GENETIKA, Vol. 47, No. 1 (2015)
Dragoslav MARINKOVIĆ
In memoriam prof dr Nikola Tucić (1946-2015)
Aleksandar MIKIĆ, Aleksandar MEDOVIĆ, Živko
JOVANOVIĆ, and Nemanja STANISAVLJEVIĆ
A NOTE ON THE EARLIEST DISTRIBUTION,
CULTIVATION AND GENETIC CHANGES IN BITTER VETCH (Vicia ervilia) IN
ANCIENT EUROPE [Abstract] [Full
text]
Hasan PINAR, Sezai ERCISLI, Mustafa UNLU, Mustafa BIRCAN, Aydın UZUN, Davut KELES, Filiz BAYSAL, Halit Seyfettin ATLI, Kadir Ugurtan YILMAZ
DETERMINATION OF GENETIC DIVERSITY AMONG SOME ALMOND ACCESSIONS [Abstract] [Full text]
Radica DJEDOVIĆ, Vladan BOGDANOVIĆ, Predrag PERIŠIĆ, Dragan STANOJEVIĆ, Jelena POPOVIĆ, Muhamed BRKA
RELATIONSHIP BETWEEN GENETIC POLYMORPHISM
OF Κ- CASEIN AND QUANTITATIVE MILK YIELD TRAITS IN CATTLE BREEDS AND
CROSSBREDS IN SERBIA [Abstract]
[Full
text]
Aleksandar MARKOVSKI and Lence VELKOSKA-MARKOVSKA
INVESTIGATION OF THE MORPHOMETRIC CHARACTERISTICS OF JUJUBE TYPES (Zizyphus jujuba Mill.) FRUITS IN REPUBLIC OF MACEDONIA [Abstract] [Full text]
Azra SKENDER, Tea AJDINOVIĆ, and Dinko BEĆIRSPAHIĆ
THE COMPARISON OF PHENOTYPIC CHARACTERISTICS OF CURRENT VARIETIES AND WILD SPECIES OF Fragaria [Abstract] [Full text]
Dragana Ignjatovic-Micic, Danijela Ristic, Vojka Babic, Violeta Andjelkovic and Jelena Vancetovic
A Simple SSR
Analysis for Genetic Diversity Estimation of Maize Landraces [Abstract] [Full
text]
Ramanuj MAURYA, Umesh KUMAR, Ratna KATIYAR and Hemant KUMAR YADAV
CORRELATION AND PATH COEFFICIENT ANALYSIS IN Jatropha
curcas L. [Abstract] [Full
text]
Snežana MILOŠEVIĆ, Milena LOJIĆ, Dragana ANTONIĆ, Aleksandar CINGEL, Angelina SUBOTIĆ
CHANGES OF ANTIOXIDATIVE ENZYMES IN Impatiens walleriana L. SHOOTS IN RESPONSE TO GENETIC TRANSFORMATION [Abstract] [Full text]
Umakanta SARKER, Tofazzal ISLAM, Golam RABBANI and Shinya OBA
GENOTYPE
VARIABILITY IN COMPOSITION OF ANTIOXIDANT VITAMINS AND MINERALS IN VEGETABLE
AMARANTH [Abstract] [Full
text]
Milan BRANKOV, Milena SIMIĆ, Vesna DRAGIČEVIĆ, Sava VRBNIČANIN, and Igor SPASOJEVIĆ
GENOTYPE DEPENDENT TOLERANCE TO HERBICIDES OF
MAIZE ( Zea mays,
L) INBRED LINES [Abstract] [Full
text]
Ezatollah FARSHADFAR and Reza AMIRI
GENETIC ANALYSIS OF PHYSIOLOGICAL
INDICATORS OF DROUGHT TOLERANCE IN BREAD WHEAT USING DIALLEL TECHNIQUE [Abstract] [Full text]
Ana MILOSEVIC-DJERIC, Susanne MÜLLER, and Hans URLICH SCHAIRER
INACTIVATION OF Stigmatella aurantiaca csgA GENE IMPARES RIPPLING FORMATION [Abstract] [Full text]
Jegor MILADINOVIĆ, Miloš VIDIĆ, Vuk ĐORĐEVIĆ, and Svetlana BALEŠEVIĆ-TUBIĆ
NEW TRENDS IN PLANT BREEDING – EXAMPLE OF SOYBEAN [Abstract] [Full text]
Hongxu LONG, Xiaofeng TAN, Fangfang YAN, Lin ZHANG, Ze LI, and Heping CAO3
MOLECULAR CLONING AND EXPRESSION PROFILE OF β-KETOACYL-ACP SYNTHASE GENE FROM TUNG TREE (Vernicia fordii Hemsl.)[Abstract] [Full text]
Maryam Rezazadeh, Nasim Sohrabifar, Hossein JAFARI, Mojtaba MOHADDES ARDABILI, and Jalal Gharesouran
A STUDY ON ASSOCIATION OF POLYMORPHISMS IN Calpain10 And TCF7L2 GENES WITH TYPE 2
DIABETES MELLITUS [Abstract] [Full text]
Valentina
SEMENČENKO, Milica RADOSAVLJEVIĆ, Ljiljana MOJOVIĆ, Dušanka TERZIĆ, Marija
MILAŠINOVIĆ-ŠEREMEŠIĆ, and Goran TODOROVIĆ
A GENETIC
BASE OF UTILISATION OF MAIZE GRAIN AS A VALUABLE RENEWABLE RAW MATERIAL FOR
BIOETHANOL PRODUCTION [Abstract] [Full
text]
Gengrui ZHU, Shunli WANG, Shoumin ZHEN, Xixi SHEN,Slaven PRODANOVIC, and Yueming YAN
MOLECULAR CHARACTERIZATION AND PHYLOGENETIC ANALYSIS OF UNUSUAL X-TYPE HMW GLUTENIN SUBUNITS FROM 1SL GENOME OF Aegilops longissima[Abstract] [Full text]
Zorica ŠARAC, Jelena M. ALEKSIĆ, Tanja DODOŠ, Nemanja RAJČEVIĆ, Srdjan BOJOVIĆ, and Petar D. MARIN
CROSS-SPECIES AMPLIFICATION OF NUCLEAR EST-MICROSATELLITES DEVELOPED FOR OTHER Pinus SPECIES IN Pinus nigra [Abstract] [Full text]
Srbislav DENČIĆ, Ron DePAUW, Vojislava MOMČILOVIĆ and Ankica KONDIĆ-ŠPIKA
EFFICIENCY OF THE DIFFERENT MARKER SYSTEMS FOR ESTIMATION OF DISTINCTNESS BETWEEN SISTER LINE WHEAT CULTIVARS [Abstract] [Full text]
Ivica BUHINIČEK, Mirko JUKIĆ, Hrvoje ŠARČEVIĆ, Jerko GUNJAČA, Zdravko KOZIĆ, Antun VRAGOLOVIĆ, Ivan ŽIVKOVIĆ, Branko PALAVERŠIĆ, Ivan PEJIĆ, and Miroslav BUKAN
CHANGES OF GENETIC DIVERSITY OF MAIZE INBRED LINES OVER FOUR DECADES OF HYBRID BREEDING IN THE BC INSTITUTE REVEALED BY SSR MARKERS [Abstract] [Full text]
Gordana DANILOVIĆ, Filis MORINA, Zlatko SATOVIC, Ljiljana PROKIĆ, and Dejana PANKOVIĆ
GENETIC VARIABILITY OF Verbascum POPULATIONS FROM METAL POLLUTED AND UNPOLLUTED SITES [Abstract] [Full text]
Ezzat KARAMI, Reza TALEBI, Mehrab KHARKESH and Abbas SAIDI
A LINKAGE MAP OF CHICKPEA (Cicer arietinum L.) BASED ON POPULATION FROM ILC3279×ILC588 CROSSES: LOCATION OF GENES FOR TIME TO FLOWERING, SEED SIZE AND PLANT HEIGHT [Abstract] [Full text]
Farnaz Goodarzi, Abbas Hassani, Reza Darvishzadeh and Hamid Hatami Maleki
GENETIC VARIABILITY AND TRAITS ASSOCIATION IN CASTOR
BEAN (Ricinus communis L.) [Abstract] [Full
text]
Andrijana ANDRIĆ, Nataša KOČIŠ TUBIĆ, Milica RAT, Dragana OBREHT VIDAKOVIĆ
DIVERSITY AND GENETIC STRUCTURE OF Ornithogalum L. (Hyacinthaceae) POPULATIONS AS REVEALED BY RAPD-PCR MARKERS [Abstract] [Full text]
Ahmad DADASHPOUR, Moazzam HASSANPOUR ASIL, Reza HOJJAT-ANSARI, Behrooz ALINAGHIPOUR, and Yousef HAMIDOGHLI
STUDY ABOUT YIELD AND CUTTING’S LOSSES OF TWO TEA CLONE BUSHES IN IRAN [Abstract] [Full text]
Jovan PAVLOV, Nenad DELIĆ, Ksenija MARKOVIĆ, Miloš CREVAR, Zoran ČAMDŽIJA, and Milan STEVANOVIĆ
PATH ANALYSIS FOR MORPHOLOGICAL TRAITS IN MAIZE (Zea
mays L.) [Abstract] [Full
text]
Irena VRECAR, Aleš MAVER, Zvezdan PIRTOŠEK, Dejan GEORGIEV, Zalika KLEMENC KETIŠ, and Borut PETERLIN
FAMILY HISTORY BASED APPROACH IN RISK
PREDICTION FOR PARKINSON'S DISEASE: ADDITIONAL CONTRIBUTION OF FAMILIAL
ASSOCIATED DISORDERS[Abstract] [Full
text]
Marija RAJIČIĆ, Tanja ADNAĐEVIĆ, Gorana STAMENKOVIĆ, Jelena BLAGOJEVIĆ and Mladen VUJOŠEVIĆ
SCREENING OF B CHROMOSOMES FOR PRESENCE OF TWO GENES IN
YELLOW-NECKED MICE, Apodemus flavicollis (Mammalia, Rodentia) [Abstract] [Full
text]
Imen REKIK, Noureddine DRIRA, C. Douglas GRUBB, and Amine ELLEUCH
MOLECULAR CHARACTERIZATION AND EVOLUTION STUDIES OF A SERK
LIKE GENE TRANSCRIPTIONALLY INDUCED DURING SOMATIC EMBRYOGENESIS IN Phoenix
Dactylifera L v Deglet Nour [Abstract] [Full
text]
Jelena MALETIĆ, Ninoslav DJELIĆ, Milena RADAKOVIĆ, Milan MALETIĆ, Nada LAKIĆ, Vladimir KUKOLJ1, Nevenka ALEKSIĆ1, Marko ANDJELKOVIĆ and Zoran STANIMIROVIĆ
EVALUATION OF DNA DAMAGE IN RAT LYMPHOCYTES EXPOSED TO TULATHROMYCIN IN VITRO [Abstract] [Full text]
Kalidas PATI, Anilabh Das MUNSHI and Tusar Kanti BEHERA
INHERITANCE OF GYNOECISM IN CUCUMBER (Cucumis sativus L.) USING GENOTYPE GBS-1 AS GYNOECIOUS PARENT [Abstract] [Full text]
Jakub Jan SKORUPSKI
70 YEARS OF RESEARCH ON THE AMERICAN MINK (Neovison vison SCHREB., 1777) GENETICS – WHERE ARE WE NOW? [Abstract] [Full text]
Correction:
Vimaladev M and B. Kalaavathi (2014): A microarray gene expression data classification using hybrid back propagation neural network, Genetika vol46, No3, 1013-1026 [Correction]
Published in „GENETIKA“ Vol. 47, No. 1 (2015), pp. 1-11
© 2015 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
UDC 575
Review paper
A NOTE ON THE EARLIEST DISTRIBUTION, CULTIVATION AND GENETIC CHANGES IN BITTER VETCH (Vicia ervilia) IN ANCIENT EUROPE
Aleksandar MIKIĆ1*, Aleksandar MEDOVIĆ2, Živko JOVANOVIĆ3,
and Nemanja STANISAVLJEVIĆ3
1Institute of Field and Vegetable Crops, Novi Sad, Serbia
2Museum of Vojvodina, Novi Sad, Serbia
3University of Belgrade, Institute of Molecular Genetics and Genetic Engineering, Belgrade, Serbia
Abstract
Mikić A., A. Medović, Ž. Jovanović, and N. Stanisavljević (2015): A note on the earliest distribution, cultivation and genetic changes in bitter vetch (Vicia ervilia) in ancient Europe. - Genetika, Vol 47, No. 1, 1 -11.
