAMETERS FOR REMOBILIZATION RELATED TRAITS OF WHEAT UNDER DROUGHT CONDITIONS

Ezatollah FARSHADFAR^1*, Reza AMIRI²

¹Professor of Plant Breeding; ²Ph.D. Student of Plant Breeding, Campus of Agriculture and Natural Resources, Razi University, Kermanshah, Iran

Abstract

In order to evaluate genetic variability and estimation of remobilization related traits in wheat using biometrical genetic techniques an experiment was conducted in a randomized complete blocks design with three replicates under post-anthesis drought stress conditions in the Campus of Agriculture and Natural Resources, Razi University, Kermanshah, Iran during 2011-2012 cropping season. The results of analysis of variance showed significant differences between the genotypes for all studied traits except current photosynthesis (CP) and current photosynthesis share into kernel yield (CPSKY). High genetic gain and broad sense heritability estimates were observed for penultimate remobilization share into kernel yield (PenRSKY) and internodes remobilization share into kernel yield (IRSKY) indicating high genetic potential, low effect of environment and predominant role of additive gene effect on their expression. Spike dry matter remobilization (SDMR), spike dry matter remobilization efficiency (SDMRE) and spike remobilization share into kernel yield (SRSKY) exhibited the highest phenotypic and genetic positive correlation with kernel yield (KY). Moreover, the highest genotypic and phenotypic covariance was observed between kernel yield (KY) and SDMR, CP, SDMRE and SRSKY, respectively. The highest environmental covariance was identified between kernel yield (KY), peduncle dry matter remobilization (PedDMR) and penultimate dry matter remobilization (PenDMR), respectively. High co-heritability was detected between SDMRE and PedDMR, PedDMRE and PenDMR and between peduncle remobilization share into kernel yield (PedRSKY) and internodes dry matter remobilization efficiency (IDMRE), suggesting that selection of either of the traits would simultaneously affect the others, positively.

Key words: Co-heritability, drought, genetic gain, heritability, remobilization

Corresponding author: Ezatollah Farshadfar, Professor of Plant Breeding, Department of Agronomy and Plant Breeding, Campus of Agriculture and Natural Resources, Razi University, Kermanshah, Iran, 6715685438, Tel: +98 8330111; Mobile No: +98 9183893472; Fax No: +98 8338323731,E-mail: e_farshadfar@yahoo.com

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Published in „GENETIKA“ Vol. 48, No. 1 (2016), pp. 151-164

Serbia

UDC 575
DOI: 10.2298/GENSR1601151S
Original scientific paper

GENETIC DIVERSITY ANALYSIS OF TROPICAL SUGAR BEET (Beta vulgaris L.) VARIETIES IN BANGLADESH USING RAPD MARKERS

Saidin saclain, Md Abdul latif, Babul bala, Mithun mallik, Md. Shahidul islam^*

Department of Biotechnology, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh.

Abstract

Saclain S., MD A. Latif, B. Bala, M. Mallik, MD. S. Islam (2016): Genetic diversity analysis of tropical sugar beet (Beta vulgaris L.) varieties in Bangladesh using Rapd markers.-Genetika, Vol 48, No. 1, 151-164.

Knowledge on intra-specific genetic variation of an organism is important for its genetic improvement and conservation. In order to estimate genetic variation and relatedness in eleven tropical Sugar beet varieties we used randomly amplified polymorphic DNA (RAPD) markers. The RAPD analysis was performed using six decamer random primers, which amplified a total of 63 DNA fragments of which 43 (68.25%) were found polymorphic. The average polymorphic bands per primer was 7.17 and the overall gene diversity was 0.24. Among the 43 polymorphic loci studied, 2 were specific for 2K 310, 1 for Shubraha, 1 for Natura and 1 for HI-0473 varieties. Pair wise genetic distance and similarity indices were ranged from 0.12-0.51 and 66.73-92.91, respectively. Cauvery and 2K 310 were found to be the most distantly related with a higher genetic distance value (GD = 0.51) and lower similarity index (S_I = 66.73), while Aranka and Serenada were the most closely related with their lower GD (0.12) and higher S_I(92.91) values. In an unweighted pair group method of arithmetic mean dendrogram constructed on the basis of genetic distances, the eleven varieties grouped into two main clusters: 2K 310 alone was in one cluster whereas 10 other varieties grouped into a major cluster. This indicates that 2K 310 was distantly related with each of the other varieties. Distantly related varieties based on estimated genetic variation could be selected for future breeding program that could result in improvement of this crop.

Key words: Sugar beet; RAPD; genetic variation;polymorphic loci; gene diversity.

Corresponding author: Md. Shahidul Islam, Department of Biotechnology, Bangladesh Agricultural University, Bangladesh,E-mail: shahidj1@yahoo.com, Phone: +8801738867466; Fax: +880 91 61510

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Published in „GENETIKA“ Vol. 48, No.1(2016), pp. 165- 172

Serbia

UDC 575.633.15
DOI: 10.2298/GENSR1601165P

Original scientific paper

RELATIONSHIP BETWEEN GENETIC DISTANCE, SPECIFIC COMBINING ABILITIES

AND HETEROSIS IN MAIZE (Zea mays L.)

Jovan PAVLOV¹, Nenad DELIĆ¹, Tomislav ŽIVANOVIĆ², Danijela RISTIĆ¹, Zoran ČAMDŽIJA¹,

Milan STEVANOVIĆ¹, Miodrag Tolimir¹

¹Maize Research Institute Zemun Polje, Belgrade, Serbia

²Faculty of Agriculture – University of Belgrade, Serbia

Abstract

Pavlov J., N. Delić, T. Živanović, D. Ristić, Z. Čamdžija, M. Stevanović, M. Tolimir (2016): Relationship between genetic distance, specific combining abilities and heterosis in maize (Zea mays L.).-Genetika, Vol 48, No. 1, 165-172.

Six maize inbred lines for which we assumed that have a similar genetic background were selected for the study. Inbred lines were crossed according to incomplete diallel design and fifteen hybrid combinations were obtained. Hybrid combinations and their parental lines were used in our study. The objective of the study was to examine genetic polymorphism of parental lines, as well as to determine relationships between SSR genetic distance and values of high parent heterosis and specific combining abilities for grain yield. By using 19 SSR primers we classified inbred lines in two groups. First cluster consists of inbred lines L1, L2, L3, L4 and L5, while inbred line L6 formed second cluster, indicating different heterotic background in comparison to the other five inbred lines. The Spearman’s correlation coefficients between SSR based genetic distance and specific combining abilities for grain yield had a value of (r=0,53*), while positive correlation were also found between genetic distance and high parent heterosis (r=0,57*).

Key words: inbred lines, SSR genetic distance, Spearman’s correlation coefficients

Corresponding author: Jovan Pavlov, Maize Research Institute Zemun Polje, Slobodana Bajića 1, 11185 Zemun Polje, Serbia, Tel.: + 381 64 84 06 198,Fax: + 381 11 3756 707, e-mail: jpavlov@mrizp.rs

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Published in „GENETIKA“ Vol. 48, No. 1(2016), pp. 173- 186

Serbia

UDC 575.633

DOI: 10.2298/GENSR1601173K

Original scientific paper

BREEDING BARLEY FOR QUALITY IN TURKEY

Yuksel kaya¹*, Ramazan ayranci²

¹Bahri Dagdas International Agricultural Research Institute, Konya, Turkey

² Field Crops Department, Agricultural Faculty, Ahi Evran University, Kirsehir, Turkey

Abstract

Kaya Y. and R. Ayranci (2016): Breeding barley for quality in Turkey.- Genetika, Vol 48, No. 1, 173-186.

