Serbian Genetics Society
THE FIRST CONGRESS OF SERBIAN GENETICISTS
Vrnjačka Banja, Serbia June 08-11, 1994
Serbian Genetics Society
THE FIRST CONGRESS OF SERBIAN GENETICISTS
Vrnjačka Banja, Serbia June 08-11, 1994
Chairmanship
Vukica DIKLIĆ
Janko DUMANOVIĆ
Dragoslav MARINKOVIĆ
Aleksandar KRSTIĆ
Ana SAVIĆ
Bogosav SOLDATOVIĆ
Aleksandar TUCOVIĆ
Organizing Committee
Kosana KONSTANTINOV, president
Marija Kraljević-Balalić
Nada BARJAKTAREVIĆ, secretary
Marko Anđelković
Dragan ALAVANTIĆ
Vasilije Isajev
Mile IVANOVIĆ
Vesna LAZIĆ-JANČIĆ
Miroslava MICIĆ
Teodora PAPIĆ-PALJIĆ
Gojko SAVIĆ
Draga SIMIĆ
Vitomir VIDOVIĆ
Ljubiša TOPISIROVIĆ
Branka TUCIĆ
Secretariat
Miodrag DIMITRIJEVIĆ
Jelena KNEŽEVIĆ
Desmir KNEŽEVIĆ
Vera ROBOVIĆ
Goran SARATLIĆ
Ljiljana VAPA
Mladen VUJOŠEVIĆ
Serbian Genetics Society
THE FIRST CONGRESS OF SERBIAN GENETICISTS
Vrnjačka Banja, Serbia June 08-11, 1994
Symposium 1. CYTOGENETICS
B. Soldatović
A retrospective evaluation of development of cytogenetics
Jovanka Atlagić
A cytogenetic study of interspecific sunflower hybrids (H. annum L. x H. occidentalis Riddelt)
Jelena Blagojević i Vujošević M.
N. Đelić, B. Soldatović i M. Anđelković
Svetlana Fišter i B. Soldatović
Drinka Merčap, A. Đujić, Z. Magić i M. Simović
Different responses to genotoxic agents depending on the genetic constitution
Snežana Mezei, L. Kovačević i N. Čačić
The effect colchicine on some quantitative characters of sugar beet
B.P. Pavlović
A method for making numerical karyotype etalons
B.P. Pavlović
Numerical karyotype etalons Erinaceus europaeus europaeus
B. Pavlović, i Nevenka Pavlović
Slavica Simović i G. Marković
The chromosome complements of some fish species from the Cyprinidae family
Z. Stanimirović, Mirjana Vučinić, B. Soldatović, E. Herzog
S. Šesek
N. Tanić, Olivera Spasić, Jelena Blagojević i M. Vujošević
Chromosomes of frogs from the family Ranidae (Anura, amphibia) - a comparative analysis
Marijana Vučinić, Z. Stanimirović, B. Soldatović
"ROSOL", as an eventual inducor of polyploid type chromosome aberrations
M. Vujošević i Jelena Blagojević
Symposium 2. MEDICAL GENETICS
Slavenka Adžić, Stefanija Marković, D. Plećaš, N. Radunović i Z. Pilić
Chromosome aberrations found by cordocentesis
Nevenka Aleksić
In vitro genotoxicological characterisation of ivermectin and praziquantel
M. Babić i M. Micić
A cephalometric analysis of patients with Klinefelter's syndrome
S. Branković i G. Savić
Suzana Cvjetičanin i D. Marinković
N. Dedović, J. Milašin, V. Petrović, G. Stamenković, M. Gavrić, Z. Nikolić
A high incidence of H-ras gene point mutations in two types of head and neck neoplasias
Vukosava Diklić, Marija Kosanović, Milica Todorić, M. Vukotić, S.P. Stojković
The role of genetic factors in the etiopathogenesis of cardiovascular disorders
Nada Dimković, Vitana Kostić, Dragan Jovanović i Nada Barjaktarović
Cytogenetic analyses reveal "atypical cells" in the peritoneal dialysis effluent
B. Đorđević, D. Mihailović, D. Stojanović, C. Kutlešić, M. Čekerevac, Z. Popović i Z. Stanković
Histologic findings in the ovary of a Turner's syndrome patient
Slobodanka Grković i Vesna Ivanović-Deretić
A report of a 9p/10p family translocation
Marija Guć-Šćekić, Dragana Anđelković, V. Jurukovski i Gordana Pilić-Radivojević
Genetic determinants in the prognosis of neuroblastoma
Vesna Ivanović-Deretić, Slobodanka Grković i B. Garzičić
A report of autosomal whole arm translocation
Zorica Janakova
Zorica Janakova
Zorica Janakova
Humoral factors in the variability of allergic reactivity
Gordana Jokšić
Dragana Jovanović i D. Marinković
J. Jovanović-Privrodski, A. Krstić, F. Popić-Paljić, R. Aleksić i G. Raca
J. Jovanović-Privrodski, A. Krstić, F. Popić-Paljić, Z. Pilić
Vesna Knežević, Vukosava Diklić, Marija Kosanović i N. Vojvodić
Genetic investigations of cardiovascular diseases
M. Krajinović, K. Ivanović, L. Mestroni, V. Diklić i J. Nikoliš
A. Krstić, M. Kovačević, G. Raca, F. Popić-Paljić, J. Jovanović-Privrodski, R. Aleksić, N. Čamber, R. Madžar, V. Čihi, Lj. Gaćina, N. Mitrovčan i Lj. Malešević
The registration of chromosomal aberrations using the methodology of D.S.Borgoankar
M. Krstić, Stefanija Marković i Slavenka Adžić
Moral and ethical aspects of prenatal diagnosis in hereditary diseases
Živana Laca
Cytogenetic investigations in medical genetics
Milena Ludoski, Ljiljana Lalić, Vera Popović, V. Jurukovski i Angelina Novak
B. Lukić, Nada Barjaktarović, Nada Todorović i Vitana Kostić
Ljiljana Luković, Jelena Milašin, N. Arsenović i Vukosava Diklić
Chromosomal aberrations in three ovarian tumors
D. Marinković, D. Jovanović, S. Cvjetičanin i S. Branković
Genetic homozygosity and normal vs. pathological human variation
Miroslava Mićić
The influence of sex chromosomal anomalies on spermatogenesis
D. Mihailović, Z. Stanković, B. Đorđević, V. Mihailović i M. Čekerevac
The nuclear volume in Patau's syndrome
V. Mihailović, Z. Stanković, D. Mihailović i D. Stojanović
The frequency of acrocentric associations in women with spontaneous abortus
Jelena Milašin, Nasta Dedović, S. Mićić, B. Dimitrijević, Vukica Diklić
H-ras gene mutations in human urinary tract tumors
Olivera Milošević-Đorđević i Nada Barjaktarović
The reaction of human T limphocytes to mafosfamide and 5-fluorouracil in vitro
S. Minić, M. Kosanović i B. Petrović
A citogeneticinvestigation of chromosomes in patiens with incontinentio pigmenti
J. Nikoliš, V. Kekić, S. Mićić, V. Diklić, M. Ristanović
Ag- NORs in normal human population
Angelina Novak, Đ. Kokai, Vera Popović, Milena Ludoški, V. Jarukovski
Ivana Novaković, Olga Antonović, Slavenka Adžić i Svjetlana Maglajić
V. Novosel
A genetic survey of a family with repeated polydactylism
Vesna Pendić, Vera Popović i Angelina Novak
Familial 46,xy gonadal dysgenesis
D. Pešut, D. Marinković
ABO blood type frequency in some lung diseases
F. Popić-Paljić, A. Krstić, J. Jovanović-Privrodski, R. I Aleksić i G. Raca
Genetic counselling of married couples in cases of effects of harmful environmental noxes
F. Popić-Paljić, A. Krstić, J. Jovanović-Privrodski, G. Raca i R. Aleksić
The use of modern software in diagnosing rare syndromes
Branka Popović, B. Garzičić i Biljana Spremo
Fragile sites and oncogenes as a possible risk for neoplasia in carriers of balance translocations
G. Raca, A. Krstić, B. Garzičić, F. Popić-Paljić, J. Jovanović-Privrodski, R. Aleksić, N. Čamber, R. Madžar, V. Čihi, Lj. Gaćina, N. Mitrovčan i Lj. Malešević
An induction of fragile sites on human chromosomes by aphidicoline and cytosine-arabinoside
Dragica Radojković, Ana Savić, J. Savić, P. Minić i B. Brukner-Dabović
An application of the recombinant DNA method in the prenatal diagnostic of cystic fibrosis
S. Radulović, Nada Barjaktarović i Vitana Kostić
A rare variant of karyotype 47,XY,13 in AML (M2 type)
O. Ristić, Ž. Laća, S. Nikšić, N. Aranđelović i I. Timotijević
Biljana Spremo i B. Garzičić
L.N. Stojanović, E. L. Nassonov i M. Stanić
Nada Todorović, B. Lukić i Nada Barjaktarović
Cytogenetic parameters during different treatments in perinatology
Symposium 3. MOLECULAR GENETICS AND GENETIC ENGINEERING
Desanka Božin i Gordana Cerović
Desanka BOŽIN, Gordana CEROVIĆ i RADMAN
Desanka Božin, Gordana Cerović i RADMAN
Mismatch recognition efficiency. II. Mismatch recognition efficiency in the mammalian nucleus
Sanja Glišić, Ivana Savić i Alavanić
The use of multiallelic human apoB gene DNA polymorphism (3'HVR) for DNA typing
Sanja GLIŠIĆ, Ivana SAVIĆ i ALAVANTIĆ
Sanja Glišić, Ivana Savić i Alavantić
Sanja Glišić, Ivana Savić, Nadežda Radoja i Alavantić
An insertion/deletion polymorphism in the signal peptide of the apoB gene
Đ. Fira, M. Kojić, A. Banina i Lj. Topisirović
Extracellular proteinases of mesophilic lactobacilli
Radmila V. Ivanišević, Mirjana M. Milić, Dragana S. Ajdić, Milija Z. Jovičić, Mila J. Janković i J. Savić
Mila J. Janković, Mirjana M. Milić, Radmila V. Ivanišević, Z. Jovičić i J. Savić
Replication and mutagenesis: Analysis of a highly mutable sequence from the hisC gene of E. Coli
Z. Jovičić, Mirjana M. Milić, Radmila V. Ivanišević, Mila J. Janković i D. Savić
lieS and glnF (ipoN) mutants of E. coli show an increased resistance to novobiocin
Knežević-Vukčević, B. Čuljković, M. Vulović, B. Vuković-Gačić i D. Simić
The effect of bioantimutagens on intrachromosomal recombination in Escherichia coli K12.
M. Kojić, Lj. Topisirović
Kojić, Topisirović, Branka Vasiljević
Regulation of the expression of the sgm gene from Micromonospora zionensis
Kosana Konstantinov, Snežana Mladenović, Snezana Gošić, G. Saratlić, N. Delić, R. Petrović
Recombinant DNA technology in plant variety patenting and protection of authors rights
Konstantinović, Sanja Ivković i Glišin
Temperature - dependent activities of hybrid Escherichia coli promoters
Koviljka Krtolica, Nadežda Urošević, Dobrila Nešić, Ljiljana Tukić, Z. Magić i B. Dimitrijević
The activation of oncogenes in human myeloid neoplasta
Vesna Lazić-Jančić, D. Kovačević, A. Steed i S.A. Quarrie
Genotype-specific accumulation of BAR17MRNA in maize leaves during drought stress
Vesna Lazić-Jančić, C. Lebreton, A. Steed i S.A. Quarrie
Investigation on qtl for abscisic acid production using RFLP markers
Z. Magić, Svetlana Matić i G. Poznanović
Radiation induced expression of acute phase protein genes in rat liver
Vesna Maksimović, Svetlana Radović, Erika Varkonji i Ana Savić
Analysis of mRNAs in developing buckwheat seeds
Jelena Marjanović, V. Glišin i Zvezdana Popović
The anemic Belgrade rat 67kd erythrocyte membrane protein
N. Miladinov, B. Bojović, M. Kojić, A. Banina i Lj. Topisirović
Analysis of the regulator domain of the prt gene in Lactococcus lactis subsp. lactis BGIS29
Mirjana M. Milić, M.Z. Jovičić, Radmila V. Ivanišević, Mila J. Janković i J. Savić
A new cysB regulated gene that modifies novobiocin rezistance in cysB mutanats of Escherichia coli
Snežana Mladenović, Kosana Konstantinov, Bojana Tadić i G. Saratlić
The influence of bacterial on multiple allele polimorphism in maize
Sonja Pavlović, Tatjana Mitrović, Glišin i Zvezdana Popović
An analysis of the distal promoter of the rat bminy - globin gen
S. Radoja, M. Konstantinović i V. Glišin
Svetlana Radović, Vesna Maksimović i Ana Savić
Characterization of the 13 S globulin of buckwheat seed
Ivana Savić, Sanja Glišić, Nadežda Radoja i D. Alavantić
Ivana Savić, Sanja Glišić, Nadežda Radoja i D. Alavantić
S. Stanković, J. Knežević-Vukčević, B. Vuković-Gačić i D. Simić
An investigation of repair mechanisms in different Bacillus thuringiensis subspecies
Milena Stevanović i P.N. Goodfellow
Cloning and characterisation of the human SOX3 gene
I. Stojanović, M. Vujičić i Lj. Topisirović
A study of the segregational stability of plasmid pAl from Lactobacillus plantarum A112
J. Svirčević, M. Kojić, A. Banina i Lj. Topisirović
Nataša Trutić, Koviljka Krtolica, M. Čolić i Z. Mugić
Branka, M. Kojić, Lj. Topisirović
Resistance to hygromycin B in Micromonospora strains
Miloš Vujanac, Vesna Todorović, Snežana Kojić, Ana Savić i Dragana Stefanović
Extrachromosomal replication of plasmids in Drosophila embryos
Nataša Vukov, Snežana Kojić, Lj. Topisirović i Branka Vasiljević
Analysis of the pMZl plasmid from Micromonospora zionensis
Symposium 4. BREEDING OF ORGANISMS
D. S. Adamović, B. Pekić i Z. Lepojević
J. Boćanski, M. Stojaković
V. Bogdanović, D. Radojković, Radica Vidić-Dedović i P. Stojić
Methods for evaluating additive genetic effects
Jelena Bošković i M. Bošković
R. Cerović
A study of microgametogenesis in vitro in sour cherries
N. Čačić, L. Kovačev i Snežana Mezei
Combining abilities for the leaf area index of sugar beet (Beta vulgaris L.)