Bitter vetch (Vicia ervilia (L.) Willd.) was a part of the everyday diet of the Eurasian Neanderthal population and the modern human Palaeolithic hunter-gatherers at the end of the last Ice Age. The major criteria to determine the domestication in bitter vetch and other ancient grain legumes are non-dehiscent pods, larger seed size and smooth seed testa. Bitter vetch seeds were found among the earliest findings of cultivated crops at the site of Tell El-Kerkh, Syria, from 10th millennium BP. Along with cereals, pea and lentil, bitter vetch has become definitely associated with the start of the 'agricultural revolution' in the Old World. Bitter vetch entered Europe in its south-east regions and progressed into its interior via Danube. Its distribution was rapid, since the available evidence reveals its presence in remote places at similar periods. Recently the first success has been obtained in the extraction of ancient DNA from charred bitter vetch seeds. The linguistic evidence supports the fact that most of Eurasian peoples have their own words denoting bitter vetch, meaning that its cultivation preceded the diversification of their own proto-languages.
Key words: archaeobotany, bitter vetch, crop domestication, crop history, paleogenetics, Vicia ervilia
Corresponding author: Aleksandar Mikić, Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad, Serbia, (phone) +381214898376 and +381648706059, (fax) +381214898377, (e-mail) aleksandar.mikic@ifvcns.ns.ac.rs, aleksandar.mikich@gmail.com
Published in „GENETIKA“ Vol. 47, No. 1 (2015), pp. 13 -22
© 2015 Serbian
Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:630
Original scientific paper
DETERMINATION OF GENETIC DIVERSITY AMONG SOME ALMOND ACCESSIONS
Hasan PINAR1, Sezai ERCISLI2, Mustafa UNLU1, Mustafa BIRCAN1, Aydın UZUN3,
Davut KELES1, Filiz BAYSAL1, Halit Seyfettin ATLI4, Kadir Ugurtan YILMAZ3
1Alata Horticultural Research Station- Erdemli-Mersin,Turkey
2Ataturk University, Departmant of Horticulture-Erzurum,Turkey
3 Erciyes University, Departmant of Horticulture-Kayseri,Turkey
4 Siirt University, Departmant of Horticulture-Siirt,Turkey
Abstract
Pinar H., S. Ercisli, M. Unlu, M. Bircan, A. Uzun, D. Keles, .F. Baysal, H. Seyfettin Atli, and Kadir Ugurtan Yilmaz (2015): Determination of genetic diversity among some almond accessions. - Genetika, Vol 47, No. 1, 13-22.
More recently the use of different molecular markers in fruit species to determine particularly genetic diversity, genetic relationships and cultivar identification has been gained more importance. In the study, 13 randomly amplified polimorfic DNA (RAPD) and 4 inter-simple sequence repeat (ISSR) markers were used to evaluate genetic relationships among 95 almong accessions (26 foreign cultivars and 69 national cultivars and selections). The all plant material found in Almond Germplasm Repository in Gaziantep, Turkey. Both RAPD and ISSR markers distinguished the almond cultivars and selections in various levels. 17 RAPD and ISSR markers yielded a total of 73 scorable bands, which 51 are polymorphic. The two marker system exhibited variation with regard to average band sizes and polymorphism ratio. The average polymorphism was higher in ISSR (88%) compared to RAPD (74%). RAPD and ISSR marker systems were found to be useful for determining genetic diversity among almong genotypes and cultivars. Combining of two dendrograms obtained through these markers show different clustering of 96 almond specimens without geographical isolation. These results supported that almonds in Turkey indicated considerable genetic diversity.
Key words: Almond, Genetic diversity, ISSR, Molecular characterization, Prunus dulcis,
Corresponding author: Sezai Ercisli, Ataturk University Agricultural Faculty Department of Horticulture 25240 Erzurum-Turkey, Phone: 090 4422312599, Fax: 090 4422360958, e-mail: sercisli@gmail.com
Published in „GENETIKA“ Vol. 47, No. 1 (2015), pp. 23- 32
© 2015 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575
Original scientific paper
RELATIONSHIP BETWEEN GENETIC POLYMORPHISM OF Κ- CASEIN AND QUANTITATIVE MILK YIELD TRAITS IN CATTLE BREEDS AND CROSSBREDS
IN SERBIA
Radica DJEDOVIĆ1*, Vladan BOGDANOVIĆ1, Predrag PERIŠIĆ1, Dragan STANOJEVIĆ1, Jelena POPOVIĆ2, Muhamed BRKA3
1University of Belgrade, Faculty of Agriculture, Institute for zootechniques, Belgrade,Serbia
2Institute of molecular genetics and genetic engineering, Belgrade,Serbia
3University of Sarajevo, Faculty of Agriculture and Food Sciences, Institute for zootechniques, Sarajevo, BIH
Abstract
Djedović R., V. Bogdanović, P. Perišić, D. Stanojević, J. Popović, M. Brka (2015): Relationship between genetic polymorphism of κ- casein and quantitative milk yield traits in cattle breeds and crossbreds in Serbia. - Genetika, Vol 47, No. 1, 23- 32.
The quality and yield of milk, and therefore of dairy products as well, depends directly on their protein composition. Milk gene proteins represent structural locuses that are functionally associated with milk production. In cattle selection a special importance is attached to the variability of kappa casein (κ-CN) as one of the four casein milk proteins. Genotypes of 21 Simmental breed cows, 20 crossbred cows obtained by crossing of Simmental and Red Holstein breed, and 25 cows of autochthonous Busha breed were determined for kappa casein by restriction fragment length polymorphism analysis (PCR-RFLP) of amplified DNA.
The κ-CN genotype frequencies for Simmental breed were: 42.8; 47.6 and 9.6 % for AA, AB and BB genotype, for crossbreds: 75.0; 25.0 and 0.0% and for Busha individuals: 41.7; 50.0 and 8.3 %, respectively. Frequencies of A and B alleles, assessed on the basis of genotypic frequencies were 0.667 and 0.333 for Simmental breed, 0.875 and 0.125 for crossbreds and 0.667 and 0.333 for autochthonous Busha cattle breed, respectively. For confirming the phenotypic variability of milk yield in standard lactation, yield of milk fat and the content of milk fat per κ-CN genotypes within breeds observed the fixed model which included the breed effect, lactation in order and effect of κ-CN genotype (AA, AB and BB) was used. The κ-CN genotype statistically significantly (p ≤0.05) influenced milk yield and highly significantly (p ≤0.01) milk fat yield, while the content of milk fat was not statistically significantly (p >0.05) influenced in examined animals.
Key words: cattle, genetic polymorphism, κ- casein, milk traits
Corresponding author: Radica Djedović, University of Belgrade, Faculty of Agriculture, 11080 Zemun,Serbia, email:genrad@agrif.bg.ac.rs
Published in „GENETIKA“ Vol. 47, No.1 (2015), pp. 33 -43
© 2015Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:630
Original scientific paper
INVESTIGATION OF THE MORPHOMETRIC CHARACTERISTICS OF JUJUBE TYPES (Zizyphus jujuba Mill.) FRUITS IN REPUBLIC OF MACEDONIA
Aleksandar MARKOVSKI1* and Lence VELKOSKA-MARKOVSKA2
1 Institute of Agriculture, “Ss. Cyril and Methodius” University in Skopje,
Skopje, Macedonia
2 Faculty of Agricultural Sciences and Food, “Ss. Cyril and Methodius”
University in Skopje, Skopje,Macedonia
Abstract
Markovski A. and L. Velkoska-Markovska (2015): Investigation of the morphometric characteristics of jujube types (Zizyphus jujuba mill.) fruits in Republic of Macedonia-. Genetika, Vol 47, No. 1, 33 - 43.
In the period 2002-2004 have been studied 131 Jujube types in the experimental orchards of Agricultural Institute in Skopje. They are progeny derived from six varieties of Jujube: Zu tao czao, Da baj czao, Kitajski 2A, Wild midleasiatic jujube type, Ja czao, Vahshski 45-2. The seeds of these varieties were obtained by open pollination. Studies were done on the 7-9 aged plants in full fertility. Characteristics of fruits, fruit dimensions and mass of fruit were determined. It was established that the variety Kitajski 2A has the largest fruits (13.1 g). The progeny of the same variety (Kitajski 2A) is characterized with the largest fruits (8.9 g), and the progeny of the variety with the smallest fruits between varity-mothers - Da baj czao is characterized with the smallest fruits (6.3 g). The type 21/6 has an average the largest fruits (22.2 g), while the smallest are the fruits of the type 16/4 (1.8 g). The fruits from type 21/6 are bigger than fruits of the biggest Jujube variety introduced in R Macedonia -Ta jan czao.
