GENETIKA, Vol. 41, No. 3 (2009)
PROMEMORIA Academicia SLAVKO BOROJEVIĆ (1999 – 2009) [Full text]
Milomirka MADIĆ, Desimir KNEŽEVIĆ, Aleksandar PAUNOVIĆ, and Veselinka ZEČEVIĆ
INHERITANCE OF STEM HEIGHT AND SECOND-INTERNODE LENGTH IN BARLEY HYBRIDS [Abstract] [Full text]
Domagoj ŠIMIĆ, Tatjana LEDENČAN, Antun JAMBROVIĆ, Zvonimir ZDUNIĆ, Josip BRKIĆ, Andrija BRKIĆ, Snežana MLADENOVIĆ DRINIĆ, and Ivan BRKIĆ
SNP and SSR MARKER ANALYSIS AND MAPPING OF A MAIZE POPULATION [Abstract] [Full text]
Veselinka ZEČEVIĆ, Desimir KNEŽEVIĆ, Jelena BOŠKOVIĆ and Milomirka MADIĆ
EFFECT OF GENOTYPE AND ENVIRONMENT ON WHEAT QUALITY [Abstract]
[Full text]
Mihailo NIKOLIĆ, Aleksandar RADOVIĆ, Milica FOTIRIĆ, Jasminka MILIVOJEVIĆ and Dragan NIKOLIĆ
POMOLOGICAL PROPERTIES OF PROMISING RASPBERRY SEEDLINGS WITH YELLOW FRUIT [Abstract] [Full text]
Jovanka ATLAGIĆ, Ana MARJANOVIĆ-JEROMELA, Radovan MARINKOVIĆ, and Sreten TERZIĆ
POLLEN GRAIN TRAITS OF OIL SPECIES FROM THE NOVI SAD COLLECTION [Abstract] [Full text]
Dragan PEROVIĆ, Dragica ZORIĆ, Milivoje MILOVANOVIĆ, Slaven PRODANOVIĆ, Yueming YAN, Snežana JANKOVIĆ and Gordana SURLAN-MOMIROVIĆ
HORDEIN GENE DOSE EFFECTS IN TRIPLOID ENDOSPERM OF BARLEY (Hordeum vulgare L.) [Abstract] [Full text]
Sreten TERZIĆ and Jovanka ATLAGIĆ
NITROGEN AND SUGAR CONTENT VARIABILITY IN TUBERS OF JERUSALEM ARTICHOKE (HELIANTHUS TUBEROSUS ) [Abstract] [Full text]
Slađana ŽILIĆ, Vesna HADŽI-TAŠKOVIĆ ŠUKALOVIĆ, Mirjana SREBRIĆ, Dejan DODIG, Milan MAKSIMOVIĆ, Snežana MLADENOVIĆ DRINIĆ and Miloš CREVAR
CHEMICAL COMPOSITIONS AS QUALITY PARAMETERS OF ZP SOYBEAN AND WHEAT GENOTYPES [Abstract] [Full text]
Vasilije ISAJEV, Vladan IVETIĆ, Aleksandar LUČIĆ, and Ljubinko RAKONJAC
GENE POOL CONSERVATION AND TREE IMPROVEMENT IN SERBIA [Abstract] [Full text]
Milan KULIĆ,Nevenka ALEKSIĆ,Zoran STANIMIROVIĆ,Siniša RISTIĆ,and Snežana MEDENICA
EXAMINATION OF GENOTOXIC EFFECTS OF FUMAGILLIN IN VIVO [Abstract] [Full text]
Amarela TERZIC-VIDOJEVIC, Jelena LOZO, and Ljubisa TOPISIROVIC
DOMINANT LACTIC ACID BACTERIA IN ARTISANAL PIROT CHEESES OF DIFFERENT RIPENING PERIOD [Abstract] [Full text]
Jelena BOŠKOVIĆ and Momčilo BOŠKOVIĆ
DURABLE RESISTANCE TO PUCCINIA TRITICINA BY ACCUMULATION OF RESISTANCE GENES [Abstract] [Full text]
Published in „GENETIKA“ Vol. 41, No. 3 (2009), pp. 229-236
© 2009 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC: 575:633.16
DOI: 10.2298/GENSR0903229M
Original scientific paper
INHERITANCE OF STEM HEIGHT AND SECOND-INTERNODE LENGTH IN BARLEY HYBRIDS
Milomirka MADIĆ1, Desimir KNEŽEVIĆ2, Aleksandar PAUNOVIĆ1, and Veselinka ZEČEVIĆ3
1Faculty of Agronomy Čačak
2Faculty of Agriculture, Zubin Potok
3Faculty of Biofarming, Bačka Topola
Abstract
Madic M., D. Knežević, A. Paunović and V. Zečević (2009): Inheritance of stem height and second-internode length in barley hybrids - Genetika, Vol.41, No 3, 229-236
Stem height and second (basal) internode are highly important components of lodging resistance in barley. A diallel crossing of five divergent barley genotypes (KG-1/90, NS-293, Jagodinac, KG-15 and KG-10/90) was carried out in order to examine the modes of inheritance of stem height and second-internode length. The dominance of a parent with a higher stem was the prevailing mode of stem height inheritance in most of the combinations in F1 and F2 generations, the degree of dominance ranging from partial to superdominance. Partial dominance mostly occurred in the inheritance of second-internode length in most crossing combinations.
