GENETIKA, Vol

GENETIKA, Vol. 46, No. 3 (2014)

Ivana STOŠIĆ, Darko GRUJIČIĆ, Slobodan ARSENIJEVIĆ, Olivera MILOŠEVIĆ-DJORDJEVIĆ

INFLUENCE OF GLUTATHIONE – S – TRANSFERASE (GSTT1 AND GSTM1) POLYMORPHISM ON BASELINE MICRONUCLEI FREQUENCY IN PERIPHERAL BLOOD LYMPHOCYTES [Abstract] [Full text]

Emre YÖRÜK, Aylin GAZDAĞLI, Gülruh ALBAYRAK

CLASS B TRICHOTHECENE CHEMOTYPING IN Fusarium SPECIES BY PCR ASSAY [Abstract] [Full text]

Milica FOTIRIĆ AKŠIĆ, Vera RAKONJAC, Dragan NIKOLIĆ,Slavica ČOLIĆ, Dragan MILATOVIĆ, Vlado LIČINA, Dragan RAHOVIĆ

EFFECTIVE POLLINATION PERIOD IN ‘OBLAČINSKA’ SOUR CHERRY CLONES [Abstract] [Full text]

Sanjay KUMAR

STUDIES ON EFFICIENCY OF RAPD PRIMERS IN DEVELOPING MOLECULAR PROFILES FOR GENETIC PURITY STUDIES IN SOYBEAN (Glycine max L.) CULTIVARS [Abstract] [Full text]

Bojan kenig, Aleksandra patenković, Marko anđelković, Marina stamenković-radak

LIFE-HISTORY VARIATION OF Drosophila subobscura UNDER LEAD POLLUTION DEPENDS ON POPULATION HISTORY [Abstract] [Full text]

Yuksel KAYA, Emel OZER

PARAMETRIC STABILITY ANALYSES OF MULTI-ENVIRONMENT YIELD TRIALS IN TRITICALE (xTriticosecale Wittmack) [Abstract] [Full text]

Vera RAKONJAC, N. KORAĆ, S. TODIĆ, M. MEDIĆ, Z. BEŠLIĆ, I. KULJANČIĆ, D. IVANIŠEVIĆ and M. POPOV

GENETIC DIVERSITY OF A SERBIAN GRAPEVINE GERMPLASM COLLECTION BASED ON MORPHOAGRONOMIC CHARACTERISTICS [Abstract] [Full text]

Zahra Aghaali, Morteza Ghadmizadeh, Babak Abdollahi Mandoulakani, Iraj Bernousi

IRAP and REMAP-based assessment of genetic diversity in chickpea collection from Iran [Abstract] [Full text]

Jelena M. ALEKSIĆ, Jovanka MILJUŠ-ĐUKIĆ, Živko JOVANOVIĆ, Branko TOMIĆ and Bojana BANOVIĆ

Molecular tools for utilization of mitochondrial diversity in faba bean (Vicia faba) [Abstract] [Full text]

Mostafa KHODADADI, Hamid DEHGHANI, Mohammad Hossein FOTOKIAN

GENETIC DIVERSITY OF WHEAT GRAIN QUALITY AND DETERMINATION THE BEST CLUSTERING TECHNIQUE AND DATA TYPE FOR DIVERSITY ASSESSMENT [Abstract] [Full text]

VioletaANDJELKOVIC, Natalija KRAVIC, Vojka BABIC, Dragana IGNJATOVIC-MICIC, Zoran DUMANOVIC, Jelena VANCETOVIC

ESTIMATION OF DROUGHT TOLERANCE AMONG MAIZE LANDRACES FROM MINI-CORE COLLECTION [Abstract] [Full text]

Baoguang JIA, Qing LIN, Lin ZHANG, Xiaofeng TAN, Xiaolin LEI, Xiaoyi HU, Fenggong SHAO²DEVELOPMENT OF 15 GENIC-SSR MARKERS IN OIL-TEA TREE (Camellia oleifera) BASED ON TRANSCRIPTOME SEQUENCING [Abstract] [Full text]

Borislav BANJAC, Velimir MLADENOV, Miodrag DIMITRIJEVIĆ, Sofija PETROVIĆ, Jan BOĆANSKI

GENOTYPE × ENVIRONMENT INTERACTIONS AND PHENOTYPIC STABILITY FOR WHEAT GROWN IN STRESSFUL CONDITIONS [Abstract] [Full text]

Boris KRAPTCHEV, Roumiana VASSILEVSKA-IVANOVA, Lydia SHTEREVA

HETEROSIS AND ANTIOXIDANT COMPOUNDS OF SWEET CORN BREEDING LINES AND THEIR F₁ HYBRID [Abstract] [Full text]

Mahdi BAYAT, Reza DARVISHZADEH, Farough SOLEIMANI, Seyyed Reza ALAVI

SEQUENTIAL PATH ANALYSIS FOR DETERMINING INTERRELATIONSHIPS BETWEEN YIELD AND RELATED TRAITS IN TOBACCO (Nicotiana tabacum L.) UNDER NORMAL AND ABIOTIC STRESS CONDITIONS [Abstract] [Full text]

Nitiprasad Namdeorao JAMBHULKAR and Lotan Kumar BOSE

GENETIC VARIABILITY AND ASSOCIATION OF YIELD ATTRIBUTING TRAITS WITH GRAIN YIELD IN UPLAND RICE [Abstract] [Full text]

Vesna PERIĆ, Ana NIKOLIĆ, Vojka BABIĆ, Aleksanda SUDARIĆ, Mirjana SREBRIĆ, Vuk ĐORĐEVIĆ, Snežana MLADENOVIĆ DRINIĆ

Genetic relatedness of soybean genotypes based on agromorphological traits and rapd markers [Abstract] [Full text]

Jana ŽIAROVSKÁ, Milan BEŽO, Michaela HRDLIČKOVÁ, Eloy FERNANDÉZ

STANDARDIZATION AND REPRODUCIBILITY OF RANDOM MARKER BASED ANALYSIS OF MICROPROPAGATED CRIMSON BEEBALM [Abstract] [Full text]

Martina MILUCHOVA, Michal GABOR, Anna TRAKOVICKA

Analysis of genetic structure in Slovak Pinzgau cattle using five candidate genes related to milk production traits [Abstract] [Full text]

Xiaobo FAN, Alongkoad TANOMTONG, Arunrat CHAVEERACH, Krit PINTHONG,^, Siripiyasing PORNNARONG, Weerayuth SUPIWONG, Thomas LIEHR, Anja WEISE

HIGH RESOLUTION KARYOTYPE OF THAI CRAB-EATING MACAQUE (Macaca fascicularis) [Abstract] [Full text]

Marija Mrdaković, Biljana Stojković, Larisa Ilijin, Milena Vlahović, Vesna Perić-Mataruga, Jelica Lazarević

Testing the adaptive plasticity of gypsy moth digestive enzymes in response to tannic acid using phenotypic selection analysis GERMPLASM [Abstract] [Full text]

Violeta oro, Bogdan nikolić and Dragana jošić

THE “POTATO ROAD” AND BIOGEOGRAPHIC HISTORY OF POTATO CYST NEMATODE POPULATIONS FROM DIFFERENT CONTINENTS [Abstract] [Full text]

Peyman SHARIFI

CORRELATION AND PATH COEFFICIENT ANALYSIS OF YIELD AND YIELD COMPONENT IN SOME OF BROAD BEAN (Vicia faba L.) GENOTYPES [Abstract] [Full text]

Jiri SOCHOR, Tunde JURIKOVA, Sezai ERCISLI, Jiri MLCEK, Mojmir BARON, Stefan BALLA, Suzan Ozturk YILMAZand Thomas NECAS

CHARACTERIZATION OF CORNELIAN CHERRY (Cornus mas L.) GENOTYPES - GENETIC RESOURCES FOR FOOD PRODUCTION IN CZECH REPUBLIC [Abstract] [Full text]

Abdulmojeed YAKUBU, Daniels Ewaoche ALADE and Ndubuisi I. DIM

MOLECULAR ANALYSIS OF SOLUTE CARRIER FAMILY 11 MEMBER A1 (SLC11A1) GENE IN RUMINANTS AND NON-RUMINANTS USING COMPUTATIONAL METHOD [Abstract] [Full text]

Radosav CEROVIĆ, Zorica PAJIĆ, Milomir FILIPOVIĆ,Milica FOTIRIĆ-AKŠIĆ, Sanja RADIČEVIĆ, Dragan NIKOLIĆ, Milena ĐORĐEVIĆ

POLLEN GERMINATION AND POLLEN TUBE GROWTH IN ZP MAIZE LINES [Abstract] [Full text]

Ramadan Salem AHSYEE, SanjaVASILJEVIĆ, Irena ĆALIĆ, Miroslav ZORIĆ, Đura KARAGIĆ, Gordana ŠURLAN-MOMIROVIĆ

GENETIC DIVERSITY IN RED CLOVER (Trifolium pratense L.) USING SSR MARKERS [Abstract] [Full text]

Zoran MAKSIMOVIĆ, Dijana ČORTAN, Vladan IVETIĆ, Snežana MLADENOVIĆ DRINIĆ, Mirjana ŠIJAČIĆ-NIKOLIĆ

GENETIC STRUCTURE OF BLACK POPLAR (Populus nigra L.) POPULATION IN THE AREA OF GREAT WAR ISLAND [Abstract] [Full text]

Ali Reza GHASEMI, Ahmad Reza GOLPARVAR, Mehdi Nasr ISFAHANI

ANALYSIS OF GENETIC DIVERSITY OF SUGAR BEET GENOTYPES USING RANDOM AMPLIFIED POLYMORPHIC DNA MARKER [Abstract] [Full text]

M.K.SINGH, N.B.SINGH, S.THAKUR, P.K.NAIK

MOLECULAR EVALUATIONS OF THIRTY ONE CLONES OF POPLAR BASED ON RAPD AND SSR MOLECULAR MARKERS [Abstract] [Full text]

Slavica POPOVIĆ BUBUJUK, Adrian MUSTER; Ninoslav DJELIĆ; Dragan KATARANOVSKI, Marko ANDJELKOVIĆ

INDUCTION OF MICRONUCLEI BY CADMIUM CHLORIDE IN AO RATS DEPENDS ON AGE AND SEX [Abstract] [Full text]

M.VIMALADEVI, .B.KALAAVATHI

A MICROARRAY GENE EXPRESSION DATA CLASSIFICATION USING HYBRID BACK PROPAGATION NEURAL NETWORK [Abstract] [Full text] [Correction]

Aleksandra GOVEDARICA-LUČIĆ, Mirjana MOJEVIĆ, Goran PERKOVIĆ, Branka GOVEDARICA

YIELD AND NUTRITIONAL QUALITY OF GREENHOUSE LETTUCE (Lactuca sativa L.) AS AFFECTED BY GENOTYPE AND PRODUCTION METHODS [Abstract] [Full text]

Muhammad IRFAN, Jia-xing SUN, Yanbin LIU, Xue LI, Shuang YANG

GENETIC ANALYSIS OF CHLOROPHYLL CONTENT IN MAIZE BY MIXED MAJOR AND POLYGENE MODELS [Abstract] [Full text]

Ankica KONDIĆ-ŠPIKA, Milica NIČIĆ, Ljiljana BRBAKLIĆ, Dragana TRKULJA, Dragana MILADINOVIĆ, Siniša JOCIĆ, Milka BRDAR, Nikola HRISTOV

MICROSATELLITES IN THE ANALYSIS OF WHEAT GENETIC DIVERSITY [Abstract] [Full text]

Published in „GENETIKA“ Vol. 46, No. 3 (2014), pp. 649-659

UDC 575
DOI: 10.2298/GENSR1403649S

Original scientific paper

INFLUENCE OF GLUTATHIONE – S – TRANSFERASE (GSTT1 AND GSTM1) POLYMORPHISM ON BASELINE MICRONUCLEI FREQUENCY IN PERIPHERAL BLOOD LYMPHOCYTES

Ivana STOŠIĆ¹, Darko GRUJIČIĆ¹, Slobodan ARSENIJEVIĆ²,

Olivera MILOŠEVIĆ-DJORDJEVIĆ^1,2

¹Faculty of Science, University of Kragujevac, Kragujevac, Serbia

²Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia

Abstract

Stošić I., D. Grujičić, S. Arsenijević, and O. Milošević-Djordjević (2014): Influence of glutathione – s – transferase (gstt1 and gstm1) polymorphism on baseline micronuclei frequency in peripheral blood lymphocytes. - Genetika, Vol 46, No. 3,649 -659.