Key words: jujube, fruit, morphometric characteristics, open pollination, variety, types
Corresponding author: Aleksandar Markovski, Institute of Agriculture, “Ss. Cyril and Methodius” University in Skopje, 16-th Macedonian brigade 3A, 1000 Skopje, Macedonia, Tel fax: +389 2 323 0910, email: maraleks@yahoo.com
Published in „GENETIKA“ Vol. 47, No.1 (2015), pp. 45 -52
© 2015 Serbian
Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:630
Original scientific paper
THE COMPARISON OF PHENOTYPIC CHARACTERISTICS OF CURRENT VARIETIES AND WILD SPECIES OF Fragaria
Azra SKENDER, Tea AJDINOVIĆ, and Dinko BEĆIRSPAHIĆ
Biotehnical faculty Universityof Bihać, Bihać, Bosnia and Hercegovina
Abstract
Skender A., T. Ajdinović, and D. Bećirspahić (2015): The comparison of phenotypic characteristics of current varieties and wild species of Fragaria. -Genetika, Vol 47, No. 1, 45-52.
The aim of this study was to compare the major pomological and antioxidant properties of fruits which are comparatively studied in wild strawberry (Fragaria vesca L.), taken from natural habitats and commercially important varieties that are grown in the Una – Sana Canton (Bosnia and Herzegovina): Marmolada, Clery, Maya, Arosa and Elsanta. The aim was also to point out the potential superiority of the wild forms, how rich they are in vitamin C, phenol and anthocyanin, in order to be considered as a potential source of natural antioxidants. The results of analyzing the contents of vitamin C and the total phenols of fruit indicate that a wild strawberry has significantly higher antioxidant content of these components than all the commercial varieties tested. The content of vitamin C in fruits of a wild strawberry was 72 mg/100g and the phenol content 94.8 mg/100g. Their inclusion in breeding programs can provide significant potential to create varieties which will possess a better fruit quality with a higher content of antioxidant components. A wild strawberry is a significant genetic potential of particular importance for conservation and biodiversity of certain areas, as well as for the selection and breeding of cultivated fruit trees. The highest content of the total anthocyanin of fruit is registered in Marmolada (134.7 mg/100g).
Key words: strawberry, species, varieties, pomological properties, antioxidant properties.
Corresponding author: Azra Skender, Biotecnical faculty, University of Bihać, Luke Marjanovića bb, 77 000 Bihać, Bosna i Hercegovina. Telephone number: ++ 387 37 228 059; Fax number: ++ 387 37 228 059; e-mail: skenderharun@yahoo.com
Published in „GENETIKA“ Vol. 47, No. 1 (2015), pp. 53 -62
© 2015 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:633.15
Original scientific paper
A Simple SSR Analysis for Genetic Diversity Estimation
of Maize Landraces
Dragana Ignjatovic-Micic*, Danijela Ristic, Vojka Babic, Violeta Andjelkovic
and Jelena Vancetovic
Maize Research Institute Zemun Polje, Belgrade, Serbia
Abstract
Ignjatovic-Micic D., D. Ristic, V. Babic, V. Andjelkovic and J. Vancetovic (2015): A simple SSR analysis for genetic diversity estimation of maize landraces.-Genetika, Vol 47, No. 1, 53-62.
A collection of 2217 landraces from western Balkan (former Yugoslavia) is maintained at Maize Research Institute Zemun Polje gene bank. Nine flint and nine dent accessions from six agro-ecological groups (races), chosen on the basis of diverse pedigrees, were analyzed for genetic relatedness using phenotypic and simple sequence repeat (SSR) markers. One of the aims was to establish a reliable set of SSR markers for a rapid diversity analysis using polyacrilamide gels and ethidium bromide staining. In the principal component analysis (PCA) the first three principal components accounted for 80.86% of total variation and separated most of the flint from dent landraces. Ten SSR primers revealed a total of 56 and 63 alleles in flint and dent landraces, respectively, with low stuttering and good allele resolution on the gels. High average PIC value (0.822) also supports informativeness and utility of the markers used in this study. Higher genetic variation was observed among flint genotypes, as genetic distances between flint landraces covered a larger range of values (0.11- 0.38) than between dent (0.22 – 0.33) genotypes. Both phenotypic and SSR analyses distinguished flint and dent landraces, but neither of them could abstract agro-ecological groups. The SSR method used gave clear, easy to read band patterns that could be used for reliable allele frequency determination. Genetic diversity revealed for both markers indicated that the landraces were highly adapted to specific environmental conditions and purposes and could be valuable sources of genetic variability.
Key words: accessions, genetic diversity, phenotype, SSR, Zea mays L.
Corresponding author: Dragana Ignjatovic-Micic, Maize Research Institute Zemun Polje, Slobodana Bajica 1, Belgrade, Serbia, phone: +381113756704, fax: +381113756707, e-mail: idragana@mrizp.rs
Published in „GENETIKA“ Vol. 47, No. 1 (2015), pp.63 -70
© 2015 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.630
Original scientific paper
CORRELATION AND PATH COEFFICIENT ANALYSIS IN Jatropha curcas L.
Ramanuj MAURYA1, Umesh KUMAR1 , Ratna KATIYAR2 and Hemant KUMAR YADAV1,3*
1 CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow-226001, India
2 Department of Botany, Lucknow University, Lucknow, India
3 Academy of Scientific and Innovative Research (AcSIR), New Delhi, India
Abstract
Maurya R., U. Kumar, R. Katiyar and H. Kumar Yadav (2015): Correlation and path coefficient analysis in Jatropha curcas L.. - Genetika, Vol 47, No. 1, 63-70.
Correlation and path analysis on 80 diverse accessions of J. curcas showed that seed weight/plant was significantly and positively associated with female flower/plant, male flower/plant, number of flower/plant, number of seed/plant, fruit weight/plant, seed width and negatively associated with oil content. Oil content was negatively and significantly correlated with all the traits studied with strong negative association with female flower/plant followed by male flower/plant, number of seed/plant, fruit weight/plant and seed weight/plant. Male flower per plant had the maximum direct effect on seed yield, followed by number of seeds/plant, seed width, number of fruits/plant and oil content. The results of the present investigations suggests that selection in J. curcas based on male flower/plant, number of fruit/plant, number of seeds/plant, seed width and oil content would be advantageous to achieve the desirable goals. The indirect selection through other component traits would also be rewarding to improve the seed yield.
Key words: Jatropha curcas, correlation, path coefficient analysis, breeding, selection
Corresponding author: Hemant Kumar Yadav, Academy of Scientific and Innovative Research (AcSIR), New Delhi, India, Tel.: +91-522-2297938, Fax: +91-522-2205836,,email address: h.yadav@nbri.res.in
Published in „GENETIKA“ Vol. 47, No.1 (2015), pp. 71-84
© 2015 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:630
Original scientific paper
CHANGES OF ANTIOXIDATIVE ENZYMES IN Impatiens walleriana L. SHOOTS
IN RESPONSE TO GENETIC TRANSFORMATION
Snežana MILOŠEVIĆ*, Milena LOJIĆ, Dragana ANTONIĆ, Aleksandar CINGEL,
Angelina SUBOTIĆ
Institute for Biological Research, University of Belgrade, Belgrade, Serbia
Abstract
Milošević S., M. Lojić, D. Antonić, A. Cingel, and A. Subotić (2015): Changes of antioxidative enzymes in Impatiens walleriana L. shoots in response to genetic transformation. - Genetika, Vol 47, No. 1, 71- 84.
Impatiens walleriana L. shoots were inoculated with Agrobacterium rhizogenes A4M70GUS and the effects of genetic transformation on the catalase (CAT), superoxide dismutase (SOD) and peroxidase (POX) activities in wounded region of stems and unwounded leaves were evaluated 10, 24, 240 and 720 hours after inoculation. Following Agrobacterum infection activities of plant antioxidative enzymes changed in a time-dependent manner indicating that dynamic processes occurred during plant-Agrobacterium interaction, plant cell transformation and formation of hairy roots. Appearance of hairy roots on wound sites of shoots was observed ten days after inoculation with A. rhizogenes and the root induction frequency was 100%. Among selected hairy root lines significant differences in growth rate and biomass production were observed and an average 3-fold increase in biomass production was observed for the best growing hairy root line compared with the untransformed roots. PCR analysis showed presence of uidA, rolB, rolC and rolD genes in all analyzed I. walleriana L. hairy root lines, while amplification fragment of rolA gene was detected in 83.3% transformed lines. Efficient transformation protocol for I. walleriana L described in this work offer possibilities to generate hairy root cultures for in vitro propagation of plant viruses.
Key words: Agrobacterium rhizogenes; antioxidative enzymes; Impatiens walleriana L.
Corresponding author: Snežana Milošević, Institute for Biological Research, University of Belgrade, Bul. despota Stefana 142, 11060 Belgrade, Serbia, tel: + 381 11 2078393, fax: + 381 11 2761433, e-mail: snezana@ibiss.bg.ac.rs
Published in „GENETIKA“ Vol. 47, No 1 (2015), pp. 85-96
© 2015 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:630
Original scientific paper
GENOTYPE VARIABILITY IN COMPOSITION OF ANTIOXIDANT VITAMINS AND MINERALS IN VEGETABLE AMARANTH
Umakanta SARKER1, Tofazzal ISLAM2, Golam RABBANI3 and Shinya OBA4
1Department of Genetics and Plant Breeding, Faculty of Agriculture, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh
2Department of Biotechnology, Faculty of Agriculture, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh
3Department of Horticulture, Faculty of Agriculture, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
4Laboratory of Field Science, Faculty of Applied Biological Science, Gifu University, Gifu, Japan
Abstract
Sarker U., T. Islam, G. Rabbani and S. Oba (2015): Genotype variability in composition of antioxidant vitamins and minerals in vegetable amaranth. - Genetika, Vol 47, No. 1, 85-96.
Twenty five vegetable amaranth genotypes were evaluated to investigate the antioxidant vitamins and minerals composition and its genetic variability in a RCBD with three replications at Bangabandhu Sheikh Mujibur Rahman Agricultural University in Bangladesh during kharif1 season 2012 and 2013, respectively. Significant Mean Sum of Square revealed a wide range of genotypic variability among traits. Vegetable amaranth was rich in iron, zinc, manganese, magnesium and potassium. Ten strains gave the best (more than 5 kg) foliage yield with rich in antioxidant minerals and vitamins. Selection of these genotypes would be economically useful for antioxidant vitamins, minerals and yield aspects. On the other hand, eight genotypes had high amounts of antioxidant vitamins and minerals with below average foliage yield and could be utilized as donor parents for introgression of genes in vitamins and minerals deficient lines. Considering genetic parameter six traits i e., Fe, Zn, Mn, ascorbic acid, number of leaves plant and foliage yield would be selected for the improvement of vegetable amaranth genotypes under study. However, correlation study revealed that selection based on Fe, Mn, ascorbic acid and number of leaves per plant could lead to increase the foliage yield of vegetable amaranth strains. Insignificant genotypic correlations between foliage yield with most of the antioxidant vitamins and minerals traits indicating that selection for high vitamins and minerals content might be possible without compromising yield loss. Based on mean, genetic parameters and correlation coefficient values, five vegetable amaranth genotypes i. e., AA19, AA10, AA3, AA24 and AA7 might be selected as high vitamin and minerals containing high yielding vegetable amaranth varieties.