We evaluated a total of 411 genotypes, including 334 breeding lines with 77 checks from Barley (Hordeum vulgare L.) Breeding Program of Turkey (BBPT), based upon their grain yield (GY) and quality traits (namely protein content-PC, acid detergent fiber-ADF, thousand kernel weight-TKW, kernel size-KS and test weight-TW), during the 5 consecutive cropping seasons, from 2007-2008 to 2011-2012. Broad-sense heritability (H) values for quality traits were moderate (0.57-0.65), while it was low (0.43) for grain yield. Accordingly, grain physical features (namely TW, KS and TKW) were positively significantly correlated with GY, but negatively significantly correlated with PC. Results of our study showed that selection for GY and quality traits was less efficient than we expected, due to undesirable multi-variate correlations such as GY vs PC and low to moderate H values. Therefore, we tried to put suggestions forward to the BBPT, by following discussing about our ability to select for high GY and acceptable quality in barley.

Key words: Barley, breeding, correlation, heritability, quality.

Corresponding author: Yuksel kaya, Bahri Dagdas International Agricultural Research Institute, Konya, Turkey, e-mail: yuksel_k@yahoo.com

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Published in „GENETIKA“ Vol. 48, No. 1(2016), pp. 187-198

Serbia

UDC 575.630
DOI: 10.2298/GENSR1601187V
Original scientific paper

VEGETATIVE COMPATIBILITY AND RFLP ANALYSIS OF Colletotrichum destructivum ISOLATES FROM ALFALFA AND RED CLOVER

Tanja VASIĆ¹,Vesna KRNJAJA², Darko JEVREMOVIĆ³, Slavica STANKOVIĆ⁴, Dragan Terzić¹, Jasmina MILENKOVIĆ¹, Jordan MARKOVIĆ¹

¹Institute for Forage Crops, Kruševac, Serbia

²Institute for Animal Husbandry, Belgrade, Serbia

³Fruit Research Institute, Čačak, Serbia

⁴Maize Research Institute "Zemun Polje", Belgrade, Serbia

Abstract

Vasić, T., Krnjaja, V., Jevremović, D., Stanković, S., Terzić, D., Milenković, J., Marković, J. (2015): Vegetative Compatibility and RFLP analysis of Colletotrichum destructivum isolates from alfalfa and red clover.- Genetika, Vol 48, No. 1, 187-198.

A total of 17 isolates of Colletotrichum from alfalfa (Medicago sativa L.) and red clover (Trifolium pratense L.) plants with anthracnose symptoms were collected from 11 districts in Serbia during 2005-2010 and tested for variability in vegetative compatibility groups (VCGs) and restriction fragment length polymorphisms (RFLP). Nitrate non-utilising (nit) mutants were isolated from each of investigated C. destructivum isolates by selecting chlorate-resistant sectors on medium with chlorate. The isolates were grouped in five VCGs while one isolate was self-incompatible. No relationship was found between VCGs and geographical origin of the isolates. Restriction Fragment Length Polymorphism (RFLP) analysis of a 900 bp intron of the glutamine synthetase (GS) gene revealed a unique polymorphic profile of C. destructivum isolates, distinct from the profiles of other Colletotrichum species. An identical profile was produced for all C. destructivum isolates, regardless of their host and geographical origin. PCR-RFLP failed to detect some the Serbian C. destructivum isolates.

Key words: RFLP; VCGs; M. sativa; T. pratense; C. destructivum

Corresponding author: Tanja Vasić, Institute for Forage Crops, 37251 Kruševac, Serbia; Tel/fax:+38137441295; E-mail: tanja.vasic@ikbks.com.

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Published in „GENETIKA“ Vol. 48, No. 1(2016), pp.199-210

S. Bajića 1, 11185 Belgrade-Zemun

Serbia

UDC 575
DOI: 10.2298/GENSR1601199A

Original scientific paper

NOVEL SINGLE NUCLEOTIDE POLYMORPHISMS IN LACTOFERRIN GENE AND THEIR ASSOCIATION WITH MASTITIS SUSCEPTIBILITY IN HOLSTEIN CATTLE

A.I ateya^a*, Y.Y el-seady^b, S.M. atwa ^c, B.H. merghani ^b and N.A. sayed ^b

^aDepartment of Animal Husbandry and Animal Wealth Development, Faculty of Veterinary Medicine, Mansoura University, Egypt.

^bDepartment of Physiology, Faculty of Veterinary Medicine, Mansoura University, Egypt.

^cDepartment of Internal Medicine, Infectious and fish Diseases , Faculty of Veterinary Medicine, Mansoura University, Egypt.

Abstract

ateya A.I, Y.Y el-seady, S.M. atwa, B.H. merghani and N.A. sayed (2016): Novel single nucleotide polymorphisms in lactoferrin gene and their association with mastitis susceptibility in Holstein cattle - Genetika, Vol 48, No. 1,199 -210.

Since lactoferrin is acute phase protein and has an exceptional role in defense mechanism of mammary gland, it is considered a candidate gene for mastitis susceptibility in dairy cattle. In this study, blood samples were collected for DNA extraction from fifty Holstein dairy cows in the third lactation season reared under Egyptian conditions assigned into three groups mastitis (n=15), subclinical mastitis (n=20) and healthy (n=15) based on California mastitis test. Moreover biochemical markers for inflammation were determined to detect severity. PCR amplification of a segment of 6^th intron of lactoferrin gene yielded a fragment of 301-bp in all animals under study. For revealing polymorphism, DNA sequencing was done for PCR products of lactoferrin gene (301-bp) in only five healthy (resistant) and five affected animals. Results indicated that, association between biochemical data and affections. The PCR-DNA sequencing genetic assessment identified twelve SNPs in the bovine lactoferrin gene and there was association between these identified SNPs and mastitis susceptibility, where ten nucleotide sequence variations for one of the healthy animals were obtained with A166T, T185A, T200C, G230C, A239G, T251G, A254C, A272C, T273C and A296G SNPs (submitted to GenBank with accession number gb|KT159457|. On the other hand, two nucleotide sequence variations for two of the affected animals were obtained with A100G and T275A SNPs. Consequently, the identified SNPs in the bovine lactoferrin gene can be used as marker-assisted selection (MAS) to predict, improve mastitis resistance and minimize incidence of mastitis infection in Holstein dairy cows.

Key words: Holstein cattle, lactoferrin gene, mastitis susceptibility, DNA Sequencing.

