GENETIKA, Vol. 40, No.1 (2008)
Gordan ZEC, Slavica Čolić, Dejan Marinković and Dragan Nikolić
VARIABILITY OF VINEYARD PEACH TREE CHARACTERISTICS [Abstract] [Full text]
Ksenija TAŠKI-AJDUKOVIĆ, Vuk ĐORĐEVIĆ, Miloš VIDIĆ, Milka VUJAKOVIĆ, Mirjana MILOSEVIĆand Jegor MILADINOVIĆ
THE MAIN SEED STORAGE PROTEINS AMONG HIGH-PROTEIN SOYBEAN GENOTYPES [Abstract] [Full text]
Lazar KOJIC and Dillyara AJGOZINA
BREEDING AND GENETIC PROPERTIES OF THE MAIZE VARIETY UZBEKSKA BELA [Abstract] [Full text]
Mirjana VASIĆ, Jelica GVOZDANOVIĆ-VARGA and Janko ČERVENSKI
DIVERGENCE IN THE DRY BEAN COLLECTION BY PRINCIPAL COMPONENT ANALYSIS (PCA) [Abstract] [Full text]
Biljana TOŠOVIĆ-MARIĆ, Borislav KOBILJSKI, Dragana OBREH and Ljiljana VAPA
EVALUATION OF WHEAT RHT GENES USING MOLECULAR MARKERS [Abstract] [Full text]
Dragana OBREHT, Borislav KOBILJSKI, Mihajla ĐAN and Ljiljana VAPA
MARKER ASSISTED SELECTION IN BMQ RELATED BREEDING PROGRAMS [Abstract] [Full text]
Adriane MENSSEN and Peter A. PETERSON
A NINE-BASE PAIR DELETION DISTINGUISHES TWO EN/SPM TRANSPOSON ALLELES IN MAIZE: THEIR GENETIC ACTIVITY AND MOLECULAR DESCRIPTION [Abstract] [Full text]
Snežana B.PAJOVIĆ
NUTRIGENOMICS [Abstract] [Full text]
Milka BRDAR, Ivana MAKSIMOVIĆ, Borislav KOBILJSKI, Tijana ŠKORIĆ ZEREMSKI and Marija KRALJEVIĆ BALALIĆ
TOLERANCE OF WHEAT SEEDLINGS TO EXCESS BORON [Abstract] [Full text]
Ksenija MARKOVIĆ, Ana NIKOLIĆ, Dragana IGNJATOVIĆ-MICIĆ, Violeta ANĐELKOVIĆ and Vesna LAZIĆ-JANČIĆ
s1 families as a source of beneficial alleles for breeding drought tolerant maize genotypes [Abstract] [Full text]
Published in „GENETIKA“ Vol. 40, No.1 (2008), pp. 1-7
© 2008 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade - Zemun
Serbia
UDC 575: 634.25
Original scientific paper
VARIABILITY OF VINEYARD PEACH TREE CHARACTERISTICS
Gordan ZEC1, Slavica Čolić1, Dejan Marinković1 and Dragan Nikolić2
1Institute PKB Agroekonomik, Padinska Skela, Serbia
2Faculty of Agriculture, Zemun, Serbia
Abstract
Zec G, S. Čolić, D. Marinkovic and D.Nikolic (2008): Variability of vineyard peach tree characteristics.– Genetika, Vol. 40, No. 1, 1-7.
Vineyard peach seedlings are the most important rootstock for peach in Serbia and abroad. High variability is a characteristic of the vineyard peach planting material that is used as rootstock in nursery production. Through work of many years, vineyard peach genotypes with qualitative traits were selected and collected. Seedlings that are progeny of the nine selected genotypes and resulted from self and open pollination were examined. The vineyard peach seedlings resulted from uncontrolled pollination and with different geographical origin served as reference. A goal of research was to get vineyard peach genotypes that would give more uniform generative progeny with qualitative traits. This paper presents the results of two-year research of morphological traits of more than 500 vineyard seedlings. Based on the results, positive genotypes were selected for further inbreeding. Further, the seedlings of the selection 6 have the lowest coefficients of variation for trunk thickness, tree height and number of branches, which points to the self-pollination as a good method for getting more uniform progeny.