Key words: foliage yield, antioxidant, ascorbic acid, beta-carotene, mineral content
Corresponding author: Umakanta Sarker, Department of Genetics and Plant Breeding, Faculty of Agriculture, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh E-mail: umakanta_sarker@yahoo.com
Published in „GENETIKA“ Vol. 47 No. 1 (2015), pp. 97 - 106
© 2015 Serbian
Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:633.15
Original scientific paper
GENOTYPE DEPENDENT TOLERANCE TO HERBICIDES OF MAIZE ( Zea mays, L) INBRED LINES
Milan BRANKOV1, Milena SIMIĆ1, Vesna DRAGIČEVIĆ1, Sava VRBNIČANIN2,
And Igor SPASOJEVIĆ1
1 Maize Research Institute „Zemun Polje“, Belgrade, Serbia
2 University of Belgrade, Faculty of Agriculture, Serbia
Abstract
Brankov M., M. Simić, V. Dragičević, S. Vrbničanin, and I. Spasojević (2015): Genotype dependent tolerance to herbicides of maize (Zea mays, L) inbred lines- Genetika, Vol 47, No. 1, 97-106
Herbicide application in seed maize requires special attention due to their larger sensitivity than hybrid maize. The aim of study was to examine and define the sensitivity/tolerance of the five maize inbred lines with different susceptibility to herbicides (belonging to different heterotic groups), based on alterations of secondary metabolites (phenolics, protein sulfhydryl groups, phytic and inorganic phosphorus). Two groups of herbicides: triketons (mesotrione and topramezone) and sulfonylureas (rimsulfuron and foramsulfuron) were tested. Lines from independent heterotic group, which were sensitive to herbicides expressed visible damages together with significant reduce in grain yield, mainly induced by sulfonylurea herbicides. Parallel with that, significant increase in phenolics, phytic and inorganic phosphorus, as well as drop in protein sulfhydryl groups were observed in their leaves. Tolerant lines (belonging to Lancaster group) had mainly insignificant grain yield reduce, also with lesser variations in sulfhydryl groups, content of phytic and inorganic phosphorus, as well as increase in phenolics content. Among examined secondary metabolites, phytate is the main factor, contributing to herbicide tolerance in maize lines. Owing to lesser yield decrease and variation in content of examined secondary metabolites, expressed in treatments with triketone herbicides, they usage could be safe in maize lines.
Key words: herbicides, maize lines, secondary metabolites, tolerance
Corresponding author: Brankov Milan, Maize Research Institute „Zemun Polje“, Belgrade, Serbia, Phone +381113756704, Fax: +381113756707,e-mail: mbrankov@mrizp.rs
Published in „GENETIKA“ Vol. 47, No. 1(2015), pp. 107- 118
© 2015 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:633.11
Original scientific paper
GENETIC ANALYSIS OF PHYSIOLOGICAL INDICATORS OF DROUGHT TOLERANCE IN BREAD WHEAT USING DIALLEL TECHNIQUE
Ezatollah FARSHADFAR1, 2* and Reza AMIRI3
1Campus of Agriculture and Natural Resources, Razi University, Kermanshah, Iran
2Department of Agronomy and Plant Breeding, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran
3 Campus of Agriculture and Natural Resources, Razi University, Kermanshah, Iran
University of Medical Sciences, Tabriz, Iran
Abstract
Farshadfar E.and, R. Amiri (2015): Genetic analysis of physiological indicators of drought tolerance in bread wheat using diallel technique.- Genetika, Vol 47, No. 1,107 -118.
In order to study genetic architecture of physiological criteria of drought tolerance in wheat using different diallel techniques, an experiment was conducted on six bread wheat genotypes as parents and their 15 hybrids in a randomized complete block design with three replicates under rainfed conditions at the Research Farm of the Campus of Agriculture and Natural Resources, Razi University, Kermanshah, Iran during 2010-2011 cropping season. The results of analysis of variance showed significant differences between the genotypes for relative water content (RWC), relative chlorophyll content (RCC), chlorophyll a (Chl a), chlorophyll b (Chl b), total chlorophyll (Chl T) and proline concentration (PC). RWC, Chl T and PC exhibited significant differences for general combining ability, indicating the involvement of additive gene action in their inheritance. Moreover, as specific combining ability was not significant for all studied traits, hence these traits are predominantly controlled by additive gene action. Parent number one was the best general combiner for improvement of RWC, RCC and Chl b, while the best general combiner for improvement of Chl a, Chl T and PC was parent number five. Also, the best specific combination for improvement of RWC, RCC, Chl a, Chl b, Chl T and PC were the crosses 2×4, 1×2, 3×6, 1×6, 1×6 and 1×4, respectively indicating that parents of these crosses are genetically varied. Hayman and Morley-Jones analysis of variance revealed that the inheritance of RWC, Chl a, Chl b, and Chl T was mainly controlled by additive gene effects, while PC was controlled by both additive as well as dominance type of gene action.
Key words: Combining ability, genetic analysis, gene action, heritability
Corresponding author: Ezatollah Farshadfar, Professor of Plant Breeding, Department of Agronomy and Plant Breeding, Campus of Agriculture and Natural Resources, Razi University, Kermanshah, Iran, 6715685438., Tel: +98 8330111; Mobile No: +98 9183893472; Fax No: +98 8338323731,E-mail: e_farshadfar@yahoo.com
Published in „GENETIKA“ Vol. 47, No.1 (2015), pp. 119 -130
© 2015Serbian
Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575
Original scientific paper
INACTIVATION OF Stigmatella aurantiaca csgA GENE IMPARES RIPPLING FORMATION
Ana MILOSEVIC-DJERIC1, Susanne MÜLLER2, and Hans URLICH SCHAIRER3
1 Department of Gynaecology and Obstetrics, Hospital Centre Uzice, Uzice, Serbia
2 Univeristy of Iowa, Iowa City, USA
3Zentrum fur Molekulare Biologie, ZMBH, Heidelberg, Germany
Abstract
Milosevic-Djeric A., S. Müller, and H. Urlich Schairer (2015): Inactivation of Stigmatella aurantiaca csga gene impares rippling formation- Genetika, Vol 47, No. 1, 119-130.
Stigmatella aurantiaca fruiting body development depends on cell-cell interactions. One type of the signaling molecule stigmolone isolated from S. aurantiaca cells acts to help cells to stay together in the aggregation phase. Another gene product involved in intercellular signaling in S. aurantiaca is the csgA homolog of Myxococcus xanthus.
In close relative M. xanthus C signal the product of the csgA gene is required for rippling, aggregation and sporulation. Isolation of homologous gene in S. aurantiaca implicates a probable role of CsgA in intercellular communication. Inactivation of the gene by insertion mutagenesis caused alterations in S. aurantiaca fruiting. The motility behavior of the cells during development was changed as well as their ability to stay more closely together in the early stages of development. Inactivation of the csgA gene completely abolished rippling of the cells. This indicates the crucial role of the CsgA protein in regulating this rhythmic behavior.
Key words: Stigmatella aurantiaca; cell-cell signaling; fruiting body formation; development, myxobacteria.
Corresponding author: Ana Milosevic-Djeric, Department of Gynecology and Obstetrics, Hospital Uzice, Milosa Obrenovica 17, 31 000, tel +38131 561255, Uzice, Serbia, mail: ana.mdjeric@gmail.com.
Published in „GENETIKA“ Vol. 47, No. 1(2015), pp. 131-142
© 2015Serbian
Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575.633
Original scientific paper
NEW TRENDS IN PLANT BREEDING – EXAMPLE OF SOYBEAN
Jegor MILADINOVIĆ, Miloš VIDIĆ, Vuk ĐORĐEVIĆ, and Svetlana BALEŠEVIĆ-TUBIĆ
Institute of Field and Vegetable Crops, Novi Sad, Serbia
Abstract
Miladinović J., M. Vidić, V. Đorđević, and S. Balešević-Tubić (2015): New trends in plant breeding – example of soybean.- Genetika, Vol 47, No. 1, 131-142.
Soybean breeding and selection is a continual process designed to increase yield levels and improve resistance to biotic and abiotic stresses. Soybean breeders have been successful in producing a large number of varieties using conventional breeding methods, the Single Seed Descent method in particular. In recent decades, with the increased use of genetic transformations, backcrossing is more frequent though the only trait that has been commercialized is glyphosate tolerance. Physiological breeding poses a particular challenge, as well as phenotyping and development of useful criteria and techniques suitable for plant breeding. Using modern remote sensing techniques provides great opportunity for collecting a large amount of physiological data in real environment, which is necessary for physiological breeding. Molecular based plant breeding methods and techniques are a conceptual part of any serious breeding program. Among those methods, the most extensively used is marker-assisted selection, as a supplement to conventional breeding methods.
Key words: breeding, methods soybean, crop phisiology molecularmarkers.
Corresponding author: Jegor Miladinović, Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad, Serbia; Phone: +381214898110, Fax: +3814898131;E-mail: jegor.miladinovic@nsseme.com
Published in „GENETIKA“ Vol. 47, No.1(2015), pp. 143- 159
© 2015 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:630
Original scientific paper
MOLECULAR CLONING AND EXPRESSION PROFILE OF β-KETOACYL-ACP SYNTHASE GENE FROM TUNG TREE (Vernicia fordii Hemsl.)
Hongxu LONG 1, 2, Xiaofeng TAN 1, 2*, Fangfang YAN 1, 2, Lin ZHANG 1, 2,
Ze LI 1, 2, and Heping CAO 3
1 Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha, China
2 Hunan Provincial Cooperative Innovation Center of Non-wood Forest Cultivation and Utilization, Central South University of Forestry and Technology, Changsha, China
3 U.S. Department of Agriculture, Agricultural Research Service, Southern Regional Research Center, Commodity Utilization Research Unit, New Orleans, Louisiana, USA
Abstract
Long H., X. Tan, F. Yan, L.Zhang, Z. Li, and H. Cao (2015): Molecular cloning and expression profile of β-ketoacyl-acp synthase gene from tung tree (Vernicia fordii Hemsl.) - Genetika, Vol 47, No. 1, 143-159.