Corresponding author: A.I ateya, Department of Animal Husbandry and Animal Wealth Development, Faculty of Veterinary Medicine, Mansoura University, Egypt.Tel: +2-01003541921, FAX: +2-050-2372592,E-mail address: ahmed_ismail888@yahoo.com

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Published in „GENETIKA“ Vol. 48, No. 1(2016), pp. 211-218

S. Bajića 1, 11185 Belgrade-Zemun

Serbia

UDC 575
DOI: 10.2298/GENSR1601211Z

Original scientific paper

Prosystemin identification in Amaranthus cruentus and

A. hypochondriacus x hybridus based on data mining and sequence alignment

Jana ŽIAROVSKÁ¹*, Michal ZÁHORSKÝ², Andrea HRICOVÁ²

¹ Department of Genetics and Plant Breeding, Slovak University of Agriculture in Nitra, Slovak Republic

² Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Nitra, Slovak Republic

Abstract

Žiarovská J., M. Záhorský, A. Hricová (2016): Prosystemin identification in Amaranthus cruentus and A. hypochondriacus x hybridus based on data mining and sequence alignment.- Genetika, Vol 48 No. 1, 211-218.

Bioinformatic tool have became an inevitable part of molecular genetic research in many applications. In the present study, an in silico based approach was used to find conservative region of currently known prosystemin gene sequences and its PCR identification was performed in Amaranthus cruentus and Amaranthus hypochondriacus x hybridus. Identification results were veryfied by direct sequencing of obtained amplicons. For both of analysed species, the prosystemin sequence specifity was confirmed by sequencing. For a hybrid A. hypochondriacus x hybridus no differences were returned in bioinformatic alighment for known prosystemin sequence. For A. cruentus, two bases were found as to be different in the DNA sequence of prosystemin.

Key words: A. caudatus, A.hypochondriacus x hybridus, bioinformatic identification, prosystemin

Corresponding author: Jana Žiarovská, Department of Genetics and Plant Breeding, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 94976 Nitra, Slovak Republic Tel: e-mail: jana.ziarovska@uniag.sk

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Published in „GENETIKA“ Vol. 48, No. 1(2016), pp. 219-232

Serbia

UDC 575.633.11
DOI: 10.2298/GENSR1601219D

Original scientific paper

COMPARISON OF SIMILARITY COEFFICIENTS USED FOR CLUSTER ANALYSIS BASED ON SSR MARKERS IN SISTER LINE WHEAT CULTIVARS

Srbislav DENČIĆ^1,*, Ron DEPAUW², Vojislava MOMČILOVIĆ¹

and Vladimir AĆIN¹

¹Institute of Field and Vegetable Crops, M. Gorki St. 30, 21000 Novi Sad, Serbia,

²Semiarid Prairie Agricultural Research Centre, AAFC, P.O. Box 1030, Swift Current, SK, Canada, S9H 3X2.

Abstract

Denčić S, R. Depauw, V. Momčilovic and V. Aćin (2016): Comparison of similarity coefficients used for cluster analysis based on SSR markers in sister line wheat cultivars.- Genetika vol48, no1, 219-232.

The objective of this study was to compared fourteen different similarity coefficients and their influence in sister line wheat cultivars clustering. Seventeen sister cultivars developed from two crosses were used and fingerprinted with 19 wheat microsatellite markers. Comparisons among the similarity coefficients were made using the Sperman correlation analysis, dendogram evaluation (visual inspection and consensus fork index - CI_c), projection efficiency in a two-dimensional space, and groups formed by the Tocher optimization procedure. The Sperman correlation coefficients among the fourteen similarity coefficients were all high showing a strong association between them. The correlation coefficient between Dice and Kulczinski and Ochiai I as well as between Hamann and Simple matching and between Kulczinski and Ochiai I was equal to 1. Although visual estimation of the dendograms shows almost identical clustering structures, CI_c indexes indicate that all coefficients are not identical.

Key words: Similarity coefficient, Cluster analysis, SSR markers, Sperman correlation, Consensus fork index, Distortion degree, Stress value

Corresponding author: Srbislav Denčić, Institute of Field an Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad, Serbia; Phone: 381214898233; Fax: 381214898227 E-mail: srbislav.dencic@nsseme.com

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Published in „GENETIKA“ Vol. 48, No. 1(2016), pp. 233-248

Serbia

UDC 575
DOI: 10.2298/GENSR1601233E

Original scientific paper

DIVERSITY IN GROWTH AND EXPRESSION PATTERN OF POHKT1 AND POVHA TRANSPORTER GENES UNDER NaCl STRESS IN Portulaca oleracea TAXA

R. EL-BAKATOUSH^Ia*, A. ELFRAMAWY^b

^a*Biology and Geology Sciences Department, Faculty of Education, University of Alexandria, Egypt

^bNucleic Acids Research Department, Genetic Engineering & Biotechnology Research Institute (GEBRI), Mubarak City for Scientific Research Technology Applications, Egypt

Abstract

El-Bakatoushi R., A. Elframawy (2016): Diversity in growth and expression pattern of pohkt1 and povha transporter genes under NaCl stress in Portulaca oleracea taxa.- Genetika vol 48, no1, 233-248.

Plant growth and the expression of two transporter genes; PoHKT1 and PoVHA transcripts in root and shoot tissues were studied under salt stress of three Portulaca oleracea s.l. taxa. The study showed no significant differences in ratios between root lengths in saline and non-saline treatments of the three taxa, which was correlated with a clear down-regulation of the PoHKT1 transcripts in the root after 150mM NaCl. All measured growth parameters except root length increased in P. oleraceae, decreased in P. granulatostellulata and remain unchanged after 100mM NaCl in P. nitida compared to control under saline conditions. The result was consistent with the type of taxon which had significant effect on the shoot length, number of leaves and dry weight (P< 0.05). All measured growth parameters except root length showed a significant negative correlation with the shoot fold change of PoHKT1 transcripts (r = -0.607, -0.693 and -0.657 respectively). The regulation of PoVHA in root and shoot tissues in the three taxa are significantly different. Under salt stress, both decreased uptake of Na+ into the cytosol by decreasing the expression of PoHKT1 and increased vascular compartmentalization ability of Na+ by inducing the expression of PoVHA seem to work more efficiently in P. oleraceae and P. nitida than in P. granulato-stellulata

Key words: Salt stress; growth; Purslane; RT-PCR; PoHKT1; PoVHA

Corresponding author: Ranya El-Bakatoushi, Biology and Geology Sciences Department,Faculty of Education,University of Alexandria, Egypt, Tel. 0102417351, .Fax. +034865671,Email: ranyaelbakatoushi@ edu.alexu.edu.eg

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Published in „GENETIKA“ Vol. 48, No. 1(2016), pp. 249-269

Serbia

UDC 575.633.11

DOI: 10.2298/GENSR1601249D

Original scientific paper

QUALITY PROPERTIES AND EXPRESSION PROFILING OF PROTEIN DISULFIDE ISOMERASE GENES DURING GRAIN DEVELOPMENT OF THREE SPRING WHEAT NEAR ISOGENIC LINES

Liwei DONG^1,4, Ning LI^1,4, Xiaobing LU^1,4, Slaven PRODANOVIC^2*, Yanhao XU³,

Wenying ZHANG³, Yueming YAN^1,3*

¹College of Life Science, Capital Normal University, 100048 Beijing, China

²Hubei Collaborative Innovation Center for Grain Industry, 434025 Jingzhou, China

³Faculty of Agriculture, University of Belgrade, Belgrade, Serbia

⁴Contributed equally to this work

Dong L., N. Li, X. Lu, S.Prodanovic, Y.Xu, W. Zhang, Y. Yan (2016): Quality properties and expression profiling of protein disulfide isomerase genes during grain development of three spring wheat near isogenic lines.- Genetika vol48, no1, 249-269.