M. Dimitrijević, Sofija Petrović i Marija Kraljević-Balalić
Analysis of the gene effect in the inheritance of the number of kernels per spike in wheat
B. Dozet, R. Marinković
Recurrent phenotype selection of protein sunflower (Helianthus annum L. var. macrocarpus Ckll.)
G. Drinić, M. Ivanović i Jelena Vančetović
Genetic gain from selection based on hs progenies in two synthetic populations of maize
D. Đokić, S. Lomović, M. Milovanović i R. Ognjanović
D. Đokić, S. Lomović, M. Milovanović i R. Ognjanović
D. Đokić, S. Lomović, M. Milovanović i R. Ognjanović
D. Đokić, M. Kostić, S. Lomović i M. Jelić
Some parameters of mineral nutrition efficiency as an indicator in wheat selection
D. Đokić, M. Kostić, S. Lomović i M. Milovanović
The efficiency nitrogen nutrition as a genotype characteristic of wheat
D. Đokić, M. Kostić, S. Lomović i M. Milovanović
J. Đorđević i M. Ivanović
R. Đorđević, Z. Marković, V. Pešić i I. Đinović
The influence of "afila" genes on the forming of pods in Pisum sativum L.
Matilda Đukić i Dragica Obratov
Genetic specificity of morphological characters of hybrid plane half-sib progeny
Ž. Gajić i A.E. Bell
M. Grbić
Growth characteristsics of Siberian elm (Ulmus pumula L.) rooted cuttings from dwarf parent trees
Branislava Grbović i Biljana Nikolić
Differences between 6 varieties of mulberry (Morus alba, L.) propagated in vitro
Đ. Gvozdenović, Marija Kraljević-Balalić, I. Mihaljev i A. Takač
Components of genetic variance for the length and width of pepper fruits (Capsicum annuum L.)
V. Guzina, S. Orlović i G. Abramović
V. Isajev, A. Tucović i Mirjana Šijačić
Developments in the improvement of austrian pine (Pinus nigra Arn.) in Serbia
Đ. Jocković, J.R. Vilcox, H.J. Xu, Milica Hrustić i M. Stojaković
J. Joksimović, R. Marinković i M. Mihaljčević
Combining ability for yield components of F1 hybrids of sunflower (Helianthus annuus L.)
B. Jovanović, S. Prodanović i D. Stevanović
D. Knežević
Identification of wheat varieties by Gli- allele composition
D. Knežević, Mirjana Menkovska i Dragica Zorić
Allelic variation at Gli- loci in some Macedonian wheat cultivars
D. Knežević, M. Pavlović, Marija Kraljević-Balalić i Veselinka Zečević
Genetička analiza površine lista zastavičara kod pšenice (Triticum aestivum L.)
D. Knežević, Ljiljana Vapa i Aleksandra Yurievna Novoselskaya
Genetic analysis of the area of the flag leaf in wheat (Triticum aestivum L.)
L. Kovačev, N. Čačić i Snežana Mezei
Polu sib test kros rekurentna selekcija multigermnih tetraploidnih oprašivača šećerne repe
B. Kovačević, Snežana Mezei i Marija Kraljević-Balalić
Half-sib tests cross recurrent selection of multigerm tetraploid pollinators of sugar beet
Mirjana Kraljević-Balalić, M. Dimitrijević i Sofija Petrović
Combining ability analysis for grain number in wheat
M. Kuburović, M. Pavlović i D. Knežević
Combining ability and gene effects for root volume in wheat
Nevenka Kuprešanin i Snežana Mezei
Induction of haploid callus in sugar beet
D. Latinović, Lj. Lazarević, P. Stojić, G. Trifunović i M. Katić
Possibilities of the application of restricted selection indexes in dairy cattle populations
Vera Lavadinović, V. Isajev
The phenotype - physiological variability of 29 Douglas fir provenances in test plantations
Lj. Lazarević
Possibilities and limits in the genetic improvement of milk characteristics in cattle
Lj. Lazarević, D. Latinović, G. Trifunović, M. Katić i P. Stojić
The influence of the level of milk production on the genetic variation
Milomirka Madić i M. Pavlović
Inheritance of stem height in barley hybrids (Hordeum vulgare L.)
R. Marinković, D. Škorić, J. Crnobarac i N. Dušanić
Correlations and path analysis in castor beans (Ricinus communis L.)
Ljiljana Merkulov, Jadranka Ivezić i Marija Kraljević-Balalić
Danica Mićanović, Zora Sarić, M. Sarić i V. Mitrović
Genetic variability of wheat in making natural associations with free-living diazotrophs
N. Mišić R. Cerović i Gordana Đurić
Atrophy opovules in flowers with an interrupted development in the plum (P. domestica L.)
N. Mićić, R. Cerović i Gordana Đurić
Ovary deformities in plums due to irregular carpel fusion
M. Mihaljčević
Yellow spots on sunflower leaves (H. annum L.) are caused by nuclear and extranuclear factors
I. Mihaljev, Marija Kraljević-Balalić, P. Dorić i S. Petrović
Future needs for cchanges of conceptions and methods in plant breeding
M. Milutinović, Ružica Džamić, L. Avramov, D. Nikolić i M. Nikolić
M. Milutinović, Gordana Šurlan-Momirović, N. Ralević, Ivana Ralević i Vera Rakonjac
Multivariate analysis in different populations of wild sweet cherry (Prunus avium L.)
A.M. Mirković, R.R. Todorović, P.D. Mišić, Z.V. Pavlović i N.G. Zec
Selection of Persian wallnut, Juglans regia L. in Serbia
M. Mitrović
Economic and biological properties of some walnut selection
Nastasija Makovački, Snežana Mezei i L. Kovačev
Compatibility between sugar beet hybrids and Azotobacter strains
S. Orlović, V. Guzina
An assessment of the possibility of utilization of leaf-blade anatomic structure in poplar selection
Zorica Pajić i M. Babić
Popcorn hybrids - development and utilization
M. Pavlović, M. Kuburović, D. Knežević i Veselinka Zečević
Genetic effects and inheritance of the size of the sclerenchymous layer in the wheat stem
V. Pešić i Dragica Zorić
Inheritance of stem height in wheat hybrids of the F3 and F4 generations
R. Petrović, N. Delić i M. Vidaković
Sofija Petrović, Marija Kraljević-Balalić i M. Dimitrijević
The inheritance and phenotypic variability for the number of kernels per spike in wheat
M.M. Petrović, R. Lazarević, Lj. Lazarević S. Vasović, B. Miščević, S. Aleksić, D. Radosavljević
Genetical parameters of traits of body development in young female cattle
Lj. Prijić, Mirjana Jovanović i M. Mladenović
Application of genetic male sterility to the soybean breeding program
Vera Rakonjac, T. Živanović i D. Nikolić
Varijability and heritability components of some sweet cherry fruit characteristics
G. Saratlić, K. Konstantinov, I. Verešbaranji, Snežana Mladenović, J. Dumanović, B. Kerečki
Genetic control of the oil quality in the maize kernel
P. Sklenar, J. Boćanski i Marija Kraljević-Balalić
The mode of inheritance of leaf area in maize (Zea mays L.)
A. Stančević i M. Nikolić
M. Stanisavljević
In vitro germination and storage of blackberry pollen
Tatjana Stevanović Janežić i V. Isajev
M. Stojaković, D. Jocković, J. Boćanski, G. Bekavac i R. Popov
A study of combining abilities for grain yield in maize (Zea mays L.)
Vidosava Šećerov-Fišer
Mode of inheritance for plant height in ornamental sunflowers
Gordana Šurlan-Momirović, Zorica Tomić, Ivana Ralević i N. Ralević
Evalluation of germplasm collection of Agrostis stolonifera L. through multivariate analysis
Zorica Tomić
Improvement of italian ryegrass (Lolium multiflorum Lam.) by chromosome duplication
Z. Tomović i S. Orlović
The variability and heredity of leaf morphological parameters in poplar clones, section leuce
G. Trifunović, Lj. Lazarević , D. Latinović, P. Stojić i M. Katić
B. Trifunović, V. Trifunović, Gordana Radović i D. Jelovac
Selection progress and divergence in local synthetic populations of maize (Zea mays L.)
A. Tucaković i V. Isajev
Polyploid complexes, diploidization and improvement of trees and shrubs
A. Tucović, V. Isajev i Mirjana Šijačić
Sex variability and its significance in the improvement of the black poplar (Populus nigra L.)
A. Tucović, V. Isajev i Mirjana Šijačić
Mass and individual selection of the white bark pine (Pinus heldreichii Chrisst.) in Serbia
Ljiljana Vapa, S. Denčić, Erika Rakk-Šoltes i S. Kevrešan
RFLP at the Glu-1 loci and the bread-making quality of wheat cultivars
Mirjana Vasić
Genetic analysis of the number of seeds per plant in white bean crosses
Dragana Vasić, Ana Marjanović i B. Dozet
Dragica Vilotić, Mirjana Šijačić i V. Isajev
Jasmina Zdravković, Z. Marković, M. Damjanović i M. Zdravković
Inheritance of stoutness of the pericarp in tomato fruits (Lycopersicon escukntum Mill.)
Veselinka Zečević, M. Pavlović, D. Knežević i B. Vulić
Genetic analyses of productive tillering in wheat
Marija Zlokolica i I. Gerić
Isozyme variation among maize collections from Yugoslavia
T. Živanović, Gordana Šurlan-Momirović, Ivana Ralević i N. Ralević
Numerical taxonomy of ZPSin populations of maize based on quantitative traits
Symposium 5. POPULATION AND EVOLUTIONARY GENETICS
N. Tucić
Population genetics and two great syntheses of modern biology
I. Aleksić
Genetic components of variation in size and shape during ontogeny in the lizard Lacerta oxycephala
S. Avramov i Branka Tucić
Quantitative genetics of the juvenile traits in Iris Pumila: The structure of phenotypic variation
C. Cekuš
Presence of fossa mentalis and dimples in samples, from the area surrounding Subotica
G. Cekuš
Variations of hair limits on the forehead in samples from northern Serbia
Jelka Crnobrnja-Isailović i I. Aleksić
Ivana Gliksman
The quantitative genetic basis of increased longevity in the bean weevil (Acenlhoscelides obtectus)
V. Kekić, Sofija Pavković-Lučić, Aleksandra Čvoro i N. Milošević
Sexual selection and body size in Drosophila melanogaster
D. Knežević, D. Marinković, Lj. Agramović
A population genetic study of left-handedness in citizens of Prokuplje
Stanislava Mikuljanac i O. Stojković
Mirjana Milanović i M. Anđelković
Dragana Milanović i Sofija Karaulić
N. Milošević, V. Kekić, Sofija Pavković-Lučić i Aleksandra Čvoro
Learning in Drosophila melanogaster implications on the genetic structure
Milica Milošević, Z. Magić, Č. Radojičić i D. Marinković
V. Novosel
D. Paunović, R. Simonović, I. Stolić i M. Dokić
Calculating the risk factor for transfusion-associated graft-versus-host disease
B.P. Pavlović
The potential number of cells and system size of individuals and populations
R. Simonović, D. Paunović, I. Stolić i V.D. Miletić
Disequilibrium pattern analysis between A and B loci of the HLA system in Serbia
M. Stamenković-Radak, J.B. Clark i M. Kid Well
Snežana Stanić i D. Marinković
Selection for a different time until the first mating in Drosophila melanogaster
Vesna Stojiljković
Individual variability of a-amylase activity in Drosophila subobscura
O. Stojković
Darka Šešlija i D. Blagojević
Tatjana Terzić i M. Anđelković
Ljiljana Vapa, Ivana Karanović, Dragana Radović, J. Purger i M. Bokorov
The genetic distance between three species of the genus Apodemus
G. Živanović, Mirjana Milanović i M. Anđelković
Correlation of structural complexity of o chromosome and the genetic load in Drosophila suboboscura
Jelena Živanov-Čurlis i Estera Mrčarica
The effect of the aging on the in Drosophila melanogaster genetic load
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
A RETROSPECTIVE EVALUATION OF DEVELOPMENT OF CYTOGENETICS
B. SOLDATOVIĆ
Veterinarski fakultet, Beograd
Recent advances in general and animal cytogenetics, and the significance of fundamental and applied research results, as well as modern trends in genetic research were presented in this paper. A retrospective view shows that the development of the science was accomplished by a justified introduction and improvement of cytogenetical techniques and methods. The results obtained were applied and helped in the understanding of philogenic relationships, evolution of species, biomonitoring as a method of conservation of authentic genofounds of species and the ecosystem. Cytogenetic achievements were used in the diagnostics of aberrant genetical forms and in the prevention of their distribution throughout the population. The development of information system has given rise to a new approach in Cytogenetic research and at least 10 megaprogram systems have been developed for detailed cariotype analyses. The introduction of new methodology and technics could secure precise cytogenetical analyses, detailed gene mapping and a fruitful application of genetic advances in biotechnology and embryotechnology.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
A CYTOGENETIC STUDY OF INTERSPECIFIC SUNFLOWER HYBRIDS (H. annum L. x H. occidentalis Riddel)
Jovanka ATLAGIĆ
Institut za ratarstvo i povrtarstvo, Novi Sad
H. occidentalis is a diploid, perennial sunflower species whose phylogenetic origin and genetic relationship has not been as adequately as studied other species from the genus Helianthus. The objective of this paper was to study the possibility of crossing H. occindentalis with cultivated sunflower. The meiosis of the species and the HF1 hybrid was analyzed by the aceto-carmine method (Georgieva, 1976) and pollen vitality by the method of coloring (Alexander, 1969). Three hybrid combinations were obtained from the cross between cultivated sunflower and H. occidentalis. A low percentage of irregularities (fast and lagging chromosomes) occurred during the meiosis of H. occidentalis. Chromosome bridges and fragments were not detected. Chromosome pairing in diakinesis was normal (17 bivalents). High pollen vitality (90.81-97.68%) was observed. In the HF1 interspecific hybrids, several irregularities occurred during meiosis. The average number of bivalents per meiocile was 13.39. The percentage of meiocites with bivalents was 82.35%. Besides bivalents, univalents (9.19% of meiocites) and multivalents (8.46% of meiocites) were also detected in diakinesis. Fast chromosomes in metaphase I, lagging chromosomes in anaphase I and telophase II were detected in a high percentage of meiocites. Chromosome bridges and fragments were detected in 10.52% of meiocites. Pollen vitality in the HF1 hybrid was low (3.72-52.94%). The results obtained show that, although these are species with an identical number of chromosomes, differences exist in the chromosome structure. This indicates that the genome of diploid, perennial species differs from the genome of annual species. The question remains open whether the basic number of chromosomes (17) in the sunflower represents a single genome or several genome.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
THE DISTRIBUTION OF CONSTITUTIVE HETEROCHROMATIN AND NUCLEOLUS ORGANIZERS IN LIZARDS OF THE FAMILY LACERTIDAE (SAURIAJ)
Jelena BLAGOJEVIĆ i VUJOŠEVIĆ M.