Tung tree (Vernicia fordii) is an important woody oil tree. Tung tree seeds contain 50-60% oil with approximately 80 mole α-eleostearic acid (9 cis, 11 trans, 13 trans octadecatrienoic acid). Fatty acid synthesis is catalyzed by the concerted action of acetyl-CoA carboxylase and fatty acid synthase, a multienzyme complex including β-ketoacyl-acyl-carrier-protein synthase (KAS). Little is known about KAS in tung tree. The objective of this study was to clone KAS genes and analyze their expression profiles in tung tree. A full-length cDNA encoding KAS III and a partial cDNA encoding KAS II were isolated from tung tree by PCR cloning using degenerate primers and rapid amplification of cDNA ends system. The full-length cDNA of VfKAS III was 1881 bp in length with an open reading frame of 1212 bp. VfKAS III genomic DNA was also isolated and sequenced, which contained 8 exons in 5403 bp length. The deduced VfKAS III protein shared approximately 80% identity with homologous KAS IIIs from other plants. Quantitative PCR analysis revealed that KAS II and KAS III were expressed in all of the tissues and organs tested but exhibited different expression patterns in tung tree. The expression levels of KAS II in young tissues were much lower than those in mature tissues, whereas the highest expression levels of KAS III were observed in young stem and young leaf. These results should facilitate further studies on the regulation of tung oil biosynthesis by KAS in tung tree.
Key words: β-ketoacyl-ACP synthase, expression profile, molecular cloning, Vernicia fordii
Corresponding author: Xiaofeng Tan, College of forestry, Central South University of Forestry and Technology, Changsha, 410004, China. Tel: +86-0731-85623416; Fax: +86-0731-85623406; E-Mail: tanxiaofengcn@126.com
Published in „GENETIKA“ Vol. 47, No. 1(2015), pp. 161- 170
© 2015 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575
Original scientific paper
A STUDY ON ASSOCIATION OF POLYMORPHISMS IN Calpain10 And TCF7L2 GENES WITH TYPE 2 DIABETES MELLITUS
Maryam Rezazadeh1,2, Nasim Sohrabifar1, Hossein JAFARI1, Mojtaba MOHADDES ARDABILI1, and Jalal Gharesouran1
1Department of Medical Genetics, Faculty of Medicine, Tabriz University of Medical Sciences, Iran
2Genetics Research Center, University of Social Welfare and Rehabilitation Sciences,
Tehran, Iran
Abstract
Rezazadeh M., N. Sohrabifar, H. Jafari, M. Mohaddes Ardabili, and J. Gharesouran (2015): A study on association of polymorphisms in calpain10 and tcf7l2 genes with type 2 Diabetes mellitus.- Genetika, Vol 47, No. 1, 161-170
Polymorphisms of the Calpain10 and TCF7L2 genes were identified as possible type 2 diabetes susceptibility genetic markers. We conducted a case-control study to evaluate the relation between SNP43 of calpain-10 and rs12255372 and rs7903146 in the TCF7L2 with type2 diabetes in western–north of Iran. The role of these variants in Iranian population was less clear. A total of 202 patients and healthy controls were enrolled to analysis the frequency distribution of Calpain10 and TCF7L2 polymorphisms (SNP43, rs12255372 and rs7903146) using polymerase chain reaction-restriction fragment length polymorphism (PCR – RFLP) method. The frequency of allele A in controls was significantly greater than that of diabetic patients (P=0.031), whereas the difference between distribution of SNP43 genotypes (A/A, A/G, G/G) were non-significant in case and control groups. Non significant association was also observed between G/G, A/G or A/A genotypes and type 2 Diabetes. The frequency of the “T” allele of rs12255372 (G/T) was significantly associated with type 2 diabetes (OR= 0.55, 95% confidence interval [CI], 1.11-1.51; P<0.001). No allelic association was found for rs7903146(C/T) polymorphism.The distribution of alleles in case and control groups are significantly different indicating the G allele is associated with type 2 diabetes. The rs12255372 (G/T) may be associated with type 2 diabetes.
Key words: Calpain10, SNP43, Type 2 Diabetes, Eastern Azerbaijan, Iran
Corresponding author: Jalal Gharesouran, Department of Medical Genetics, Faculty of Medicine, Golghasht St, Tabriz University of Medical Sciences, Tabriz, Iran, Tel/Fax: ++98-41-33371587,E-mail: Gharesouranj@tbzmed.ac.ir
Published in „GENETIKA“ Vol. 47 No. 1(2015), pp. 171- 184
© 2015 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:630.15
Original scientific paper
A GENETIC BASE OF UTILISATION OF MAIZE GRAIN AS A VALUABLE RENEWABLE RAW MATERIAL FOR BIOETHANOL PRODUCTION
Valentina SEMENČENKO1, Milica RADOSAVLJEVIĆ1, Ljiljana MOJOVIĆ2,
Dušanka TERZIĆ1, Marija MILAŠINOVIĆ-ŠEREMEŠIĆ1, and Goran TODOROVIĆ1
1Maize Research Institute, Zemun Polje, Belgrade-Zemun, Serbia
2Faculty of Technology and Metallurgy, University of Belgrade, Belgrade, Serbia
Abstract
Semenčenko V., M. Radosavljević, Lj. Mojović, D. Terzić, M. Milašinović-Šeremešić, and G. Todorović (2015): A genetic base of utilisation of maize grain as a valuable renewable raw material for bioethanol production - Genetika, Vol 47 No. 1, 171-184
Maize (Zea mays L.) is one of the most important cereal crops, and as such, one of the most significant naturally renewable carbohydrate raw materials for the production of energy and multitude of different products. Many studies have shown that the kernel composition and starch structure of maize are highly influenced by genetic background of the maize. Maize grain consists of approximately 70% of starch, which makes it a very suitable feedstock for the bioethanol production. This study was conducted with aim to understand how different genetic background affects bioethanol yield and other fermentation properties of the selected maize genotypes in the process of maize grain-based bioethanol production. Twenty seven maize hybrids, including genotypes of standard chemical composition as well as specialty maize hybrids such as popping, waxy, white kernel and red kernel hybrids, developed at the Maize Research Institute, Zemun Polje, were investigated in this study. The lowest bioethanol yield of 7.25% w/w obtained for hybrid ZP 611k after 48 h of fermentation and the highest by genotype ZP 434 (8.96% w/w). A very significant positive correlation was determined between kernel starch content and the bioethanol yield after 48h of fermentation, as well as volumetric productivity (48h) (r=0.67). Between bioethanol yield after 48h of fermentation and soft endosperm content in kernel of the investigated ZP maize hybrids a very significant positive correlation was assessed (r=0.66). Higher overall bioethanol yields have been obtained from genotypes containing higher starch and lower protein and lipid contents.
Key words: bioethanol, genetic background, maize, starch, simultaneous saccharification and fermentation (SSF)
Corresponding author: Valentina Semenčenko; Maize Research Institute, Zemun Polje, Slobodana Bajića 1, 11185 Belgrade-Zemun,Phone: +381 11 3756 704; Fax: + 381 11 3754 994; e-mail: vsemencenko@yahoo.com
Published in „GENETIKA“ Vol. 47, No. 1(2015), pp. 185-203
© 2015 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:630
Original scientific paper
MOLECULAR CHARACTERIZATION AND PHYLOGENETIC ANALYSIS OF UNUSUAL X-TYPE HMW GLUTENIN SUBUNITS FROM 1SL GENOME OF Aegilops longissima
Gengrui ZHU1, Shunli WANG1,2, Shoumin ZHEN1, Xixi SHEN1,
Slaven PRODANOVIC3*, and Yueming YAN1,4*
1College of Life Science, Capital Normal University, Beijing, China
2Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences,
Beijing, China
3Faculty of Agriculture University of Belgrade, Belgrade, Serbia
4Hubei Collaborative Innovation Center for Grain Industry, 434025 Jingzhou, China
Abstract
Zhu G., S. Wang, S. Zhen, X. Shen, S. Prodanovic, and Y. Yan (2015): Molecular characterization and phylogenetic analysis of unusual x-type HMW glutenin subunits from 1Sl genome of Aegilops longissima- Genetika, Vol 47, No 1, 185-203.
Wheat related diploid species Ae. longissima (2n=2x=14, SlSl) has extensive storage protein variations that may provide useful gene resources for wheat quality improvement. In this work, five novel 1Sl-encoded x-type high molecular glutenin subunits (HMW-GS) were identified and designated as 1Slx-123, 1Slx-129, 1Slx1-136, 1Slx2-136 and 1Slx2.2, respectively. Their complete open reading frames (ORFs) were cloned and sequenced by AS-PCR, which contained 2874 bp (956 aa) for 1Slx-123, 2946 bp (979 aa) for 1Slx-129, 2901 bp (965 aa) for 1Slx1-136, 2982bp (991 aa) for 1Slx2-136 and 2928 bp (974 aa) for 1Slx2.2. Molecular characterization demonstrated that five unusual subunits had greater repetitive domains resulted from a larger fragment insertion (74-113 aa). Particularly, 1Slx-129 had an extra cysteine residue at the position 109 due to a TAT → TGT dot mutation, which may improve the formation of superior gluten macropolymer. Our results suggest that these unusual HMW-GS could be served as potential superior gene resources for improving wheat gluten quality. Phylogenetic analysis revealed that HMW-GS genes from Glu-1Sx genomes had close evolutionary relationships with those of Glu-1Dx genome while sequences from Ae. speltoides aligned with those of B genome.
Key words: Ae. longissima, HMW-GS, molecular clone, phylogenetics
Corresponding author: Slaven Prodanovic, Faculty of Agriculture, University of Belgrade, Nemanjina 6, 11080, Belgrade, Serbia, phone: +381638687246, fax: +381113161987, e-mail: slavenp@agrif.bg.ac.rs
Yueming Yan, College of Life Science, Capital Normal University, Beijing, Xisanhuan Beilu 105, 100048 Beijing, China, phone: +861068902777, fax: +861068902777, e-mail: yanym@cnu.edu.cn
Published in „GENETIKA“ Vol. 47, No. 1(2015), pp. 205-217
© 2015 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:630
Original scientific paper
CROSS-SPECIES AMPLIFICATION OF NUCLEAR EST-MICROSATELLITES DEVELOPED FOR OTHER Pinus SPECIES IN Pinus nigra
Zorica ŠARAC1, Jelena M. ALEKSIĆ2, Tanja DODOŠ3, Nemanja RAJČEVIĆ3, Srdjan BOJOVIĆ4, and Petar D. MARIN3
1University of Niš, Faculty of Sciences and Mathematics, Department of Biology and Ecology, Niš, Serbia
2University of Belgrade, Institute of Molecular Genetics and Genetic Engineering, Belgrade, Serbia
3University of Belgrade, Faculty of Biology, Institute of Botany and Botanical Garden "Jevremovac", Belgrade, Serbia
4University of Belgrade, Institute for Biological Research "Siniša Stanković", Belgrade, Serbia
Abstract
Šarac Z., J. Aleksić, T. Dodoš, N. Rajčević, S. Bojović, and Petar Marin (2015): Cross-species amplification of nuclear EST-microsatellites developed for other Pinus species in Pinus nigra.- Genetika, Vol 47, No. 1, 205-217.