Three wheat glutenin near isogenic lines (NILs) CB037A, CB037B and CB037C were used to investigate their quality properties and the transcriptional expression profiles of PDI gene family during grain development. Our purpose is to understand the relationships between the dynamic expression of different PDI genes and glutenin allelic compositions related to gluten quality. The results showed that glutenin allelic variations had no significant effects on main agronomic traits and yield performance, but resulted in clear gluten quality changes. CB037B with 5+10 subunits had higher glutenin macropolymer (GMP) content and better breadmaking quality than CB037A with 2+12 while the lack of Glu-B3h encoding one abundant B-subunit in CB037C significantly reduced GMP content, dough strength and breadmaking quality. The dynamic expression patterns of eight protein disulfide isomerase (PDI) genes during grain development detected by quantitative real-time polymerase chain reaction (qRT-PCR) showed the close correlations between higher expression levels of PDI3-1, PDI5-1 and PDI8-1 and the presence of 5+10 subunits. Meanwhile, Glu-B3h silence resulted in significant decrease of expression levels of five PDI genes (PDI3-1, PDI5-1, PDI6-1, PDI7-2 and PDI8-1), suggesting the vital roles of certain PDI genes in glutenin and GMP synthesis and gluten quality formation.

Key words: Wheat, NILs, glutenins, PDIs, qRT-PCR

Corresponding author: Prof. Dr. Slaven Prodanovic, Faculty of Agriculture, University of Belgrade, Nemanjina 6, 11080, Belgrade, Serbia, phone: +381638687246, fax: +38-11-13161987, E-mail: slavenp@agrif.bg.ac.rs

Prof. Dr. Yueming Yan, College of Life Science, Capital Normal University, Beijing, XisanhuanBeilu 105, 100048 Beijing, China, phone: +86-10-68902777, fax: +861068902777, E-mail: yanym@cnu.edu.cn

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Published in „GENETIKA“ Vol. 48, No. 1(2016), pp. 271-284

Serbia

UDC 575

DOI: 10.2298/GENSR1601271C

Original scientific paper

STABILITY AND PATH ANALYSIS FOR YIELD AND RELATED TRAITS

IN SILKWORM, (Bombyx mori L.) REARED UNDER STRESS CONDITIONS

n. chandrakanth, s.m. moorthy^*, m. rekha and v. sivaprasad

Silkworm Crop Improvement, Central Sericultural Research and Training Institute, Mysore, Karnataka, India

Abstract

chandrakanth n., s.m. moorthy^*, m. rekha and v. sivaprasad (2016): Stability and path analysis for yield and related traits in silkworm, (Bombyx mori L.) reared under stress conditions- Genetika, Vol 48, No1, 271-284.

The silkworm, Bombyx mori L. is a poikilothermic organism whose growth and development is adversely affected by high temperatures. Rearing of silkworm at high temperature conditions of tropical countries like India causes extensive crop loss by reducing cocoon yield. Breeding new silkworm breeds tolerant to high temperatures is the most effective and economical method to increase cocoon yield. Introduction of stable genotypes in such breeding programs and knowledge on the relationships between cocoon yield and other traits would be more advantageous in increasing cocoon yield at high temperatures. Therefore, in this study twenty silkworm genotypes were reared in five artificially simulated environments with different temperatures ranging from 25°C to 38°C. Based on coefficient of variation (%) values, the traits cocoon yield / 10,000 larvae by weight (CY) and cocoon weight showed maximum and minimal variation among the traits in all the five environments. Analysis of variance revealed a substantial genetic variability among the genotypes for most of the traits studied. The results of combined analysis of variance showed significant differences over traits indicating that the traits are influenced by genotype × environment interactions. Stability analysis with respect to CY showed higher mean value compared to grand mean, regression coefficient equal to unity and less deviation from regression in ATR16, CSR17, BHR2, S-5 and D13 genotypes, suggesting that they are consistent with the CY over the different environments. Clustering based on the stability parameters resulted in separation of twenty silkworm genotypes into 5 clusters based on their ability to tolerate high temperatures. Correlation studies demonstrated positive and significant (P < 0.05) correlations between CY and other traits. Pupation rate was most positively associated with CY followed by larval weight, cocoon weight, cocoon shell weight and cocoon shell percent. Path coefficient analysis also revealed that the pupation rate had the maximum direct effect on CY followed by cocoon shell weight and larval weight. Cocoon shell percent and cocoon weight exhibited negative direct path effect on cocoon yield / 10,000 larvae by weight. Therefore, the selection based on pupation rate, larval weight and cocoon shell weight can be effective in improving cocoon yield in silkworm at different environments with varying temperature.

Key words: Silkworm, Cocoon yield, Stability analysis, Correlation, Path coefficient analysis

Corresponding author: Dr. S.M. Moorthy,Silkworm Breeding and Molecular biology Laboratory,Silkworm Crop Improvement Division,Central Sericultural Research and Training Institute, Srirampura, Manandavadi Road, Mysore 570008, Karnataka, India,Phone: +91-9481821244,Fax: +91-821-2362845,e-mail: moorthysm68@gmail.com

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Published in „GENETIKA“ Vol. 48, No. 1(2016), pp. 285-295

Serbia

UDC 575

DOI: 10.2298/GENSR1601285I

Original scientific paper

CHARACTERIZATION OF Pseudomonas syringae PATHOVARS FROM DIFFERENT SWEET CHERRY CULTIVARS BY RAPD ANALYSIS

Renata ILIČIĆ⁽¹⁾, Jelica BALAŽ⁽¹⁾, Vera STOJŠIN⁽¹⁾, Dragana JOŠIĆ⁽²⁾

⁽¹⁾University of Novi Sad, Faculty of Agriculture, , Novi Sad, Serbia.

⁽²⁾ Institute of Soil Science, Genetic Lab, Belgrade, Serbia.

Abstract

Iličić R., J. Balaž, V.Stojšin, D. Jošić (2016): Characterization of Pseudomonas syringae pathovars from different sweet cherry cultivars by RAPD analysis.- Genetika vol 48, no1, 285-295.

Pseudomonas syringae pvs., isolated from sweet cherry grown on different localities in Serbia, were genetically characterized using RAPD analysis. Four out of eleven tested primers (SPH1, DJP 17, DJ 15, and DJ 16) were selected on the basis of the differences between isolates within two pathovars - syringae and morsprunorum race 1. Cumulative RAPD analysis indicated heterogeneity within the population of both groups of tested isolates, revealing four different patterns in each group. RAPD analysis showed up to 24% differences among pv. syringae isolates, as well as 41% in comparison with the reference strain KFB0103 (pv. syringae), while differences of 15% among isolates pv. morsprunorum 1 race and 36% compared to the reference strain CFBP2119 (pv. morsprunorum 1) were observed. Isolates from locality Selenca exhibited three different genotypic patterns of pv. morsprunorum race 1 and one pattern of pv. syringae. Isolates of pv. morsprunorum collected in the same year from two plant organs (branches and leaves) of the cv. Vanda yielded two different patterns. The pv. morsprunorum on cv. Kordia and pv. syringae on cv. Regina were detected at Mikicevo locality. The same patterns were observed for isolates of pv. syringae from Kanjiza and Selenca, as well as from Gornji Tavankut in two years of isolation. Differences were noted between isolates from the same pathovar originating from Ljutovo and Mikicevo, as well as with respect to all other isolates of same pathovar.