We have analyzed impact of polymorphism in GSTT1 and GSTM1 genes on the micronuclei (MN) frequency in peripheral blood lymphocytes (PBLs). A total 134 women from central Serbia were enrolled in the study. Polymorphisms of GST genes were genotyped by performing a multiplex polymerase chain reaction (PCR) and cytokinesis block micronucleus (CBMN) test was used to assess MN frequency. GSTT1 null and GSTM1 null genotype carriers had higher MN frequencies as compared to positive counterparts but without statistical significance. Carriers of dual GSTT1/GSTM1 null genotypes had significantly higher MN frequency than positive/positive, positive/null and null/positive. Smokers and women >45 years old with GSTT1 null genotype and GSTT1null/GSTM1null genotypes have statistically higher MN frequency than positive counterparts. Results suggest possible influence of dual null genotypes of GSTT1/GSTM1 on the baseline MN frequency, as well influence on the level of MN in smokers and in women age >45 years. GSTT1 null genotype may have the potential to influence the baseline MN frequency in PBLs of smokers, as well as in women age >45 years.

Key words: GSTT1, GSTM1, micronuclei, peripheral blood lymphocytes, polymorphism

Corresponding author: Ivana Stošić, Faculty of Science, University of Kragujevac, Radoja Domanovića 12, P.O. Box 60, 34 000 Kragujevac, Serbia, e-mail: stosicster@gmail.com

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Published in „GENETIKA“ Vol. 46, No. 3 (2014), pp. 661 -669

Serbia

UDC 575
DOI:10.2298/GENSR1403661Y

Original scientific paper

CLASS B TRICHOTHECENE CHEMOTYPING IN Fusarium SPECIES

BY PCR ASSAY

Emre YÖRÜK¹, Aylin GAZDAĞLI¹, Gülruh ALBAYRAK²

¹Programme of Molecular Biology and Genetics, Institute of Science, Istanbul University Istanbul, Turkey

²Department of Molecular Biology and Genetics, Faculty of Science, Istanbul University, Istanbul, Turkey

Abstract

Yörük E., A. Gazdağli, and G. Albayrak (2014): Class B trichothecene chemotyping in Fusarium species by PCR assay. - Genetika, Vol 46, No. 3,661- 669.

Fusarium isolates are divided into three chemotypes according to produce of class B trichothecenes; 3-acetyldeoxynivalenol (3ADON) 15-acetyldeoxynivalenol (15ADON) and nivalenol (NIV) and 4- acetyldeoxynivalenol (NIV) chemotypes. In this study, chemotyping of seventeen isolates from Turkey and Iran belonging to F. graminearum, F. culmorum, F. poae and F. pseudograminearum species were carried out by polymerase chain reaction (PCR) analysis. While all F. culmorum and F. poae isolates determined as 3ADON, remaining F. graminearum and F. pseudograminearum isolates were either 3ADON or 15ADON chemotypes. A common band of 583 bp long DNA fragment was amplified in all of F. culmorum and F. poae, one F. pseudograminearum (21F) and four F. graminearum (14F, sh14, sh15, sh7) isolates with 3ADON chemotype. However, remaining two F. pseudograminearum and four F. graminearum isolates with 15ADON chemotype, yielded amplicons that of 863 bp. It was shown that 3ADON was more predominant chemotype from other class B trichothecenes. This is the first report on chemotyping of F. poae and F. pseudograminearum isolates and also to show presence of 3ADON chemotype in F. graminearum isolate from Turkey.

Key words: Class B trichothecenes, Fusarium graminearum, F. culmorum, F. poae, F. pseudograminearum;

Corresponding author: Gülruh Albayrak, Department of Molecular Biology and Genetics, Faculty of Science, Istanbul University, 34134 Vezneciler, Istanbul, Turkey, Tel: +90 (0)212 4555700,Fax: +90 (0) 212 5190834,e-mail: gulruh@istanbul.edu.tr

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Published in „GENETIKA“ Vol. 46, No. 3 (2014), pp. 671- 680

Serbia

UDC 575
DOI: 10.2298/GENSR1403671A

Original scientific paper

EFFECTIVE POLLINATION PERIOD IN ‘OBLAČINSKA’ SOUR CHERRY CLONES

Milica FOTIRIĆ AKŠIĆ¹*, Vera RAKONJAC¹, Dragan NIKOLIĆ¹,Slavica ČOLIĆ², Dragan MILATOVIĆ¹, Vlado LIČINA¹, Dragan RAHOVIĆ²

¹University of Belgrade, Faculty of Agriculture, Belgrade, Serbia

²Institute for Science Application in Agriculture, Belgrade, Serbia

Abstract

Fotirić Akšić M., V. Rakonjac, D. Nikolić,S. Čolić, D. Milatović, V. Ličina, D. Rahović (2014): Effective pollination period in ‘Oblačinska’ sour cherry clones. - Genetika, Vol 46, No. 3,671- 680.

To obtain high yields there should be high flower density and fruit set in sour cherry (Prunus cerasus L.) production. Furthermore, in order to ensure successful fertilization, there should be satisfactory stigma receptivity, rapid pollen tube growth along the style, as well as adequate ovule longevity. This manuscript presents the study of the effective pollination period (EPP) of four ‘Oblačinska’ sour cherry clones (II/2, III/9, XI/3 and XIII/1) that differs in pollen germination, fruit set and yields. In order to estimate EPP, pollination was conducted in six different stages of flower development: balloon stage, 2 d before anthesis (-2), at anthesis (0), and 2, 4, 6 and 8 d after anthеsis (DAA). The initial (IFS) and final fruit set (FFS) were recorded under the field conditions. Alongside with this, the rate of pollen tubes growth in the style was observed with fluorescent microscopy. The experimental design was completely randomized, a two-factorial analysis of variance was carried out and individual testing was performed using LSD test (p ≤ 0.05; p ≤ 0.01). The experiment was set in triplicates. Regarding FFS, clones II/2 and III/9 showed the best results (p ≤ 0.01) in 4 and 6 DAA. The number of pollen tubes in the style of the pistil decreased with subsequent terms of pollination, while its number in the ovule increased up to sixth day after pollination, followed by a decline. Clones II/2 and III/9 showed EPP which lasted from 6 to 8 d, while EPP found in clone XI/3, lasted only 2 d. It is concluded that only clone having long EPP should be used as parents for creating new sour cherry cultivars.

Key words: Class B trichothecenes, Fusarium graminearum, F. culmorum, F. poae, F. pseudograminearum;

Corresponding author: Milica Fotirić Akšić, Faculty of Agriculture, Nemanjina 6, 11080 Belgrade-Zemun, Serbia, phone: ++ 381 64 2612710, fax: ++ 381 11 2199 805, e-mail: fotiric@agrif.bg.ac.rs

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Published in „GENETIKA“ Vol. 46, No.3 (2014), pp. 681 -692

Serbia

UDC 575:633
DOI: 10.2298/GENSR1403681K

Original scientific paper

STUDIES ON EFFICIENCY OF RAPD PRIMERS IN DEVELOPING MOLECULAR PROFILES FOR GENETIC PURITY STUDIES IN SOYBEAN (Glycine max L.) CULTIVARS

Sanjay KUMAR

Genetics Laboratory, Department of Zoology, Banaras Hindu University, Varanasi, India

Abstract

Kumar S. (2014): Studies on efficiency of RAPD primers in developing molecular profiles for genetic purity studies in soybean (Glycine max L.) cultivars. - Genetika, Vol 46, No. 3, 681- 692.

Major advances have recently been made in our understanding of soybean genetics and of the application of new technologies to soybean improvement. Estimates of genetic relationships on the basisof the enzymes and molecular markers have been shown to be consistentwith expectations based on origin and pedigree information. To identify efficient markers, that are to be used for genetic purity studies, polymorphism is the basic criterion. RAPD has been found to be an effective and efficient tool to evaluate and reveal genetic polymorphism in several crop species. In present study a total of 80 RAPD primers were screened, out of which 37 gave amplification and only 30 primers showed unambiguous DNA profile. Out of these 30 primers, 22 gave polymorphic banding patterns. It is evident from the result that, 30/80 primers tried (38%) provided unambiguous amplification and out of 30 primers, 22 primers (73%) were found to be polymorphic. Scorable 30 RAPD primers led to amplification of 120 fragments out of which 81 (67.5%) bands were found to be polymorphic. On an average, we got 4 bands per primer and 15 primers (50%) have been found to produce more number of bands than the average value which is encouraging. 8 primers were found to give 100% polymorphisms. Our results are indicative of the efficiency of RAPD primers towards development of molecular profiles.

Key words: Glycine max, Polymorphism, PCR, RAPD, UPGMA

Corresponding author: Sanjay Kumar, Genetics Laboratory, Department of Zoology, Banaras Hindu University, Varanasi 225001, India (Phone: +91 7860448788; E-mail: sanjay_mbt@rediffmail.com)

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Published in „GENETIKA“ Vol. 46, No.3 (2014), pp. 693 -703

Serbia

UDC 575
DOI: 10.2298/GENSR1403693K

Original scientific paper

LIFE-HISTORY VARIATION OF Drosophila subobscura UNDER LEAD POLLUTION DEPENDS ON POPULATION HISTORY

Bojan kenig ¹, Aleksandra patenković¹, Marko anđelković ^1,2,3, Marina stamenković-radak^{1, 2}

¹Institute for Biological Research “Siniša Stanković”, Department of Genetics of Populations and Ecogenotoxicology, University of Belgrade, Belgrade, Serbia

²Faculty of Biology, University of Belgrade, Belgrade, Serbia

³Serbian Academy of Sciences and Arts, Belgrade, Serbia

Abstract

Kenig B., A. Patenković, M. Anđelković, M. Stamenković-Radak (2014): Life-history variation of Drosophila subobscura under lead pollution depends on population history - Genetika, Vol 46, No. 3, 693- 703.

Contamination represents environmental stress that can affect genetic variability of populations, thus influencing the evolutionary processes. In this study, we evaluate the relationship between heavy metal contamination (Pb) and phenotypic variation, assessed by coefficients of variation (CV) of life-history traits. To investigate the consequences of population origin on variation of life history traits in Drosophila subobscura in response to different laboratory conditions we compared populations from relatively polluted and unpolluted environments. Prior to experiment, flies from natural populations were reared for two generations in standard Drosophila laboratory conditions. Afterwards, all flies were cultured on three different media: one medium without lead as the control, and the other two with different concentrations of lead. Coefficients of variation (CV) of life-history traits (fecundity, egg-to-adult viability and developmental time) were analyzed on flies sampled in generations F2, F5and F8 from these three groups. In later generations samples from both polluted and unpolluted environments showed the increased fecundity variation on media with lead. This increase is expressed more in population from unpolluted environment. On contrary, population from unpolluted environment had increased variation of developmental time in earlier, F2 generation, compared to the population from polluted environment. Our results showed that the response to heavy metal contamination depends on the evolutionary history of the populations regarding habitat pollution.