Due to the current lack of nuclear microsatellites (simple sequence repeats – SSRs) specifically developed for Pinus nigra, an important European coniferous species, we cross-species amplified 12 EST-SSRs (expressed sequence tagged SSRs) developed for other Pinus species in P. nigra in order to delineate loci which can be used for assessing levels of genetic diversity and genetic structuring in this species. We amplified these loci in individuals from seven populations from the central Balkans representing four recognized infraspecific taxa of P. nigra (ssp. nigra, var. gocensis, ssp. pallasiana, and var. banatica). Contrary to expectations on high transferability of EST-SSRs into related species, only three out of 12 tested loci were successfully amplified in P. nigra, but they displayed lack/low levels of polymorphism or generated multilocus amplification products. Thus, our estimates on levels of genetic diversity (HE = 0.183) and genetic differentiation (FST = 0.007) were based on variability of a single locus harboring four alleles only and they should be taken with cautions. Our study highlights the need for the development of high-resolution molecular markers, such as co-dominant genic or genomic SSRs or predominantly biallelic SNPs, or utilization of anonymous dominant markers, such as AFLPs, for genotyping in P. nigra.
Key words: the Balkans, Pinus nigra, EST-SSRs, cross-species amplification, genetic diversity
Corresponding author: Zorica Šarac; University of Niš; Faculty of Sciences and Mathematics; Department of Biology and Ecology; Višegradska 33; 18000 Niš; Serbia; Phone: +381-18-533-014; e-mail: saraczorica@gmail.com
Published in „GENETIKA“ Vol. 47, No. 1(2015), pp. 219-232
© 2015Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:633.11
Original scientific paper
EFFICIENCY OF THE DIFFERENT MARKER SYSTEMS FOR ESTIMATION OF DISTINCTNESS BETWEEN SISTER LINE WHEAT CULTIVARS
Srbislav DENČIĆ1,*, Ron DePAUW2, Vojislava MOMČILOVIĆ1
and Ankica KONDIĆ-ŠPIKA1
1Institute of Field and Vegetable Crops, Novi Sad, Serbia,
2Semiarid Prairie Agricultural Research Centre, AAFC, Swift Current, SK, Canada
Abstract
Denčić S., R. DePauw, V. Momčilovic and A. Kondić-Špika (2015): Efficiency of the different marker systems for estimation of distinctness between sister line wheat cultivars.- Genetika, Vol 47, No. 1, 219-232.
A set of 42 winter wheat cultivars developed from nine populations were evaluated for distinctness. Three marker systems: morphological markers, gliadin allele profiles and microsatellites were used to analyze distinctness between sister cultivars. The morphological based distinctness tests for wheat are based on a crop-specific set of characters that comply with UPOV guidelines. The morphological markers were quite informative although they were not capable to estimate the distances/distinctness between two pair of sister cultivars Evropa 90 and Evropa and Novosadska rana 3 and Novosadska rana 2. The gliadins allele profiles were the least efficient to estimate distinctness between sister line cultivars. This system was not capable to distinguish even cultivars developed from different populations. Sister line wheat cultivars were fingerprinted with 19 wheat microsatellites markers. A total of 106 alleles were detected at 19 wheat microsatellite loci, resulting in an average allele number per marker of 5.6. The number of markers was sufficient to distinguish among most sister line cultivars. Only one pair of sister cultivars, Lozničanka and Kosovka, derived from the same cross could not be distinguished. Comparing all marker systems to evaluate distinctness of sister line wheat cultivars the most efficient was microsatellite markers while gliadin allele profiles was the least efficient. Correlations between matrices based on pedigree data and morphological marker, gliadin profiles and microsatellites were significant but not large.
Key words: Distinctness, Gliadin allele profiles, Morphological markers, Microsatellite markers, Sister line cultivar, Wheat
Corresponding author: Srbislav Denčić, Institute of Field an Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad, Serbia; Phone: 381214898233; Fax: 381214898227 E-mail: srbislav.dencic@nsseme.com
Published in „GENETIKA“ Vol. 47, No. 1(2015), pp. 233-243
© 2015 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575
Original scientific paper
CHANGES OF GENETIC DIVERSITY OF MAIZE INBRED LINES OVER FOUR DECADES OF HYBRID BREEDING IN THE BC INSTITUTE REVEALED BY SSR MARKERS
Ivica BUHINIČEK1, Mirko JUKIĆ1, Hrvoje ŠARČEVIĆ2, Jerko GUNJAČA2, Zdravko KOZIĆ1, Antun VRAGOLOVIĆ1, Ivan ŽIVKOVIĆ1, Branko PALAVERŠIĆ1, Ivan PEJIĆ2, and Miroslav BUKAN1*
1Bc Institute for Breeding and Production of Field Crops, Rugvica, Dugo Selo, Croatia 2University of Zagreb, Faculty of Agriculture, Zagreb, Croatia
Abstract
Buhiniček I., M. Jukić, H. Šarčević, J. Gunjača, Z. Kozić, A. Vragolović, I. Živković, B. Palaveršić, I. Pejić, and M. Bukan (2015): Changes of genetic diversity of maize inbred lines over four decades of hybrid breeding in the Bc Institute revealed by SSR markers.- Genetika, Vol 47, No. 1, 233-243.
In this paper, changes of genetic diversity of the most important maize inbred lines used for hybrid production within the Bc Institute in the 1970s, 1980s, 1990s and 2000s were examined using the SSR markers. The average number of alleles per SSR locus was 3.14, 3.43, 3.07 and 3.25 for lines from 1970s, 1980s, 1990s and 2000s, whereas the number of private alleles for the same four decades was 8, 4, 0 and 6, respectively. Mean genetic distance among inbreds within decades steadily decreased over time from 0.64 in 1970s to 0.57 in 2000s, but the observed differences were not statistically significant. The clustering of the studied inbred lines indicates the exploitation of a known BSSS x LSC heterotic pattern within the Bc Institute maize breeding program. The overall results show that recycled inbred lines within these pools do not decline in genetic variation over the past 40 years.
Key words: genetic diversity, maize inbred lines, SSR markers
Corresponding author: Miroslav Bukan, Bc Institute for Breeding and Production of Field Crops, Rugvica, Dugoselska 7, Zagreb, Croatia,10370, miroslav.bukan@bc-institut.hr
Published in „GENETIKA“ Vol. 47, No. 1(2015), pp. 245-251
© 2015 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575
Original scientific paper
GENETIC VARIABILITY OF Verbascum POPULATIONS FROM METAL POLLUTED AND UNPOLLUTED SITES
Gordana DANILOVIĆ1, Filis MORINA2 Zlatko SATOVIC3, Ljiljana PROKIĆ4,
and Dejana PANKOVIĆ1
1Faculty of Environmental Protection, Educons University, Sremska Kamenica, Serbia
2Institute for Multidisciplinary Research, University of Belgrade, Belgrade, Serbia
3Faculty of Agriculture, University of Zagreb, Zagreb, Croatia
4Faculty of Agriculture, University of Belgrade, Belgrade, Serbia
Abstract
Danilović G., F. Morina, Z. Satovic, Lj. Prokić, and D. Panković (2015): Genetic variability of Verbascum populations from metal polluted and unpolluted sites.- Genetika, Vol 47, No. 1, 245-251.
Mullein (Verbascum) plants have extensive distribution and can grow in variable environmental conditions. Seed was collected from mullein plants grown at 4 locations, two metals contaminated and two metal uncontaminated areas. Genetic variability of progeny was examined. Populations collected from unpolluted areas were genetically more similar than those collected from polluted areas as revealed by RAPD and SSR markers and UPGMA analysis. The results indicate that there is genetic differentiation between examined populations and therefore they represent suitable material for further investigation of plant adaptation mechanisms to increased metal content.
Key words: metal tolerance, mullein,RAPD, SSR, UPGMA
Corresponding author: Dejana Panković, Faculty of Environmental Protection, Educons University, 21208 Sremska Kamenica, Serbia; Phone: +381214893667; Fax: +381214893618; E-mail: dejanapankovic@yahoo.co.uk; dejana.pankovic@educons.edu.rs
Published in „GENETIKA“ Vol. 47, No. 1(2015), pp. 253-263
© 2015 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575
Original scientific paper
A LINKAGE MAP OF CHICKPEA (Cicer arietinum L.) BASED ON POPULATION FROM ILC3279×ILC588 CROSSES: LOCATION OF GENES FOR TIME TO FLOWERING, SEED SIZE AND PLANT HEIGHT
Ezzat KARAMI1*, Reza TALEBI1, Mehrab KHARKESH2 and Abbas SAIDI2
1Department of Plant breeding, Islamic Azad University, Sanandaj Branch, Sanandaj, Iran
2Department of Biotechnology, College of New Technologies and Energy Engineering, Shahid Beheshti University, GC., Tehran, Iran
.
Abstract
Karami E., R. Talebi, M. Kharkesh, and A. Saidi ( (2015): A linkage map of chickpea (Cicer arietinum L.) based on population from ILC3279×ILC588 crosses: location of genes for time to flowering, seed size and plant height.- Genetika, Vol 47, No. 1, 253 -263.
Quantitative traits of seed size, plant height and days to flowering were studied in a chickpea intraspecific F3:4 lines population derived from a ILC3279×ILC588 cross. The lines were genotyped with random amplified polymorphic DNA (RAPD), universal rice primer (URP) and sequence tagged microsatellite site (STMS) markers, and a genetic map composed of 7 linkage groups (LGs) covering 285.3 cM was constructed. Quantitative trait loci (QTLs) for the three characters were detected in LG2, LG3 and LG4. Two QTLs for days to flowering were detected on LG2 and LG3. These two QTLs accounted for 58% of the total phenotypic variation for days to flowering. A QTL for plant height was located in LG3 explaining around 42% of the variation. This trait was shown to be under a major gene control. For 100-seed weight, a QTL located in LG4 explained around 37% of the phenotypic variations. This information can be used to formulate the an efficient breeding strategy for improvement of time to flowering in short-season temperate environments, plant height with more reproductive biomass and improved yield with bigger seed size in chickpea.
Key words: Chickpea, Mapping, QTLs, Yield related traits.
Corresponding author: Ezzat Karami, Department of Plant breeding, Islamic Azad University, Sanandaj Branch, Sanandaj, Iran, email:ezzatut81@yahoo.com
Published in „GENETIKA“ Vol. 47, No. 1(2015), pp. 265-274
© 2015 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575
Original scientific paper
GENETIC VARIABILITY AND TRAITS ASSOCIATION IN CASTOR BEAN
(Ricinus communis L.)