Key words: Pseudomonas syringae pv. syringae, P. syringae pv. morsprunorum race 1, sweet cherry, RAPD.

Corresponding author: Renata Iličić, University of Novi Sad, Faculty of Agriculture, Trg Dositeja Obradovića 8, 21000 Novi Sad, Serbia

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Published in „GENETIKA“ Vol. 48, No. 1(2016), pp 297-305

Serbia

UDC 575.633.15

DOI: 10.2298/GENSR1601297A

Original scientific paper

GENOTYPIC EFFECTS ON BORON CONCENTRATIONS AND RESPONSE ON BORON FERTILIZATION IN MAIZE INBRED LINES

Luka ANDRIĆ¹, Vlado KOVAČEVIĆ², Imre KADAR³, Antun JAMBROVIĆ¹,

Hrvoje PLAVŠIĆ¹ and Domagoj ŠIMIĆ¹

¹Agricultural Institute Osijek, Osijek, Croatia

²Faculty of Agriculture of University J. J. Strossmayer in Osijek,

Osijek, Croatia

³ Research Institute for Soil Science and Agricultural Chemistry (RISSAC),

Budapest, Hungary

Abstract

Andrić L., V. Kovačević, I.Kadar A. Jambrović, H. Plavšić and D. Šimić (2015): Genotypic effects on boron concentrations and response on boron fertilization in maize inbred lines- Genetika, Vol 48, No. 1,297 - 305.

Boron (B) deficiency in maize can result in barren cobs attributed to silks being non-receptive which is particularly important for the female parent in seed production. The objectives of this study were 1) to investigate genotypic differences among nine female inbred lines used in seed production for B concentration in ear-leaf and grain, as well as for grain yield and moisture in a three-year experiment (2006-2008) and 2) to determine response and relations among the traits when four of the female inbred lines are treated by foliar boron fertilization - three times in 10-days interval with 0.5% Solubor solution (17.5% B) during one growing season (2008). The investigations were performed on Experimental field of Agricultural Institute Osijek, (soil type: eutrical cambisol). Highly significant differences among the nine female inbred lines were detected for B concentration in ear-leaf (from 14.7 to 46.7 mg B kg^-1) and grain (from 1.20 to 2.06 mg B kg^-1) as well as for grain yield (from 3.33 to 4.83 t ha^-1) and grain moisture (from 14.7% to 26.6%). However, there were also significant effects of growing season and the genotype by environment interaction for all four traits. Positive and moderate correlations were found between the boron status in plant and grain yield. Although B concentrations were considerably increased by foliar boron fertilization (averages 41.7 and 125.3 mg B kg^-1 in leaves, 1.79 and 2.80 mg B kg^-1 in grain, for control and fertilization, respectively), in general grain yield differences among treatments were non-significant. (averages 5.21 and 5.15 t ha^-1, respectively).

Key words: boron, female parent inbred lines, foliar fertilization, grain, leaves, maize, yield

Corresponding author: Vlado Kovačević, Faculty of Agriculture, Kralja P. Svačića 1d,31000 Osijek, Croatia; Phone: ++385 31 554 932, e-mail: vkovacevic@pfos.hr

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Published in „GENETIKA“ Vol. 48, No. 1(2016), pp. 307-322

UDC 575.630

DOI: 10.2298/GENSR1601307K

Original scientific paper

MOLECULAR ANALYSIS OF COMMERCIAL DATE PALM CULTIVARS IN LYBIA USING ISSR AND SRAP PCR- BASED MARKERS

Noha S. khalifa¹, Maher M. shehata¹, Ahmed F. abodoma², Abdullah F. al-kumbezy³

¹Unit of Plant Genetics, Department of Botany, Faculty of Science, Ain Shams University, Cairo, Egypt.

² Department of Genetics, Faculty of Agriculture, Ain Shams University, Cairo, Egypt ³ Department of Biotechnology, Faculty of Science, Margob University, Libya.

Abstract

khalifa S. N., M. M. shehata, A. F. abodoma, A. F. al-kumbezy (2016): Molecular analysis of commercial date palm cultivars in Lybia using ISSR and SRAP PCR- based markers - Genetika, Vol 48, No. 1, 307 -322.

Little is known about the molecular structure of the date palm (Phoenix dactylifera L.) despite its importance as invaluable drought tolerant crop. Intervarietal variation and cultivar identification are crucial for breeding and gene bank conservation of this plant worldwide. In this work, two PCR based marker systems (ISSR and SRAP) were applied on top quality eight commercial cultivars in Libya (Umfetity, Bekrary, Alhamraya, Sufeer Genab, Alsaeedy Show, Farag Barameel, Majhool Alheelo and Alkhadraya). DNA variations were explored using eleven ISSR and nine combinations of SRAP markers. All markers used generated polymorphic bands among the different cultivars that can be used as molecular markers for their differentiation. The genetic distance between cultivars was also estimated from banding patterns. Our results indicate that ISSR and SRAP systems can efficiently identify and differentiate between the selected cultivars. This work can be used as a model to establish a road map for all date palm cultivars worldwide.

Key words: DNA markers, Gene diversity, Nie genetic distance coefficient, Phoenix dactylifera , Phylogenetic tree.

Corresponding author: Noha S. khalifa,Unit of Plant Genetics, Department of Botany, Faculty of Science, Ain Shams University, Cairo, Egypt, Mobile phone: +02 21009123211, NohaKhalifa@hotmail.com

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Published in „GENETIKA“ Vol. 48, No. 1(2016), pp. 323-338

Serbia

UDC 575.630

DOI: 10.2298/GENSR1601323J

Original scientific paper

MORPHO- BIOCHEMICAL EVALUATION OF Brassica rapa SUB-SPECIES FOR SALT TOLERANCE

Sohail Ahmad JAN¹*, Zabta Khan SHINWARI¹, Malik Ashiq RABBANI²

¹Department of Biotechnology, Quaid-i- Azam University, Islamabad, Pakistan

²Plant Genetic Resources Institute, National Agricultural Research Centre, Islamabad, Pakistan

Abstract

Jan A. S., Z. K.Shinwari, M. A.Rabbani (2016): Morpho- biochemical evaluation of Brassica rapa sub-species for salt tolerance- Genetika, Vol 48, No.1, 323 -338.