An analysis of genetic variance components showed that the dominant gene effect was the most contributing factor to the inheritance of stem height. Additive effect genes prevailed in the inheritance of second-internode length in the F1 generation. Both additive and dominant gene effects played an important role in the F2 generatio
Key words: barley, inheritance, second-internode length, stem height
Corresponding author: Corresponding author: Milomirka Madic, Faculty of Agronomy, Cara Dusana 34, 3200 Cacak, e-mail: mmadic@tfc.kg.ac.rs
Return to content
Published in „GENETIKA“ Vol. 41, No. 3 (2009), pp. 237-246
© 2009 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:633:15
DOI: 10.2298/GENSR0903237S
Original scientific paper
SNP and SSR MARKER ANALYSIS AND MAPPING OF A MAIZE POPULATION
Domagoj ŠIMIĆ1, Tatjana LEDENČAN1, Antun JAMBROVIĆ1, Zvonimir ZDUNIĆ1, Josip BRKIĆ1, Andrija BRKIĆ1, Snežana MLADENOVIĆ DRINIĆ2, and Ivan BRKIĆ1
1 Agricultural Institute Osijek, Osijek, Croatia
2 Maize Research Institute, Belgrade, Serbia
Abstract
Šimić D., T. Ledenčan, A. Jambrović, Z .Zdunić, J.Brkić, A.Brkić, S.Mladenović Drinić and I. Brkić (2009): SNP and SSR marker analysis and mapping of a maize population. - Genetika, Vol. 41, No. 3, 237-246.
Although highly polymorphic SSRs are currently the marker of choice worldwide in maize breeding, single nucleotide polymorphisms (SNPs) as a newer marker system are recently used more extensively. The objective of this study was investigate the utility of SSR and SNP markers for mapping of a maize population adapted to conditions of Southeast Europe. Total of 294 F2:3 lines derived from a biparental mapping population were genotyped using 121 polymorphic SNP and SSR markers. The SNP markers were analyzed using the SNPlex technology. 56 of the 142 tested SNPs (39%) were polymorphic between the parents of the mapping population and were successfully mapped. The remaining markers were either not functional (5 = 3.5%) or not polymorphic (81 = 57%). No mapped SNP marker showed more than 10% missing data. On average, the level of missing data for SNPs (1.5%) was considerably lower than that for SSRs (3.4%). For the mapping procedure, the SNP data were combined SSR data. A comparison of the mapping data with the publicly available mapping data on SSR markers and the proprietary mapping data indicates that the map is of good quality and that the map position of almost all markers agrees with their published map position. Thus, information obtained from both marker systems is utilizable for further QTL analysis.