Farnaz Goodarzi1, Abbas Hassani1, Reza Darvishzadeh2,3 and Hamid Hatami Maleki*4
1Department of Horticulture, Faculty of agriculture, Urmia University, Urmia, Iran
2Department of Plant Breeding and Biotechnology, Faculty of agriculture, Urmia University, Urmia, Iran
3Institue of Biotechnology, Urmia University, Urmia, Iran
4Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Maragheh, Maragheh, Iran
Abstract
Goodarzi F., A. Hassani, R. Darvishzadeh and H. Hatami Maleki (2015): Genetic variability and traits association in castor bean (Ricinus communis L.)- Genetika, Vol 47, No. 1, 265 - 274.
Genetic diversity of 12 castor bean accessions collected from different geographical regions of Iran was assessed in a randomized complete block design with three replications under filed condition. The data were recorded for 32 agro-morphological traits. Significant differences were observed among accessions for main stem length, main stem moist weight, main stem dry weight, 10-seeds weight on primary raceme, seed number on primary raceme, leaf area dry weight, female flower length, male flower length, secondary and tertiary raceme weight and oil percentage. A strongly positive correlation was observed between total seed weight on primary raceme as yield with seed number on primary raceme, female flower length, primary raceme length and main stem diameter. Path coefficient analysis indicated high direct effect of seed number on primary raceme (0.82) on seed yield. In addition, direct effect of primary raceme length on seed yield was negative (-0.13). Primary raceme length had the greatest indirect effect via seed number on seed yield (0.35).
Key words: agro-morphological traits, castor bean, correlation coefficient, genetic diversity, path coefficient analysis
Corresponding author: Hamid Hatami Maleki, Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Maragheh, Maragheh, Iran, Tel.: +98 9148964510. E-mail address: hatamimaleki@maragheh.ac.ir
Published in „GENETIKA“ Vol. 47, No. 1(2015), pp 275-288
© 2015 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:630
Original scientific paper
DIVERSITY AND GENETIC STRUCTURE OF Ornithogalum L. (Hyacinthaceae) POPULATIONS AS REVEALED BY RAPD-PCR MARKERS
Andrijana ANDRIĆ, Nataša KOČIŠ TUBIĆ, Milica RAT, Dragana OBREHT VIDAKOVIĆ
Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad
Abstract
Andrić A., N. Kočiš Tubić, M. Rat, and Dragana Obreht Vidaković (2015): Diversity and genetic structure of Ornithogalum L. (hyacinthaceae) populations as revealed by RAPD-PCR markers.- Genetika, Vol 47, No. 1, 275-288.
Random amplified polymorphic DNA (RAPD) PCR method was used to assess the level of diversity and genetic structure in Ornithogalum L. populations from Serbia and Hungary with the main goal of improving the knowledge of this genus in the given region. The material was collected from 19 populations and identified as two morphologically similar and phylogenetically close taxa: O. umbellatum L. 1753 and O. divergens Boreau 1887. All ten RAPD primers used for the analysis gave PCR products, with length between 3000bp and 300bp. There were 101 amplified fragments in total; number of polymorphic bands per primer varied between seven and 13. Percentage of polymorphic loci was 96% in total and 12% in average in each population. Genetic variation statistics for all loci also showed that genetic diversity for all populations was 0.29 and Shannon index 0.45, while mean values for these parameters calculated for each population were 0.04 and 0.06, respectively. Analysis of molecular variance demonstrated high population genetic differentiation; however Mantel test showed no significant correlation between geographic distances of populations and genetic distances expressed through population pairwise FST. UPGMA dendrogram based on Jaccard genetic similarity coefficients showed subclustering and principal coordinate analysis based on Nei and Li coefficients of genetic distances indicated grouping. Analysis of populations genetic structure was in accordance with these results and clearly separated populations of O. umbellatum from O. divergens. RAPDs proved to be a reliable and rapid method suitable for distinguishing genetic differentiation in Ornithogalum, thus could be applied as a useful additional tool in resolving taxonomic problems.
Key words: Ornithogalum, RAPD, genetic diversity, genetic structure
Corresponding author: Andrijana Andrić, Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad, Trg Dositeja Obradovića 2, 21000 Novi Sad, Serbia; Telephone: +381641642350, Fax: 021 450 620, E-mail: andrijana.andric@dbe.uns.ac.rs
Published in „GENETIKA“ Vol. 47, No. 1(2015), pp. 289-294
© 2015 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-ZemunSerbia
UDC 575
Original scientific paper
STUDY ABOUT YIELD AND CUTTING’S LOSSES OF TWO TEA CLONE BUSHES
IN IRAN
Ahmad DADASHPOUR*1, Moazzam HASSANPOUR ASIL2, Reza HOJJAT-ANSARI2, Behrooz ALINAGHIPOUR3, and Yousef HAMIDOGHLI2
1Department of Horticulture, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
2Department of Horticulture, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
3Tea Research Institute, Lahijan, Iran
Abstract
Dadashpour A., M. Hassanpour Asil, R. Hojjat-Ansari, B. Alinaghipour, and Y. Hamidoghli (2015): Study about yield and cutting’s losses of two tea clone bushes in Iran.- Genetika, Vol 47, No. 1, 289-294.
In the first trial, study conducted to compare of yield between two shapes of pruning (curved and flat) of tea bushes (clone 100). After leaf harvesting, curved pruning bushes had more yield than flat pruning bushes. Thus curved pruning method can be advised to gardeners as a reliable and superior pruning method. In the secondary experiment, study was carried out to investigation of cutting’s losses in all head-cuttings and comparison between tow types bush in tea (100 and selective). Results showed that there is significant difference between 100 and selective clones at p≤0.05. It means that obtained mean cutting’s losses by selective was less than 100 bushes.
Key words: bush, curved and flat pruning, tea, vegetative propagation
Corresponding author: Ahmad Dadashpour, Department of Horticulture, University College of Agriculture & Natural Resources, University of Tehran, Karaj 31587-77871, Iran, email: dadashpour@can.ut.ac.ir
Published in „GENETIKA“ Vol. 47, No. 1(2015), pp. 295-301
© 2015 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:633.15
Original scientific paper
PATH ANALYSIS FOR MORPHOLOGICAL TRAITS IN MAIZE (Zea mays L.)
Jovan PAVLOV, Nenad DELIĆ, Ksenija MARKOVIĆ, Miloš CREVAR, Zoran ČAMDŽIJA, and Milan STEVANOVIĆ
Maize Research Institute Zemun Polje, Belgrade, Serbia
Abstract
Pavlov J., N. Delić, K. Marković, M. Crevar, Z. Čamdžija, and M. Stevanović (2015): Path analysis for morphological traits in maize (Zea mays L.)- Genetika, Vol 47, No. 1, 295-301.
Six inbred lines were crossed according to incomplete diallel method. In this way fifteen hybrid combinations were obtained. Hybrid combinations derived from these parental components were used in this work. The objective of study was to estimate direct and indirect effects of five morphological traits on grain yield by the application of the simple coefficient correlation and path coefficient analysis. The trait number of leaves above the top ear with the value of 0.736 has the strongest direct positive effect on grain yield. Positive direct effects on grain yield were also observed for height to tassel base and plant height, while negative direct effects were observed for ear height and total number of leaves. As far as indirect effects were considered, a positive effect of the ear position height on yield over the total plant height stands out. The evaluation of the proportion of mutual effects of five observed morphological traits on grain yield, expressed by the coefficient of multiple determination (R2y12345) amounted to 0.7881.
Key words: correlations, maize, path analysis
Corresponding author: Jovan Pavlov, Maize Research Institute Zemun Polje, Slobodana Bajića 1, 11185 Zemun Polje, Serbia, Tel.: + 381 64 84 06 198, Fax: + 381 11 3756 707e-mail: jpavlov@mrizp.rs
Published in „GENETIKA“ Vol. 47, No. 1(2015), pp. 303-310
© 2015 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575
Original scientific paper
FAMILY HISTORY BASED APPROACH IN RISK PREDICTION FOR PARKINSON'S DISEASE: ADDITIONAL CONTRIBUTION OF FAMILIAL ASSOCIATED DISORDERS
Irena VRECAR1, Aleš MAVER1 , Zvezdan PIRTOŠEK2, Dejan GEORGIEV2, Zalika KLEMENC KETIŠ3, and Borut PETERLIN1
1Clinical Institute of Medical Genetics, University Medical Centre Ljubljana, Ljubljana, Slovenia
2Department of Neurology, University Medical Centre Ljubljana, Ljubljana
3Department of Family Medicine, Medical School, University of Ljubljana, Ljubljana, Slovenia
Abstract
Vrecar I., A. Maver , Z. Pirtošek, D. Georgiev, Z. Klemenc Ketiš, and B. Peterlin (2015): Family history based approach in risk prediction for Parkinson's disease: additional contribution of familial associated disorders- Genetika, Vol 47, No. 1, 303- 310.
The aim of our study was to examine the contribution of family history of Parkinson's disease and its associated disorders in the assessment of predictive capacity of risk models for Parkinson’s disease. In a population of 192 patients with Parkinson’s disease and 1659 healthy individuals we investigated the impact of environmental factors and the effects of family history on Parkinson's disease risk. Pesticides exposure, positive family history of Parkinson’s disease and a positive family history of dementia and melanoma were associated to an increased risk for Parkinson’s disease, with results regarding family history of depression near to statistical significance. Smoking and caffeine intake were associated to a decreased risk for Parkinson’s disease. Three risk prediction models were assessed using the area under the curve approach: first model was based on known environmental risk factors, in the second model we added family history of Parkinson’s disease and in the third model we additionally included family history of dementia, melanoma and depression. We showed that inclusion of data on family history of associated disorders (AUC 0.76) improves predictive capacity of risk model for Parkinson’s disease in comparison with the first (AUC 0.62) and the second model (AUC 0.71). We concluded that family history of associated disorders: dementia, depression and melanoma improves predictive capacity of risk models for Parkinson’s disease.