Odeljenje za genetiku, Institut za biološka istraživanja "Siniša Stanković", Beograd
The family Lacertidae is characterized by karyotipic uniformity. Almost all species have the same number and morphology of chromosomes, 36 acrocentric macrochromosomes and 2 microchromosomes. The structure of chromosomes of eight species from the family Lacertidae (Podarcis sicula, P.muralis, Lacerta viridis, L. trilineala, L. meliscllensis, L. taurica, L. agilis and Alroides nigropunctatus) was analyzed regarding the distribution and amount of constitutive hetcrochromatin, and the presence and localization of nucleolus organizers. Interspecies variability in the number and position of nucleolus organizers was detected. The presence of constitutive heterochromatin in the centromeric region was the same for all species while terminal and interstitial localization of constitutive heterochromatin characterized only some of them. Phylogenetic relationships between species in the light of results obtained were considered.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
THE INFLUENCE OF OXYTOCIN ON MITOTIC ACTIVITY AND ACCURRENCE OF CHROMOSOMAL ABERRATIONS IN HUMAN PERIPHERAL BLOOD LIMPHOCYTE CULTURES
N. ĐELIĆ 1, B. SOLDATOVIĆ
1 i M. ANĐELKOVIĆ 2
1 Katedra za biologiju, Veterinarski
fakultet, Beograd
2 Katedra za genetiku, Biološki fakultet PMF, Beograd
The influence of oxytocin (SyntocinonR) on the occurrence of structural and numerical chromosomal aberrations was examined in cultures of human peripheral blood. The limphocyte cultures were treated with four concentrations of oxytocin (0.01 IU/ml, 0.02 IU/ml, 0.05 IU/ml and 0.1 IU/ml) and a negative as well as a positive control was included. The occurrence of chromatid and chromosomal breaks and gaps was evaluated among structural aberrations, and the occurrence of heteroploid and polyploid cells was deceted as measure of numerical aberrations. Within the range of the tested concentrations, oxytocin did not induce significant alterations in the occurrence of structural and numerical aberrations, compared to the negative control level. The level of structural aberrations in the positive control (10 MNNG) was significantly raised, whereas the level of numerical mutations was not significantly changed. In order to evaluate some possible cytotoxic effects, a mitotic index was established for each experimental concentration, negative and positive controls. No statistically significant alterations of the mitotic index values were observed in relation to the control level. On the basis of the results obtained oxytocin did not show genotoxic effects in the tested concentrations.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
THE FREQUENCY OF THE GAP AND BREAK TYPE OF STRUCTURAL CHROMOSOME CHANGES IN INDIVIDUALS OF THE FISH SPECIES STIZOSTEDION VOLGENSE /PERCIDAE/, CAUGHT AT SOME LOCALITIES OF THE RIVER DANUBE
Svetlana FIŠTER i B. SOLDATOVIĆ
Veterinarski fakultet, Beograd
The karyotype of Slizostedion volgense / Pisces, Percidael is presented, together with the number and morphology of chromosomes. The frequency of structural chromosome changes of gap- and break types in individuals caught at four localities of the river Danube: Beška, Zemun, Višnjica and Grocka, was analyzed. The highest mean values of gap- and break types of chromosomal changes were established in fishes from localities m the Danube that were placed downstream from the mounth of the river Sava at Belgrade: at Višnjica and Grocka. The results obtained revealed the presence of genotoxical agents in some of investigated localities. The water of the Danube at the cited localities could be marked as a point of periodic or permanent hazard from a genotoxical point of view.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
DIFFERENT RESPONSES TO GENOTOXIC AGENTS DEPENDING ON THE GENETIC CONSTITUTION
Drinka MERĆEP 1, A. ĐUJIĆ 2, Z. MAGIĆ 2 i M. SIMOVIĆ 2
1 Veterinarski fakultet, Beograd
2 Institut za medicinska istraživanja VMA, Beograd
Ionizing radiation as an undoubtedly genotoxic agent is suitable for comparing responses in different strains of the same animal species. The most competent biological indicator for radiation damage is the presence of dicentric and "ring" chromosomes, wich arc a confident sign that the organism was irradiated. The BALB/C mouse strain is very sensitive, while C57BL/6 is more resistant to ionizing radiation measured with LD50/30. This phenomenon could be explained by genetic factors controlling special physiological functions, which suppose the existence of genetic predispositions for resistance to radiation trauma. The experiment was performed on BALB/C mice which are radiosensitive and whose LD50/30 is 5.98 Gy, and strain C57BL/6 which is more resistant and whose LD50/30 is 8.308 Gy. Animals were sacrificed at different intervals after irradiation: on the first, the fourth and the tenth day. Six animals for each term and from each strain were used. A classical cytogenetic method was used to obtain chromosomes. The results obtained support the fact that an important element in the response of an organism is its genetic constitution.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
THE EFFECT COLCHICINE ON SOME QUANTITATIVE CHARACTERS OF SUGAR BEET
Snežana MEZEI, L. KOVAČEVIĆ i N. ČAČIĆ
Institut za ratarstvo i povrtarstvo, Novi Sad
Triploid sugar beet hybrids are produced by converting diploid genotypes to a tetraploid level. Induced polyploids represent a source of new genetic variability. In sugar beet breeding, multigerm tetraploids obtained by the action of colchicine were used as pollinators for sterile diploids, and monogerm tetraploids as maternal components for diploid and tetraploid multigerm populations. The induction of autotetraploids is based on the mutagenic effect of colchicine on spindule fibres. The function of the colchicine effect on apical mcristcmcs is to convert a high percent of the original genotype to the tetraploid level. Since not all cells were in the phase of active mitotic division, a certain percentage of original genotypes remained at the diploid level. The aim of this study was to compare original diploids and diploids which were under treatment with colchicine but did not change their ploidy level; to determine whether colchicine affected only the spindle fibres or the complex of genes which carry different quantitative characters (root yield, sugar content, yield of polarized and white sugar). An aqueons solution of colchicine was applied to the apical points of growth of the genotypes studied under greenhouse conditions. Cytological analysis was performed according to the aceto-carmine squash method. Sugar beet roots with 36 and 18 chromosomes were analyzed the following year and all plants with mixoploid tissues were removed from the trial. The results of the study showed that differences exist between the genotypes studied and between the original diploid populations, the diploid population which was not transformed to the tetraploid level and the induced autotetraploids with respect to the yield of root and white sugar. Regarding the other quantitative characters studied, the sugar content, K, Na, alpha-N and yield of polarisation sugar, the differences established were not statistically significant.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
A METHOD FOR MAKING NUMERICAL KARYOTYPE ETALONS
B.P. PAVLOVIĆ
Institut za šumarstvo, Beograd
The quantity of chromosome material joined to the centromere is called the "centromere load" (CO). The chromosome length is proposed as a possible measure for intrakarytype comparison of centromere loads. The base for calculating centromere load indices is the arithmetic mean of chromosome lenghts of the karyotype (specific centromere load - SCO). These index values can be used in comparisons of Karyotypes. A mathematical; statistical method has been developed for karyotype analysis. A complex of measured lenghts, calculated single analytical and composed indices that presents a quantity state of one typical chromosome set (karyotype), is called a numerical karyotype ctalon. In addition three subsets of index values are calculated on the basis of means of arms (long, short, or, long and short) lengths. These data subsets represent simulated karyotypes that originate from deletions of all short, or long arms, as well as by centric fissions of all chromosomes. Symbols and formulations for measuring and calculating quantities are given in an algebraic table form of the numeric karyotype etalon.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
NUMERICAL KARYOTYPE ETALONS Erinaceus europaeus europaeus
B.P. PAVLOVIĆ
Institut za šumarstvo, Beograd
The chromosomes of the published male and female karyotypes of ssp. Erinaceus europaeus europaeus (Folio 52 in An atlas of mammalian chromosomes, eds. Hsu, Benirschcke 1968) have been measured. The data were used for karyometric analysis that is presented in two table sets - numeric karyotype etalons. The ratio of lengths (W) of the longest chromosome to the shortest one in the male karyotype (W=8.4194) was smaller then in the female karyotype (W=9.7079). The indices of centromere loads (H) in the male karyotype decreased from H1 = 1,7988 to H48 = 0,2136 (Y-chromosome was not the shortest one), and in the female karyotype from H1 = 1,8744 to H48 = 0,1931. Unit index values (H=l) were located between H24 and H25 (male), or H26 and H27 (female). Long arm karyotype proportions were PL = 0.6527 (male) and PL = 0.6506 (female). Correlations of sister chromatid lengths in the karyotype were high, male and female: r = 0.9S7 and ρ = 0.986 respectively. The components of H-index variations (male sH =0,577 and female sH =0,573) were higher than for the long arms on Lindices (male sL =0,438 and female sL =0,426), or for the short arms (K-indices) in the karyotypc, (male sK =0,262 and female sK =0,255). Simulations of deletions of all short arms, or long arms, resulted in increased values of the corresponding components of variation similary to simulations of centric fissions of all chromosomes.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
THE RELATION OF VARIATION COMPONENTS INSIDE THE KARYOTYPE OF MAMMALIA AND PISCES: INDICES OF PARTIAL CENTROMERE ARM LOADS AND INDICES OF CENTROMERE LOADS
B. PAVLOVIĆ, i Nevenka PAVLOVIĆ
Prirodno-matematički fakultet, Sarajevo
Each unpublished numeric etalon for the 270 mammalian and piscian karyotypes (B. Pavlović) consists of a value for the relation (PsA=sA/sH) between the chromosome arm variation and the chromosome variation given in units of mean chromosome length for each karyotype. The parameters (PSA) have been compared among taxa and related to 2n value of the karyotypes. The arithmetic mean value of PSA is 1.005, or 1.434 for piscian, and 0.939 for mammalian karyotypes. The parameters ranged from 0.530 to 2.985, or 0.636 to 2.985 (Pisces), and 0.530 to 2.183 (Mammalia). There were several values of 2n with wide ranges of the relation PSA. Specific relations of 2n, PSA have been shown for the represented mammalian orders.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
THE CHROMOSOME COMPLEMENTS OF SOME FISH SPECIES FROM THE CYPRINIDAE FAMILY
Slavica SIMOVIĆ i G. MARKOVIĆ
Agronomski fakultet, Čačak
In this paper the results from a study of the chromosome complements of five fish species from the Cyprinidae family are presented. These species are common in the waters of the Danube basin. Our studies showed that diploid chromosome sets of the analyzed species consisted of 50 chromosomes (2n=50) as follows: Chondrostoma nasus 15 metacentrics, 15 submctacentrics, 12 subtelocentrics and 8 telocentrics; Alburnoides bipunctatus - 8 pairs of metacentrics, 7 pairs of submetacentrics, 6 pairs of subtelocentrics and 4 pairs of telocentrics; Blicca bjoerkna -6 pairs of metacentrics, 7 pairs of submctacentrics, 6 pairs of subtelocentrics and 6 pairs of metacentrics; Abramis sapa - 12 metacentrics, 14 submetacentrics, 10 subtelocentrics and 14 telocentrics and Abramis ballems - 15 metacentrics, 10 submctacentrics, 12 subtelocentrics and 13 telocentric chromosomes when compared with data from the literature. Certains differences regarding the morphological types of chromosome of certain fish species were established.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
A LARGE ACROCENTRIC CHROMOSOME IN THE FIRST PAIR OF AUTOSOMES IN NATURAL POPULATIONS OF Mus musculus, Linne 1758
Z. STANIMIROVIĆ 1, Mirjana
VUČINIĆ 2, B. SOLDATOVIĆ E. HERZOG 1
1 Veterinarski fakultet, Beograd
2 Poljoprivredni fakultet, Beograd - Zemun
In this study, polymorphism of chromosomes in natural populations of Mus musculus was examined. The investigations were conducted in 5 different localities (Sevarice, Glušci, Lipolist, Bogatić, Sremska Mitrovica) in North-West Serbia, around the Sava and Danube. Besides the frequent finding of a Robertsonian type of chromosomal polymorphism, the presence of one large acrocentric chromosome in the first pair of autosomes, unusual for the mouse karyotype, was observed in a large number of investigated animals. By the application of appropriate cytogenetical methods, it was established that the altered chromosome had two additional insertions, located in ICs and IE regions. Based on the results obtained from the karyotype tests in mice and data from the literature, it can be concluded that the observed acrocentric chromosome resulted as an adaptation of mice to an increased quantity of environmental, agrochemical and industrial pollutants in the localities investigated.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
CYTOGENETIC CHARACTERISTICS OF PLANTLETS REGENERATED BY IN VITRO WHEAT (Triticum aestivum L.) ANTHER CULTURE
S. ŠESEK
Institut za ratarstvo i povrtarstvo, Novi Sad
Cytogenctic analysis of 189 green plantlets regenerated by in vitro Triticum aestivum L. anther culture was performed. A haploid number of chromosomes (2n=3X=21) was found in 49.2% of the studied plantlets, while 41.3% of plantlets had a diploid number of chromosomes (2n=6X=42) in somatic cells. This means that 90.5% of green plantlets were either polyhaploids or hexaploids. Haploaneuploids, i.e. polyhaploids with 2n=19, 20 or 23 chromosomes, occurred with the frequency of 2.1%, while the frequency of diploaneuploids (hexaploids with 2n=40, 41, 43 or 44 chromosomes) was 4.2%. Only 2 polyploids were found: one with 2n=8X=56 and one with 2n=9X=63 chromosomes, which was 1.1% of the total number of analyzed plantlets. There was 2.1% of mixoploids, i.e., of plantlets with cells with a haploid and diploid number of chromosomes.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