Key words: Coefficient of variation (CV), Drosophila subobscura, Lead pollution, Life-history traits

Corresponding author: Bojan Kenig, Institute for Biological Research “Siniša Stanković”, Department of Genetics of Populations and Ecogenotoxicology, University of Belgrade, Bul. despota Stefana 142, 11060 Belgrade, Serbia,Phone: +381 11 20 78 328; Fax: +381 11 27 61 433; E-mail: bojan.kenig@ibiss.bg.ac.rs

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Published in „GENETIKA“ Vol. 46, No. 3 (2014), pp. 705 -718

Serbia

UDC 575:633
DOI: 10.2298/GENSR1403705K

Original scientific paper

PARAMETRIC STABILITY ANALYSES OF MULTI-ENVIRONMENT YIELD TRIALS

IN TRITICALE (xTriticosecale Wittmack)

Yuksel KAYA, Emel OZER

Bahri Dagdas International Agricultural Research Institute, Konya, Turkey

Abstract

Kaya Y. and E. Ozer (2014): Parametric stability analyses of multi-environment yield trials in triticale (xTriticosecale Wittmack) - Genetika, Vol 46, No. 3, 705- 718.

One of the main goals of Triticale (xTriticosecale Wittmack) Breeding Program of Turkey is to improve high yielding and stable genotypes across environments. In this study, 16 parametric stability methods were used to evaluate the genotype x environment interaction (GEI) in 9 (4 officially registered varieties and 5 advanced lines) triticale (xTriticosecale Wittmack) genotypes. The genotypes were evaluated for grain yield at 4 different locations for 3 years in rain-fed areas of Turkey. The testing locations have different climatic and edaphic conditions providing the conditions necessary for the assessment of stability. A combined analysis of variance, parametric stability statistics and rank correlations among them were determined. Significant differences were detected between genotypes and their GEIs. Different parametric stability statistics were used to determine stability of the studied genotypes. The level of association among the statistics was assessed using Spearman’s rank correlation. Rank-correlation coefficients between yield and some parametric stability statistics were highly significant. Genotypes mean yield was significantly correlated to the parametric stability statistics P_i (r = 0.95**), PCA1 (r = 0.87**) and D_i (r = 0.98**). A principal component analysis based on rank correlation matrix was performed for grouping the different parametric stability statistics studied. In conclusion, based on most parametric stability statistics, the genotype G8 was found to be the most stable and high yielding. This genotype is, therefore, recommended for release as a cultivar for rain-fed areas of Turkey.

Key words: genotype x environment interaction, yield, stability, Triticale (xTriticosecale Wittmack)

Corresponding author: Yuksel Kaya, Bahri Dagdas International Agricultural Research Institute, Konya, Turkey. Phone: +90 332 355 12 90 Fax: +90 332 355 12 88,e-mail: yuksel_k@yahoo.com

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Published in „GENETIKA“ Vol. 46, No. 3 (2014), pp.719 -730

Serbia

UDC 575.630
DOI: 10.2298/GENSR1403719R

Original scientific paper

GENETIC DIVERSITY OF A SERBIAN GRAPEVINE GERMPLASM COLLECTION BASED ON MORPHOAGRONOMIC CHARACTERISTICS

Vera RAKONJAC¹, N. KORAĆ², S. TODIĆ¹, M. MEDIĆ², Z. BEŠLIĆ^1*, I. KULJANČIĆ²,

D. IVANIŠEVIĆ² and M. POPOV²

¹ University of Belgrade, Faculty of Agriculture, Belgrade, Serbia

² University of Novi Sad, Faculty of Agriculture

Abstract

Rakonjac V., N.Korac, S.Todic, M.Medic, Z.Beslic, I.Kuljanic, D.Ivanisevic and M.Popov (2014): Genetic diversity of a Serbian grapevine germplasm collection based on morphoagronomic characteristics. - Genetika, Vol 46, No. 3, 719- 730.

The objective of this study was to evaluate diversity and relationships among grapevine cultivars from the Serbian gene bank in Sremski Karlovci, and to identify the most useful variables for discrimination. A total of 54 grapevine cultivars have been studied. During the period 2000–2005, twelve quantitative and qualitative characteristics were evaluated using OIV categories to each trait. Characterization was done using multivariate statistical analyses: cluster and principal component analysis (PCA), Based on cluster analysis cultivars divided into three major groups and the clustering pattern was related to the classical eco-geographical grouping: occidentalis, pontica and orientalis. Beside geographic origin clustering position of cultivars throughout the dendrogram was related to main uses. The major part (70.1%) of the total variation presented was explained by four principal components. PC1 is highly correlated with the bunch and berry size and PC2 with the density of prostrate hairs of young shoot tip and the density of prostrate hairs between veins on the lower side of mature leaf. The overall arrangement of cultivars suggests considerable phenotypical (and presumably genetic) variability in studied germplasm collection. Furthermore, obtained results may be useful for further utilization of available genetic resources in gene bank managers, growing and breeding.

Key words: amphelographic characteristics, cluster analysis, germplasm, PCA, Vitis vinifera.

Corresponding author: Zoran Bešlić, Agricultural faculty, Nemanjina 6, 11080 Belgrade, Serbia,Tel.: +381 11 2615315; fax: +381 11 3161987,E-mail address: zbeslic@agrif.bg.ac.rs

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Published in „GENETIKA“ Vol. 46, No.3 (2014), pp. 731-744

Serbia

UDC 575:630
DOI: :10.2298/GENSR1403731A

Original scientific paper

IRAP and REMAP-based assessment of genetic diversity in chickpea collection from Iran

Zahra Aghaali¹, Morteza Ghadmizadeh¹, Babak Abdollahi Mandoulakani^*1,2, Iraj Bernousi¹

¹ Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Urmia University, Urmia, Iran.

²Department of Agricultural Biotechnology, Institute of Biotechnology, Urmia University, Urmia, Iran

Abstract

Aghaali Z, M.Ghadmizadeh, B. Abdollahi Mandoulakani, and I. Bernousi (2014): IRAP and REMAP-based assessment of genetic diversity in chickpea collection from Iran. - Genetika, Vol 46, No. 3, 731- 744.

Retrotransposons (RTN) make a significant contribution to the size, organization and genetic diversity of their host genomes. Several RTN families have been identified in chickpea (Cicer arietinum L.) and other closely related species. In the current research, integration activity and insertional polymorphism of the RTNs CARE1, Tms1Ret1, TPS and LORE were studied in 64 chickpea accessions collected in Iran using inter retrotransposon amplified polymorphism (IRAP) and retrotransposon-microsatellite amplified polymorphism (REMAP) techniques. Results indicated that all RTNs studied, are transpositionally active in chickpea genome and amplified scorable and polymorphic banding pattern. Among the RTN families used, the highest percentage of polymorphic loci (PPL) was produced by TPS family (81.82%). Totally, 129 loci were amplified using 18 IRAP and REMAP primers which 83 (64.34%) were polymorphic. The Dice genetic similarity coefficients among accessions ranged from 0.84 (accessions Tj48 and Ba4) to 0.98 (accessions Ka30 and Urm61), averaging 0.93. The parameters of expected heterozygosity (He), Shannon’s information index (I) and number of effective alleles (Ne) were the highest for Urmia accessions. Cluster analysis based on UPGMA algorithm and Dice similarity coefficient categorized the 64 accessions in 7 main groups. The mean Fst values of all groups except for groups IV and VII, were lower than 0.20, demonstrating no clear differentiation among the groups, no genetic structure of the studied chickpea collection and probably occurrences of gene flow among the origins. In conclusion, although RTN-based markers were able to differentiate the chickpea accessions but the measured relative genetic similarity among accessions were not correlated with geographical distances between places of their origins.

Key words: Bayesian analysis, CARE1 retrotransposon, Cicer arietinum L., Genetic diversity

Corresponding author: Babak Abdollahi Mandoulakani, Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Urmia University, Urmia, Iran. Tel: +989122386990 Fax: +984412779558, *Corresponding author’s E-mail: b.abdollahi@urmia.ac.ir

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Published in „GENETIKA“ Vol. 46, No.3 (2014), pp. 745-762

Serbia

UDC 575:630
DOI: 10.2298/GENSR1403745A

Original scientific paper

Molecular tools for utilization of mitochondrial diversity in faba bean (Vicia faba)

Jelena M. ALEKSIĆ¹, Jovanka MILJUŠ-ĐUKIĆ¹, Živko JOVANOVIĆ¹, Branko TOMIĆ² and Bojana BANOVIĆ¹

¹ - Laboratory for Plant Molecular Biology, Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade, Belgrade, Serbia

² - Laboratory for Molecular Biology, Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade, Belgrade, Serbia

Abstract

Aleksić M. J., J. Miljuš-Đukić, Ž.Jovanović, B. Tomić and B. Banović (2014): Molecular tools for utilization of mitochondrial diversity in faba bean (Vicia faba). - Genetika, Vol 46, No. 3, 745- 762.

We performed in silico PCR analyses utilizing complete mitochondrial (mtDNA) genome sequences of faba bean (Vicia faba) and two related species, Vigna angularis and Vigna radiata, currently available in GenBank, to infer whether 15 published universal primer pairs for amplification of all 14 cis-spliced introns in genes of NADH subunits (nad genes) are suitable for V. faba and related species. Then, we tested via PCR reactions whether seven out of 15 primer pairs would generate PCR products suitable for further manipulation in 16 genotypes of V. faba representing all botanical varieties of this species (major, minor, equina and subsp. paucijuga) of various levels of improvement (traditional and improved cultivars) originating from Europe, Africa, Asia and south America. We provide new PCR primers for amplification of nad1 intron 2/3 in V. faba, and demonstrate intraspecific variability in primary nucleotide sequences at this locus. Based on outcomes of both in silico predictions and PCR amplification, we report a set of PCR primers for amplification of five introns in nad genes that are promising molecular tools for future phylogeographic and other studies in this species for which unambiguous data on wild ancestors, centre of origin and domestication are lacking.

Key words: mitochondrial genome, NADH dehydrogenase, introns, in silico PCR, universal primers, faba bean

Corresponding author: Jelena M. Aleksić, Laboratory for Plant Molecular Biology, Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade, Vojvode Stepe 444a, PO box 23, 11010 Belgrade, Serbia, E-mail: aleksic_jelena@yahoo.com.au, jelena.aleksic@imgge.bg.ac.rs

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Published in „GENETIKA“ Vol. 46, No. 3 (2014), pp. 763 - 774

Serbia

UDC 575

DOI: 10.2298/GENSR1403763K

Original scientific paper

GENETIC DIVERSITY OF WHEAT GRAIN QUALITY AND DETERMINATION THE BEST CLUSTERING TECHNIQUE AND DATA TYPE FOR DIVERSITY ASSESSMENT

Mostafa KHODADADI¹, Hamid DEHGHANI^{1, *}, Mohammad Hossein FOTOKIAN²

¹Plant Breeding and Biotechnology Department, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran

²Plant Breeding and Agronomy Department, Faculty of Agriculture, Shahed University, Tehran, Iran

Abstract

Khodadadi M., H. Dehghani, and M. H. Fotokian (2014): Genetic diversity of wheat grain quality and determination the best clustering technique and data type for diversity assessment - Genetika, Vol 46, No. 3, 763- 774

Wheat is an important staple in human nutrition and improvement of its grain quality characters will have high impact on population's health. The objectives of this study were assessing variation of some grain quality characteristics in the Iranian wheat genotypes and identify the best type of data and clustering method for grouping genotypes. In this study 30 spring wheat genotypes were cultivated through randomized complete block design with three replications in 2009 and 2010 years. High significant difference among genotypes for all traits except for Sulfate, K, Br and Cl content, also deference among two years mean for all traits were no significant. Meanwhile there were significant interaction between year and genotype for all traits except Sulfate and F content. Mean values for crude protein, Zn, Fe and Ca in Mahdavi, Falat, Star, Sistan genotypes were the highest. The Ca and Br content showed the highest and the lowest broadcast heritability respectively. In this study indicated that the Root Mean Square Standard Deviation is efficient than R Squared and R Squared efficient than Semi Partial R Squared criteria for determining the best clustering technique. Also Ward method and canonical scores identified as the best clustering method and data type for grouping genotypes, respectively. Genotypes were grouped into six completely separate clusters and Roshan, Niknejad and Star genotypes from the fourth, fifth and sixth clusters had high grain quality characters in overall.