Key words: Parkinson’s disease, family history, risk prediction
Corresponding author: Borut Peterlin, Clinical Institute of Medical Genetics.Department of Obstetrics and GynecologyUKC Ljubljana.Šlajmerjeva 3.SI-1000 Ljubljana.Slovenia.Tel/ fax +38615401137, E-mail: borut.peterlin@guest.arnes.si
Published in „GENETIKA“ Vol. 47, No. 1(2015), pp. 311-321
© 2015 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575
Original scientific paper
SCREENING OF B CHROMOSOMES FOR PRESENCE OF TWO GENES IN YELLOW-NECKED MICE, Apodemus flavicollis (Mammalia, Rodentia)
Marija RAJIČIĆ∗, Tanja ADNAĐEVIĆ, Gorana STAMENKOVIĆ, Jelena BLAGOJEVIĆ
and Mladen VUJOŠEVIĆ
Department of Genetic Research, Institute for Biological Research “Siniša Stanković”, University of Belgrade, Belgrade, Serbia
Abstract
Rajičić M., T. Adnađević, G. Stamenković, J. Blagojević and M. Vujošević (2015): Screening of b chromosomes for presence of two genes in yellow-necked mice, Apodemus flavicollis (Mammalia, Rodentia) - Genetika, Vol 47, No. 1, 311-321
B chromosomes (Bs) are a very heterogeneous group of extra chromosomes. In various species Bs occur with different nucleotide sequences ranging from repetitive to protein coding. In yellow-necked field mice, Apodemus flavicollis Bs are small euchromatic chromosomes and untill now, only few molecular analyses have been conducted. In this study we examined A. flavicollis individuals with different number of Bs for presence of two genes, C-KIT and 18S rRNA. The C-KIT proto-oncogene was found on Bs in three Canidae species and one Cervidae species. This gene is a coding receptor critical for proliferation and cell differentiation of hematopoietic, melanoblast and primordial germ cells, and is highly conserved within mammals. While using semiquantitative PCR, we did not notice any difference in the C-KIT band intensity among animals with different number of Bs (0-3). The presence of only one copy of C-KIT gene was confirmed using real time-PCR on genomic DNA of A. flavicollis specimens with different number of Bs. rRNA genes in eukaryotes’ genome are organized like units of tandem repeated sequences. The units form distinct clusters on one to several chromosome pairs. rRNA genes were found on Bs in different species including two species of genus Apodemus. One particular sample with 2 Bs showed the number of 18S rRNA gene about three times that of the calibrator 0 B sample. This result can indicate the presence of 18S rRNA gene on Bs, but its confirmation requires the implementation of other methods. Still, we can neither confirm nor deny the existence of pseudogen of tested target genes, or lose of exon 1 of C-KIT protooncogen in Bs of A. flavicollis. Our findings are further discussed.
Key words: B chromosomes, C-KIT, 18S rRNA, Apodemus flavicollis, semiquantitative PCR
Corresponding author: Marija Rajičić, Department of Genetic Research, Institute for Biological Research “Siniša Stanković”, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia Tel.: +381 11 2078333; fax: +381 11 2761433, E-mail address: marija.rajicic@ibiss.bg.ac.rs
Published in „GENETIKA“ Vol. 47, No.1(2015), pp. 323-337
© 2015 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575
Original scientific paper
MOLECULAR CHARACTERIZATION AND EVOLUTION STUDIES OF A SERK LIKE GENE TRANSCRIPTIONALLY INDUCED DURING SOMATIC EMBRYOGENESIS IN Phoenix Dactylifera L v Deglet Nour
Imen REKIK1, Noureddine DRIRA1, C. Douglas GRUBB2, and Amine ELLEUCH1
1Laboratory of plant biotechnology, Faculty of sciences of Sfax, University of Sfax. Tunisia
2Leibniz Institute of Plant Biochemistry, Halle, Germany
Abstract
Rekik I., N. Drira, C. D. Grubb, and A. Elleuch (2015): Molecular characterization and evolution studies of a serk like gene transcriptionally induced during somatic embryogenesis in Phoenix Dactylifera L v Deglet Nour.- Genetika, Vol 47, No. 1, 323-337
A somatic embryogenesis receptor kinase like (SERKL) cDNA, designated PhSERKL, was isolated from date palm (Phoenix Dactylifera L) using RACE PCR. PhSERKL protein shared all the characteristic domains of the SERK family, including five leucine-rich repeats, one proline-rich region motif, a transmembrane domain, and kinase domains. Phylogenetic analyses using PHYLIP and Notung 2.7 programs suggest that the SERK proteins of some plant species resulted from relatively ancient duplication events. We predict an ancestor protein of monocots and dicots SERK using FASTML program. Somatic embryogenic cultures of date palm were established following transfer of callus cultures to medium containing 2, 4-dichlorophenoxyacetic acid. The role of PhSERKL gene during establishment of somatic embryogenesis in culture was investigated using quantitative real-time PCR. PhSERKL gene was highly expressed during embryogenic competence acquisition and globular embryo formation in culture. Overall, levels of expression of PhSERKL gene were lower in nonembryogenic tissues and organs than in embryogenic callus.
Key words: duplication, expression pattern, Phoenix dactylifera, SERKL
Corresponding author: Imen Rekik,, Laboratory of plant biotechnology, Faculty of sciences of Sfax, University of Sfax. Tunisia,Tel: 0021674676616, Fax: 0021674274437, Email : imenbmc@yahoo.fr
Published in „GENETIKA“ Vol. 47, No.1(2015), pp. 339-348
© 2015 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575
Original scientific paper
EVALUATION OF DNA DAMAGE IN RAT LYMPHOCYTES EXPOSED TO TULATHROMYCIN IN VITRO
Jelena MALETIĆ1, Ninoslav DJELIĆ1, Milena RADAKOVIĆ1, Milan MALETIĆ1,
Nada LAKIĆ2, Vladimir KUKOLJ1, Nevenka ALEKSIĆ1, Marko ANDJELKOVIĆ3
and Zoran STANIMIROVIĆ1
1Faculty of Veterinary Medicine, University of Belgrade, Belgrade, Serbia
2Faculty of Agriculture, University of Belgrade, Belgrade, Serbia
3Serbian Academy of Science and Arts, Belgrade, Serbia
Abstract
Maletić J., N. Djelić, M. Radaković, M. Maletić, N. Lakić, V. Kukolj, N. Aleksić, M. Andjelković and Z. Stanimirović (2015): Evaluation of DNA damage in rat lymphocytes exposed to tulathromycin in vitro.- Genetika, Vol 47, No. 1, 339-348.
Tulathromycin is a relatively new semi-synthetic macrolide antibiotic, a member of the triamilide group, approved primarly for the treatment of respiratory diseases in cattle and swine. Various genotoxicological studies indicated that tulathromycin is not genotoxic, but no available published data originate from the single-cell gel electrophoresis (Comet) assay. Therefore, the objective of this study was to examine whether it can induce primary DNA damage using in vitro Comet assay in isolated rat lymphocytes. Lymphocytes were treated with a broad spectrum of tulathromycin concentrations (from 1 to 100 µM) and co-treatment with an antioxidant, catalase (100 IU/mL and 500 IU/mL) was performed. The highest concentrations of tulathromycin (50 and 100 µM) caused significant increase of DNA damage in rat lymphocytes and catalase did not significantly reduce the DNA-damaging effect of tulathromycin. The results of this study indicate that tulathromycin induces genotoxic effects at high concentrations, that catalase does not exert protective effect in this case.
Key words: comet assay, DNA damage, rat lymphocytes, tulathromycin
Corresponding author: Ninoslav Djelić, Department of Biology,Faculty of Veterinary Medicine University of Belgrade,Oslobodjenja Blvd. 18,11000 Belgrade, Serbia, Phone: +381 11 26 58 894; Cell phone: +381 64 155 83 14; Fax: +381 11 26 85 936; E-mail: ndjelic@vet.bg.ac.rs
Published in „GENETIKA“ Vol. 47, No.1(2015), pp. 349-356
© 2015 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575
Original scientific paper
INHERITANCE OF GYNOECISM IN CUCUMBER (Cucumis sativus L.) USING GENOTYPE GBS-1 AS GYNOECIOUS PARENT
Kalidas PATI1, Anilabh Das MUNSHI2 and Tusar Kanti BEHERA2
1ICAR- Central Tuber Crops Research Institute, Regional centre, Bhubaneswar, Odisha, India
2ICAR- Indian Agricultural Research Institute, New Delhi, India
Abstract
Pati K., A. Das Munshi and T. Kanti Behera (2015): Inheritance of gynoecism in cucumber (Cucumis sativus L.) using genotype gbs-1 as gynoecious parent- Genetika, Vol 47, No. 1, 349-356.
The inheritance pattern of gynoecious sex expression in cucumber was studied by utilizing a gynoecious line (GBS-1) and two monoecious lines (Pusa Uday and Punjab Naveen). Crosses were made between gynoecious line (GBS-1) and monoecious lines (Pusa Uday and Punjab Naveen). The F1 and F2 population along with parental lines were evaluated to study the inheritance of this trait. All F1 hybrids showed gynoecious sex in both crosses and in the F2 generation, the observed distribution of plant phenotypes fitted the expected mendelian ratio of 3 (gynocious plant) : 1 (monoecious plant). The segregation of plant sex types suggested monogenic dominant control of gynoecious sex form in cucumber using genotype GBS-1 .
Key words: Cucumber, Gynoecious sex, Inheritance
Corresponding author: Kalidas Pati, ICAR- Central Tuber Crops Research Institute, Regional centre, Bhubaneswar, Odisha, 751019, India. Phone: +917377914855, e-mail:kalidas9555@gmail.com
Published in „GENETIKA“ Vol. 47, No.1(2015), pp. 357-373
© 2015 Serbian Genetics
Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575
Review paper
70 YEARS OF RESEARCH ON THE AMERICAN MINK (Neovison vison SCHREB., 1777) GENETICS – WHERE ARE WE NOW?
Jakub Jan SKORUPSKI
Department of Genetics and Animal Breeding, West Pomeranian University of Technology
in Szczecin, Poland
Abstract
Skorupski J. J. (2015): 70 years of research on the american mink (Neovison vison schreb., 1777) genetics – where are we now?- Genetika, Vol 47, No. 1, 357-373
The purpose of this review is to present the current state of knowledge about the genetics of the American mink (Neovison vison Schreb., 1777) – a species that achieved in the twentieth century an unprecedented ecological success associated with the dynamic development of its economic use. However, despite the large popularity and economic importance of the American mink as a fur animal, and the scale of the problems associated with its introduction beyond the range of natural occurrence, genetic research, particularly molecular genetics and genomics of this species, show relatively little progress. The article contains a comprehensive description of the studies undertaken on the genetics of the species, both in terms of cytogenetics, molecular genetics, genomics, population genetics and phylogenetics. The progress of the genome sequencing project of the American mink is also described as well as its transcriptome annotation. The article also deals with still unexplained and not completed, despite 70 years of genetic research, issues such as the standard karyotype, the precise molecular basis of coat color inheritance and systematic position of the species.
Key words: American mink, genetics, cytogenetics, molecular genetics, genomics, phylogenetics, genome sequencing, transcriptomics
Corresponding author: Jakub Jan Skorupski, Department of Genetics and Animal Breeding, West Pomeranian University of Technology in Szczecin, Piastów 45 Ave., 70-311 Szczecin, Poland; phone: +48 512 014 658; e-mail: jakub@gajanet.pl