CHROMOSOMES OF FROGS FROM THE FAMILY RANIDAE (ANURA, AMPHIBIA) -A COMPARATIVE ANALYSIS
N. TANIĆ, Olivera SPASIĆ, Jelena BLAGOJEVIĆ i M. VUJOŠEVIĆ
Odeljenje za genetiku, Institut za biološka istraživanja "Siniša Stanković", Beograd
Chromosomes of a few species of the genus Rana (Anura, Amphibia) from different localities have been compared concerning the number and position of the nucleoli organizer region (NOR), as well as the amount and distribution of constitutive heterochromatin regions. All species had the same number of chromosomes (2n=26), but there were differences in their morphology. Polymorphism in the number and position of NORs in chromosomes has been found. It was shown that polymorphism in the distribution and amount of C-bands also existed. In spite of the variability of these chromosome characteristics in the genus Rana, we can generally say that constitutive heterochromatin is most commonly present in centromeric and telomeric regions, and rarely in the interstitial region. In most species NOR is localized in a single pair of small submetacentric chromosomes.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
"ROSOL", AS AN EVENTUAL INDUCOR OF POLYPLOID TYPE CHROMOSOME ABERRATIONS
Marijana VUČINIĆ 1, Z. STANIMIROVIĆ 2, B. SOLDATOVIĆ 2
1 Poljoprivredni fakultet, Beograd - Zemun
2 Veterinarski fakultet, Beograd
Rosol (VZ-Zemun) is an anticoagulant rodenticide, with sodium-warfarin as the active substance. The appearance of cytogenetical changes in meiotic testicular cells of experimental mice, during a seven day treatment with "Rosol" was studied. The substance was administered by gastric tube in a dose of 0.13 mg per kilogramme of body weight. Cytogenetical tests revealed an induction of structural and numerical aberrations of the polyploid type in cells of the germinative epithelium. The results obtained supply additional information on the effects of anticoagulant rodenticides and to explain the appearance of resistant strain of rodents.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
INTERPOPULATION POLYMORPHISM OF B-CHROMOSOMES IN YELLOW NECKED WOOD MICEAPODEMUS FLAVICOLLIS (MAMMALIA, RODENTIA)
M. VUJOŠEVIĆ i Jelena BLAGOJEVIĆ
Odeljenje za genetiku, Institut za biološka istraživanja "Siniša Stanković", Beograd
Populations of yellow necked wood mice (Apodemus flevicollis) are often characterized by the presence of B-chromosomes. Samples from nine localities (total of 669 animals) were compared for the presence of Bs. In all samples, except one, Bs were present, but in significantly different frequencies (0.11-0.64). It was found that the difference in the frequency of animals with Bs was dependent on the type of environment, altitude and the sex. Significant differences in the frequency of animals with Bs were associated with the sampling time, and the age category.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
CHROMOSOME ABERRATIONS FOUND BY CORDOCENTESIS
Slavenka ADŽIĆ 1, Stefanija MARKOVIĆ 2, D. PLEĆAŠ 2 N. RADUNOVIĆ 2 i Z. PILIĆ 2
1 Institut za ginekologiju i akušerstvo, Beograd
2 Institut za ginekologiju i akušerstvo KCS, Beograd
Along with other methods, in the Institute of Gynecology and Obstetrics, cordocentesis is used. The indications arc: the age of pregnant women (36-47), foetus malformations, a slowing down if intrauterine development, oligoamnion, hydroamnion, etc., which are detected by ultrasound at regular check ups. In the group of older pregnant women (173), 21 (12.14%) chromosome aberrations were found. In 88 pregnancies with disordes discovered by ultrasound 13 (14.77%) chromosome aberrations were detected. In all cases of abnormal karyotype termination of the pregnancies was performed.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
IN VITRO GENOTOXICOLOGICAL CHARACTERISATION OF IVERMECTIN AND PRAZIQUANTEL
Nevenka ALEKSIĆ
Veterinarski fakultet, Beograd
The effects of the antiparasits ivermectin and praziquantel in PHA-stimulated human lymphocyte cultures were observed. Genetic effects were evaluated by the determination of sister-chromatid exchange frequencies (SCE). Both drugs proved to be genotoxic in certain concentrations in our test system. Ivermectin was tested in the concentrations 0.15, 0.30, 0.60, 1.20 and 2.40 ug/ml of medium. Concentrations higher than 0.15/ug/ml induced a statistically significant increase of SCE. Praziquantel was added in the doses of 2.84, 5.68, 11.36, 22.72 and 45.44 ug/ml of medium. The drug statistically increased the SCE frequency in the concentrations of 2.84, 5.68 i 11.36 ug/ml; in higher doses it produced mitotic arrest.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
A CEPHALOMETRIC ANALYSIS OF PATIENTS WITH KLINEFELTER'S SYNDROME
M. BABIĆ i M. MICIĆ
Institut za biologiju i humanu genetiku, Stomatološki fakultet, Beograd
Sex chromosomal aneuploidy has an influence on cranial growth and the shape and position of craniofacial structures. Twenty-eight adult males with Klinefelter's syndrome were analysed and compared with a control group. Linear and angular measurements were taken from the lateral cephalograms of all subjects under the same conditions. Reduced cranial growth and a specific craniofacial morphology in men with an extra X chromosome was recorded. The influence of the extra X chromosome on cranial base growth, facial height, maxilla, mandible and intermaxillary relations were analysed. The effects of sex chromosomes imbalance on craniofacial structures was discussed.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
THE DEGREE OF GENETIC HOMOZIGOSITY IN A SAMPLE OF PSYCHIATRIC CLINIC PATIENTS AND IN HEALTHYREPRESENTATIVES OF PRIŠTINA INHABITANTS
S. BRANKOVIĆ i G. SAVIĆ
Prirodno-matematički fakultet, Priština
The presence of homozygously recessive characters ("HRC-TEST') has been verified for 29 qualitative morphophysiological systems, and compared in a sample of /A/ 140 patients from the Psychiatric Clinic in Priština, and /B/140 healthy individuals as a control sample. The average presence of HRC's was found to be significantly greater in the A-group, amounting to 8,8 (i.e. cca. 30%), whereas it was much lower among healthy individuals, both of Albanian (6,2) and of Serbian (6,5) nationality (i.e. 22% of studied traits). The frequency distribution of the analysed characteristics was also significantly different, suggesting a population-genetic difference between samples A and B. This difference was especially emphasized in 8/29 of the traits studied.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
THE DEGREE OF GENETIC HOMOZYGOSITY AMONG PATIENTS WITH CONGENITAL HIP DISLOCATION AND A CONTROL SAMPLE CONSISTING OF SCHOOL CHILDREN FROM BELGRADE
Suzana CVJETIČANIN i D. MARINKOVIĆ
Biološki fakultet, Beograd
Assuming that congenital dislocation of the hip (CDH) is a genetically controlled disease, we made a hypothesis that an increased homozygosity level, as well as decreased variability in the patients, could be a population-genetic parameter for the prediction of the illness. Taking into consideration the experience of numerous scientists who studied the nature of the inheritance of mono- and oligogenically controlled qualitative traits, we applied a methodology to estimate the proportion of such homozygosly recessive characters ("HRC-TEST") in samples of diseased and healthy/control individuals. After analysis of 21 HRC's, it was found that 17 of them were significantly more frequent among 93 children from Belgrade. The average number of HRC's in the two samples was 7.1 + 1 and 5.2 + .1, respectively. The described methodology can be used for further analyses, with the hope that it can be applied as an early prognosis for decreased resistance to different diseases.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
A HIGH INCIDENCE OF H-ras GENE POINT MUTATIONS IN TWO TYPES OF HEAD AND NECK NEOPLASIAS
N. DEDOVIĆ 1, J. MILAŠIN 2, V. PETROVIĆ 3, G. STAMENKOVIĆ 1, M.
GAVRIĆ 3, Z. NIKOLIĆ 3
1 Institut za nuklearne nauke, Vinca
2 Institut za biologiju i humanu genetiku, Stomatološki fakultet
3 Klinika za oralnu i maksilofacijalnu hirurgiju, Stomatološki fakultet, Beograd
The DNA from 17 specimens of pleomorphic adenomas of the salivary glands (PA) and 9 specimens of squamos cell carcinomas of the lip (SCC) has been screened for the presence of activating mutations in the H-ras gene (codon 12,13 and 61), known to be involved in the pathogenesis of various human neoplasias. Using a sensitive technique of hybridization with synthetic oligonucleotide probes on an in vitro amplified tumor DNA, point mutations, mainly in codon 12 of the H-ras gene, were detected in 6 specimens of PA (35%) and 5 specimens of SCC (55%). G->C and G->T transversions at the first position of codon 12 were the predominant base substitutions in PA and SCC, respectively. This high incidence of mutated ras genes suggests that their alteration may play an important role in the pathogenesis of some head and neck neoplasias, benign as well as malignant.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
THE ROLE OF GENETIC FACTORS IN THE ETIOPATHOGENESIS OF CARDIOVASCULAR DISORDERS
Vukosava DIKLIĆ 1, Marija KOSANOVIĆ 1, Milica TODORIĆ 2, M. VUKOTIĆ 2, S.P. STOJKOVIĆ 2
1 Institut za biologiju i humanu genetiku
Medicinskog fakulteta Univerziteta u Beogradu, Beograd
2 Dom zdravlja Rakovica, Beograd SM
Our genetically oriented study is a part of the Rakovica study of CVD (RASCO). Having in the mind that the appearance of early CVD is grouped in families in a way that strongly indicates the importance of genetic factors, our intention was to identify high risk individuals and families for preventive measures. A study was undertaken of 176 probands (69 men and 107 women) who had some of risk factors of CVD or CVD itself before the age of 55 (men) and 65 (women). The control group consisted of 45 young and, healthy men and 28 women. Family grouping of CVD risk factors and CVD was investigated among 944 relatives of probands and 450 relatives of control subjects. The prevalence of the CVD risk factors: hipertension, dyslipidaemia, diabetes mellitus and CVD in relatives of probands was significantly higher (p< 0.001) than in relatives of controls. A positive correlation between the clinical phenotype of probands and the prevalence of CVD risk factors and CVD in their relatives was evident. Male and female probands, with hypertension as a single risk factor, had 43.3% and 46.0% relatives with CVD. In probands who had two or more CVD risk factors, or risk factors combined with CVD, this percentage was higher, 50.9% and 54.0% respectively. The mortality of CVD risk factors or CVD was significantly higher (p<0.005) in relatives of probands than in relatives of controls. Our study confirms the general agreement that genetic factors are operative in CVD as well as in the three major risk factors for the development of CVD.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
CYTOGENETIC ANALYSES REVEAL "ATYPICAL CELLS" IN THE PERITONEAL DIALYSIS EFFLUENT
Nada DIMKOVIĆ, Vitana KOSTIĆ, Dragan JOVANOVIĆ i Nada BARJAKTAROVIĆ
Kliničko-bolnički centar "Zvezdara", Beograd
Cytogenetic analyses were performed on dividing cells from the peritoneal effluent of 26 patients (pts) undergoing chronic peritoneal dialysis (CPD). Numerical and structural abnormalities of the karytype served as diagnostic criteria for "atypical cells". The following cytogenetic abnormalities were observed in 7 patients: hyperdiploidy (in 6 pts), hypodiploidy (in 2 pts) and marker chromosomes (in 2 pts). In three patients more than one chromosome abnormality was present. Dividing cells with normal mitoses were observed in 11 patients while in the remaining eight patients no dividing cells could be found. There were no differences in age, sex, duration of dialyses and the incidence of peritonitis between patients with pathological mitoses and those without them. The question of whether this unexpected finding is a consequence of an immunosuppressed uremic status, dialysis procedure or some other factor(s) remains to be elucidated.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
HISTOLOGIC FINDINGS IN THE OVARY OF A TURNER'S SYNDROME PATIENT
B. ĐORĐEVIĆ, D. MIHAILOVIĆ, D. STOJANOVIĆ, C. KUTLEŠIĆ, M. ČEKEREVAC, Z. POPOVIĆ i Z. STANKOVIĆ
Institut za patologiju, UKC, Niš
The 45,XO karytypc is characterized by Turner's syndrome. Typical clinical features of 45,XO Turner's syndrome arc short stature, primary hypogonadism, infantile genitalia, pterygium collu, cubitus valgus, a shield-like chest with widely spaced nipples, lymphedema and a variety of congenital malformations such as a horseshoe kidney and coaretation of the aorta. In this paper, histologic finding in the ovary of a 16-year-old girl with Turner's syndrome arc presented. Histologically, the ovary consisted only of the stroma and structures which are normally found in the ovarian hilus: rete tubules and clumps of cells that closely resamble Leydig cells. Follicles are totally absent.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
A REPORT OF A 9p/10p FAMILY TRANSLOCATION
Slobodanka GRKOVIĆ i Vesna IVANOVIĆ-DERETIĆ
Dečja klinika Medicinskog fakulteta, Beograd
This report describes a family in which two members of the same generation, both phcnotypically normal, had a balanced translocation carrier 9p/10p. In this family four children were malformed. One of them had an unbalanced karytype: 46,XY,-9 +der/9/t/9:10/p22;q24/pat. In this report we have discussed the clinical manifestation of partial monosomy 9p- and partial trisomy 10p+. Prenatal diagnosis was done in the next two pregnancies. Healthy children were borrn.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
GENETIC DETERMINANTS IN THE PROGNOSIS OF NEUROBLASTOMA
Marija GUĆ-ŠĆEKIĆ, Dragana ANĐELKOVIĆ, V. JURUKOVSKI i Gordana PILIĆ-RADIVOJEVIĆ
Institut za zdravstvenu zaštitu majke i deteta RS, Beograd.