Key words: Genetic Diversity, Iron, Wheat, REML, Zinc

Corresponding author: Hamid Dehghani, Plant Breeding and Biotechnology Department, Faculty of Agriculture, Tarbiat Modares University, Tehran, P.O. Box 14115-336, Iran , Email: dehghanr@modares.ac.ir

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Published in „GENETIKA“ Vol. 46, No. 3 (2014), pp. 775- 788

Serbia

UDC 575:633.15

DOI: 10.2298/GENSR1403775A

Original scientific paper

ESTIMATION OF DROUGHT TOLERANCE AMONG MAIZE LANDRACES FROM MINI-CORE COLLECTION

VioletaANDJELKOVIC, Natalija KRAVIC, Vojka BABIC, Dragana IGNJATOVIC-MICIC, Zoran DUMANOVIC, Jelena VANCETOVIC

Maize Research Institute Zemun Polje, Belgrade, Serbia

Abstract

Andjelkovic V., N. Kravic, V. Babic, D. Ignjatovic-Micic, Z. Dumanovic, and J. Vancetovic (2014): Estimation of drought tolerance among maize landraces from mini-core collection.- Genetika, Vol 46, No. 3, 775- 788

Global climate change, its impact on stable food production in the future and possibilities to overcome the problem are the major priorities for research. Breeding varieties with increase adaptability to changing environments, together with better tolerance/resistance to abiotic stress, pest and diseases are possible solution. Maize is one of the most important crops, with high grain yield reduction induced by drought stress. In the present study twenty-six maize landraces from drought tolerant mini-core collection were tested under optimal, drought, and a combination of drought and high density stresses in the field. Morphological traits, plant height, total number of leaves, leaf length, leaf width, anthesis-silking interval and grain yield were recorded for each entry in two replications in three experiments. Besides, drought tolerant indices were evaluated to test the ability to separate more drought tolerant accessions from those with less stress tolerance. Five stress tolerance indices, including stress tolerance index (STI), mean productivity (MP), geometric mean productivity (GMP), stress susceptibility (SSI), and stress tolerance (TOL) were calculated. Data analyses revealed that STI, MP and GMP had positive and significant correlations with grain yield under all conditions. Three-dimensional diagrams displayed assignment of landraces L25, L1, L14, L3, L26, L15 and L16 to group A, based on the stress tolerance index and achieved grain yield under optimal, drought stress, and a combination of drought and high density stress. A biplot analysis efficiently separated groups of landraces with different level of drought tolerance and grain yield. Based on all obtained results, maize landraces L25, L14, L1 and L3, as the most valuable source of drought tolerance, could be recommended for further use in breeding programs.

Key words: biplot, drought indices, grain yield, landraces, Zea mays L.

Corresponding author: Violeta Andjelkovic, Maize Research Institute Zemun Polje, Slobodana Bajica 1, 11185 Belgrade, Serbia. Tel: +381 11 3756 704, Fax: +381 11 3756 707, avioleta@mrizp.rs

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Published in „GENETIKA“ Vol. 46, No.3 (2014), pp. 789 -797

Serbia

UDC 575
DOI: 10.2298/GENSR1403789J

Original scientific paper

DEVELOPMENT OF 15 GENIC-SSR MARKERS IN OIL-TEA TREE (Camellia oleifera) BASED ON TRANSCRIPTOME SEQUENCING

Baoguang JIA^1,2, Qing LIN^1,2, Lin ZHANG^1,2,*, Xiaofeng TAN^1,2, Xiaolin LEI³, Xiaoyi HU⁴, Fenggong SHAO^1,2

¹Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha, China

²Cooperative Innovation Center of Cultivation and Utilization for Non-Wood Forest Trees of Hunan Province, Central South University of Forestry and Technology, Changsha, China

³Jiangxi Academy of Forestry, Nanchang, China

⁴School of Forestry and Landscape Architecture, Anhui Agricultural University, Hefei, China

Abstract

Jia B., Q.Lin, L. Zhang, X. Tan, X. Lei, X. Hu, and F. Shao (2014): Development of 15 genic-SSR markers in oil-tea tree (Camellia oleifera) based on transcriptome sequencing- Genetika, Vol 46, No. 3, 789-797

Oil-tea tree is one of the most important woody edible oil plants; however, lack of useful molecular markers hinders current genetic research. We performed transcriptome sequencing of developing seeds and characterized microsatellites from transcriptome sequences to identify valuable markers for C. oleifera molecular genetics research. A total of 69,798 unigenes were identified, in which 6,949 putative SSR motifs from 6,042 SSR-containing unique putative transcripts were discovered. Twenty-nine primer pairs corresponding to 29 unigene loci were designed, of which 15 polymorphic genic-SSR markers were developed in 18 varieties and characterized by capillary electrophoresis. The number of alleles per locus (N_a) ranged from 2 to 14, the expected heterozygosity (H_e) ranged from 0.374 to 0.876, and the polymorphism information content (PIC) values ranged from 0.498 to 0.887, respectively. Cross-species amplification was also conducted in 15 varieties of C. japonica. All 15 markers successfully amplified PCR products with expected size in C. japonica and exhibited polymorphisms. The 15 polymorphic genic-SSR markers will have potential for applications in genetic diversity evaluation, molecular fingerprinting identification, comparative genome analysis, and genetic mapping in the C. oleifera and C. japonica.

Key words: Camellia oleifera, transcriptome sequencing, unigene, genic-SSR, cross-species amplification

Corresponding author: Lin Zhang^,498 South Shaoshan Road, Changsha, Hunan 410004, China, Tel: +86-731-5623416; Fax: +86-731-5623406, Email: triwoodtim918@126.com

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Published in „GENETIKA“ Vol. 46, No. 3(2014), pp. 799-806

Serbia

UDC 575.630.11
DOI: 10.2298/GENSR1403799B

Original scientific paper

GENOTYPE × ENVIRONMENT INTERACTIONS AND PHENOTYPIC STABILITY FOR WHEAT GROWN IN STRESSFUL CONDITIONS

Borislav BANJAC^*, Velimir MLADENOV, Miodrag DIMITRIJEVIĆ, Sofija PETROVIĆ,

Jan BOĆANSKI

University of Novi Sad, Faculty of Agriculture Novi Sad

Abstract

Banjac B., V. Mladenov, M. Dimitrijević, S. Petrović, and J. Boćanski (2014): Genotype × environment interactions and phenotypic stability for wheat grown in stressful conditions- Genetika, Vol 46, No. 3, 799-806

The objective of this study was to present the results of experiment conducted on 11 cultivars of wheat (Triticum aestivum L.) and 1 cultivar of triticale (Triticosecale W) on stressful conditions of halomorphic solonetz in Kumane, Banat, Serbia. Across three growing seasons genotypic variability, monitoring of phenotypic variation and genotype by environment interaction (GEI) for number of grains per spike and yield was studied. The cultivar were grown in field trails of control treatment and treatments with measures repairs solonetz using phosphogypsum in the amount of 25 t∙ha^-1 and 50 t∙ha^-1. GEI was tested using AMMI (Additive Main Effects and Multiplicative Interaction) model. The expression of tested traits were statistically significant and showed additive and non-additive sources of variation. The first source of variation, quantified IPCA₁ axis explained most of the structure of GEI.

Key words: AMMI, interaction, solonetz, wheat

Corresponding author: Borislav Banjac, University of Novi Sad, Faculty of Agriculture Novi Sad, Sq. Dositeja Obradovića 8, 21 000 Novi Sad, phone: +381 (21) 485 32 99; e-mail:borislav.banjac@polj.edu.rs

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Published in „GENETIKA“ Vol. 46, No.3(2014), pp. 807- 813

Serbia

UDC 575:630.15
DOI: 10.2298/GENSR1403807K

Original scientific paper

HETEROSIS AND ANTIOXIDANT COMPOUNDS OF SWEET CORN BREEDING LINES AND THEIR F₁ HYBRID

Boris KRAPTCHEV*, Roumiana VASSILEVSKA-IVANOVA, Lydia SHTEREVA

Department of Applied Genetics and Biotechnology, Institute of Plant Physiology

and Genetics, Bulgarian Academy of Sciences, Sofia, Bulgaria

Abstract

Kraptchev B., R. Vassilevska-Ivanova, L. Shtereva (2014): Heterosis and antioxidant compounds of sweet corn breeding lines and their F₁ hybrid- Genetika, Vol 46, No. 3, 807-813

In this research, the mid-parent heterosis and the levels of phenolic, flavonoids compounds and antioxidants activity among two selected sweet corn parental lines and their F₁ hybrid Zaharina were evaluated. Substantial positive mid-parent heterosis (MPH) was found for parent-hybrid triplet for ear weight and for plant height. Among all other traits, insertion height had the greatest heterosis and ear diameter had the lowest. Negative mid-parent heterosis exhibited only the trait 1000-kernel weight. Data analysis indicated significant differences in the contents of total phenolic content, total flavonoids content, water–soluble antioxidant capacity, lipid-soluble antioxidant capacity, and antioxidant activity among parent-hybrid triplet. The results suggest that F₁ hybrid Zaharina can be considered as good source of natural antioxidants since it extracts were found to possess high antioxidant activity.

Key words: α-tocopherol; ascorbate; flavonoids; phenols; sweet corn; Z ea mays

Corresponding author: Stanislava Grozeva, Department of Breeding, Variety Maintenance and Introduction, Maritsa Vegetable Crops Research Institute, Brezovsko shosse 32, 4003 Plovdiv, Bulgaria, Tel.: +35932952296, Fax: +35932960177, e-mail: stanislava_grozeva@abv.bg;

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Published in „GENETIKA“ Vol. 46, No. 3(2014), pp. 815- 829

Serbia

UDC 575:630

DOI: 10.2298/GENSR1403815B

Original scientific paper

SEQUENTIAL PATH ANALYSIS FOR DETERMINING INTERRELATIONSHIPS BETWEEN YIELD AND RELATED TRAITS IN TOBACCO (Nicotiana tabacum L.) UNDER NORMAL AND ABIOTIC STRESS CONDITIONS

Mahdi BAYAT¹, Reza DARVISHZADEH^2,3,*, Farough SOLEIMANI¹, Seyyed Reza ALAVI⁴

¹Department of Agronomy, Urmia University, Urmia, Iran.

²Department of Plant Breeding and Biotechnology, Urmia University, Urmia, Iran.

³Institute of Biotechnology, Urmia University, Urmia, Iran.

⁴Department of Genetic, Urmia Tobacco Research Centre, Urmia, Iran.

Abstract

Bayat M., R. Darvishzadeh, F. Soleimani, and S. Reza Alavi (2014): Sequential path analysis for determining interrelationships between yield and related traits in tobacco (Nicotiana tabacum L.) under normal and abiotic stress conditions- Genetika, Vol 46, No. 3, 815-829

In the present work the relationships between yield and its related traits were investigated in tobacco genotypes under normal and abiotic stress conditions (Orobanche aegyptiaca weed) at Urmia Tobacco Research Centre, Iran, during 2006-2009 cropping seasons. The experimental design was a randomized complete block design (RCBD) with three replications in each condition every year. Analysis of variance revealed extent genetic variability among the genotypes for most of the traits studied. In comparison with normal condition, the mean value of studied traits decreased in stress condition. LAI and FD showed the maximum and minimum diminution in the mean values under stress condition compared to normal one so known as more sensitive and more tolerant traits, respectively. Based on CV values, the traits FD and DLYP showed the minimum and maximum variation among traits in both normal and stress conditions. Correlation analysis revealed significant and positive correlations between DLYP with all studied traits in both normal and stress conditions. Path analysis detected the traits including biomass, APDW and DWR as the first-order variables at normal condition and biomass, APDW, DWR and harvest index as the first-order variables under abiotic stress condition. Based on results, the traits such as biomass, APDW, DWR detected as more important factors in both conditions can be used in tobacco breeding programs for increasing yield.

