GENETIKA, Vol

GENETIKA, Vol. 29, No.1 (1997)

Snežana MLADENOVIĆ-DRINIĆ, Kosana KONSTANTINOV, Jovan ĐORĐEVIĆ and Ilija ŠATARIĆ

HETEROSIS AND COMBINING ABILITY FOR GRAIN YIELD IN MAIZE INBRED LINES [Abstract]

J.N. SFAKIANAKIS, N.D. KATSADONIS and G.L. EVGENIDIS

GCA AND SCA EFFECTS AND THEIR RELATIONSHIP WITH THE ORIGIN OF INBRED LINES IN MAIZE (Zea mays L.) [Abstract]

Gordana ŠURLAN-MOMIROVIĆ, Zorica TOMIĆ, Ivana RALEVIĆ and Nebojsa RALEVIĆ

EVALUATION OF A GERMPLASM COLLECTION OF Agrostis stolonifera L. THROUGH MULTIVARIATE ANALYSIS [Abstract]

Zoran TOMOVIĆ, Sasa ORLOVIĆ, Vojislav GUZINA and Petar IVANIŠEVIĆ

COMPARATIVE INVESTIGATION OF GROWTH RATE AND LENGTH OF THORNS OF BLACK LOCUST (Robinia pseudoacacia L.) [Abstract]

Veselinka ZEČEVIĆ, Desimir KNEZEVIĆ, Milanko PAVLOVIĆ and Danica MIĆANOVIĆ

GENETIC ANALYSIS OF YIELD COMPONENTS IN WINTER WHEAT (Triticum aestivum ssp. vulgare L.) [Abstract]

B.N. SINGH and Seema SISODIA

EVIDENCE FOR RARE-MALE MATING ADVANTAGE IN DROSOPHILA BIPECTINATA [Abstract]

S.K. BHARGAVA and R.K. DATTA

HETEROSIS AND COMBINING ABILITY OF SILK YIELD CONTRIBUTING CHARACTERS IN THE SILKWORM, BOMBYX MORI LINN. [Abstract]

Milija VELJKOVIĆ, Ivan TULIĆ and Ljiljana VOJVODIĆ

MODERN CYTOGENETIC DIAGNOSTICS OF HUMAN GAMETES AND EMBRYOS [Abstract]

Published in „GENETIKA“ Vol. 29, No.1 (1997), pp. 1-7

Serbia

UDC 575.222.7:633.15

Original scientific paper

HETEROSIS AND COMBINING ABILITY FOR GRAIN YIELD IN

MAIZE INBRED LINES

Snežana MLADENOVIĆ-DRINIĆ, Kosana KONSTANTINOV,

Jovan ĐORĐEVIĆ and Ilija ŠATARIĆ

Maize Research Institute ,,Zemun Polje", Belgrade-Zemun, Yugoslavia

Abstract

Mladenović-Drinić S., K. Konstantinov, J. Đorđević and I. Šatarić (1997): Heterosis and combining ability for grain yield in maize inbred lines - Genetika, Vol. 29, No. 1, 1-7.

Combining ability and heterosis for grain yield were studied in five maize inbred lines by the method of diallel crossing. The results obtained show that additive gene effects on grain yield were the highest, though the non-additive gene effects were statistically significant. The maize inbred line HD120-2-4, with the highest general combining effects, was included as a parental inbred in four hybrid combinations with high heterotic effect for grian yield. Based on the analysis of variance of generation means, after the model of EBERHART and GARDNER (1996), values obtained for the average heterosis and additive epistasis were highly statistically significant.

Key words: heterosis, combining ability, maize inbred lines

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Published in „GENETIKA“ Vol. 29, No.1 (1997), pp. 9-14

Serbia

UDC 575:633.15

Original scientific paper

GCA AND SCA EFFECTS AND THEIR RELATIONSHIP WITH

THE ORIGIN OF INBRED LINES IN MAIZE (Zea mays L.)