Key words: maize population, mapping, SNP markers, SSR makers
Corresponding author: Domagoj Šimić, Department of Maize Breeding and Genetics, Agricultural Institute Osijek, Južno predgrađe 17, 31000 Osijek, Croatia, e-mail: domagoj.simic@poljinos.hr
Published in „GENETIKA“ Vol. 41, No. 3 (2009), pp. 247-253
© 2009 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:633.11
DOI: 10.2298/GENSR0903247Z
Original scientific paper
EFFECT OF GENOTYPE AND ENVIRONMENT ON WHEAT QUALITY
Veselinka ZEČEVIĆ1, Desimir KNEŽEVIĆ2, Jelena BOŠKOVIĆ1
and Milomirka MADIĆ3
1Megatrend University Belgrade, Faculty of Biofarming Bačka Topola, Serbia,
2Faculty of Agriculture, Zubin Potok, Serbia
3 Faculty of Agronomy, Čačak, Serbia
Abstract
Zečević V., D. Knežević, J. Bošković and M. Madić (2009): Effect of genotype and environment on wheat quality.- Genetika, Vol 41, No. 3, 247-253.
Five winter wheat cultivars created in Small Grains Research Centre of Kragujevac (Ana Morava, Toplica, Vizija, Takovčanka and Lazarica) were grown at the macro field trial in three locations (Kragujevac, Sombor and Bačka Topola) during three years (2004-2006). Influence of genetic and agro-ecological conditions of locations on wheat quality components (sedimentation value and wet gluten content) was investigated. The analysis of variance suggested there were highly significant differences among genotypes (G), investigated years (Y) and locations (L) for sedimentation value and wet gluten content. Apart from individual influence of the factors, their interactions (G x Y, G x L, Y x L, G x Y x L) were also high significant for both investigated traits. In average the highest sedimentation value (40.6 ml) and wet gluten content (31.85 %) established at Bačka Topola locality. The highest value of all investigated cultivars and localities established at cultivar Vizija (45.3 ml) in Bačka Topola, while the lowest at Lazarica (31.7 ml) in Sombor. The highest wet gluten content was measured at Bačka Topola locality by cultivar Toplica (38.53 %). In this investigation Bačka Topola locality was favourable for both investigated quality traits.
Key words: genotype-environment interaction, gluten content, quality, sedimentation value, wheat
Corresponding author: Prof. dr Veselinka Zečević, Megatrend University Belgrade, Faculty of Biofarming, Maršala Tita 39, Bačka Topola, Serbia, phone + 381 24 718515, e-mail: joca@kg.ac.rs
Published in „GENETIKA“ Vol. 41, No.3 (2009), pp. 255-262
© 2009 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC:575:634.711
DOI: 10.2298/GENSR0903255N
Original scientific paper
Faculty of Agriculture, Belgrade-Zemun, Serbia
Abstract
Key words: Meeker, raspberry, seedling, selection, yellow fruit.
Corresponding author: Milica Fotirić, Faculty of Agriculture, Nemanjina 6, 11080 Belgrade-Zemun, Serbia, phone: +381 11 2615315/457 e-mail: fotiric@agrif.bg.ac.rs
Published in „GENETIKA“ Vol. 41, No. 3 (2009), pp. 263-270
© 2009 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC:575:633.85
DOI: 10.2298/GENSR0903263A
Original scientific paper
Jovanka Atlagić, Ana Marjanović-Jeromela, Radovan Marinković, and Sreten Terzić
Institute of Field and Vegetable Crops, Novi Sad, Serbia
Abstract
Altagić J., A. Marijanović-Jernomela, R.Marinković, and S. Terzić (2009):Pollen grain traits of oil species from the Novi Sad collection - Genetika, Vol. 41, No. 3, 263-270.
The collection of oil species in Novi Sad contains 12 species represented with 1-4 cultivars or landraces. In the continuous work on this collection in the sense of breeding of some of those species and their usage as a source of «desirable genes» we analyzed pollen grain morphology (shape and size), as well as pollen viability. To determine mentioned pollen traits we used Axiovert 40C microscope together with a software package (AxioVision LE; Rel.4.3.) for measurement of pollen length and width. Pollen viability was determined using a staining method (alexander, 1969). The results showed that species differ by pollen grain shape (round, egg-shaped, triangular and rod) as well as by shape of exine (thick and spiky, thick to thin). In some species there was a specific number of apertures present (1-11). The size of viable pollen grains ranged from 29,10/12,58µ (coriander) to 176,63/169,94µ (oil gourd), while non-viable pollen grains were always smaller (27,27/10,97µ to 119,62/100,86µ) at the same plant species. Pollen viability of most species was around 80%. Lowest pollen viability was found in white flax (56,98%), and the highest in oil pumpkin (91,43%).