Abbreviation: aerial part fresh weight without leaves weight (APFW), aerial part dry weight without leaves weight (APDW), biomass (BIO), coefficient of variation (CV), dry weight of root (DWR), flowering date (FD), fresh weight of leaf (FWL), fresh weight of root (FWR), harvest index (HI), leaf area index (LAI), dry leaf yield per plant (DLYP), number of leaf (NL), plant height (PH), randomized complete block design (RCBD), standard deviation (Std).

Key words: direct effect, indirect selection, multicollinearity, path analysis, predictor variables

Corresponding author: R. Darvishzadeh, Department of Plant Breeding and Biotechnology, Urmia University, Urmia, Iran. E-mail: r.darvishzadeh@urmia.ac.ir. Phone: + 98 914 9734458. Fax: + 98 44 32779558

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Published in „GENETIKA“ Vol. 46, No. 3(2014), pp. 831- 838

Serbia

UDC 575:633
DOI: 10.2298/GENSR1403831J
Original scientific paper

GENETIC VARIABILITY AND ASSOCIATION OF YIELD ATTRIBUTING TRAITS WITH GRAIN YIELD IN UPLAND RICE

Nitiprasad Namdeorao JAMBHULKAR^* and Lotan Kumar BOSE

Central Rice Research Institute, Cuttack-753006, Orissa, India

Abstract

Jambhulkar N. N. and L. K. Bose (2014): Genetic variability and association of yield attributing traits with grain yield in upland rice- Genetika, Vol 46, No. 3, 831-838

A study was undertaken to find out the genetic variability and correlation between yield and other yield attributing characters of rice genotypes in upland ecology. The experiment was conducted with twenty two upland genotypes grown during Wet season under direct seeded condition in a randomized block design. Analysis of variance shows significance in all the traits indicating the presence of considerable amount of genetic variation among the genotypes. The traits like plant height, ear bearing tiller and yield has high genotypic coefficient of variance, phenotypic coefficient of variance and genetic advance. Plant height, ear bearing tiller, panicle length, panicle weight and length were positively and significantly correlated with yield. Plant height and grain per panicle, 50% flowering, thousand grain weight and ear bearing tiller has high direct effect on yield. Therefore, selection based on plant height, 50% flowering, thousand grain weight and ear bearing tiller could be more effective in upland rice.

Key words: correlation, direct effect, genetic advance, heritability, path analysis, rice

Corresponding author: Nitiprasad Namdeorao Jambhulkar, Central Rice Research Institute, Cuttack – 753006, India. Email: nitiprasad1@gmail.com

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Published in „GENETIKA“ Vol. 46, No. 3(2014), pp. 839-854

S. Bajića 1, 11185 Belgrade-Zemun

Serbia

UDC 575:633

DOI: 10.2298/GENSR1403839P

Original scientific paper

Genetic relatedness of soybean genotypes based on agromorphological traits and rapd markers

Vesna PERIĆ¹, Ana NIKOLIĆ¹, Vojka BABIĆ¹, Aleksanda SUDARIĆ², Mirjana SREBRIĆ¹,

Vuk ĐORĐEVIĆ³, Snežana MLADENOVIĆ DRINIĆ¹

¹ Maize Research Institute “Zemun Polje”, Belgrade, Serbia

² Agricultural Institute Osijek, Osijek, Croatia

³ Institute of Field and Vegetable Crops, Novi Sad, Serbia

Abstract

Perić V., A. Nikolić, V. Babić, A. Sudarić, M. Srebrić, V.Đorđević, and S. Mladenović Drinić (2014): Genetic relatedness of soybean genotypes based on agromorphological traits and rapd markers- Genetika, Vol 46, No. 839- 854

Modern agriculture, breeding procedures, as well as competition among breeding institutions contribute to further reduction of already narrowed diversity of soybean commercial varieties. The objective of the study was to characterize eighteen soybean cultivars from three different breeding programs for agro-morphological traits and to reveal genetic diversity using molecular markers. Morphological description was performed with 13 qualitative and 9 quantitative traits. The genetic relationships were estimated using 21 RAPD markers. PIC was calculated for RAPD data, while the diversity of qualitative traits was described by Shannon genetic diversity index. Cluster analysis based on qualitative morphological characters showed clear separation of genotypes on the basis of their plant growth type. PC analysis performed for quantitative traits divided genotypes according to their maturity group. Grouping pattern based on molecular marker data was in agreement with pedigree of cultivars. A great similarity was found, primarily between the varieties under the same institution, and then among all examined varieties. Comparison of three methods in the assessment of diversity indicated that morphological markers might provide useful information in breeding process and allow classification by pedigree to some extent, but RAPD markers were found to be superior in assessing differences among genetically very similar genotypes.

Key words: agronomic traits, diversity, RAPD markers, soybean

Corresponding author: Vesna Perić, Maize Research Institute “Zemun Polje”, Slobodana Bajića 1, 11185 Zemun Polje, Serbia, E-mail:vperic@mrizp.rs, Phone: + 381 64 840 6235

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Published in „GENETIKA“ Vol. 46, No. 3(2014), pp. 855-864

S. Bajića 1, 11185 Belgrade-Zemun

Serbia

UDC 575:630

DOI: 10.2298/GENSR1403855Z

Original scientific paper

STANDARDIZATION AND REPRODUCIBILITY OF RANDOM MARKER BASED ANALYSIS OF MICROPROPAGATED CRIMSON BEEBALM

Jana ŽIAROVSKÁ¹*, Milan BEŽO¹, Michaela HRDLIČKOVÁ², Eloy FERNANDÉZ²

¹ Department of Genetics and Plant Breeding, Faculty of Agrobiology and Food Resources, Slovak University of Agriculture in Nitra, Slovak Republic

² Department of Crop Sciences and Agroforestry, Faculty of Tropical AgriSciences, Czech University of Life Sciences in Prague, Czech Republic

Abstract

Žiarovská J. M. Bežo, M. Hrdličková, and E. Fernandéz(2014): Standardization and reproducibility of random marker based analysis of micropropagated crimson beebalm- Genetika, Vol 46, No. 3, 855-864

Genetic analysis of the stability in micropropagated plant material are required to be perform by effective and reproducible techniques. When no specific DNA based markers are available for certain species, such as for crimson beebalm, standardized and optimized protocols can be used even for random based markers. Here we report for the first time the optimization of PCR conditions for RAPD analysis for the purposes of the establishment of fast, reliable and reproducible method for screening of genetic stability in Monarda dydima, L. micropropagated cultures. RAPD protocol was optimized based on the use of 50 or 100 ng of template DNA and annealing temperature of 36°C, resulted optimal amplification. Setting the standardized PCR conditions, primers used in experiments generated reproducible and distinguishable fingerprinting patterns for micropropagated cultures of crimson beebalm and is suitable for further analysis of studying genetic variation of this medicinal plant.

Key words: crimson beebalm, Monarda dydima, L., RAPD optimization, reproducibility

Corresponding author: Jana Žiarovská, Department of Genetics and Plant Breeding, Tr. A. Hlinku 2, 94976, Nitra, Slovak Republic; e-mail: jana.ziarovska@uniag.sk

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Published in „GENETIKA“ Vol. 46, No. 3(2014), pp. 865-875

Serbia

UDC 575

DOI: 10.2298/GENSR1403865M

Original scientific paper

Analysis of genetic structure in Slovak Pinzgau cattle using five candidate genes related to milk production traits

Martina MILUCHOVA, Michal GABOR, Anna TRAKOVICKA

Department of Genetics and Breeding Biology, Slovak University of Agriculture in Nitra, Slovakia

Abstract

Miluchova M., M. Gabor, and A. Trakovicka (2014): Analysis of genetic structure in Slovak pinzgau cattle using five candidate genes related to milk production traits- Genetika, Vol 46, No. 3, 865-875

The goal of the paper was to identify genetic structure of five candidate genes for milk production in Slovak Pinzgau breed. A total of 86 mothers of bulls of Slovak Pinzgau cattle were use in this study. To genotype of cows for candidate genes we used PCR methods (PCR-RFLP, ARMS–PCR, multiplex PCR-RFLP). On the basis of PCR analyses we established genotype structure of cattle population and calculated allelic frequencies. Effectiveness of allele incidence and genetic diversity was evaluated with following parameters: theoretical heterozygosity (He exp), experimental heterozygosity (He obs), polymorphism information content (PIC), expected homozygosity (E), effective number of alleles (ENA), level of possible variability realization (V%). Slovak Pinzgau cattle exhibit the high values of heterozygosity, polymorphism information content, effective number of alleles and level of possible variability realization for genes CSN2, CSN3 and LALBA. In opposite, for genes CSN1S1 and LGB show high values of homozygosity.

Key words: candidate genes; genetic structure, milk production; Slovak Pinzgau cattle

Corresponding author: Martina Miluchova, Department of Genetics and Breeding Biology, Slovak University of Agriculture in Nitra, 949 76 – Nitra, Tr. A Hlinku, 2, Slovakia Tel: 00421-6414290, e-mail: martina.miluchova@centrum.sk, martina.miluchova@uniag.sk

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Published in „GENETIKA“ Vol. 46, No. 3(2014), pp. 877-882

Serbia

UDC 575

DOI: 10.2298/GENSR1403877F

Original scientific paper

HIGH RESOLUTION KARYOTYPE OF THAI CRAB-EATING MACAQUE (Macaca fascicularis)

Xiaobo FAN¹, Alongkoad TANOMTONG², Arunrat CHAVEERACH², Krit PINTHONG ^{1,2 3,} Siripiyasing PORNNARONG^1,2, Weerayuth SUPIWONG^1,2, Thomas LIEHR¹, Anja WEISE¹

¹Jena University Hospital, Friedrich Schiller University, Institute of Human Genetics, Jena, Germany

²Department of Biology Faculty of Science, Khon Kaen University, Muang District, Khon Kaen, Thailand

³Faculty of Science and Technology, Surindra Rajabhat University, Maung District, Surin, Thailand

Abstract

Fan X., A. Tanomtong, A. Chaveerach, K. PInthong,^, S. Pornnarong, W. Supiwong, T. Liehr, and Anja Weise (2014): High resolution karyotype of thai crab-eating macaque (Macaca fascicularis) - Genetika, Vol 46, No. 3, 877-882

Comparative chromosome banding analysis and/or fluorescence in situ hybridization (FISH) studies are established approaches to compare human and ape chromosomes. FISH-banding is a relatively new and not routinely applied method suited very well to provide to a better understanding of the evolutionary history of primate and human phylogeny. Here multicolor banding (MCB) applying probes derived from Homo sapiens was used to analyze the chromosomes of Thai crab-eating macaque (Macaca fascicularis). The results agree with those of previous studies in other macaques, e.g. Macaca sylvanus or Macaca nemestrina. This result pinpoints, that morphological differences within the Ceropithecoidae must be founded rather in subchromosomal changes or even in epigenetics than in gross structural alterations.