Neuroblastomas are characterized by distinct genetic features. Human neuroblastoma cells often carry cytogenetic abnormalities signalling an amplification of the gene N-myc. In most cell lines amplified N-myc is localized in homogeneously staining regions (HRSs) of chromosomes and on double minute chromosomes (DMs). Neuroblastomas arc genetically complex and there are at least two distinct loci for the putative suprcssor gene (Ip and 14q) that are deleted independently. Both of them are associated with advanced stages of the disease. 40 NB patients were subjected to cytogenetic analysis and 2 of them were analysed both aytogenetically and by fluorescent in situ hybridization for the presence of the amplified N-myc gene. In 19 cases cytogenetic analysis revealed normal karyotypes. Near-tetraploid cells were found in mosaic with normal cells in 14 cases and were correlated with advanced stage of the disease. HSRs were present in karyotypes of 4 patients and were correlated with a poor prognosis. Deletion or loss of hetcrozygosity (LOH) for the short arms of chromosomes 1 and 11 in mosaic with normal cells or near-tetraploid cells were found in 2 patients in stages III and IV. The cytogenetical analysis in one case with an advanced stage of disease revealed LOH for Ip and 3p. FISH performed in two patients with HSRs showed the presence of the amplified N-myc gene.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
A REPORT OF AUTOSOMAL WHOLE ARM TRANSLOCATION
Vesna IVANOVIĆ-DERETIĆ, Slobodanka GRKOVIĆ i B. GARŽIČlĆ
Dečja klinika Medicinskog fakulteta, Beograd
This report deals with the possibility of the identification, segregation and transmitting of an autosomal whole arm translocation based on a case of 46,XY, t/2p5p;2q5q/. Two pregnancies were miscarried. In the third pregnancy an unbalanced karyotype was found. In the fourth pregnancy prenatal diagnostic was done and the karyotype was balanced: 46,XY, t/2p5p;2q5q/.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
A POPULATION GENETICIST IN (PRE) CLINICAL PRACTICE CONCERNING CHRONIC WIDE-SPREAD NONCONTAGIOUS DISEASES
Zorica JANAKOVA
Dom zdravlja, Jagodina
This review presents the work of our medical workers (population geneticist, doctor-assistant, nurse and laboratory technician) that is based on continuous control of chronic wide-spread noncontagious diseases. It is based on, sistematical, primary and rational prediction and prevention of: allergic diseases particularly bronchial asthma in childhood, diabetes and related diseases, and other diseases with polygene multifactorial etiology. Besides laboratory tests and consultations. This center also includes a population-genetic, laboratory and (pre)clinical educational treatment of the individual and families with hereditary liabilities and a genetical high-risk factor. Population-genetictreatment includes the selection of individuals (families) with established genetical and environmental factors of risk. Selection is systematically performed among pregnant women and women who have just given birth and relatives of patients, who arc, in endangered business firms, schools and other groups and also at high-risk ages in order to diagnose the susceptibility. The first person (family) who is selected significantly rationalised further laboratory treatment, even if it concerns a high degree of atopy, immunological defects, homozygote elevated genotype, or other aspect of family susceptibility to diseases with allergic reactions, or to the early stages of diabetes. Such an approach to this massive pathology, makes it possible that early stages become available for further (pre)clinical-educational treatment and continuous monitoring aimed at prediction or dela of the clinical manifestation.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
THE SIGNIFICANCE OF PRELIMINARY
SELECTION OF PERSONS ATRISK IN SYSTEMATIC CHECKUPS FOR THE DETECTION OF EARLY
STAGES OF DIABETES MELLITUS
Zorica JANAKOVA
Dom zdravlja, Jagodina
From the aspect of population genetics and medical practice, genetical, constitutional, physiological and environmental parameters for the most effective selection of individuals with a decreased tolerance to glucose (DGT) neve been established in order to diagnose liability to diabetes mellitus we also wish to emphasize the high efficiency and economy of the suggested method of systematic checkups for the detection of early stages of diabetes mellitus which included preliminary selection of individuals for OGTT. For example every third person (adult) should take the OGT-test. As a result, 40% of the tested persons will be found to decreased tolerance to glucose. Moreover, only every seventh woman who has just given birth or pregnant woman would need to be tested in order to detect gestational diabetes in one third of the tested women. Using the experience of the genetic counselling office for small groups of medical workers in public medical centres in order to search for latent stages of diabetes, to prevent its clinical manifestation, and to suppress other wide-spread noncontagious diseases.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
HUMORAL FACTORS IN THE VARIABILITY OF ALLERGIC REACTIVITY
Zorica JANAKOVA
Dom zdravlja,
Jagodina
A group of 300 children with allergic reactivity (AR) have been observed clinically, genetically and in the laboratory (IgE, IgA, IgM, IgG, IgD, alfalantitrypsin, transferrin, haptoglobin, coeruloplasmin, alfa2-macroglobin i C3-and C4-complement). In the genetic counselling unit for chronic common noninfectious diseases. Increases in the degree of atopy and severity of allergic diseases were not followed by continued changes of the frequency of pathological levels of serum immunoglobulins, plasma-proteins and components of complement, but with a high degree of heterogenezity and individual variability. Humoral imunodeficiency and amplified chronic nonallergic inflammation take part in the appearance of AR with moderate severity and with a moderate or normal degree of atopy. High AR and it severest clinical forms are etiopathogenitically relatively autonomous and primarily depend on the high degree of atopy. A moderately high AR is more frequently followed with extreme deviations of the lavels of the plasma proteins and components of complement, or there are moderate deviations from normal values. There are two exceptions: alfal-antitrypsin (extremely low values are characteristic for the highest level of AR) and hepatpglobin (increased and decreased values often follow high AR). The form of the variability of humoral factors follows the other forms of genetic and environmental variability of AR.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
CHROMOSOME ABERRATION AND THE CB MIKRONUCLEI TEST AS COMPARATIVE METHODS FOR BIOLOGICAL DOSIMETRY OF IONISING RADIATION
Gordana JOKŠIĆ
Klinički centar Srbije Institut za medicinu rada i radiološku zaštitu "Dr Dragomir Karajović", Beograd
The cytochalasin B micronuclei test as a new method in biological dosimetry is based on the fact that many chromosomal aberrations (dicentric chromosome, ring chromosome, excess eccentrics, chromosome breaks, incomplete chromatid type of aberrations, triradials and isochromatid deletions) pose problems during anaphase resulting in the formation of micronuclei. There is an almost ideal congruity of results on the chromosome aberration frequency and the CB micronuclei test, in the aberration yield after acute irradiation. For low X and gamma irradiation doses (0.1-05 Gy), the calibration curve is linear in character (defined by the mathematical expression y=c+aD. The lowest dose hat could be determined by this method for X-irradiation is 0.1 Gy, and for gamma irradiation 0.15 Gy. For irradiation doses of 0.1-4 Gy, X and gamma calibration curves have a linear-quadratic shape defined by the mathematical expression y=c+aD+/3D . The highest irradiation dose which can be reliably determined is 4 Gy. The CB micronucleus test has an advantage as a method because from one sample several thousand lymphoblasts can be analysed and therefore a more objective pattern of radiation damage from non-uniform irradiation and internal contamination with radionuclides can be obtained. The specificity of dicentric chromosomes as chromosome aberration representing the most reliable indicator that has been irradiated a cell should be further retained as an irreplaceable biodosimetric parameter in accidental overexposures and occupational pathology.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
A FREQUENCY ANALYSIS OF GENETICALLY CONTROLED QUALITATIVE CHARACTERISTICS AMONG WOMEN WITH BREAST CANCER IN THE HIGH RISK GROUP AND CONTROL INDIVIDUALS
Dragana JOVANOVIĆ i D. MARINKOVIĆ
Biološki fakultet univerziteta u Beogradu, Beograd
A study of patients with a family syndrome (PFS=26), patients without a family syndrome (PWFS=44), a high risk group (HR=70) and a control sample (C=70') has shown the existence of population - genetic differences in the presence of 10 selected homozygously recessive characteristics (HRC-test) and observed dermatoglyphic characteristics. We found a statistically significant difference between the PWFS and C sample, also between the PWFS and HR group for two traits. PFS and HRC samples showed in comparison with the C group a significant difference for only one characteristic. We also detected a significant difference in the number (0-6/6) of HRC's between the PWFS and C group, as well as between the PWFS and HR sample. The comparison between PFS and control values was almost statistically significant. PWFS on the average had a decreased number of HRC s (1.9) similar to PFS (2.0), while the HR group had on average 2.4 HRC s, and for the C sample the average homozigosity was detected as 2.7. A dermatoglyphic study also showed a difference between the studied groups. We noticed a difference in the frequency distribution of 28 observed dermatoglyphic patterns between PFS and the control, also between PWFS and the control sample. Three dermatoglyphic patterns did not appear in group C, but they were present among patients and the HR sample with a frequency of about 3%. The percent of individuals containing six or more whorls was highest in the PWFS (16%), next in the HR group (14%} and in PFS (11%), and lowest in the control sample (7%). Comparison of ABO and Rh blood group frequencies among the four samples did not show statistical significance.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
EUROCAT IN VOJVODINA
J. JOVANOVIĆ-PRIVRODSKI, A. KRSTIĆ, F. POPIĆ-PALJIĆ, R. ALEKSIĆ i G. RACA
Medicinski fakultet, Institut za zdravstvenu zaštitu dece i omladine, Novi Sad
Today the European standard for registration of congenital anomalies is EUROCAT wich starlet to be used 1979 in West Europe. EUROCAT registration was used in Vojvodina in 1992. Of 15,025 newborns 404 malformed cases were found wich gave an incidence of 2.68%.Thc EUROCAT register shows:
1) epidemiological data and the geographical distribution of malformed persons,
2) environmental agents which give malformations (level of teratogenesis) and mutations (level of mutagenisis)
3) the malformed newborns who need treatment
4) the measures for a preventive program
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
THE HLA SYSTEM - A GENETIC MARKER FOR PRENATAL DIAGNOSIS OF INUSLIN DEPENDENT DIABETES MELLITUS (IDDM) - Case report
J. JOVANOVIĆ-PRIVRODSKI, A. KRSTIĆ, F. POPIĆ-PALJIĆ, Z. PILIĆ
Medicinski fakultet, Institut za zdravstvenu zaštitu dece i omladine, Novi Sad
Prenatal classification of the HLA system DR locus and its association with HLA DR3, DR4 and DR3,4 for insulin dependent diabetes mellitus is used in genetic consultations for the determination of genetic risk. Our patient was a pregnant woman who had a child with diabetes (HLA classification DR3\4). She wanted to know the possible risk for the next child. She was recommended a prenatal classification of the HLA system by the method of cordocentesis in the 20th week of pregnancy. The HLA classifications in fetal blood was DR 4,6. The risk for this locus was 29% but if the DR locus had been 3, 4 the risk would be doubled (68%). The patient was informed of the risk and she decided to have the child.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
GENETIC INVESTIGATIONS OF CARDIOVASCULAR DISEASES
Vesna KNEŽEVIĆ 1, Vukosava DIKLIĆ 2, Marija KOSANOVIĆ 2 i N. VOJVODIĆ 3
1 Medicinski centar, Mladenovac
2 Institut za biologiju i humanu genetiku, Medicinskog fakulteta, Beograd
3 Gradski zavod za gerontologiju, Beograd
Familial grouping of Cardiovascular Diseases (CVD) and CVD risk-factors is a well known phenomenon. Our family study was undertaken in 132 young women who had a hypertension and some of them had other CVD risk factors as well. The control group consisted of 74 young of 74 young women without hypertension. Familial grouping of CVD risk factors and CVD was investigated among 642 first degree relatives and 1039 second degree relatives of probands and 378 first degree relatives and 654 second degree relatives of control subject. The prevalence of hypertension in the first and second degree relatives of patients was significantly higher than in the relatives of control subject (p<0.001, rr 1.7; p<0.002, rr 1.6). The prevalence of CVD was significantly higher only in the mothers of patients (p<0.02). Comparisons of the lipid status in examined and control groups showed that in the hypertensive group mean values of triglycerides were significantly higher and levels of HDL-cholesterol significantly lower, while levels of both serum total cholesterol, and LDL cholesterol were not significantly higher. The part of these investigations which relates to the association of lipid levels and DNA polymorphisms for apo B was published in Genetika, Vol 25, 1, 1-19, 1993.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
THE PARENTAL ORIGIN OF THE X CHROMOSOME IN A PATIENT WITH A ROBERTSONIAN TRANSLOCATION AND TURNER'S SYNDROME
M. KRAJINOVIĆ 1,2, K. IVANOVIĆ 3, L. MESTRONI 2, V. DIKLIĆ l i J. NIKOLIŠ l
1 Institut za biologiju i humanu genetiku, Medicinski fakultet, Univerzitet u Beogradu, Beograd
2 Internacional Centre for Genetic
Engineering and Biotechnology, UNIDO, Trieste, Italy
3 Pedijatrijsko odeljenje Opšte bolnice, Pančevo,
We give a report on a proband with both a paternally inherited Robertsonian translocation and Turner s syndrome. A study of the parental origin of the X chromosome performed by microsatellite analysis indicates paternal origin of the X chromosome (Xpat). The occurrence of chromosome aberrations as a consequence of interchromosomal interactions is discussed.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