Key words: vineyard peach, tree, characteristic, variability
Corresponding author: Gordan Zec, Institut PKB Agroekonomik, Industrijsko naselje b.b., 11213 Padinska Skela, Srbija tel: 011 8871 175, e-mail: zec2004@yahoo.com
Published in „GENETIKA“ Vol. 40, No.1 (2008), pp. 9-16
© 2008 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade - Zemun
Serbia
UDC 575:633.34
Original scientific paper
THE MAIN SEED STORAGE PROTEINS AMONG HIGH-PROTEIN SOYBEAN GENOTYPES
Ksenija TAŠKI-AJDUKOVIĆ 1, Vuk ĐORĐEVIĆ 2, Miloš VIDIĆ 2, Milka VUJAKOVIĆ 1, Mirjana MILOSEVIĆ 1and Jegor MILADINOVIĆ 2
1National Laboratory for Seed Testing, Novi Sad
2Institute for Field and Vegetable Crops, Novi Sad, Serbia
Abstract
Taški - Hajduković K., V. Đorđević, M. Vidić, M. Vujaković, M. Milošević and J. Miladinović (2008): The main seed storage proteins among high .protein soybean genotypes.– Genetika, Vol. 40, No. 1, 9-16.
It is known that the main components of the seed storage proteins contribute to the quality of soybean [Glycine max (L.) Merr.] food products. The objective of this study was to investigate content of the two of them [glycinin (11S) and β-conglycinin (7S) fractions] and their respective subunits on the new high-protein soybean genotypes from the Institute for Field and Vegetable Crops, Novi Sad, Serbia. Subunits were resolved by SDS-PAGE and gels were analyzed by scanning densitometry.
Out of 20 analyzed genotypes, the β’ and β subunits of β-conglycinin were significantly higher in all of the genotypes except KO531 and KO5431. The β subunit of β-conglycinin was significantly higher in genotypes KO535 KO5437, KO534, KO537, KO539, KO5439, KO532, KO5435, KO538, KO5438 and KO533. The acetic polypeptides of glycinin were significantly higher in genotypes KO5439, KO5437, KO5436, KO5438, KO5432, KO5435, KO5433 and KO5434. The basic polypeptides of glycinin were not significantly higher only in genotypes KO539, KO536, KO538, KO535 and KO533. In conclusion, it appears that among the new high-protein genotypes there are genotypes with different amount of subunits that should be bread in the future for a desired level of the protein components.
Key words: Glycine max, β-conglycinin, glycinin, high-proteins seeds, soybean, storage proteins
Corresponding author: Ksenija Taski-Ajdukovic, Maksima Gorkog 30, 21000 Novi Sad, Serbia Phone 021/4898 157,fax: 021/ 421 249, e-mail: ksenijat@ifvcns.ns.ac.yu
Published in „GENETIKA“ Vol. 40, No.1 (2008), pp. 17-21
© 2008 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade - Zemun
Serbia
UDC 575: 633.15
Original scientific paper
BREEDING AND GENETIC PROPERTIES OF THE MAIZE VARIETY
UZBEKSKA BELA
Lazar KOJIC1 and Dillyara AJGOZINA2
1Maize Research Institute, Zemun Polje, Belgrade, Serbia
2SPK "Budan", Almatinska 51, p. Balatay, Kazakhstan
Abstract
Kojic L. and Dillyara Ajgozina (2008): Breeding and genetic properties of the maize variety Uzbekska bela (Uzbekh white). - Genetika, Vol. 40, No1, 17-21.
The Uzbekska bela (Uz.b.) maize variety was crossed to nine inbred lines and genetic difference between this variety and observed maize inbreeds lines was determined. The Uz.b. variety is a heterotic pair interesting for deriving maize hybrids with inbreds of BSSS and Lancaster origin, as well as, with some Yugoslav maize populations (Konjski zub, Domaći tvrdunac).
The inbred lines originating from Zambia showed somewhat lower yielding and heterosis percentage in relation to other observed inbred lines in hybrid combinations with the variety Uz.b.
Key words: grain yield, heterosis, heterotic pair.
Corresponding author: Kojic Lazar, Maize Research Institute, Slobodana Bajica 1, 11185 Belgrade-Zemun, Serbia
Published in „GENETIKA“ Vol. 40, No.1 (2008), pp. 23-30
© 2008 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade - Zemun
Serbia
UDC 575: 635.652
Original scientific paper
DIVERGENCE IN THE DRY BEAN COLLECTION BY PRINCIPAL COMPONENT ANALYSIS (PCA)
Mirjana VASIĆ, Jelica GVOZDANOVIĆ-VARGA
and Janko ČERVENSKI
Institute of Field and Vegetable Crops, Novi Sad, Serbia
Abstract
Vasić M., J. Gvozdanović-Varga and J. Červenski (2008): Divergence in the dry bean collection by principal component analysis (PCA).– Genetika, Vol. 40, No. 1, 23 - 30.