Key words: fluorescence in situ hybridization (FISH), karyotype, Macaca fascicularis, multicolor banding,

Corresponding author: Thomas Liehr, Institut für Humangenetik, Postfach D-07740 Jena, Germany,Tel: ++49-3641-935533, Fax. ++49-3641-935582, email: Thomas.Liehr@med.uni-jena.de

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Published in „GENETIKA“ Vol. 46, No. 3(2014), pp. 883-894

Serbia

UDC 575
DOI: 10.2298/GENSR1403883M

Original scientific paper

Testing the adaptive plasticity of gypsy moth digestive enzymes in response to tannic acid using phenotypic selection analysis

Marija Mrdaković^1*, Biljana Stojković^{2, 3}, Larisa Ilijin¹, Milena Vlahović¹,

Vesna Perić-Mataruga¹, Jelica Lazarević¹

¹Department of Insect Physiology and Biochemistry, Institute for Biological Research “Siniša Stanković”, University of Belgrade, Belgrade, Serbia

²Department of Evolutionary Biology, Institute for Biological Research “Siniša Stanković”, University of Belgrade, Belgrade, Serbia

³Faculty of Biology, University of Belgrade, Belgrade, Serbia

Abstract

Mrdaković M., .B. Stojković, L. Ilijin, M. Vlahović, V. Perić-Mataruga, and J. Lazarević (2014): Testing the adaptive plasticity of gypsy moth digestive enzymes in response to tannic acid using phenotypic selection analysis - Genetika, Vol 46, No. 3, 883-894

The adaptive significance of plasticity of digestive enzyme responses to allelochemical stress was tested on 32 full-sib gypsy moth families from an oak forest (the Quercus population) and 26 families from a locust-tree forest (the Robinia population), reared on control or tannic acid-supplemented diets. By using the relative growth rate as a fitness measure in phenotypic selection analyses, we revealed that higher specific activity of leucine aminopeptidase in Quercus larvae and lower specific activity of trypsin in Robinia larvae were adaptive in the control environment. In Quercus larvae, elevated specific activities of leucine aminopeptidase and lipase were adaptive in the stressful environment. There were no plasticity costs for the enzyme activities in either experimental group. The obtained results suggest that adaptive plasticity of digestive enzyme activity in gypsy moth larvae contributes to optimal growth rate under various environmental conditions.

Key words: fluorescence in situ hybridization (FISH), karyotype, allelochemical stress, digestive enzymes, Lymantria dispar L., relative growth rate, selection gradients

Corresponding author: Marija Mrdaković, Department of Insect Physiology and Biochemistry, Institute for Biological Research “Siniša Stanković”, University of Belgrade, Belgrade, Serbia, email: mm1507@ibiss.bg.ac.rs

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Published in „GENETIKA“ Vol. 46, No. 3(2014), pp. 895-904

Serbia

UDC 575

DOI: 10.2298/GENSR1403895O

Original scientific paper

THE “POTATO ROAD” AND BIOGEOGRAPHIC HISTORY OF POTATO CYST NEMATODE POPULATIONS FROM DIFFERENT CONTINENTS

Violeta oro¹, Bogdan nikolić¹ and Dragana jošić²

¹Institute of Plant Protection and Environment, Belgrade, Serbia

² Institute of soil science, Belgrade, Serbia

Abstract

Oro V., B. Nikolić and D. Jošić (2014):The “potato road” and biogeographic history of potato cyst nematode populations from different continents. - Genetika, Vol 46, No. 3,895 -904.

The general opinion about the introduction of potato in Europe is the one regarding the direction from South America to Spain and subsequent distribution to other continents. Some historical data point out an alternative road. The potato spread from its place of origin to other continents in the light of parasite-host relationship, relying on nematode molecular data, is discussed in the present work. Biogeographic history of potato cyst nematode populations from different continents is in congruence with historical records.

Key words: biogeography, potato, potato cyst nematodes

Corresponding author: Violeta Oro, Institute for Plant Protection and Environment, Teodora Drajzera 9, Belgrade, Serbia, Phone: 381 11 2660 049, e-mail: viooro@yahoo.com

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Published in „GENETIKA“ Vol. 46, No. 3(2014), pp. 905-914

Serbia

UDC 575

DOI: 10.2298/GENSR1403905S

Original scientific paper

CORRELATION AND PATH COEFFICIENT ANALYSIS OF YIELD AND YIELD COMPONENT IN SOME OF BROAD BEAN (Vicia faba L.) GENOTYPES

Peyman SHARIFI

Department of Agronomy and Plant Breeding, Rasht Branch, Islamic Azad University, Rasht, Iran

Abstract

Peyman Sharifi (2014): Correlation and path coefficient analysis of yield and yield component in some of broad bean (Vicia faba L.) genotypes- Genetika, Vol 46, No. 3, 905 - 914.

Faba bean is a grain legume and grown for its high protein content in the seed. It is also serves as a rotational crop which play great role in controlling disease epidemics in areas were cereal mono-cropping is abundant. Yield in faba bean, similar to the other crops, is a complex trait and constitute by many of morphological and physiological traits. This study was carried out during 2011-12 and 2012-13 in two region of Iran including Guilan and Lorestan provinces. Field experiments were conducted in a randomized complete block design with three replications and ten genotypes. The results of combined analysis of variance indicated that the studied genotypes differed significantly for all of the studied traits. The results indicated also environment effect and environment × genotype interaction effects were significant or highly significant for all of the traits. The highest seed yield were determined for genotype 1 (3159.9 and 4016.9 kg ha^-1 at 2012 and 2013, respectively) in Guilan and genotype 5 (495.44 kg ha^-1) in Lorestan. The results of correlation analysis indicated that there were positive significant correlation coefficients between seed yield and seed length (LS), seed width (WS), pod length (PL) and hundred seed weight (HSW) in Guilan province at two cropping season. Path coefficient analysis indicated that traits containing number of pod per plant, number of steam per plant, pod length, seed length/width ratio and hundred seed weight had the highest positive direct effects on dry seed yield in studied faba bean genotypes. Attention should be paid to some of characters such as pod length, hundred seed weight, number of pods per plant and number of stems per plant for augmentation of seed yield and these traits could be used as selection criteria in faba bean breeding programs. These findings indicate that selection for each or full of the above traits would be accompanied by high yielding ability under such conditions. It could be concluded that the high yielding genotypes, such as 1 and 2 could be used to improve faba beans and making possibilities of extending production of this legume crop.

Key words: breeding, correlation, Vicia faba,L. locally landraces

Corresponding author: Abdulmojeed Yakubu,Department of Animal Science, Faculty of Agriculture, Nasarawa State University, Keffi, Shabu-Lafia Campus, , Lafia, Nasarawa State, Nigeria, Email: abdulmojyak@gmail.com

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Published in „GENETIKA“ Vol. 46, No. 3(2014), pp 915-924

Serbia

UDC 575:633.15
DOI: 10.2298/GENSR1403915S

Original scientific paper

CHARACTERIZATION OF CORNELIAN CHERRY (Cornus mas L.) GENOTYPES - GENETIC RESOURCES FOR FOOD PRODUCTION IN CZECH REPUBLIC

Jiri SOCHOR¹, Tunde JURIKOVA², Sezai ERCISLI^3*, Jiri MLCEK⁴, Mojmir BARON¹, Stefan BALLA², Suzan Ozturk YILMAZ⁵and Thomas NECAS⁶

¹Department of Viticulture and Enology, Faculty of Horticulturae, Mendel University in Brno, Lednice, Czech Republic

²Institut for teacher training , Faculty of Central European Studies, Constantine the Philosopher University in Nitra, Nitra, Slovak Republic

³Department of Horticulture, Ataturk University Erzurum-Turkey

⁴Department of Food Technology and Microbiology, Faculty of Technology, Tomas Bata University in Zlin, Namesti T.G.Masaryka Zlin, Czech Republic

⁵Department of Food Engineering, Sakarya University Sakarya-Turkey

⁶Department of Fruit Growing, Faculty of Horticulture, Mendel University in Brno, Lednice, Czech Republic

Abstract

Sochor J., T. Jurikova, S. Ercisl^*, J.i Mlcek, M. Baron, S. Balla, S. O. Yilmazand T. Necas (2014): Characterization of cornelian cherry (Cornus mas L.) genotypes - genetic resources for food production in Czech Republic- Genetika, Vol 46, No. 3, 915- 924

The aim of the study was to determine and compare both technological properties and polyphenol content in fruits of eight cornellian cherry (Cornus mas L.) cultivars from Czech Republic. The fruits of cultivar ´Vydubeckij´ had the highest dry matter (17.4%) and soluble solid (15.8%) content among searched cultivars. The highest crude protein and phosphorus values were determined in the fruits of the ´Titus´ cultivar as 10.9 g of crude protein and 435 mg of phosphorus for per kgfresh weight (FW) base. The ´Elegantnyj´ and ´Vydubeckij´ cultivars are found to be more suitable for canning industry. The fruits of the ´Vydubeckij´ cultivar contained 10.8 g of pectin and 22.4 g of citric acid per kg fresh weight base, and could be important for technological use because of their excellent gelling ability. ´Devin´,´Vydubeckij´ and ´Titus´ cultivars had the most valuable source of chlorogenic acid (135.6, 110.9 and 115.1 mg per 100 g FW, respectively) and quercetin (24.9, 25.2 and 24.2 mg per 100 g FW, respectively). This work should contribute to the popularization of this fruit specie as a valuable source of technological parameters for human nutrition and the canning industry.

Key words: Cornelian cherry, diversity, fruit properties, industry

Corresponding author: Sezai Ercisli, Department of Horticulture, Ataturk University 25240 Erzurum-Turkey,E-Mail: sercisli@gmail.com,Tel.: +90-442-231-2599; Fax: +90-442-236-0958.

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Published in „GENETIKA“ Vol. 46, No. 3(2014), pp. 925-934

UDC 575
DOI: 10.2298/GENSR1403925Y

Original scientific paper

MOLECULAR ANALYSIS OF SOLUTE CARRIER FAMILY 11 MEMBER A1 (SLC11A1) GENE IN RUMINANTS AND NON-RUMINANTS USING COMPUTATIONAL METHOD

Abdulmojeed YAKUBU^1*, Daniels Ewaoche ALADE¹ and Ndubuisi I. DIM²

¹Department of Animal Science, Faculty of Agriculture, Nasarawa State University, Keffi, Shabu-Lafia Campus, , Lafia, Nasarawa State, Nigeria

²Department of Animal Breeding and Physiology, University of Agriculture, Benue State, Nigeria

Abstract

Yakubu A., D. E. Alade and N. I. Dim (2014): Molecular analysis of solute carrier family 11 member A1 (SLC11A1) gene in ruminants and non-ruminants using computational method- Genetika, Vol 46, No. 3, 925- 934.

Solute carrier family 11 member a1 gene (Slc11a1) previously known as natural resistance-associated macrophage protein 1 (Nramp1) is a gene of member of family of metal ion-transport protein. The cellular expression is restricted to phagocytic cells. Slc11a1 delivers bivalent metal cations from the cytosol into acidic endosomal and lysosomal compartments where Fenon and Haber-Weiss reaction generates toxic antimicrobial radicals for direct antimicrobial activity against harmful microorganisms. The present study was undertaken with the objective of analyzing Slc11a1 gene to gain insight into the evolutionary proximity and divergence as well as the polymorphism of this gene in ruminants and non-ruminants including the attendant effects of the genetic variants on the function of the Slc11a1 protein. Thirty Slc11a1 gene sequences of 6 mammalian species classified as ruminant (goat, sheep, cattle, Bubalus bubalis and Bubalus carabanensis) and non-ruminant (swine and horse) animals were investigated. The length of the Slc11a1 gene varied from 448-2,357. There was substantial genetic variation and polymorphism in the aligned sequences of Slc11a1 gene within and across species. Functional analysis of non-synonymous mutations in cattle revealed that twenty five of the amino acid substitutions at the peptide binding region could be beneficial, (E36G), (T52A), (N161S) and (V248I) were likely to be beneficial while only (Q312K) was harmful. In horse, two of the amino acid mutations were harmful, two were likely to be harmful, one was undecided, four were likely beneficial and the rest twenty were beneficial. The phylogenetic trees showed some form of proximity and differentiation in Slc11a1 sequences within and across species. The present information on the polymorphism of Slc11a1 gene might be exploited in the search for association with disease resistance in Nigerian livestock species.