THE REGISTRATION OF CHROMOSOMAL ABERRATIONS USING THE METHODOLOGY OF D.S.BORGOANKAR
A. KRSTIĆ, M. KOVAČEVIĆ, G. RACA, F. POPIĆ-PALJIĆ, J. JOVANOVIĆ-PRIVRODSKI, R.
ALEKSIĆ, N. ČAMBER, R. MADŽAR, V. ČIHI, Lj. GAĆINA, N. MITROVČAN i Lj. MALEŠEVIĆ
Data about chromosomal aberrations from cytogenetic laboratories from all over the world has been collected and registered for more then 25 years in a computer center at the University of Delaware. The idea about the registration system comes from Prof. D.S. Borgoankar, who is the Head of the Cytogenetic laboratory at the Medical Center in Delaware. In order to benefit from the advantages of this efficient system for registration, we developed practical software for PC and PC compatible computers. This software is designed to register, sort and print data about chromosomal aberrations. It uses the methodology of D.S. Borgoankar and has functioned since 1993. Information about most chromosomal abnormalities diagnosed so far in the cytogenetic laboratory of the Children's Hospital in Novi Sad are already stored in its data base (total of 554 cases for the period from 1969 until March 1994). Work on the registration goes on continuously and data for the year 1993 were sent to the World's Registry in Delaware. Using REPOS (from the English word REPOSITORY) we obtained a good insight into the result of our work. We also have capability for a better follow-up in the future. We have improved our communication with the World's Central Register so that we can use all of its advantages. This software can be used for the registration of chromosomal aberrations diagnosed in all laboratories on the territory of Yugoslavia.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
MORAL AND ETHICAL ASPECTS OF PRENATAL DIAGNOSIS IN HEREDITARY DISEASES
M. KRSTIĆ, Stefanija MARKOVIĆ i Slavenka ADŽIĆ
Institut za ginekologiju i akušerstvo KCS, Beograd
Prenatal diagnosis of inherited diseases includes interventions which have ethical and logical concerns. When disorders of the foetus are found, a decision about its fate needs to be made. It is usually made on the basis of genetic analysis and the knowledge that the disorders will become apparent during later life. According medical to ethics, prenatal diagnosis and the decision about the termination of the pregnancy, fall within the domain of genetic counselling. The decision is usually made on the basis of doctor's and geneticist’s opinions, as well as the attitude of parents who are told about which disorders are expected in their child. Doctors and geneticists have many dilemmas about their standpoint and attitude in counselling the parents to terminate the pregnancy. These problems concerning the treatment of chromosome constitution XYY, XXX and other variants including different types of mozaicisms, appear at the Institute of Genecology and Obstetrics. The authors want to stress the difficulties in decision making in the mentioned cases including moral dilemmas and the question of who is, in general, to judge whether a life is worth living or not.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
CYTOGENETIC INVESTIGATIONS IN
MEDICAL GENETICS
Živana LACA
Centar za Medicinsku genetiku
Institut za mentalno zdravlje, Beograd
Cytogenetic methods are widely used in various studies in our Center for Medical Genetics. 1. Investigates of the etilogy of certain diseases and syndromes. The development and application of differential staining of chromosomes anabled the detection of a large number of numerical and structural chromosomal aberrations in certain diseases and syndromes. In research of this type we are faced with structural changes which require the involvement of experts in different fields (cytogeneticst, molecular biologist, endocrinologist, etc.). 2. The prevention of the birth of children with chromosomal aberrations - Prenatal analysis of fetal karyotype is made in all cases where indications exist. It is rather difficult to give advice regarding certain findings and their interpretation. 3. The prevention of malignancy - In the group of patients with gonadal dysgenesis and dysfunction, cytogenetic analysis reveals the presence of a male karytype in some female patients with gonadal dysgenesis or testicular feminisation. This enables timely surgical intervention and elimination of nondifferentiated gonads which show a tendency to malignancy. 4. An explanation of the development of certain disturbances at a chromosomal level - Analysis of chromosomal polymorphisms and particularly the application of the NOR staining method enables a checking of the origin of the chromosomal non-disjunction. 5. A follow-up of some processes at the cell level like mitotic division of aberrant chromosomes, cell kinetics in some syndromes, etc. 6. Testing hypotheses about the genesis of chromosomal aberrations. 7. Investigation of the mutagenic effects of some agents. Cytogcnctic analysis significantly contributes to problem solving in ontogenetics, evolution genetics, etc.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
THE USE OF FLUORESCENT IN SITU HYBRIDIZATION FOR MARKER CHROMOSOME IDENTIFICATION IN PATIENTS WITH TURNER'S SYNDROME
Milena LUDOSKI 1, Ljiljana LALIĆ 2, Vera POPOVIĆ 2, V. JURUKOVSKI 4 i Angelina NOVAK 1
1 Laboratorija za Humanu Genetiku Instituta za Hematologiju i Instituta za Endokrinologiju, Univerzitetsko - kliničkog centra, Beograd,
3 Laboratorija za Citogenetiku, Vojno - medicinske Akademije, Beograd
4 Inslitut za Molekularnu Genetiku i Genetičko inženjerstvo, Beograd
During the identification of marker chromosomes in patients with congenital disorders cytogenetists have been faced with a lot of difficulties, despite progress in various specialized staining techniques. However, the new method, fluorescent in situ hibridization (FISH) with chromosome-specific DNA probes offered a solution to this problem. The two patients, described below, have phenotypes of Turner's syndrome and mosaic karyotypes. Only slightly more than naif of the patients with Turner's syndrome are monosomic for the X chromosome. Most of the remaining patients have a second lineage of cells which contain a structurally normal or abnormal X chromosome. Cytogenetic analysis performed by HG-banding techniques, revealed mosaic karyolypes, 45,XO/46,X,r(?), in both patients. Also, in both cases the discovered marker chromosome resembled ring chromosomes. The only differences were in the percentage of abnormal cells and size of ring chromosomes. Fluorescent in situ hybridization was performed on the same slides after cytogenetic analysis. The biotinylated probe, pBam X5, was composed of alphoid, tandemly repeated sequences unique to the X-chrornosome centromere. The ring chromosomes were shown to be derived from the X chromosome in both female patients. Routine cytogenetic studies, using the Q-banding technique, excluded the presence of the brilliantly fluorescent end of the Y-chromosome long arm on these two ring chromosomes. In conclusion, a combination of cytogenetic methods and FISH with chromosome-specific DNA probes significantly increases marker chromosome identification and provides a means for more accurate diagnosis.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
RING (15)-A CASE REPORT
B. LUKIĆ, Nada BARJAKTAROVIĆ, Nada
TODOROVIĆ i Vitana KOSTIĆ
Kliničko-bolnički centar "Zvezdara", Beograd
The authors present the case of a female newborn with a low body weight of 2300g at birth, two weeks before term. Body length was 43cm, head circumference 30cm. There were two signs of malnutrition, visible stigmata occurred only on the extremities which were short, especially the upper arms, and both feet were with an outstanding metatarsus various. There was a small umbilical hernia. All analyses were within physiological limits. Neurological status O.K. Cytogenetic analysis showed the karyotype 46,XX,r(15)(pl2,q26). Literature data of this syndrome are very few, but the clinical features reported arc in agreement with our case. Except for an obvious deformity of both feet, other phenotypic changes were not present. For the ring formation to appear, a double deletion has to occur, both on "p" and "q" arms, which means that some deficit of genetic material certainly exists.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
CHROMOSOMAL ABERRATIONS IN THREE OVARIAN TUMORS
Ljiljana LUKOVIĆ 1, Jelena MILAŠIN 2, N. ARSENOVIĆ 3 i Vukosava DIKLIĆ 1
1 Institut za Biologiju i Humanu genetiku, Medicinskog i
2 Stomatološkog fakulteta, Beograd,
Ginekološko-akušerska klinika, KBC, Beograd
Three samples of ovarian carcinomas were subjected to cytogenetic analysis. Two were histological serous adenocarcinomas and one a cystadenocarcinoma. The patients were not under chemo- or radiotcraphy. Chromosomes were obtained by direct preparation and stained using the GTG technique. Extensive and complex numerical and structural alterations were seen in all specimens. Chromosomal numbers were in the range of 27-29 (in one mitosis 400 chromosomes). Gross marker chromosomes characterized all tumours. We were able to indentify always, partially or completely, the origin of marker chromosomes, due to complex rearrangements involving small portions of chromosomes. Chromosomes #1, #3, #6, #7, and #11 were most frequently involved in structural aberrations. Double minute chromosomes (DMs) were seen in one patient and a homogeneously staining region (HSR) was found in another.
The chromosomal aberration 6q- described as a specific change in ovarian tumours, was found in 2 cases. Furthermore, the recurrence of specific regional chromosome losses and breakpoints suggests a localization of genes that play a role in the formation and progression of some ovarian tumours.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
GENETIC HOMOZYGOSITY AND NORMAL vs. PATHOLOGICAL HUMAN VARIATION
D. MARINKOVIĆ, D. JOVANOVIĆ, S. CVJETIČANIN i S. BRANKOVIĆ
Biološki fakultet Univerziteta u Beogradu, Beograd.