Salt stress is one of the key abiotic stresses that affect both the qualitative and quantitative characters of many Brassica rapa sub-species by disturbing its normal morpho-biochemical processes. Therefore, the present research work was designed to study the effect of different NaCl events (0, 50,100 and 150 mmol) on morphological and biochemical characters and to screen salt tolerant genotypes among brown, yellow and toria types of B. rapa sub-species. The plants were grown in test tubes with addition of four level of NaCl (0, 50,100 and 150 mmol). The effect of salinity on shoot and root length, shoot/ root fresh and dry weight, relative water content (RWC), proline and chlorophyll a, b, a+b contents was recorded after 4 weeks of sowing. The genotype 22861 (brown type) showed excellent morphological and biochemical performance at all stress levels followed by Toria-Sathi and Toria-A respectively as compared to Check variety TS-1. The genotype 26158 (yellow type) gave very poor performance and retard growth. The %RWC values and chlorophyll a, b and a+b contents were decreased several folds with the increase of salt concentration. While, the proline contents was increased with raising of salt stress. The brown and toria types showed maximum tolerance to salt stress at early germination stages as compare to yellows one. The present study will serve as model to develop quick salt tolerant genotypes among different plant sub-species against salt stress.

Key words: Brassica rapa, early germination, salt stress, salt tolerance.

Corresponding author: Sohail Ahmad Jan, Department of Biotechnology, Quaid-i-Azam University, Islamabad, Pakistan ,Phone: +92-345-9118133, E-mail: sjan.parc@gmail.com; sohailahmadjan3@gmail.com

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Published in „GENETIKA“ Vol. 48, No. 1(2016), pp. 339-348

Serbia

UDC 575.630
DOI: 10.2298/GENSR1601339D

Original scientific paper

EVALUATION OF SUGAR BEET GENOTYPES FOR ROOT TRAITS

BY PRINCIPAL COMPONENT ANALYSIS AND CLUSTER ANALYSIS

Dario DANOJEVIĆ¹, Živko ĆURČIĆ¹, Nevena NAGL¹, Ksenija TAŠKI-AJDUKOVIĆ¹,

Jan BOĆANSKI²

¹Institute of Field and Vegetable Crops, Novi Sad, Serbia

²Faculty of Agriculture, University of Novi Sad, Serbia

Abstract

DanojevićD., Ž. Ćurčić, N. Nagl, K. Taški-Ajduković, J. Boćanski (2016): Evaluation of sugar beet genotypes for root traitsby principal component analysis and cluster analysis - Genetika, Vol 48, No.1, 339 -348.

Sugar beet is the most important crop for sugar production in Europe. Wide genetic variability is essential in sugar beet breeding programs. The aim of this study is to evaluate variability for the main root traits and differences between monogerm and multigerm sugar beet genotypes from the breeding collection at the Institute of Field and Vegetable Crops. The following traits were analyzed: root weight (g), dry matter content (%), root head weight (g), root/head ratio (%), number of cambial rings, root length (cm) and root diameter (cm). Mean values for two years per genotype were standardized and used for analysis. Principal Component Analysis (PCA) and Cluster Analysis (CA) were used to examine the level of diversity for 20 genotypes and to rank the contributions of the variables. According to CA genotypes could be placed into five main groups, where a large number of multigerm genotypes were put in one group. On average multigerm genotypes were characterized by higher mean values for root weight, length, diameter and lower root head ratio. Multigerm genotypes had higher coefficients of variation for nearly all measured root traits.

Key words: multivariate analysis, root,sugar beet

Corresponding author: Dario Danojević, Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad,Phone 021 4898 352, Fax 021 4898 355,E-mail: dario.danojevic@ifvcns.ns.ac.rs

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Published in „GENETIKA“ Vol. 48 No. 1(2016), pp. 349-362

Serbia

UDC 575
DOI: 10.2298/GENSR1601349B
Original scientific paper

CHARACTERIZATION OF THE VINEYARD BYOTYP COLLECTION OF PEACH AS STEP IN PREBREEDING PROCESS

Ivana bakić^1*, Vera rakonjac², Dragan nikolić², Milica fotirić-akšić²,

Slavica čolić¹, Aleksandar radović²

¹ Institute for Science Application in Agriculture, Belgrade, Serbia

² University of Belgrade, Faculty of Agriculture, Belgrade, Serbia

Abstract

bakić I., V. rakonjac, D. nikolić, M. fotirić-akšić, S. čolić, A. radović (2016): Characterization of the vineyard byotyp collection of peach as step in prebreeding process- Genetika, Vol 48, No.1, 349 -362.

In this research, morphological characterization of vineyard peach collection was conducted. In 78 accessions thirty qualitative traits of tree, flower, leaf, fruit and stone were analysed by using UPOV and ECPGR descriptors. Most of the studied traits showed a high degree of variability. The lowest variability obtained for the leaf traits, and highest for skin and flesh colour. Not only were the accessions sorted into a large number of categories by the most of their properties, but also there was a significant level of variability in the collection, reflecting in the fact that the traits were recombinant in a different way. Principal component analysis (PCA) and a dendrogram were performed to determine relationships among accessions and to obtain information on the usefulness of those characters for the discrimination. The PCA revealed that the first 4 principal components were able to represent 43.1% of total variance. Traits with high discriminating values comprised internal and external fruit colour, flower type and colour, flower bud density and stone shape. The cluster analysis showed that the accessions were placed in three main clusters. The greatest impact on the separation in clusters had fruit over colour and extent of fruit over colour. Moreover, the collection can also comprise some accessions with the preferred recombination of properties that might be interesting for further studies in breeding.

Key words: Morphological characterisation, multivariate analysis, vineyard peach

Corresponding author: Ivana Bakić, bul. Despota Stefana 68b; telefon: 011/ 2751-622; fax: 011/2752-959; email: ibakic@ipn.co.rs

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Published in „GENETIKA“ Vol. 48, No.1(2016), pp. 363-372

Serbia

UDC 575

DOI: 10.2298/GENSR1601363A

Original scientific paper

THE ASSOCIATION BETWEEN ADIPONECTIN AND ADIPOQ GENE POLYMORPHISMS WITH OBESITY AMONG YOUNG JORDANIAN WOMEN

¹Mahmoud A. ALOMARI, Omar F. KHABOUR, ²,Asmaa A. ABU OBAID²

¹Division of Physical Therapy, Department of Rehabilitation Sciences, Jordan University of Science and Technology, Irbid, Jordan

²Department of Medical Laboratory Sciences, Jordan University of Science and Technology, Irbid, Jordan

Abstract

AlomariA.M., O. F. Khabour, A. A. Abu Obaid (2016): The association between adiponectin and adipoq gene polymorphisms with obesity among young Jordanian women- Genetika, Vol 48, No.1,363 -372.

Obesity is a risk for multiple diseases and an independent cause of morbidity and mortality with staggering global rates. Adiponectin is an adipocyte-derived peptide associated with reduced obesity. In this study, the effect of ADIPOQ gene single nucleotide polymorphisms (SNPs) on obesity and adiponectin relationship was examined. The study was conducted on 389 adult females. Obesity was measured using body weight, BMI, percent body fat, and waist and hip ratio. ADIPOQ G276T and I164T SNPs were genotyped using RFLP procedure. Adiponectin plasma levels were quantified using ELISA technique. Adiponectin correlated with all obesity measures (p<0.05). However, when divided according to genotypes, adiponectin remained correlating (p<0.05) with obesity measures in the participants with GT of the G276T SNP but not (p>0.05) with GG and TT of the G276T SNP. With respect to` I164T SNP, the correlations between adiponectin and obesity measures remained in all genotypes except with W/H ratio and %Bf remained in the participants with CC genotype and with W/H ratio in CT/TT genotypes. Further analyses reveled that adiponectin was lower (p<0.05) in the participants with GT versus the GG and TT genotypes of G276T SNP. The data confirms the effect of adiponectin for obesity. It also shows the importance of ADIPOQ SNPs in the relationship between adiponectin and obesity in young adult females.