J.N. SFAKIANAKIS, N.D. KATSADONIS and G.L. EVGENIDIS

National Agricultural Research Foundation
Cereal Institute 57001 Thermi-Thessaloniki, Greece

Abstract

Sfakianakis J.N., N.D. Katsadonis and G.L. Evgenidis (1997): GCA and SCA effects and their relationship with the origin of inbred lines in maize (Zea mays L..). - Genetika, Vol. 29, No. 1, 9-14.

A total of 126 diallel sets of maize developed by the Cereal Institute of Thessaloniki within the maize breeding programme during the years from 1967 to 1983, were analyzed for general and speeific combining ability (GCA ,SCA) effects. In 78% of those diallel sets the GCA was significant at the 0.01 level of significance and in a further 9% in was significant at the 0.05 level of significance. The corresponding percentages for the SCA were 40% and 12%. Thus, general combining ability seems to be more important than SCA in maize. The genes with additive action seem to play a more important role in the expression of yield than genes with non additive action. The genetic material from which the inbred lines were developed was not of primary importance for the magnitude of GCA and SCA except that of unimproved material which seemed to have a significant effect on the magnitude of SCA

Key words: general combining ability, specific combining ability, origin of inbred lines, maize

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Published in „GENETIKA“ Vol. 29, No.1 (1997), pp. 15-22

Serbia

UDC 575:519.237

Original scientific paper

EVALUATION OF A GERMPLASM COLLECTION OF Agrostis

stolonifera L. THROUGH MULTIVARIATE ANALYSIS

Gordana ŠURLAN-MOMIROVIĆ¹, Zorica TOMIĆ², Ivana RALEVIĆ¹and Nebojsa RALEVIĆ¹

¹Faculty of Agriculture, University of Belgrade, 11000 Belgrade,

²Institute for Agriculture “Serbia”, Centre for Forage Crops,

37000 Kruševac, Yugoslavia

Abstract

Šurlan-Momirović G., Z. Tomić, I. Ralević and N. Ralević (1997): Evaluation of a germplasm collection of Agrostis stoloniferu L. through multivariate. analysis. - Genetika, Vol. 29, No. 1, 15-22.

Thirty five autochthonous populations of the species Agrostis stolonif.ni L. collected from a narrow Serbian region were subjected to both univariate and multivariate analysis. The plants were collected from various habitats: valley meadows – 22 populations, hilly meadows - 7 populations and mountain meadows and pastures - 6 populations. The populations of the species A. stolonifera L. exhibited three levels of polyploidness: diploid, tetraploid and hexaploid. The effect of the eleven most important quantitative characters was examined. The following characters were recorded during 1992 and 1993 on a single plant basis. In order to establish a taxonomic connection among germplasm accessions, similar populations were grouped in three clusters. The first cluster was formed by 12, the second by 9 and the third by 14 populations. All clusters represented homogeneous groups. It was not possible to differentiate between the 35 populations on the basis of their ploidy level and their habitats (valley, hilly, mountain meadows and pastures).

Key words: autochthonous populations, univariate analysis, multivariate analysis

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Published in „GENETIKA“ Vol. 29, No.1 (1997), pp. 23-30

Serbia

UDC 575

Original scientific paper

COMPARATIVE INVESTIGATION OF GROWTH RATE AND

LENGTH OF THORNS OF BLACK LOCUST (Robinia pseudoacacia L.)

Zoran TOMOVIĆ¹, Saša ORLOVIĆ², Vojislav GUZINA²

and Petar IVANIŠEVIĆ²

¹Research Development Center, Forest Enterprise ,,SRBIJAŠUME",11000 Beograd

²Poplar Research Institute, 21000 Novi Sad, Yugoslavia

Abstract

Tomovic Z., S. Orlović, V. Guzina and P. Ivanišević (1997): Comparative, investigation of growtli rule, and length of thorns of black locust (Robnia pseudoacacia L.). - Genetika, Vol. 29, No. 1, 23-30.