The degree of individual genetic homozygosity has been estimated according to the presence of cca. 30 qualitative homo-recessive characters ("HRC-TEST") in sample of healthy and diseased individuals. In the majority of such studies a significant increase in HRC's has been determined among patients with a number of diseases (e.g., Endemic nephropathy, Muscular dystrophy, Diabetes mellitus, Chronic lymphcytic leukaemia, Congenital hip dislocation, Ca uteri). A similar or lower mean homozygosity has been established in samples of patients with Acute appendicitis, Bronchial asthma, Bronchogenic carcinoma, Breast cancer, showing, however, a significant difference in the frequency distribution of the studied traits. Further applications of HRC-testing may have a practical use in genetic counselling, since they seem to be in correlation with the individual's resistance to different diseases.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
THE INFLUENCE OF SEX CHROMOSOMAL ANOMALIES ON SPERMATOGENESIS
Miroslava MIĆIĆ
Institut za biologiju i hum. genetiku, Stomatološki fakultet, Beograd
Sex chromosomal abnormalities were found among males treated for infertility. Different deletions of Yq were the most frequent structural anomaly usually affecting the patient's fertility. Other structural aberrations are rare and we have observed the following: 47,XisoXqY; 45,X 46,XisoYq; 45,X 46,XringY; 46,Xt(Y;13). All patients had azoospermia. Testicular biopsy showed the presence of a spermatogenic process in all cases except in the patient with the 47,XisoXqY karyotype. Patients with anomalies of Y chromosome and X monosomy cell line had all spermatogenic stages with a lower number of cells in the later stages of the process. The 45,X cell line was not observed in dividing testicular cells. Analysis of diakinesis-Metaphase I showed bivalent anomalies which affected the later stages of spermatogenesis. The influence of Y chromosome aberrations on the spermatogenic process and fertility of patients is discussed.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
THE NUCLEAR VOLUME IN PATAU'S SYNDROME
D. MIHAILOVIĆ, Z. STANKOVIĆ, B.
ĐORĐEVIĆ, V. MIHAILOVIĆ i M. ČEKEREVAC
Institut za patologiju Medicinskog fakulteta, Niš
Kariometric parameters have sometimes been used in histopathology, because of the well-documented correlation between the nuclear volume and the quantity of DNA. In this study, the mean nuclear volume of various cells was estimated in trisomy 13 patients (patau's syndrome). From 3 newborn infants, an unbiased estimate of the mean nuclear volume (Vv) was obtained after standard formalin fixation, paraffin-embedding, sectioning and hematoxylin-eosin staining, using the equation VV=I03∙π/3 where I0 is the length of an intercept measured in a random direction trough a test point, which hits a nucleus. Compared with control newborn infants, there was a significantly lower mean nuclear volume of hepatocytes and endocrine cells of the pancreas in patients with Patau's syndrome. These results indicated that DNA content and mean nuclear volume may provide partly independent biological information.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
THE FREQUENCY OF ACROCENTRIC ASSOCIATIONS IN WOMEN WITH SPONTANEOUS ABORTUS
V. MIHAILOVIĆ, Z. STANKOVIĆ, D. MIHAILOVIĆ i D. STOJANOVIĆ
Institut za patologiju Medicinskog fakulteta, Niš
In the human karyotype 5 pairs of acrocentric chromosomes have been show to carry nucleolar organizer regions and have a tendency for intragroup associations or clustering. It has been reported that the frequency of associations of acrocentric chromosomes decreases in older individuals, as compared to middle aged persons. The aim of this paper was to investigate the incidence of acrocentric associations in women with spontaneous abortions. Metaphases from lymphocyte cultures were studied. The frequency of satellite associations were higher in women with spontaneous abortions, compared to controls. We conclude that the frequency of satellite associations may indicate the possibility of nondisjunction of chromosomes during mitosis.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
H-ras GENE MUTATIONS IN HUMAN URINARY TRACT TUMORS
Jelena MILAŠIN 1, Nasta DEDOVIĆ 2, S. MIĆIĆ 3, B. DIMITRIJEVIĆ 2, Vukica DIKLIĆ l
1 Institut za biologiju i humanu genetiku,
Stomatološki fakultet, Beograd
2 Institut za nuklearne nauke, Vinča, Beograd
3 Urološka klinika Medicinski fakultet, Beograd
Alternation of ras oncogenes and subsequently of the encoded p21 proteins, has been implicated in the pathogcnesis of various human malignancies. Members of the ras gene family (H, K and N ras) become activated, i.e. acquire the transforming potential, by point mutations in codon 12, 13, and 61. The DNA from 32 urothelial cancers (transitional cells carcinomas of the bladder, urether and pelvis) and 22 kidney cancers (renal cell carcinomas) was screened for the presence of Ha-ras gene mutations. Using the technique of oligonucleotide hybridization on in vitro amplified tumor DNA, H-ras mutations at 12 and 13 were detected in 7 out of 32 transitional cell carcinoma specimens (22%) and in 3 out of 22 renal cell carcinoma specimens (13.6%) The predominant mutations were G->A transitions at codon 12, precisely GGC->AGC (glycine->serine) and GGC->GAC (glycine->aspartic acid) in urothelial tumors, and at codon 13 in kidney tumors (GGT->GAT, i.e. glycine-> asparagine). The 18 % overall incidence of mutated ras genes suggests that ras gene activation docs not represent the crucial event in urinary tract tumor development, at least not in the major part of the tumor population.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
THE REACTION OF HUMAN T LIMPHOCYTES TO MAFOSFAMIDE AND 5-FLUOROURACIL IN VITRO
Olivera MILOŠEVIĆ-ĐORĐEVIĆ l i Nada BARJAKTAROVIĆ 2
1 Prirodno matematički fakultet, Univerzitet u Kragujevcu, Kragujevac ,
2 Klinička bolnica grada Beograda, Beograd
The effects of the alkylating agent: mafosfamide and the nucleic acid analog 5-fIourouracil were tested, as they are the most frequently used drugs for chemotherapy of cancer. It is known than cyclophosphamidc does not act per se as an alkylant, but in vivo undergoes metabolic activation. Mafosfamide is a derivative of 4-hydrooxycyclophosphamidc and it is active in vitro and in vivo. We tested the ability of these agents to induct chromosomal aberrations in human lymphocyte cultures which were PHA activated or not. The drugs were tested in the cone, of 9 ug/ml for mafosfamide and 11,4x10 M for 5-fluorouracil. Analysis of the frequency of chromosomal aberrations in control nontreated lymphocyte cultures from the same persons was carried out concomitantly. Maosfamide and 5-flourouracil induced significant increases of chromosomal aberrations frequency in cultures which were not PHA activated in the period of treatment (5,7*** and 6,9***). In contrast to 5-flourouracil, mafosfamid induced a significant increases of chromosomal aberration frequency also in cells which were PHA stimulated in the period of treatment by the agents (9,9*** and 1.2). Mafosfamide significantly induced the following types of aberrations: chromosomal (49%) and chromatid breaks (36%), achromatid lesions (30,5%) and dicentric (2%). 5-fluorouracil induced aberrations only of the break type.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
A CITOGENETICINVESTIGATION OF CHROMOSOMES IN PATIENS WITH INCONTINENTIO PIGMENTI
S. MINIĆ 1. M. KOSANOVIĆ 2 i B. PETROVIĆ 2
1 Klinika za kožne bolesti, UKC, Beograd ,
2 Institut za biologiju i humanu genetiku, Medicinski fakultet, Beograd
Incontinentio Pigmenti (IP), also known as Block-Sulzberger syndrome or Bloch-siemens syndrome, Bloch Siemens syndrome, is a rare, complex genodermatosis, in which skin changes are often combined with other organ and structure anomalies of ectodermal origin. It occurs nearly exclusively in females. The high percentage of heredity (55,4%) and female to male ratio of 17:1 suggest that some genome anomalies could be the cause of the sickness. Although a many questions concerning IP have not been solved, our investigation of the family pedigrees of our probands attempts to confirm its heredity and discover the way it is inherited. We also examined their karyotypes (probands and their family members) in order to determine if they were positive for IP which), and whether it was due to chromosome aberrations or gene mutations. Our results indicated that 10 out of eleven affected examines came from families in which more than one family member was affected which confirmed the heredity of the disease. A pedigree analysis was in favour of the X linked do minant type of heredity. IP is transmitted from mother to daughter. There are no affected males in the proband's pedigrees. Although heredity has been proved, cytogenetical analysis of all affected examines showed a normal karyotype.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
Ag- NORs IN NORMAL HUMAN POPULATION
J.NIKOLIŠ 1, V. KEKIĆ 2, S. MIĆIĆ 3, V. DIKLIĆ 1, M. RISTANOVIĆ l
1 Institut za biologiju i humanu genetiku Medicinskog fakulteta Univerziteta u Beogradu, Beograd
2 Biološki fakultet, Univerzitet u Beogradu,
Beograd
3 Urološka Klinika UKC u Beogradu, Beograd
The activity of nucleolus organizers regions (NORs) in lymphocytes of 49 males aged 11-74 years and 48 females aged 18-74 years was analysed. The aim of this study was to examine if there are any variations in the frequency of Ag-stained NORs in different age and sex groups. The results obtained indicate a significant difference in the number of active NORs between males and females. There is no correlation, however, between the number of active NORs and the age of the individual.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
INTERPHASE CYTOGENETICS: THE DETECTION OF THE GENOMIC CONSTITUTION OF THE OVARY (PARAFFIN-EMBEDDED SECTIONS) IN A PATIENT WITH TURNER'S SYNDROME AND CHROMOSOMAL MOZAICISM
Angelina NOVAK 1, Đ. KOKAI 2, Vera POPOVIĆ 3, Milena LUDOŠKI l, V.
JARUKOVSKI 4
1 Laboratorija za Humanu Genetiku, Institut za Hematologiju, Beograd
2 Odeljenje Patologije Ginekološko-Akušerske klinike, Beograd
3 Institut za Endokrinologiju, Univerzitetsko-Kliničkog Centra, Beograd i
4 Institut za Molekularnu genetiku i Genetičko inženjerstvo, Beograd
The difficulty of detecting sex chromosome mosaicism cytogenetically hinders the establishment of an acceptable explanation for the phenotype-genotype discrepancy amongst these patients. Fluorescence In Situ Hibridization (FISH) permits the genomic identification of patients with mosaic karyotypes in interphase nuclei by utilising the X-chromosomc-specific DNA probe ("interphase cytogenetics"). We evaluated the efficiency of "interphase cytogenetics" in the detection of the genomic constitution of the ovary from a patient with Turner's syndrome with mosaicism (46,XX/46,XO) that was previously established by blood limphocyte karyotyping. We used alphoid rcpctitative sequences specific for the X chromosome and a biotin-labclled probe, PBamXS. An examination of ovarian sections and blood lymphocytes by FISH showed persistence of both cell lines 46,XX/45,XO in each. The genomic constitution of the germ cell/oocyte in an ovarian primordial follicle was shown to be normal (46,XX). Our results: l. showed a high applicability of interphase cytogenetics on paraffin sections, 2.indicatcd the possibility of genomic screening of diverse tissues for routine cytogenetic investigation, 3.offered a reliable methodological approach for defining an accurate percentage of abnormal karyotypes in mosaicism of different organs and non-dividing tissues.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
A CASE OF MONOSOMY 22
Ivana NOVAKOVIĆ 1, Olga ANTONOVIĆ 2, Slavenka ADŽIĆ 2 i Svjetlana MAGLAJIĆ 3
1 Institut za biologiju i humanu genetiku, Medicinski fakultet
2 Institut za ginekologiju i akušerstvo, KCS, Beograd
Univerzitetska dečja klinika, Beograd
A new case of monosomy 22 is described in a female newborn. The child had facial dysmorphy, bilateral corneal opacity, elongated, disproportional toes and muscular hypotony; a systolic heart murmur was detected. Chromosome analysis showed the karyotype 45,XX,-22,inv(9)mat. The newborn died shortly after birth. A post-mortem examination showed a persistent truncus arteriosus type HI, an atrial septal defect and a persistent left superior vena cava. Our case is compared with other cases of monosomy 22 with or without mosaicism.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
A GENETIC SURVEY OF A FAMILY WITH REPEATED POLYDACTYLISM
V. NOVOSEL
Medicinski institut Univerziteta Crne Gore, Podgorica
A case of a family with a marked frequency of occurrence of polydactylism on both hands and feet has been described. Anamnesic data indicate that the occurrence of this phenomenon originates from the second (II) generation, and it is completely developed in the fourth (IV) generation in three sisters, who were subject of our examination. This anomaly becomes apparent in the form of a sixth finger; from the x-ray one can clearly sec the developed metacarpal bone from the distal side of their arms and legs. The analysis of metaphase chromosomes and sex chromatin indicates this to be a normal karyotype with 46,XX. From the data given one can conclude that this anomaly has occurred as a consequence of a gene mutation which became apparent in the fourth generation.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
FAMILIAL 46,XY GONADAL DYSGENESIS
Vesna PENDIĆ 1, Vera
POPOVIĆ 2 i Angelina NOVAK 1
1 Laboratorija za Humanu Genetiku, Institut
za Hematologiju, Beograd
2 Institut za Endokrinologiju, Univerzitetsko-Kliničkog Centra,
Beograd
The Swyer syndrome, in its complete form is characterized by female phenotype (bilateral tubes, a uterus and a vagina), primary amenorrhea and 46,XY karyotype. This prevalence of gonadal neoplasms: gonadoblastoma and germinoma, is high. We present a patient with a normal female phcnotype, primary amcnorrhea and 46,XY karyotype. This patient, also, had a unilateral ovarectomy with the diagnosis of disgcrminoma. Cytological investigation, using the Q-tcchnique, confirmed that epithelial cells from the patient's buccal smear contained a brilliantly fluorescent "Y-body". Cytogenetic investigation, with HG-banding, of blood lymphocyte metaphases revealed the 46,XY karyotype without any structural rearrangements on the Y chromosome. The discrepancy between genotype and phenotype may be explained by investigations of the patient's sister with the same clinical (normal female with primary amenorrhea) and cytological (46,XY) status. Finally, all these facts indicate that 46,XY, gonadal dysgenesis is a familial disease inherited as an X-linked recessive trait.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