Key words: functional analysis, mammals, polymorphism, phylogenetic trees.Slc11a1 gene

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Published in „GENETIKA“ Vol. 46, No. 3(2014), pp. 935-948

Serbia

UDC 575:633.15

DOI: 10.2298/GENSR1403935C

Original scientific paper

POLLEN GERMINATION AND POLLEN TUBE GROWTH

IN ZP MAIZE LINES

Radosav CEROVIĆ¹, Zorica PAJIĆ², Milomir FILIPOVIĆ²,Milica FOTIRIĆ-AKŠIĆ³,

Sanja RADIČEVIĆ⁴, Dragan NIKOLIĆ³, Milena ĐORĐEVIĆ⁴

¹University of Belgrade, Innovation Centre, Faculty of Technology and Metallurgy,

Belgrade, Serbia

²Maize Research Institute „Zemun Polje”, Belgrade-Zemun, Serbia

³University of Belgrade, Faculty of Agriculture , Zemun, Serbia

⁴Fruit Research Institute, Čačak, Serbia

Abstract

Cerović,R., Z. Pajić, M. Filipović,M. Fotirić-Akšić, S. Radičević, D. Nikolić, and M. Đorđević (2014): Pollen germination and pollen tube growth in ZP maize linesd- Genetika, Vol 46, No. 3, 935- 948.

The study was conducted on the in vitro pollen germination at 26°, 28°, 32° and 35°C for 24h of male parental lines, pollen tube growth in vivo in cross pollination of female and male parental lines that make couples in four hybrids: ZP 504 su (♀ ZPPL 51 × ♂ ZPPL 67); ZP 677 (♀ ZPPL 17 × ♂ ZPPL 201); ZP 704 (♀ ZPPL 109 × ♂ ZPPL 79), ZP 611 k (♀ ZPPL 126 × ♂ ZPPL 105), and the open pollination of female parental lines of the above mentioned hybrids. Pollen germination in vitro and pollen tube growth dynamics in vivo showed different genotypic specificities with the tests applied. The obtained results were discussed in the context of reproductive biology of ZP maize lines and aimed to create the preconditions for successful management and direction of the process in practice - seed production in certain environmental conditions.

Key words: parental lines; pollen; pollination; pollen tube growth, Zea mays L.

Corresponding author: Radosav Cerović,Innovation Centre, Faculty of Technology and Metallurgy,Karnegijeva 4,11020 Beograd, Serbia,Phone: + 381 65 444 11 55,E-mail: radosav.cerovic@gmail.com

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Published in „GENETIKA“ Vol. 46, No. 3(2014), pp. 949-961

Serbia

UDC 575:630

DOI: 10.2298/GENSR1403949A

Original scientific paper

GENETIC DIVERSITY IN RED CLOVER (Trifolium pratense L.) USING SSR MARKERS

Ramadan Salem AHSYEE¹, SanjaVASILJEVIĆ³, Irena ĆALIĆ², Miroslav ZORIĆ³, Đura KARAGIĆ³, Gordana ŠURLAN-MOMIROVIĆ²

¹El-Gabel El-Garbe University, Faculty of Natural Sciences, Tripoli, Libya.

²University of Belgrade, Faculty of Agriculture, Serbia.

³Institute of Field and Vegetable Crops, Novi Sad, Serbia.

Abstract

Ahsyee R. S., S.Vasiljević, I. Ćalić, M. Zoric, Đ. Karagić, and G. Šurlan-Momirović (2014): Genetic diversity in red clover (Trifolium pratense L.) using SSR markers- Genetika, Vol 46, No. 3, 949- 961.

Red clover (Trifolium pratense L.) is one of the main forage species from temperate regions and it's originated in Eurasia where wild-type populations are found in the Caucasus Mountains. Considerable variation for this crop has been recorded for few molecular variations. The identification and understanding of molecular genetic diversity in red clover accessions will help in effective genetic conservation along with efficient breeding programs in this crop. This study has shown molecular diversity using microsatellite markers in red clover accessions from around of the world. In this investigation, 40 genotypes have been selected and analyzed using 15 SSR primers. These primer pairs amplified 1146 polymorphic loci among the genotypes screened. The number of fragments amplified by each SSR primer combination varied from 24 for RCS3681 to 109 for RCS1729 with an average value of 80.78 per primer combination. The SSR marker data was further analyzed using cluster algorithms and Principal coordinates analysis (PCoA). The results indicated that the considerable genetic variations were discovered among the analyzed genotypes. The SSR based clustering could identify the putative pedigree types of the present red clover types of diverse origins

Key words: cluster analysis, genetic distance, genetic variation, principal coordinates analysis, red clover, SSR molecular markers

Corresponding author: Gordana Šurlan-Momirović,Faculty of Agriculture, Nemanjina 6, 11080 Belgrade-Zemun, Serbia,phone: +381 112615315 / 195,e-mail: surlang@agrif.bg.ac.rs

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Published in „GENETIKA“ Vol. 46, No. 3(2014), pp. 963-973

Serbia

UDC 575:630

DOI: 10.2298/GENSR1403963M

Original scientific paper

GENETIC STRUCTURE OF BLACK POPLAR (Populus nigra L.) POPULATION

IN THE AREA OF GREAT WAR ISLAND

Zoran MAKSIMOVIĆ¹, Dijana ČORTAN², Vladan IVETIĆ²,

Snežana MLADENOVIĆ DRINIĆ³, Mirjana ŠIJAČIĆ-NIKOLIĆ²

¹ State Enterprise for Forest Management “Srbijašume“, Belgrade, Serbia

² University of Belgrade, Faculty of Forestry, Belgrade, Serbia

³Maize Research Institute, Zemun Polje, Belgrade, Serbia

Abstract

Maksimović Z., D. Čortan, V. Ivetić, S. Mladenović Drinić, M. Šijačić-Nikolić (2014): Genetic structure of black poplar (Populus nigra l.) population in the area of Great war island - Genetika, Vol 46, No. 3, 963-973

The genetic structure of black poplar (Populus nigra L.) populations in the area of Great War Island (GWI) was studied at the level of 30 genotypes, based on microsatellite molecular markers (SSR). Eleven polymorphic SSR loci were used for analysis of intarpopulation genetic variability. Observed and expected heterozygosity in studied population were high (0.70 and 0.82). The fixation index calculated for single locus ranged from -0.055 (PMGC_14) up to 0.424 (PMGC_2607), while the mean value was 0.143. Deviation from Hardy-Weinberg equilibrium (HWE) differed between single loci. Stabile genetic structure and satisfactory level of genetic variability that have been determined at the population level represent a good starting point for conservation and sustainable use of the available gene pool and further breeding of this species.

Key words: black poplar, Great War Island, genetic variability, microsatellites

Corresponding author: Zoran Maksimović,State Enterprise for Forest Management “Srbijašume“, Mihajlo Pupin Boulevard 113, 11070 New Belgrade,Tel: +381 64 313 59 62,Fax: +381 11 711 85 13,E-mail: zoran.maksimovic@srbijasume.rs

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Published in „GENETIKA“ Vol. 46, No.3(2014), pp. 975-984

Serbia

UDC 575

DOI: 10.2298/GENSR1403975G

Original scientific paper

ANALYSIS OF GENETIC DIVERSITY OF SUGAR BEET GENOTYPES USING RANDOM AMPLIFIED POLYMORPHIC DNA MARKER

Ali Reza GHASEMI¹, Ahmad Reza GOLPARVAR^1*, Mehdi Nasr ISFAHANI²

¹Department of Agronomy and plant Breeding, College of Agriculture, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran

²Plant Protection Research Division, Agricultural and Natural Resources Research Center, Isfahan 81785-199, Iran

Abstract

Ghasemi A. R., A. R. Golparvar and M. N. Isfahani(2014): Analysis of genetic diversity of sugar beet genotypes using random amplified polymorphic DNA marker- Genetika, Vol 46, No. 3, 975-984

Plant breeding programs are formulated based on the diversity and selection of superior quantitative and qualitative traits. Hence, assessment of genetic diversity is the first step of every plant breeding program. In this regard, use of new methods for studying genetic diversity seems necessary. In the present study, the genetic diversity of thirty sugar beet genotypes was determined using Random Amplified Polymorphic DNA (RAPD) marker. Following the DNA extraction and optimization of experiment conditions, of the 40 primers under study, 10 primers that induced polymorphism and produced good and clear bands in the genotypes of sugar beet were randomly selected. Statistical calculations were carried out based on the Jaccard similarity coefficient and UPGMA-based grouping in the NTSYS software (version 2.02). The amplitude of the multiplied bands varied between 100 and 3000 of alkaline pair. The polymorphism of all primers was 82.33% within the similarity limit. The Cophenetic coefficient for the similarity matrix and the resulting curve was obtained to be r=0.75. Genotypes 4 and 18 with a similarity coefficient of 0.91% demonstrated the highest similarity while genotypes 21 and 15 with a similarity coefficient of 0.63% showed the lowest similarity. Of the primers in use, the OPB-18 primer produced 12 bands (the highest number of bands) and the OPA-09 primer produced 5 bands (the lowest number of bands). Cluster analysis also confirmed the results obtained from the profiles produced in the genetic differentiation of cultivars under study as well as the correlations resulting from the Jaccard similarity coefficient. Finally, genotypes were categorized into 13 groups based on the results and resulting dendrogram. Results of the cluster analysis performed using the Jaccard similarity coefficient revealed the genetic diversity among genotypes that emphasize on efficiency of selection in sugar beet genotypes.

Key words: Beta vulgaris, RAPD, genetic analysis, cluster analysis, UPGMA

Corresponding author: A.R. Golparvar, Department of Agronomy and plant Breeding, College of Agriculture, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran. E-mail: dragolparvar@gmail.com. Phone:+989131816002. Fax:+9835354060

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Published in „GENETIKA“ Vol. 46, No.3(2014), pp. 985-1001

Serbia

UDC 575

DOI: 10.2298/GENSR1403985S

Original scientific paper

MOLECULAR EVALUATIONS OF THIRTY ONE CLONES OF POPLAR BASED

ON RAPD AND SSR MOLECULAR MARKERS

M.K.SINGH¹, N.B.SINGH¹, S.THAKUR¹, P.K.NAIK²

¹Department of Tree Improvement and Genetic Resources, College of forestry, Dr. Y. S. Parmar University of Horticulture and Forestry, Nauni, Solan, India

²Department of Biotechnology and Bioinformatics, Jaypee University of Information Technology, Waknaghat, Distt.- Solan, Himachal Pradesh, India

Abstract

Singh M.K., N.B.Singh, S.Thakur, and P.K.Naik (2014): Molecular evaluations of thirty one clones of poplar based on RAPD and SSR molecular markers- Genetika, Vol 46, No. 3, 985-1001

Poplar is an important tree species valued all over the world for its wood importance. Despite limited knowledge of the levels of genetic diversity and relatedness, their cultivation as a source of plywood is widespread. In order to facilitate reasoned scientific decisions on its management and conservation and prepare for selective breeding programme, genetic analysis of 31 genotypes was performed using RAPD and SSR molecular markers. Twenty six RAPD primers and 14 SSR primers amplified a total of 236 and 85 scoreable bands of which 86.44% and 86.02% were polymorphic. The mean coefficient of gene differentiation (Gst) was 0.388 and 0.341 indicating that 61.2% and 65.9% of the genetic variation resided within the populations. Analysis of molecular variance (AMOVA) indicated that majority of genetic variation (94.6% using RAPD and 89% using SSR) occurred among genotypes, while the variation between the three groups (categorized as tall, medium and small plants height) was 5.4% (using RAPD and 11% (using SSR). The dendrogram obtained from NJ and STRUCTURE analysis revealed splitting of genotypes into four clusters with clear distinction between short, medium and tall height genotypes, indicated that genetic differentiations measure with respect to RAPD and SSR. However, both the markers were equally useful in providing some understanding about the genetic relationship of different genotypes of poplar that are important in the conservation and exploitation of poplar genetic resources.