Key words: oil species, pollen grain shape and size, pollen viability.
Corresponding author: dr Jovanka Altagić, Institute for field and vegetable crops, Novi Sad, Serbia, e-mail: atlagic@ifvcns.ns.ac.rs
Published in „GENETIKA“ Vol. 41, No. 3 (2009), pp. 271-287
© 2009 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC: 575:633.16
DOI: 10.2298/GENSR0903271P
Original scientific paper
HORDEIN GENE DOSE EFFECTS IN TRIPLOID ENDOSPERM OF BARLEY (Hordeum vulgare L.)
Dragan PEROVIĆ1, Dragica ZORIĆ1, Milivoje MILOVANOVIĆ2, Slaven PRODANOVIĆ1, Yueming YAN3, Snežana JANKOVIĆ4 and Gordana SURLAN-MOMIROVIĆ1
1Faculty of Agriculture, Belgrade, Serbia
2 Center for small Grains, Kragujevac, Serbia
3 College of Life Science, Capital Normal University, Beijing, China
4Institute for Science application in Agriculture, Belgrade, Serbia
Abstract
Perović D., D. Zorić, M. Milovanović, S. Prodanović, Y. Yan, S. Janković and G. Šurlan Momirović (2009): Hordein gene dose effects in triploid endosperm of barley (Hordeum vulgare L.).- Genetika, Vol. 41, No. 3, 271-287.
The presence of two maternal chromosome sets in triploid barley endosperm allows the distinction of maternal and paternal hordein bands in an electrophoregram: the maternal bands are stronger due to the higher gene dose. In the F1 generation there are differences between reciprocal crosses and in the F2 generation all 16 classes that are theoretically possible for a pair of polymorphic loci can be distinguished. This full classification is rarely possible in genetic studies, and allows more accurate estimates of recombination rates. Two hordein gene clusters (Hor1 and Hor2, corresponding to hordein C and hordein B respectively) were analysed in hybrids obtained by crossing two winter barley cultivars Partizan and HWV-247. Hordein separation was performed by acid-polyacrylamide gel electrophoresis at pH 3.2 (A-PAGE). A set of most informative bands of B and C hordeins was selected in each cross by two criteria: (1) presence or absence of bands in the parents and (2) signal strength to allow doses scoring. The average genetic distance between Hor1 and Hor2 loci was 11 cM. Distances in male and female maps were not significantly different, suggesting a similar recombination rate in male and female meiosis.
Key words: A – PAGE,barley, hordeins, gene dosage effect
Corresponding author: dr Dragan Perović, present address: Julius Kuehn-Institute, Federal research centre for cultivated plants, Institute for resistance research and stress tolerance, Quedlinburg, Germany, e-mail: dragan.perovic@jki.bund.de
Return to content
Published in „GENETIKA“ Vol. 41, No. 3 (2009), pp. 289-295
© 2009 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:633.494
DOI: 10.2298/GENSR0903289T
Original scientific paper
NITROGEN AND SUGAR CONTENT VARIABILITY IN TUBERS OF JERUSALEM ARTICHOKE (HELIANTHUS TUBEROSUS )
Sreten TERZIĆ and Jovanka ATLAGIĆ
Institute of Field and Vegetable Crops, Novi Sad, Serbia
Abstract
Terzić S., and J. Altagić (2009): Nitrogen and sugar content variability in tubers of jerusalem artichoke (Helianthus tuberosus ). - Genetika, Vol. 41, No.3, 289-295.
Several nutritive values for tubers of 114 Jerusalem artichoke (Helianthus tuberosus) populations were evaluated during 2006. The used material is a part of wild sunflower species collection at the Institute of field and vegetable crops and it is situated in Rimski Šančevi, Novi Sad. The samples were analyzed as fresh tubers on ”Venema” automatic laboratory for alpha amino nitrogen, sodium and potassium content. Total sugar content was determined as the brix value on a refractometer. Total nitrogen was determined by the Kjeldahl method on dried samples.