Key words: Women obesity; ADIPOQ; G276T and I164T SNPs; BMI; Waist/Hip

Corresponding author: Mahmoud Alomari, PhD,Faculty of Applied Medical Sciences,Jordan University of Science and Technology,Irbid, Jordan,Email: Alomari@just.edu.jo

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Published in „GENETIKA“ Vol. 48, No.1(2016), pp.373-381

Serbia

UDC 575.630

DOI: 10.2298/GENSR1601373P

Original scientific paper

COMPARATIVE STUDY OF DROUGHT AND SALT STRESS EFFECTS

ON GERMINATION AND SEEDLING GROWTH OF PEA

Gordana PETROVIĆ, Dušica JOVIČIĆ, Zorica NIKOLIĆ, Gordana TAMINDŽIĆ,

Maja IGNJATOV, Dragana MILOŠEVIĆ, Branko MILOŠEVIĆ

Institute of Field and Vegetables Crops, Novi Sad, Serbia

Abstract

Petrović G., D. Jovičić, Z. nikolić, G. Tamindžić, M. Ignjatov, D. Milošević, B. Milošević (2016): Comparative study of drought and salt stress effects on germination and seedling growth of pea- Genetika, Vol 48, No.1,373 -381.

Seed germination is first critical and the most sensitive stage in the life cycle of plants compromise the seedlings establishment. Salt and drought tolerance testing in initial stages of plant development is of vital importance, because the seed with more rapid germination under salt or water deficit conditions may be expected to achieve a rapid seedling establishment, resulting in higher yields. The aim of this study was to determine whether the pea seed germination and seedling growth were inhibited by the salt toxicity and osmotic effect during the seedling development, and also identification of the sensitive seedling growth parameters in response to those stresses. Based on the obtained results, pea has been presented to be more tolerant to salt than water stress during germination and early embryo growth. Investigated cultivars showed greater susceptibility to both abiotic stresses when it comes growth parameters compared to seed germination.

Key words: abiotic stress, Pisum sativum, NaCl, PEG

Corresponding author: Gordana Petrović,Institute of Field and Vegetable Crops,Adress: Maksima Gorkog 30, 21000 Novi Sad, Serbia Phone: 021/4898-154, Fax: 021/421-249,

e-mail:gordana.petrovic@nsseme.com

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Published in „GENETIKA“ Vol. 48, No.1(2016), pp.383-394

Serbia

UDC 575.630
DOI: 10.2298/GENSR1601383A

Original scientific paper

Variability and heritability of floral development in apple full-sib offsprings

Ayşe Nilgün ATAY, Şerif ÖZONGUN, Turgay SEYMEN, Alamettin BAYAV,

^*Ersin ATAY

TAGEM, Fruit Research Institute,32500 Eğirdir, Isparta, Turkey

Atay N. A. Ş. Özongun, T. Seymen, A. Bayav, E. Atay (2016): Variability and heritability of floral development in apple full-sib offsprings- Genetika, Vol 48, No.1, 383 -394.

The timing of blooming in spring is highly crucial for temperate zone tree fruit production. In this study, we aimed to investigate floral development using the all parents and full-sib offsprings of two different crossing combinations (‘Kaşel 37’ × ‘Delbarestivale’ and ‘Kaşel 41’ × ‘Williams’ Pride’). Assessments of variability in floral development have been done according to a numerical assessment scheme defined by ten stages (stage 0–9). The assessments were conducted three different dates, early (21 04), intermediate (28 04), and late (05 05). The results showed significant variations for floral development among the 273 genotypes. In particular, the genotypes 326, 340, 369, 88 and 146 were found as superiors for floral development. The broad sense heritability (h²_b) for floral development was reliable on the first assessment date, with no reliability on the other assessment dates. Our results would be useful to geneticists and breeders.

Key words: Amasya, breeding, F1, Malus slyvestris, new cultivar, progeny, spring frost.

Corresponding author: Ersin Atay, TAGEM, Fruit Research Institute,32500 Eğirdir, Isparta, Turkey, atayersin@yahoo.com

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Published in „GENETIKA“ Vol. 48, No.1(2016), pp. 395-408

Serbia

UDC 575

DOI: 10.2298/GENSR1601395V

Original scientific paper

ANALYSIS OF ASSOCIATION BETWEEN POLYMORPHISMS OF MTHFR, MTHFD1 AND RFC1 GENES AND EFFICACY AND TOXICITY OF METHOTREXATE IN RHEUMATOID ARTHRITIS PATIENTS

Dubravka VEJNOVIC¹, Vera Milic², Tatjana DAMNJANOVIC¹, Nela MAKSIMOVIC¹, Vera BUNJEVACKI¹, Ljiljana LUKOVIC¹, Ivana NOVAKOVIC¹, Maja KRAJINOVIC³, Nemanja DAMJANOV², Goran RADUNOVIC², Sofija PAVKOVIC-LUCIC⁴ and Biljana JEKIC¹

¹Institute of Human Genetics, Faculty of Medicine, University of Belgrade, Belgrade, Serbia

²Institute of Rheumatology, Faculty of Medicine, University of Belgrade, Belgrade, Serbia

³Canada Service d'Hématologie-Oncologie, Centre de Recherche, Hôpital Sainte-Justine, Montréal, Quebec, H3T 1C5 Canada

⁴Faculty of Biology, University of Belgrade, Belgrade, Serbia

Abstract

Vejnovic D., V. Milic, T. Damnjanovic, N.Maksimovic, V. Bunjevacki, Lj. Lukovic, I.Novakovic, M. Krajinovic, N. Damjanov, G.Radunovic, S. Pavkovic-Lucic and B.Jekic (2016): Analysis of association between polymorphisms of mthfr, mthfd1 and rfc1 genes and efficacy and toxicity of methotrexate in rheumatoid arthritis patients- Genetika, Vol 48, No.1,395 -408.

A folate analogue methotrexate (MTX) is the most commonly used disease-modifying drug in the treatment of rheumatoid arthritis. However, the clinical response of RA patients treated with MTX shows interindividual differences and 30% of patients discontinue therapy due to the side effects. In a group of 184 RA patients treated with MTX we have investigated whether polymorphisms in MTHFR (rs1801133, rs1801131), MTHFD1 (rs2236225) and RFC1 (rs144320551) genes may have impact on MTX efficacy and/or adverse drugs effects (ADEs).

The efficacy of the MTX therapy has been estimated using the disease activity score in 28 joints (DAS28-ESR) based on EULAR criteria and relative DAS28 values (rDAS28) and all adverse drug events were recorded. Patients were genotyped for selected polymorphism by PCR-RFLP method. According to the EULAR response criteria after 6 months of MTX therapy 146 (79.3%) patients were classified as responders, (17 patients (11.6%) were good and 129 patients (88.4%) were moderate responders) and 38 patients (20.7%) as non-responders. ADEs were observed in 53 (28.8%) patients. The majority of ADEs were mild (36 (19.56%) patients) to moderate (12 (6.25%) patients). Five patients (2.7%) had serious ADEs. Association studies have been conducted between obtained genotypes and the efficacy and toxicity of MTX. We have observed no association between polymorphisms and efficacy or toxicity of MTX in RA patients.