Investigations of the possibility for selection of black locus (Robinia pseudocacia L.) in order to increase the increment of wood volume, improve the quality of the trunk and variability in the length of thorns was carried out in a series of experiments with provenances, generative and vegetative progenies. Statistically significant differences in heights were found between the clones, which were also considerably different from generative progenies. No statistically significant differences were found between tested generative progenies, which are the result of the relatively low number of analyzed progenies whose parent trees were selected primarily on the basis of the quality of the trunk, and after that according to the wood volume increment. The very high values of hereditary coefficient in a broad (h²bs = 0.792) and narrow sense (h²ns = 0.796) for height increment point at the high degree of genetic control of this property. Statistically significant differences between progenies were found when the length of thorns was studied. The very high value for hereditary coefficient in a broad (h² bs = 0.917) and narrow sense (h² ns = 0.950) suggests that this property is mostly genetically influenced. On the basis of the data obtained it can be concluded that considerable possibilities exist for breeding of black locust by selection.

Key words: black locust, heritability, growth rate, length of thorns, breeding

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Published in „GENETIKA“ Vol. 29, No.1 (1997), pp. 31-40

Serbia

UDC 631.523.11.633

Original scientific paper

GENETIC ANALYSIS OF YIELD COMPONENTS IN WINTER WHEAT

(Triticum aestivum ssp. vulgare L.)

Veselinka ZEČEVIĆ, Desimir KNEZEVIĆ, Milanko PAVLOVIĆ and Danica MIĆANOVIĆ

Agricultural Research Institute ,,SERBIA", Center for Small Grains, 34000, Kragujevac, Yugoslavia

Abstract

Zečević V., D. Knežević, M. Pavlović and D. Mićanović (1997): Genetic analysis of yield components in winter wheat (Triticum aestivum ssp. vulgare L.) - Genetika, Vol. 29, No. 1, 31-40.

The mode of inheritance, gene effect, combining ability and genetic components of variance for length of spike, number of spikelets per spike and number of kernels per spike in 4 divergent cullivars (Srbijanka, Partizanka, KG-56 and PKB-111) were studied in diallel crosses (without reciprocals). Cuitivars and fi hybrids showed great differences for the analyzed traits. This study showed that the mode of inheritance for all analyzed yield components was different with preponderance of partial dominance. The best general combiner for length of spike was Partizanka cultivar, while for number of spikelets per spike and number of kernels per spike it was Srbijanka cultivar.

Key words: wheat, cultivar, length of spike, number of spikelets/spike, number of kernels/spike, combining ability, inheritance

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Published in „GENETIKA“ Vol. 29, No.1 (1997), pp. 41-48

Serbia

UDC 596.773.4

Original scientific paper

EVIDENCE FOR RARE-MALE MATING ADVANTAGE IN

DROSOPHILA BIPECTINATA

B.N. SINGH and Seema SISODIA

Genetic Laboratory, Department of Zoology, Banaras Hindu University,

Varanasi-221005, India

Abstract

Sing H.N. and Seema Sisodia (1997): Evidence for rare-male mating advantage in Drosophila bipectinata. - Genetika, Vol. 29, No. 1. 41-48.

In order to detect a rare-male effect in Drosophila bipeclinata the mating success of wild type and cut wing mutant males was studied in an Elens-Wattiaux mating chamber at 9 different ratios. The female-choice technique was employed in all the experiments. Wild type-males were tested with cut wing mutants. The results showed that both types of males were equally successful in mating when present in the same ratio but they were more successful in mating when in a minority. When the males became common in the mating chamber, this advantage disappeared. Thus the present results provide evidence for the existence of a rare-male mating advantage in D. bipectinata.