We studied the divergence of our beans collection. The study included two qualitative traits, grain color and shape, and 13 quantitative traits, namely three components of plant height, five direct yield components and five chemical properties of grain. The principal component analysis (PCA) showed which of the traits were decisive in genotype differentiation. The principal components were formed based on the correlation matrix and shown through unrotated and rotated values of trait correlation with the main axes. The percentage contribution of particular principal components to total variability was shown, as was the accumulation of variability. The variability of the collection was interpreted based on the seven principal components, the first one describing genotype productivity, the second grain shape, the third grain size, the fourth genotype harvestability and the last three describing the chemical composition of grain.
Key words: divergence, qualitative and quantitative traits, PCA, Phaseolus vulgaris
Corresponding author: Mirjana VASIĆ, vasicka@ifvcns.ns.ac.yu, Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad, Serbia
Published in „GENETIKA“ Vol. 40, No.1 (2008), pp. 31-38
© 2008 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade - Zemun
Serbia
UDC 575.22: 633.11
Original scientific paper
EVALUATION OF WHEAT RHT GENES USING MOLECULAR MARKERS
Biljana TOŠOVIĆ-MARIĆ 1, Borislav KOBILJSKI 2, Dragana OBREHT1
and Ljiljana VAPA 1
1Faculty of Natural Sciences, University of Novi Sad, Republic of Serbia
2Institute of Field and Vegetable Crops, Novi Sad, Republic of Serbia
Abstract
Tošović-Marić B., B. Kobiljski, D. Obreht and Lj. Vapa (2008): Evaluation of wheat Rht genes using molecular markers.– Genetika, Vol. 40, No. 1, 31-38.
Using STSs and SSR markers, three worldwide the most important major height reducing genes, Rht-B1b, Rht-D1b and Rht8 were evaluated in this paper. In the analysed set of hexaploid wheat, composed of 172 genotypes originated from more than 20 countries, Rht-B1b, Rht-D1b and Rht8 were found in 40%, 22% and 62% of cases, respectively. In genotype groups of domestic and foreign origin, the highest difference in allele frequency was determined in the case of Rht8, which was more frequent in domestic genotypes (78, 3%). The Rht-B1b was dominantly present in foreign germplasm (57, 6%). Portion of Rht-D1b was almost equal with 22, 6% in domestic and 21, 2% in foreign varieties. Obtained results and accepted methodology for detection of these three, the most important Rht genes, represent great start point for Marker Assisted Selection (MAS) for high yielding wheat genotypes in agro climatic conditions of Serbia and Mediterranean area.
Key word: Rht genes, molecular markers, wheat
Corresponding author: Tošović-Marić Biljana, Faculty of Natural Sciences, University of Novi Sad, Trg Dositeja Obradovića 2, 21000 Novi Sad, Republic of Serbia; Fax: +380 21 450 620, Tel. +380 21 63 64 902; E-mail: delfinb@neobee.net
Published in „GENETIKA“ Vol. 40, No.1 (2008), pp. 39-49
© 2008 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade - Zemun
Serbia
UDC 575: 633.11
Original scientific paper
MARKER ASSISTED SELECTION IN BMQ RELATED BREEDING PROGRAMS
Dragana OBREHT 1, Borislav KOBILJSKI 2, Mihajla ĐAN 1 and Ljiljana VAPA 1
1Faculty of Sciences, Novi Sad, Serbia
2Institute of Field and Vegetable Crops, Novi Sad, Serbia
Abstract
Obreht D., B. Kobiljski, M. Đana and Lj. Vapa (2008): Marker assisted selection in BQM related breeding programs. – Genetika, Vol. 40, No. 1, 39-49.
Implementation of marker assisted selection (MAS) in bread making quality (BMQ) oriented breeding programs could allow genetic potential assessment of genotypes prior to their phenotypic evaluation. The mechanisms underlying some quality traits in wheat are now understood. This knowledge, coupled with the availability of the DNA sequences of the genes encoding gluten proteins and the wide application of the PCR, has enabled the design of diagnostic DNA markers for these quality traits. Bread wheat breeding programs developed in Institute of Field and Vegetable Crops, Novi Sad have originated a wide range of quality cultivars with strong flours and hard grain texture. During twenty years, in the process of bread-making quality prediction, composition of HMW glutenin subunits were analysed beside standard technological parameters. However, in order to improve our breeding strategies new generations of PCR-based BMQ related markers were included in selection programs. This paper provides an overview of diagnostic DNA markers that are currently in use in foreign and domestic wheat selection programs.