Significant variability was found for all analyzed traits. Total nitrogen varied from 0,695 to 2,179% dry weight (mean 1,23%), alpha amino nitrogen content 0,012 to 0,118% fresh weight (m. 0,07%), potassium 0,231 – 0,452% fresh weight (m. 0,403%) and sodium 0,0003 – 0,0143% fresh weight (m. 0,007%). Total sugar content varied from 13,69 – 22,94% fresh weight (m. 19,14%).
Alpha amino nitrogen is an essential nutrient for animals so that it’s presence in tubers of Jerusalem artichoke as food is positive. The protein content is similar to the one in potato and as such satisfactory for nutrition. The K/Na ratio is high which is useful, because an increased content of potassium in food can positively affect the reduction of Na/K ratio and lower systolic blood pressure by a significant amount in adults with mild hypertension. Inulin makes up to 80% of the total sugar content in the tubers of Jerusalem artichoke, and as a dietary fiber and a fructose polymer it positively influences digestion and sugar blood levels.
The obtained results suggest that selection of cultivars and populations with mnappropriate nutritive values is possible. Further research is needed to estimate the share of genetic in total variability and to determine whether the selection for new cultivars is justified.
Key words: α-amino nitrogen, Helianthus tuberosus, sunflower, total sugar, variability.
Corresponding author: mr Sreten Terzić, Institute of Field and Vegetable Crops, Novi Sad. terzic@ifvcns.ns.ac.rs
Published in „GENETIKA“ Vol. 41, No.3(2009), pp. 297-308
© 2009 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC: 575:633
DOI:10.2298/GENSR0903297Z
Original scientific paper
CHEMICAL COMPOSITIONS AS QUALITY PARAMETERS OF ZP SOYBEAN AND WHEAT GENOTYPES
Slađana ŽILIĆ1, Vesna HADŽI-TAŠKOVIĆ ŠUKALOVIĆ2, Mirjana SREBRIĆ1, Dejan DODIG1, Milan MAKSIMOVIĆ3, Snežana MLADENOVIĆ DRINIĆ1 and Miloš CREVAR1
1Maize Research Institute, Zemun Polje, Serbia
2Institute for Multidisciplinary Research, Belgrade, Serbia
3Military Medical Academy, Institute of Hygiene, Belgrade, Serbia
Abstract
Žilić S., V. HadžiTašković Šukalović, M. Srebrić, D. Dodig, M. Maksimović, S. Mladenović Drinić, and M. Crevar (2009): Chemical compositions as quality parameters of ZP soybean and wheat genotypes.– Genetika, Vol. 41, No. 3, 297-308.
This research is focused on the analysis of chemical characteristics of ZP soybean and wheat genotypes, as well as, on nutritional differences between this two complementary plant species. The experimental material consisted of two bread (ZP 96/I and ZP 87/Ip), two durum (ZP 34/I ZP and ZP DSP/01-66M) wheat genotypes and four soybean varieties (Nena, Lidija, Lana and Bosa) of different genetic background. All ZP soybean genotypes, except the Lana, had over 40% of total proteins by dry matter. Lana and Lidija, variety of recent creation, developed as a result of selection for specific traits, had high oil content. Wheat genotypes had much a lower content of ash, oil, total and water soluble proteins than soybean cultivars. The highest oil, total and water soluble proteins content was detected in grain of durum genotype ZP DSP/01-66M. Lignin content varies much more among soybean than among the wheat genotypes. Generally, contents of total phenolics, carotenes and tocopherol were more abundant in ZP soybean than bread and durum wheat genotypes.
Key words: antrinutritional components, protein, oil, ZP genotypes
Corresponding author: Slađana Žilić, Maize Research Institute, Zemun Polje, Department of Technology; Slobodana Bajića 1, 11185 Zemun Polje, Belgrade-Zemun, Serbia, tel. +381 37 56 704; fax +381 37 54 994, e-mail: szilic@mrizp.rs
Published in „GENETIKA“ Vol. 41, No. 3 (2009), pp. 309-327
© 2009 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC 575:630
DOI: 10.2298/GENSR0903309I
Original scientific paper
GENE POOL CONSERVATION AND TREE IMPROVEMENT in SERBIA
Vasilije Isajev1, Vladan IVETIĆ1, Aleksandar Lučić2, and Ljubinko RAKONJAC2
1Faculty of Forestry, Belgrade
2Forest research Institute, Belgrade
Abstract
Isajev V., V. Ivetić, A. Lučić, and Lj. Rakonjac (2009): Gene pool conservation and tree improvement in Serbia.- Genetika, Vol. 41, No. 3, 309-327.