Key words: Drosophila bipectinata, wild type, ct mutants, mating success, rare-male effect

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Published in „GENETIKA“ Vol. 29, No.1 (1997), pp. 49-62

Serbia

UDC 631.527.52

Original scientific paper

HETEROSIS AND COMBINING ABILITY OF SILK YIELD CONTRIBUTING CHARACTERS IN THE SILKWORM, BOMBYX MORI LINN.

S.K. BHARGAVA and R.K. DATTA

Central Sericultural Research and Training Institute Mysore -570 008, India

Abstract

Bhargava S.K., and R.K. Datta (1997): Heterosis and combining ability of silk yield contributing characters in the silkworm Bombyx mori Linn, - Genetika, Vol. 29, No. 1, 49-62.

The combining ability effects of five races (KA, CC1, CA2, NB4D2 and NB18) of the silkworm (Bombyx mori Linn) and their 20 F₁'_s, including reciprocals were analyzed in a 5 x 5 diallel crossing system for eight silk yield contributing characters (pupation rate, cocoon yield, cocoon weight, shell weight, raw silk percentage, silk filament length, silk reliability and silk neatness). The parental race CA2 did not show the best general combining ability (GCA) for any of these eight characters, whereas that shown by the remaining parental races for different characters was as follows: KA (pupation rate, raw silk percentage, silk filament length, silk reliability and silk neatness); CC1 (cocoon weight and shell weight); NB4D2 (cocoon yield, cocoon weight and raw silk percentage); NB18 (pupation rate). GCA values of CA2 were negative for pupation rate, cocoon yield, cocoon weight, shell weight, raw silk percentage and were low for silk filament length, silk reliability and silk neatness. This indicates its poor performance in hybrid combinations with the remaining races. The results of specific combining ability (SCA) suggest restricted use of CA2 as a female parent in hybrid combinations with the NB18 or NB4D2 males. CA2 x NB18 showed desired positive SCA effects for all the characters except silk neatness, whereas CA2 x NB4D2 showed desired positive SCA effects for all the characters except pupation rate and silk reliability, CC1 x NB4D2 showed desired positive SCA effects for all the characters. The estimates of general and specific combining ability variances revealed that the non-additive type of gene control plays a predominant role in the inheritance of all the characters except cocoons weight. In case of cocoon weight the gene control is of additive type. The importance of general and specific combining ability in identifying superior silkworm races for improving the silk yield contributing characters in future breeding programmes is discussed.

Key words: Cocoon weight, cocoon yield, silk yield contributing characters, gene control, variances

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Published in „GENETIKA“ Vol. 29, No.1 (1997), pp. 63-68

Serbia

UDC 653.13

Review article

MODERN CYTOGENETIC DIAGNOSTICS OF HUMAN

GAMETES AND EMBRYOS

Milija VELJKOVIĆ, Ivan TULIĆ and Ljiljana VOJVODIĆ

Institute of Gynecology and Obstetrics, 11000 Belgrade, Yugoslavia

Abstract

Veljković M, I. Tulić, and Lj. Vojvodić (1997): Modem cytogenetic diagnostics oj human gametes and embryos. - Genetika, Vol. 29, No. 1, 63-68.

The fluorescent in sity hybridization (FISH) technique using chromosome specific probes, allows targeted detection of numerical and structural chromosome aberrations in metaphasc chromosomes and interphase nuclei. Improved DNA decondensation procedures resulted in sperm cells with intact morphology of both the head and the tail, which allowed the performance of efficient in situ hybridization. At the present time, the data suggest the possibility of detecting chromosomal aneuploidy in oocytes through cytogenetic analysis of their corresponding first polar body by FISH as a possible approach for preimplantation diagnosis of major chromosomal aberrations. The FISH technique can be widely applied for the detection of the genotype of human embryos and in preimplantation diagnosis. In this review, special attention is paid to the relevance of preimplantation diagnosis by fluorescent in sity hybridization.

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