Key words: : genetic diversity, poplar clones, RAPD, SSR, RAPD+SSR

Corresponding author: M.K.Singh,Department of Tree Improvement and Genetic Resources, College of forestry, Dr. Y. S. Parmar University of Horticulture and Forestry, Nauni, Solan, India, mintusingh78@gmail.com

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Published in „GENETIKA“ Vol. 46, No.3(2014), pp. 1003-1012

Serbia

UDC 575

DOI: 10.2298/GENSR1403003B

Original scientific paper

INDUCTION OF MICRONUCLEI BY CADMIUM CHLORIDE IN AO RATS DEPENDS ON AGE AND SEX

Slavica POPOVIĆ BUBUJUK^1,2, Adrian MUSTER³; Ninoslav DJELIĆ⁴; Dragan KATARANOVSKI⁵, Marko ANDJELKOVIĆ⁶

¹Elementary school “Veselin Masleša”, Belgrade, Serbia

²Faculty of Biofarming, Megatrend University, Bačka Topola, Serbia

³Faculty of mechanical engineering, University of Belgrade, Belgrade, Serbia

⁴Faculty of Veterinary Medicine, University of Belgrade, Belgrade, Serbia

⁵Faculty of Biology, University of Belgrade, Belgrade, Serbia

⁶ Serbian Academy of Science and Arts, Belgrade, Serbia

Abstract

Popović Bubujuk S., A. Muster; N. Djelić; D.Kataranovski, M. Andjelković (2014): Induction of micronuclei by cadmium chloride in AO rats depends on age and sex- Genetika, Vol 46, No. 3, 1003-1012

Cadmium (Cd) is one of the most toxic industrial and metal in the environment which may cause severe genotoxic effects. The aim of the work was an evaluation of genotoxic effects of CdCl₂ in genetically pure Albino Oxford (AO) rats, depending on sex, age and dosage. Experimental animals were treated intraperitoneally with three different concentrations of CdCl₂: 0.5, 1, and 2 mg/kg of CdCl₂, while the control animals received equal volume of sterile phosphate buffered saline. The individuals of both sexes were treated at the age of 3, 6 and 12 month. Frequency of micronuclei formation was evaluated in polychromatic erythrocytes (PCEs), 24h hours after the treatment. The results showed that CdCl₂ caused a concentration-dependent increase of micronucleus frequency. The most significant differences were found between ages of 3/12 and 6/12 months at 0.5 and 1.0 mg CdCl₂ concentrations. Namely, 3 month old males had higher frequency of MNi in comparison to 12 month old males, whereas in females it was the opposite. Likewise, 6 months old males exbibited greater sensistivity to CdCl₂ in comparison to 12 month old rats, and in the females it was the opposite. Sex differences were further confirmed as slightly stronger genotoxic effects in 12 months old females treated with 0.5 and 1 mg/kg of CdCl₂. Therefore, the genotoxic effects of cadmium in AO rats depend on concentration, age and sex.

Key words: cadmium, micronuclei, Albino Oxford rats

Corresponding author: Ninoslav Djelić,Department of Biology,Faculty of Veterinary Medicine University of Belgrade,Oslobodjenja Blvd. 18,11000 Belgrade, Serbia,Phone: +381 11 265 88 94, Fax: +381 11 268 59 36,E-mail: ndjelic@vet.bg.ac.rs

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Published in „GENETIKA“ Vol. 46, No.3(2014), pp. 1013-1026

Serbia

UDC 575

DOI: 10.2298/GENSR1403013V

Original scientific paper

A MICROARRAY GENE EXPRESSION DATA CLASSIFICATION USING HYBRID BACK PROPAGATION NEURAL NETWORK

M.VIMALADEVI^1*, .B.KALAAVATHI²

¹Department of Computer Science and Engineering,

Hindustan Institute of Technology, Coimbatore, Tamilnadu, India.

²Department of Computer Science and Engineering,

K.S.R. Institute for Engineering and Technology, Tiruchengode, Tamilnadu, India

Abstract

Vimaladev M, .B. Kalaavathi (2014): A microarray gene expression data classification using hybrid back propagation neural network- Genetika, Vol 46, No. 3, 1013-1026

Classification of cancer establishes appropriate treatment and helps to decide the diagnosis. Cancer expands progressively from an alteration in a cell's genetic structure. This change (mutation) results in cells with uncontrolled growth patterns. In cancer classification, the approach, Back propagation is sufficient and also it is a universal technique of training artificial neural networks. It is also called supervised learning method. It needs many dataset for input and output for making up the training set. The back propagation method may execute the function of collaborate multiple parties. In existing method, collaborative learning is limited and it considers only two parties. The proposed collaborative function can perform well and problems can be solved by utilizing the power of cloud computing. This technical note applies hybrid models of Back Propagation Neural networks (BPN) and fast Genetic Algorithms (GA) to estimate the feature selection in gene expression data. The proposed research work examines many feature selection algorithms which are “fragile”; that is, the superiority of their results varies broadly over data sets. By this research, it is suggested that this is due to higher-order interactions between features causing restricted minima in search space in which the algorithm becomes attentive. GAs may escape from such minima by chance, because works are highly stochastic. A neural net classifier with a genetic algorithm, using the GA to select features for classification by the neural net and incorporating the net as part of the objective function of the GA.

Key words: Gene Expression Data, Back Propagation Neural Network, Genetic Algorithm, Feature Selection.

Corresponding author: M.Vimaladevi, Assistant Professor, Department of Computer Science and Engineering, Hindustan Institute of Technology, Coimbatore Tamilnadu, India. E-Mail: vimaladevmm@gmail.com

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Published in „GENETIKA“ Vol. 46, No.3(2014), pp. 1027-1036

Serbia

UDC 575

DOI: 10.2298/GENSR1403027G

Original scientific paper

YIELD AND NUTRITIONAL QUALITY OF GREENHOUSE LETTUCE

(Lactuca sativa L.) AS AFFECTED BY GENOTYPE AND PRODUCTION METHODS

Aleksandra GOVEDARICA-LUČIĆ, Mirjana MOJEVIĆ, Goran PERKOVIĆ, Branka GOVEDARICA

University in East Sarajevo, Faculty of Agriculture

East Sarajevo, Bosnia and Herzegovina

Abstract

Govedarica-Lučić A., M. Mojević, G. Perković, and B. Govedarica (2014): Yield and nutritional quality of greenhouse lettuce (Lactuca sativa L.) as affected by genotype and production methods- Genetika, Vol 46, No. 3, 1027-1036

Greenhouse experiments were conducted in winter growing seasons in order to evaluate the effects of genotype and production methods on yield and nutritional quality of lettuce (Lactuca sativa L.). A three-year (2009-2011) study was conducted by randomized block system in a greenhouse without additional heating. The trial included three genotypes of lettuce (Archimedes RZ, Santoro RZ, Kibou RZ). Each row with these genotypes was exposed to the following variants of covering: control-planting on bare soil, mulching before sowing with PE-black foil, agro textile-covering plants after planting with agro textile (17 g), a combination of mulching + agro textile. Throughout of all the three years of the trial, it was continuously evidenced that the genotype “Santoro RZ” had the biggest heads and the highest yield (15.33 kg 10 m^-2), which leads to conclusion that the yield of lettuce is a genotype characteristics. Moreover, the nutritional value (ascorbic acid concentration) has shown that, depending on the method of production, in average, the combination of mulching + agro textile (26.77 mg 100 g^-1) had the highest content while the control variant had significantly lower vitamin C content (21.10 mg 100 g^-1). The three-year researches have shown that the production method and genotype significantly affect the nitrate content. An average nitrate content was 2196.33 mg kg^-1 on the control variant, and 2526.24 mg kg^-1 on agro textile. Leafy lettuce of genotyp „Kibou RZ“ had lower nitrate content (2176.85 mg kg^-1) compared to „Archimedes RZ“ (2843.05 mg kg^-1) and „Santoro RZ“ (2221.37 mg kg^-1). However, nitrate concentration in all treatments remained within the European Union’s permissible levels.

Key words: lettuca,genotype, nitrate content, vitamine C.

Corresponding author: Aleksandra Govedarica-Lučić,University of East Sarajevo, Faculty of Agriculture, sandraklepic@yahoo.com;+38757342-701

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Published in „GENETIKA“ Vol. 46, No.3(2014), pp. 1037-1046

Serbia

UDC 575

DOI: 10.2298/GENSR1403037I

Original scientific paper

GENETIC ANALYSIS OF CHLOROPHYLL CONTENT IN MAIZE BY MIXED MAJOR AND POLYGENE MODELS

Muhammad IRFAN^#, Jia-xing SUN^#, Yanbin LIU, Xue LI, Shuang YANG*

Biotechnology Research Center, Shenyang Academy of Agricultural Science, Liaoning China

Abstract

Irfan M., J. Sun^#, Y. Liu, X. Li, and S. Yang (2014): Genetic analysis of chlorophyll content in maize by mixed major and polygene models- Genetika, Vol 46, No. 3, 1037-1046

Chlorophyll is an important factor which also affects the yield in maize. In this study, genetic analysis of chlorophyll content was conducted by joint segregation analysis of four generations P₁, P₂, F₁ and F_2:4 from the cross Shen3336ｘShen3265 using the mixed major genes and polygenes inheritance models. Genetics of chlorophyll revealed that chlorophyll “a” was controlled by two main gene having additive-dominance-epistasis effects. The heritability of these genes were 56.3%. Chlorophyll “b” was controlled by two pairs of codominant major gene plus additive-dominance polygene. The heritability of these major genes and polygenes were 1.12% and 93.26% respectively. Chlorophyll “a+b” was controlled by two pairs of additive-dominance- epistatic major genes plus additive- dominance polygene having heritability of 56.2% and 5.2% respectively.

Key words: Chlorophyll, SSR, QTL, Maize

Corresponding author: Jia-xing Sun, Biotechnology Research Center, Shenyang Academy of Agricultural Science, Liaoning China, yangshuangsaas@gmail.com

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Published in „GENETIKA“ Vol. 46, No.3(2014), pp. 1047-1063

Serbia

UDC 575:633.11

DOI: 10.2298/GENSR1403047K

Original scientific paper

MICROSATELLITES IN THE ANALYSIS OF WHEAT GENETIC DIVERSITY

Ankica KONDIĆ-ŠPIKA, Milica NIČIĆ, Ljiljana BRBAKLIĆ, Dragana TRKULJA,

Dragana MILADINOVIĆ, Siniša JOCIĆ, Milka BRDAR, Nikola HRISTOV

Institute of Field and Vegetable Crops, Novi Sad, Serbia

Abstract

Kondić-ŠpikaA., M. Ničić, Lj.Brbaklić, D. Trkulja, D. Miladinović, S. Jocić, M. Brdar, N. Hristov (2014): Microsatellites in the analysis of wheat genetic diversity.- Genetika, Vol 46, No. 3, 1047-1063

Microsatellite markers (SSR) were used to study wheat genetic diversity. A set of 87 wheat genotypes was analysed with four SSR markers. Primers used for the amplification of adequate microsatellite loci (Xgwm) are according to röder et al. (2002). Results were obtained using Applied Biosystems 3130 genetic analyser. Total of 28 alleles were determined, i.e. average of 7 alleles per marker. Number of alleles for individual markers ranged from six (Xgwm3) to eight (Xgwm18). The presence of two null alleles for Xgwm18 and Xgwm155 was found. There were five rare alleles (frequency <2%). Polymorphism information content (PIC) values ranged from 0.52 for Xgwm408 to 0.80 for Xgwm18. Mean PIC value was 0.69 for all markers, which signifies a high level of the detected polymorphism. According to the data collected through the analysis of four markers, most genotypes can be grouped in clusters. The results show usefulness of microsatellite markers in detecting polymorphism, identifying genotypes and assessing genetic diversity.

Key words: genetic variability, SSR markers,.Triticum aestivum L.

Corresponding author: Ankica Kondić-Špika, Institute of Field and Vegetable Crops, M. Gorkog 30, 21000 Novi Sad, Serbia, e-mail: ankica.spika@nsseme.com, Phone: +381 21 4898 224, Fax: +381 21 4898 222.

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