Key word: wheat, quality, MAS
Corresponding author: Obreht Dragana, Faculty of Sciences, Department of Biology and Ecology, Trg D. Obradovica 2., 210000 Novi Sad,
Phone: 021 485 2656, E-mail: obrehtd@ib.ns.ac.yu
Published in „GENETIKA“ Vol. 40, No.1 (2008), pp. 51-65
© 2008 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade - Zemun
Serbia
UDC 575: 633.15
Original scientific paper
A NINE-BASE PAIR DELETION DISTINGUISHES TWO EN/SPM TRANSPOSON ALLELES IN MAIZE: THEIR GENETIC ACTIVITY AND MOLECULAR DESCRIPTION
Adriane MENSSEN and Peter A. PETERSON
Dept. of Agronomy (Plant Genetics), Iowa State University, Ames, USA
Abstract
Menssen A. and P.A.Petersona (2008): A nine-base pair deletion distinguishes two En/Spm transposon alleles in maize:their genetic activity and molecular description.– Genetika, Vol. 40, No. 1, 51-65.
Two En/Spm-transposable element alleles of the A1 locus in maize (Zea mays) are described. One of the alleles is al-m (papu), (Peterson, 1961). The distinctive phenotype of this allele is characterized with pale and purple sectoring amidst large areas of no sectoring. The other allele, al-m (Au), appears full colored but is heavily mutating and expresses large colorless areas. These two alleles differ in the frequency of derivative products [al-m( papu)-colorless and pale exceptions vs al-m(Au)-mostly colorless exceptions]. A molecular description is provided in an attempt to explain these differences in phenotypes and derivative products. A nine-base-pair deficiency in Exon 2 of the A1 locus of the a1-m (papu) allele originated following the origin of this allele and this deficiency is likely responsible for the differential phenotypes. The possible origin of this nine-base-pair deletion is discussed.
Key word: Al locus; En/Spm unstable alleles; induced deletions, transposable, transposable elements
Corresponding author: Peter A. Peterson, Dept. of Agronomy (Plant Genetics)
Iowa State University, Ames, IA 50011 USA, Ph 515-294-9652, Fax 515-204-2299
e-mail pap@iastate.edu
Published in „GENETIKA“ Vol. 40, No.1 (2008), pp. 67-74
© 2008 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade - Zemun
Serbia
UDC 575
Original scientific paper
NUTRIGENOMICS
Snežana B.PAJOVIĆ
Vinča Institute of Nuclear Sciences, Belgrade, Serbia
Abstract
Pajović B. S. (2008): Nutrigenomics. – Genetika, Vol. 40, No. 1, 67-74.
Nutritional genomics, or nutrigenomics, is a promising multidisciplinary field that focuses on studying the interactions between nutritional factors, genetic factors and health outcomes. Its goal is to achieve more efficient individual dietary intervention strategies aimed at preventing disease, improving quality of life and achieving healthy aging. Scientific progress in nutrition, medical and food sciences is having an increasingly profound impact on consumer’s approach to nutrition. There is a growing awareness that many chronic diseases are caused by unbalanced diet. In addition to disease prevention, the role of food as an agent for improving health has been proposed and a new class of food, so called functional food, has come into being. This term is used to indicate a food that contains some health-promoting components and not only traditional nutrients. From this point of view we could argue that many nutritional products belong to the family of functional food replete with bioactive peptides, antioxidants, probiotic bacteria, highly absorbable calcium, conjugated linoleic acid and other biologically active components.
Knowledge gained from comparing diet/gene interactions in different populations may provide information needed to address the larger problem of global malnutrition and disease.