This paper presents the concepts applied in the gene pool conservation and tree improvement in Serbia. Gene pool conservation of tree species in Serbia includes a series of activities aiming at the sustainability and protection of genetic and species variability. This implies the investigation of genetic resources and their identification through the research of the genetic structure and the breeding system of individual species. Paper also includes the study of intra- and inter-population variability in experiments - provenance tests, progeny tests, half- and full-sib lines, etc. The increased use of the genetic potential in tree improvement in Serbia should be intensified by the following activities: improvement of production of normal forest seed, application of the concept of new selections directed primarily to the improvement of only one character, because in that case the result would be certain, establishment and management of seed orchards as specialised plantations for long-term production of genetically good-quality forest seeds, and the shortening of the improvement process by introducing new techniques and methods (molecular markers, somaclonal variation, genetic engineering, protoplast fusion, micropropagation, etc.).
Key words: conservation, gene pool, tree improvement
Corresponding author: prof dr Vasilije Isajev, Faculty of Forestry, Kneza Višeslava 1, 11000 Belgrade, Serbia
Published in „GENETIKA“ Vol. 41, No. 3 (2009), pp. 329-338
© 2009 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC. 575
DOI: 10.2298/GENSR0903329K
Original scientific paper
GENETIC VARIATION OF PHYTATE AND IONORGANIC PHOSPHORUS IN MAIZE EXAMINATION OF GENOTOXIC EFFECTS OF FUMAGILLIN IN VIVO
Milan KULIĆ¹, Nevenka ALEKSIĆ2, Zoran STANIMIROVIĆ², Siniša RISTIĆ¹, and Snežana MEDENICA¹
¹ Faculty of Medicine, University of Sarajevo, East Sarajevo, Bosnia and Herzegovina
² Faculty of Veterinary Medicine, University of Belgrade, Belgrade, Serbia
Abstract
Kulic M., N. Aleksić, Z. Stanimirović, S. Ristić, and S. Medenica (2009): Examination of genotoxic effects of fumagillin in vivo.- Genetika, Vol. 41, No. 3, 329-338.
Fumagillin is an antibiotic derived from the fungus Aspergillus fumigatus. It has been used successfully for the treatment of intestinal microsporidiosis in HIV-positive humans, as well as in those suffering from intestinal amebiasis and microsporidial keratoconjunctivitis. In veterinary medicine it is approved for the treatment of microsporidiosis in bees and fish. In this research fumagillin was tested for the ability to provoke chromosomal aberrations in mouse bone marrow cells. BALB/c mice were administered fumagillin by gastric probe in doses of 5, 10 and 20 mg/kg b.w. Water-sugary syrup was the negative and cyclophosphamide (15 mg/kg b.w.) the positive control. Significantly increased frequencies (p<0.01 or p<0.001) of numerical chromosomal aberrations (aneuploidies and poliploidies) was observed both in the medium (10 mg/kg b.w.) and the highest (20 mg/kg b.w.) dose of fumagillin. Structural chromosomal aberrations (gaps, breaks and insertions) were noticeably more frequent in comparison to negative control only in the highest experimental dose of dycikloheksilamine. These results clearly showed that fumagillin in concetrations 10 and 20 mg/kg b.w. had a genotoxic potential in vivo.
Key words: chromosomal aberrations, genotoxicity, fumagillin (dicyclohexylamine).
Corresponding author: Dr Zoran Stanimirović, Department of Biology, Faculty of Veterinary Medicine, University of Belgrade,11000 Belgrade, Serbia, Blvd. oslobodjenja 18, e-mail: zoran@vet.bg.ac.rs
Published in „GENETIKA“ Vol. 41, No. 3(2009), pp. 339-352
© 2009 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC:575
DOI: 10.2298/GENSR0903341T
Original scientific paper
DOMINANT LACTIC ACID BACTERIA IN ARTISANAL PIROT CHEESES OF DIFFERENT RIPENING PERIOD
Amarela TERZIC-VIDOJEVIC, Jelena LOZO, and Ljubisa TOPISIROVIC
Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia
Abstract
Terzic-Vidojevic A., J. Lozo, and Lj. Topisirovic (2009):Dominant lactic acid bacteria in artisanal Pirot cheeses of different ripening period. – Genetika, Vol. 41, No. 3, 339-352.