Key words: genetic polymorphism, methotrexate, MTHFR, MTHFD1, RFC1, rheumatoid arthritis

Corresponding author: Dubravka Vejnovic, Svetog Save 31/8, 11000 Belgrade, Serbia,Tel: +381642055073; 381607040197,Email: dubravka.vejnovic@gmail.com

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Published in „GENETIKA“ Vol. 48, No.1(2016), pp. 409-421

Serbia

UDC 575

DOI: 10.2298/GENSR1601409J

Original scientific paper

WILMS TUMOR (WT)1 GENE EXPRESSION IN CHILDREN WITH ACUTE LEUKEMIA IN SERBIA

Srdja JANKOVIĆ¹, Irena MARJANOVIĆ², Nataša Tošić², Nikola KOTUR², Lidija DOKMANOVIĆ^1,3, Dejan ŠKORIĆ^1,3, Nada KRSTOVSKI^1,3, Jelena LAZIĆ^1,3,

Predrag RODIĆ^1,3, Sonja PAVLOVIĆ²,Dragana JANIĆ^1,3*

¹ University Children's Hospital, Belgrade, Serbia

² Institute of Molecular Genetics and Genetic Engineering, Belgrade, Serbia

³ University of Belgrade School of Medicine, Belgrade, Serbia

Abstract

Janković S., I. Marjanović, N. Tošić, N. Kotur, L. Dokmanović, D. Škorić, N. Krstovski, J. Lazić, P. Rodić, S.Pavlović,D. Janić (2016): Wilms tumor (wt)1 gene expression in children with acute leukemia in Serbia- Genetika, Vol 48, No.1,409 -421.

Acute leukemias constitute the most common malignancy in childhood, accounting for 25-35% of all cancer in children. They are divided into acute lymphoblastic leukemia (ALL) and acute myeloid leukemia (AML). Genetic susceptibility is known to play a major role in childhood leukemias. Wilms tumor (WT)1 is a zinc finger transcription factor involved in regulating the process of cell differentiation; it has been implicated in a wide range of human neoplasms. WT1 overexpression in the bone marrow at diagnosis is reported to be an independent negative prognostic factor in adults and children with AML. The aim of the present investigation was to determine the expression of WT1 in the bone marrow of children with AML and ALL in Serbia and its possible impact on patient survival. We determined bone marrow WT1 expression levels by reverse-transcription polymerase chain reaction (RT-PCR) at diagnosis in 20 children with AML and 20 children with ALL (16 B-ALL and 4 T-ALL), as well as 15 age- and sex-matched controls who were evaluated for immune thrombocytopenic purpura (ITP). For children with AML, follow-up samples were also analyzed one month after treatment initiation and at variable later timepoints of control punctures. The results were normalized based on WT1 expression in controls. We found that children with AML had significantly higher WT1 expression at diagnosis (median ± SD: 139.42 ± 244.03) than those with ALL (1.18 ± 54.37; Mann-Whitney U=82; p<0.01) and ITP (0.76 ± 1.01; U=32; p<0.01). Patients with T-ALL had higher WT1 expression than those with B-ALL, though significance was not reached due to subgroup size; differences between AML subgroups according to the French-American-British (FAB) classification were also below the level of significance, though a tendency toward higher values in M3 and M4 leukemias was notable. There was also a tendency toward higher values in 14 children with AML who were still alive after a median follow-up of 1.5 years (181.42 ± 192.52) than in 6 who succumbed to the disease (104.29 ± 354.87). All children with AML who had WT1 expression 1 month after diagnosis below the fourth quartile (10 of 10) were still alive, while only 2 of 5 with 1-month WT1 expression in the fourth quartile survived (Fisher’s exact test: p=0.0952). Taken together, our results support a role for WT1 in the diagnostic workup in children with acute leukemia, although it needs to be considered in view of a complex and indvidualized context.

Key words: children, leukemia, Wilms tumor (WT)1

Corresponding author: Dragana Janić, MD, PhD,Department of Pediatric Hematology and Oncology (Head),University Children’s Hospital Tiršova 10, 11000 Belgrade, Serbia,Phone: +381 11 20 60 691,Fax: +381 11 3621413,E-mail: dragana.janic@udk.bg.ac.rs

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Published in „GENETIKA“ Vol. 48, No.1(2016), pp. 423-433

Serbia

UDC 575

DOI: 10.2298/GENSR1601423A

Original scientific paper

TRANSFORMATION, EVALUATION OF GTGENE AND MULTIVARIATE GENETIC ANALYSIS FOR MORPHO-PHYSIOLOGICAL AND YIELD ATTRIBUTING TRAITS IN Zea mays

Khadija AALIYA, Zahida QAMAR, Idrees AHMAD NASIR, Qurban ALI*, Abdul MUNIM FAROOQ and Tayyab HUSNAIN

Centre of Excellence in Molecular Biology, University of the Punjab Lahore, Pakistan

Abstract

Aaliya K., Z. Qamar, I. A. Nasir, Q. Ali, A. M. farooq and T.Husnain (2016): Transformation, evaluation of gtgene and multivariate genetic analysis for morpho-physiological and yield attributing traits in Zea mays.- Genetika, Vol 48, No.1,423 -433.

Maize is the 3^rd major crop grown all over the world that fulfills the needs of millions of people. Various biotic and abiotic factors caused reduction in grain yield of maize, among them weeds show most adverse effects. Objective of this study was to develop glyphosate resistance maize for developing maize hybrids and synthetic varieties with high grain and fodder yield potential. Glyphosate is a broad spectrum herbicide and resistance of crop against the herbicide, allows post emergence application, which otherwise is impossible. Higher GTG crude protein (479.94µg/g) was recorded for CIL 194-975 plant. The plant CIL 194-1786 showed higher expression level of GTG protein, indicated that transformation efficiency was better for line CIL-194 than CIL-123. GTGene stably integrated to the genome of the maize lines CIL-194 and CIL-123, these transformed lines showed more ability to cope with herbicides as compared with non-transformed lines in greenhouse and field trials. The transgenic lines were sown in field and data was recorded for various morpho-physiological, grain, fodder yield and quality traits. Data was statistically analyzed to check significance of results at <0.05% probability level. It was concluded from heritability, genetic advance, stepwise multiple linear regression, principle component and factor analysis that the selection on the basis of grain yield, green fodder yield, stem diameter, cob weight, stomata conductance, chlorophyll contents, photosynthetic rate, cob length, grain protein, grain oil, embryo percentage and grain starch may be fruitful keeping in view as the major contributing traits to improve crop yield and production. It was suggested that the transgenic lines for glyphosate resistance may be used to develop transgenic hybrids and synthetic varieties with higher grain yield with high quality.

Key words:glyphosate, transgenic, yield traits, Zea mays GTGene, multivariate analysis

Corresponding author: Qurban Ali*, Centre of Excellence in Molecular Biology, University of the Punjab Lahore, Pakistan, saim1692@gmail.com, qurban.ali@cemb.edu.pk

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