Key word: genetic effects, food, disease
Corresponding author: Prof. Dr. Snežana B. Pajović, Laboratory of Molecular Biology and Endocrinology, Vinča Institute of Nuclear Sciences, P. O. Box 522 ,11001 Belgrade
Serbia,Tel./Fax : +381 (11) 2455 561 ; +381 (11) 80 66 514,E-mail: pajovic@vin.bg.ac.yu
Published in „GENETIKA“ Vol. 40, No.1 (2008), pp. 75-82
© 2008 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade - Zemun
Serbia
UDC 575: 633.11
Original scientific paper
TOLERANCE OF WHEAT SEEDLINGS TO EXCESS BORON
Milka BRDAR 1*, Ivana MAKSIMOVIĆ 1,2, Borislav KOBILJSKI 1, Tijana ŠKORIĆ ZEREMSKI 1 and Marija KRALJEVIĆ BALALIĆ 2
1Institute of Field and Vegetable Crops, Novi Sad
2Faculty of Agriculture, University of Novi Sad, Novi Sad
*scholarship holder of the Ministry of Science of Republic of Serbia
Abstract
Brdar M., I. Maksimović, B.Kobiljski, T. Škorić Zeremski and M. Kraljević Balalić (2008): Tolerance of wheat seedlings to excess boron. – Genetika, Vol. 40, No. 1, 75 -82.
Present in excessive amounts, micronutrient boron may become toxic for plants. The aim of this study was to investigate boron tolerance in seedlings of 12 wheat (Triticum aestivum L.) genotypes treated with boric acid. Selection criterion was root growth suppression in the presence of boron, which varied between 15.2 (Apache) and 46.3% (Renan). Root lengths on control and root growth suppression on treatments were not correlated. Boron content and dry weight of seedlings varied in vast intervals in all groups on control, as well as on treatments, which is probably caused by different boron tolerance mechanisms. Root growth suppression in genotypes Apache, Trakija and Bezostaja 1 was less than 20% and they may be considered as boron tolerant. Renan, Fundulea 4, Magdalena, Pergamino Gaboto and Donjecka 48 are marked as boron sensitive owing to more than 30% shorter roots on boron treatments. Norin 10/Brevor 14, Radika, Žitarka and Mironovska 808 were medium tolerant to excess boron.
Key word: wheat, boron tolerance
Corresponding author: Brdar Milka, Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad, 064/61 33 426 milkabrdar@yahoo.com
Published in „GENETIKA“ Vol. 40, No.1 (2008), pp. 83-93
© 2008 Serbian Genetics Society
S. Bajića 1, 11185 Belgrade - Zemun
Serbia
UDC 575.22: 633.15
Original scientific paper
s1 families as a source of beneficial alleles for breeding drought tolerant maize genotypes
Ksenija MARKOVIĆ, Ana NIKOLIĆ, Dragana IGNJATOVIĆ-MICIĆ,
Violeta ANĐELKOVIĆ and Vesna LAZIĆ-JANČIĆ
Maize Research Institute „Zemun Polje“, Belgrade, Serbia
Abstract
Marković K, A. Nikolić, D. Ignjatović-Micić, V. Anđelković and V. Lazić-Jančić (2008): S1 families as a source of beneficial alleles for breeding drought tolerant maize genotypes.– Genetika, Vol. 40, No. 1, 83 -93.
As water resources for agronomic use become more limiting development of drought tolerant genotypes become increasingly more important. In maize, under drought stress conditions, an increase in the length of anthesis-silking interval and a decrease of grain yield is observed. Different strategies have been proposed to overcome negative effects of drought on maize. Breeders at Maize Research Institute (MRI) have created and used adapted drought tolerant population - DTP-A (DTP79 x DTP12 x Dwarf), that was shown to be a good source of drought tolerance for locally adapted germplasm. In this research s1 families from DTP-A x B84 were scored for anthesis-silking interval and genotyped with SSR markers using BSA approach, with the aim to create a new gene pool for breeding drought tolerant hybrids. SSR analysis was performed in order to identify putative genomic segments and allele pattern differences responsible for expression of ASI, thus providing an insight into the genetic structure of s1 families. Four SSR loci, different in allelic structure between short and long ASI families, were identified. The main characteristic of these differences was the presence or absence of DTP79/DTP12 alleles. It seems that combination of several different genes and alleles influence ASI expression, with DTP79 alleles being beneficial and DTP12 alleles detrimental. Two chromosome regions identified in this work were congruent with qASI regions reported in Maize Genetics and Genomics Database. Clustering revealed that all sASI families grouped with DTP79, but one of the long ASI grouped with these genotypes too. This could indicate that, although total participation of DTP79 genome was similar in all the analyzed families, only a small portion of its genome influence anthesis-silking interval.
Key words: ASI, BSA, DTP, maize, SSR
Corresponding author: Vesna Lazić-Jančić, Maize Research Institute „Zemun Polje“
S. Bajića 1, 11185 Belgrade, Serbia
Tel: 011/3756704, Fax: 011/3756707, e-mail: vlazic@mrizp.co.yu