In this study two raw cow’s milk cheeses of a different ripening period were examined. The cheeses were taken from a country household in the region of mountain Stara Planina and manufactured without adding of starter culture. A total 106 lactic acid bacteria (LAB) strains were isolated from both cheeses. They are tested by classical physiological tests as well as by API 50 CH tests. Proteolytic and antimicrobial activities were done too. Identification of LAB isolates was done by repetitive extragenic palindromic-polimerase chain reaction (rep-PCR) with (GTG)5 primer. The LAB isolates from cheese BGPT9 (four days old) belonged to the eight species of LAB (Lactobacillus plantarum, Lactobacillus paracasei subsp. paracasei, Lactobacillus delbrueckii, Lactobacillus brevis, Enterococcus faecium, Enterococcus faecalis, Enterococcus durans and Leuconostoc garlicum), while in the BGPT10 cheese (eight months old) only two species were present (Lactobacillus plantarum and Enterococcus faecium). Proteolytic activity showed 30 LAB from BGPT9 cheese, mainly enterococci. From BGPT10 cheese only one isolate (which belonged to the Lactobacillus plantarum species) possessed partial ability to hydrolyze β-casein. Seven enterococci from BGPT9 cheese and four enterococci from BGPT10 cheese produced antimicrobial compounds.
Key words: antimicrobial activity, non-starter lactic acid bacteria (NSLAB), Pirot cheese, rep-PCR
Corresponding author: Amarela Terzic Vidojevic, Institute of Molecular Genetics and Genetic Engineering, University of Belgrade,Vojvode Stepe 444a, P.O.Box 23, 11010 Belgrade, Serbia. Phone: +381 11 397 59 60; Fax: +381 11 397 58 08; e-mail: lab6@eunet.rs
Published in „GENETIKA“ Vol. 41, No. 3(2009), pp. 353-378
© 2009 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade-Zemun
Serbia
UDC:575
DOI: 10.2298/GENSR0903355B
Review paper
DURABLE RESISTANCE TO PUCCINIA TRITICINA BY ACCUMULATION OFRESISTANCE GENES
Abstract
Bošković J., and M. Bošković (2009): Durable resistance to Puccinia triticina by accumulation of resistance genes- Genetika, Vol. 41, No. 3, 353-378.
The individual use of single race-specific resistance genes with major phenotypic effects has rarely provided lasting resistance. However, breeding and combining or pyramiding of resistance genes into individual cultivars has had considerable success, particularly in situations in which the pathogen does not reproduce sexually, as in the case of wheat leaf rust pathogen. In European-Mediterranean region perfomed international investigations of wheat leaf rust proved that breeding of new lines of wheat resistant to Puccinia triticina Eriks. for differentiation of pathogen population, as well as for sources of durable resistance is necessary. Breeding of such resistant lines has proved necessary due to the unsatisfatory survey results of these regions on standard isogenic Lr lines. It has become clear that these regions needed new, more efficient differential resistance genes, as well as sources of resistance. In the beginning, after extensive screening tests of several International Rust Nurseries, 18 donors of resistance had been selected as crosses with recurrent parents’ varieties Princ and Starke. These hybrid lines had been comparatively tested with twenty six Lr single gene lines using twenty especially virulent cultures of P. triticina in order to check the presence of these known Lr genes in our hybrid lines. Considerable influence of recurrent parent to the number of resistant genes in used donors was demonstrated. On the other hand, considerable influence of the pathogen culture was established to the number of resistance genes in used donors. In order to enhance resistance and pyramiding genes in these hybrids, the most interesting selected eight lines have been crossed with only effective isogenic ones, containing the strong genes Lr9, Lr19 and Lr24. On the basis of different segregation rations of all crossing combinations it was proved that no one of resistant donors contained the applied strong resistant genes. It means that our hybrid lines contained resistant genes from the donors, as well as three strong resistant genes Lr9, Lr19 and Lr24.
Key words: accumulation of resistance genes, hybrid lines, Puccinia triticina, resistant hybrid, wheat lines.