ABO BLOOD TYPE FREQUENCY IN SOME LUNG DISEASES
D. PEŠUT 1, D.
MARINKOVIĆ 2
1 Institut za plućne bolesti i tuberkulozu, Klinički centar
Srbije, Beograd
2 Biološki fakultet P.M.F. Univerzitet u Beogradu, Beograd
The frequency of ABO blood types was analysed among patients (Pts) with lung tuberculosis (LTB), hydatid lung disease (HLD) and bronchogenic carcinoma (BC). The diagnoses had been established at the Institute of Lung Diseases and Tuberculosis of Serbia and all of the cases. The frequency variations of ABO blood types in Pts's groups were compared to those found in Yugoslav population and the differences tested using the χ2 test. In the group of Pts operated on for HLD (N=84) blood type A was significantly decreased comparing to the frequency found in the Yugoslav population. Blood type B and AB frequencies were increased but without significance. In the group of LTB Pts including 79 individuals with both the fibrocaseous cavernous (FC) form and specific pleural disease (PD), blood type O was significantly decreased compared to the frequency in the general type population. A significantly increased frequency of blood type B was found among LTB Pts with the FC form of the disease, while blood type O was increased in LTB Pts with PD. In the group of BC Pts (N=105) there was no significant difference in ABO blood type frequencies compared to that in the Yugoslav population, i.e, the differences decrease with increase Pis' number. The result favour genetic predisposition / resistance to LTB as well as to the form of the disease and are an addition to controversial findings in the last two decades which have not taken into account the form of the disease. This study speaks in favour of a genetic influence even in parasitic lung disease. Individuals with blood type A seem to be more resistant to HLD than those with B and AB types.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
GENETIC COUNSELLING OF MARRIED COUPLES IN CASES OF EFFECTS OF HARMFUL ENVIRONMENTAL NOXES
F. POPIĆ-PALJIĆ, A. KRSTIĆ, J. JOVANOVIĆ-PRIVRODSKI, R. I ALEKSIĆ i G. RACA
Institut za zdravstvenu zastitu dece i omladine, Novi Sad
The main purpose of genetic counselling work is the prevention of the birth of children with hereditary and congenital anomalies. In order to estimate the quantity of teratogenous environmental effects, we have made conducted an analysis of couples who visited our genetic counselling service in a four year period because of the effects of harmful environmental noxes during pregnancy. Out of the total of 995 couple's, 362 visited us because of possible harmful environmental effects. Due to the use of medications and work with harmful chemicals 159 couples come to see us, which accounted for 16,75% of the total number of women examined in our service. Out of that number 30,82% took antibiotics, 17,6% sedatives and anxiolytics, 13,8% analgetics. Antiallergies were used less (6.28%), antieptileptics 4,4%, hypnotics 1,25%. Medications which could not be included in these groups made up 21,38%. Because of harmful chemicals, paints and lacquer, 4,4% women came to see us. Pregnancy infections or contacts with infectious agents brought 127 women (13,29% from the examined group). The most common causes of their coming to our service were rubella infections (15,74%), varicella (14,96%) and herpes simplex type I 13,4%. Influenza was frequent (18,11%), as well as other infections (bacterial sore throat, sinusitis, urinary infection and respiratory tract infection 27,55%. there were no cases of AIDS and Hepatitis B. Due to the fear of X-Rays or other radiation exposure 76 couples came (7.96%). The fetus was directly radiated only in the case of pelvic X-ray diagnostic procedures in 22 (28,95). Considering the number of pregnant women who are exposed to teratogenic stimuli (according to data in the literature), we think that the number of women coming for this reasons should be greater.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
THE USE OF MODERN SOFTWARE IN DIAGNOSING RARE SYNDROMES
F. POPIĆ-PALJIĆ, A. KRSTIĆ, J. JOVANOVIĆ-PRIVRODSKI, G. RACA i R. ALEKSIĆ
Institut za zdravstvenu zaštitu dece i omladine, Novi Sad
In our everyday work in the genetics department of our Institute we use two SOFTWARE PROGRAMMES which are of grca help to us in diagnosing rare syndromes. They are LDDB (London Dysmorphology Data Base) of British authors and POSSUM (Pictures of Standard Syndromes and Undiagnosed Malformations) by Australian authors. The SOFTWARE functions as follows: all the patients symptoms are fed in and the machine offers a certain number of syndromes on the basis of the given symptoms. As the given syndrome is frequently unknown, on can use the syndrome vocabulary that contains a detailed syndrome description, data with references from the world literature, examples of patients with a corresponding syndrome, at different ages including patients photographs and their X-rays. Diagnosis is made by detailed analysis and comparison of the patient understudy to those obtained from the software. In addition to the syndrome vocabulary, the software contains a symptom dictionary as well. Both of them play an important part in education. In one of our patients born on September 11, 1986 in Bugojno, who was hospitalized because of a chest deformity with frequent respiratory infections, the diagnosis of Spondylothoracic dysplasia was made thanks to POSSUM. On the basis of the given symptoms: postnational growth arrest, abnormal rib number (ribs 3-8 are absent), scoliosis and udescended tests, the software offered these diagnoses: l. Jarcho-Levin syndrome(Spondilothoracic dysplasia) and 2.Simpson-Colahi Behmel syndrome. After careful comparison we chose the diagnosis of Spondilothoracic dysplasia which is autosomal-recessively inherited.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
FRAGILE SITES AND ONCOGENES AS A POSSIBLE RISK FOR NEOPLASIA IN CARRIERS OF BALANCE TRANSLOCATIONS
Branka POPOVIĆ, B. GARZIČIĆ i Biljana SPREMO
Institut za onkologiju i radiologiju Srbije, Laboratorija za kliničku i eksperimentalnu onkologiju, Beograd
The hereditary theory of oncogenesis observes the development of neoplasia as a microevolution cither at the oncogene level or at the chromosome level. One type of primary translocation of cells is chromosome translocation (HGL, BURKITT LYMPHOMA etc.). Since primary translocations in cancer include reciprocal translocation on fragile sites (fra), the question is: are FRE a risk for the development of cancer in the human population? We detected translocation sites on chromosomes 1,2,6 and 7 in 1000 constituve balanced cariotypes, and in 100 leukemic cariotypes, and we wished to know if FRA and ONC are present? In 1000 constitutive balanced cariotypes we found an average of 70% translocations on FRA, and 30% on FRA and ONC. Most of the primary translocations in 100 leukemic cariotypes were on sites with FRA and ONC, but break points in oncogenesis may or may not include sites with FRA. The same break points on chromosomes can produce different phenotypes, but the main condition for malignant expression is the break region on a specific site within the ONC itself.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
AN INDUCTION OF FRAGILE SITES ON HUMAN CHROMOSOMES BY APHIDICOLINE AND CYTOSINE-ARABINOSIDE
G. RACA, A. KRSTIĆ, B. GARZIČIĆ, F. POPIĆ-PALJIĆ, J. JOVANOVIĆ-PRIVRODSKI, R. ALEKSIĆ, N. ČAMBER, R. MADŽAR, V. ČIHI, Lj. GAĆINA, N. MITROVČAN i Lj. MALEŠEVIĆ
The capacity of nucleoside - analog 1-bcta-arabinofuranosyl cytosine to induce fragile sites of the aphidicoline type (type 4) on human chromosomes is well documented. Our goal was to check if some fragile sites from this group show special sensitivity to cytosine-arabinoside. We wanted to ascertain if some fragile sites show different expression depending on the agent used for induction (aphidicolin or cytosine-arabinoside). The fragile site most sensitive to induction by aphidicoline was fra 3(pl4.2). The fragile site at Xp22 turned out to be the most sensitive to cytosine-arabinoside. There is a possibility that these differences are based on different molecular composition of DNA segments at different fragile sites (fragile sites especially sensitive to cytosine-arabinoside are expected to be rich in GC base pairs). These results are especially important for the development of more efficient techniques for cytogenetic diagnosis of fragile X. Since we know today that the mutation in fragile X patients consists of amplification of CGG isles, we consider that cytosine-arabinoside is one of the substances that should be tested for its capacity to induce fragile X.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
AN APPLICATION OF THE RECOMBINANT DNA METHOD IN THE PRENATAL DIAGNOSTIC OF CYSTIC FIBROSIS
Dragica RADOJKOVIĆ 1, Ana SAVIĆ 2, J. SAVIĆ 1, P. MINIĆ l i B. BRUKNER-DABOVIĆ
1 Institut za zdravstvenu zaštitu majke i deteta Srbije, Beograd
2 Institut za molekularnu genetiku i genetičko inženjerstvo, Beograd
Cystic fibrosis (CF) is one of the most common autosomal recessive diseases (1 in 2500 live births gives rise to an affected child). Affected persons are homozygous for the mutated CF gene. Regarding the severity of this disease and the high risk (1:4) of couples who are hcterozygons for the mutated gene to have an affected child, it is essential to perform prenatal diagnosis of CF. Genetic prenatal diagnosis of CF can be performed in the first and second trimester of pregnancy. It is possible to perform diect and indirect genetic analysis of the presence of the mutated gene. Direct detection is performed by searching for known mutations within the CFTR gene (F508 is the most frequent one). Indirect detection is performed using RFLPs (Restriction Fragment Length Polymorphism) closely associated with the CFTR gene. The development of recombinant DNA techniques, particularly PCR (Polymerase chain reaction) provide precise and fast prenatal diagnosis of CF (within 48 hours). We present our results of the prenatal diagnosis of CF using recombinant DNA methods in the first and second trimester of pregnancy. We discuss the advantages, problems and a proper approach to prenatal diagnosis if this disease.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
A RARE VARIANT OF KARYOTYPE 47,XY,13 IN AML (M2 TYPE)
S. RADULOVIĆ, Nada BARJAKTAROVIĆ i Vitana KOSTIĆ
Kliničko - bolnički centar "Zvezdara", Beograd
A 74 year old patient was diagnosed as ANLL (M2) based on: peripheral blood and bone marrow findings, cystochemical staining and immunophenotyping. Cytogenetic analysis was performed before introducing cytogenetic therapy, both conventional and G-banding techniques on 50 bone marrow metaphases. Trisomy 13 was found as the only disbalance of the karyotype. Neither numerical nor structural changes of remaining chromosomes were noticed. According to the literature data, chromosome 13 is seldom present in chromosome rearrangements in AML, so just the conclusion that this variant of karyotype is connected with poor prognosis is of great importance.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
THE SEGREGATION TYPE, CHROMOSOME AND BREAKPOINT DISTRIBUTION IN PROGENY OF CARRIERS OF RECIPROCAL TRANSLOCATIONS
O. RISTIĆ, Ž. LAĆA, S. NIKŠIĆ, N. ARANĐELOVIĆ i I. TIMOTIJEVIĆ
Institut za mentalno zdravlje, Beograd
A follow-up study for 15 years, resulted in the detection of 35 reciprocal translocations (RT), among 1567 couples with a fertility problem (2,2%). The segregational analysis was done using the Jalbert and Sclc model. Out of the 35 RT the adjaccnt-1 (A-l) type was predicted in 88,5% adjaccnt-2 (A-2) in 3% and 3:1 segregation type in 8,5%. The 3:1 segregational type was significantly less frequent in comparison with similar studies. Regarding the progeny with unbalanced karyotypes, confirmation between the predicted and obtained model was detected in all 8 possible cases. Concerning the chromosome distribution, the most frequent were 2,4,21 which is consistent with Davis RT sample. Chromosome 22 was even less frequent than chromosome 21 leading to discrepancy concerning Davis sample. Break point (BP) positioning was determined using Neri's model in which chromosomes were divided into terminal (T), medial (M) and centromeric (C). A T/S combination was present in 60%, S/S in 17,2%, T/T in 11,4% and C/S and C/T in 5,7% each. The probability of obtaining a normal phenotype and malformed progeny was estimated regarding the total number of pregnancies, liveborn infants and the sex of the carrier parent. Concerning liveborn infants, the risk for an unbalanced karytype was 14,3% while the percentage of infants with normal and balanced karyotypes was equal at 42,8% each. The spontaneous risk rate was 54,5%.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
APHIDICOLIN FRAGILE SITES AS TARGET POINTS FOR IONIZING RADIATION
Biljana SPREMO i B. GARZIČIĆ
Institut za onkologiju i radiologiju, Laboratorija za kliničku i eksperimentalnu onkologiju, Beograd
The application of therapeutical doses of ionizing radiation in 13 patients with Carcinoma Portio Vaginalis Uteri (Ca PVU), indused chromosome mutations in peripheral T lymphocytes. These changes included the occurrence of a high percentage of: dicentric, polycentric and ring chromosomes, acentric fragments and heteroploidy with aberrant chromosomes. Quantification of chromosome aberrations during radioteraphy, showed an increase of their percentage. The quality of chromosome rearrangements was detected by the G-band technique and we used it to find out the break regions on chromosomes which produced dicentrics as the most frequent type of aberrations. We analyzed more than 1300 mitoses in 13 patients during radiotheraphy and found that the break points were not random. Most of the detected break points were on fragile sites of the aphidicolin type.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
LUPUS ANTICOAGULANT ANTIBODIES TO CARDIOLIPIN AND THEIR CLINICAL MANIFESTATION IN SLE PATIENTS AND MEMBERS OF THEIR FAMILIES
L.N. STOJANOVIĆ 1, E. L. NASSONOV 2 i M. STANIĆ 1
1 Institut za reumatologiju Akademije medicinskih nauka Rusije, Moskva
2 Institut za biologiju Medicinskog fakulteta, Beograd
The problem of the discovery of different immune disorders in normal family members of SLE patients and the increased concentration of rheumatic diseases in these families is one of the most difficult aspects in the understanding of SLE pathogenesis. Taking into account the increasing interest in lupus anticoagulant (LA), considered to be an acquired autoantibody-inhibitor of the prothrombinase complex , we included in our study 5 probands with cer SLE (2 male, 3 female) all LA-positive. Probands were 23-34 (mean value 27) yrs old with a disease duration of 2-20 (mean value 8,4) yrs. Three of them (1 male, 2 female) had kidney involvement (during 0,5-11 yrs). Inn all patients elevated levels of anti-DNA, anticardiolipin antibodies were revealed, and 3 had positive ANA. We studied 11 relatives of the 1st degree, and the following clinical symptoms were revealed: Raynaud's phenomenon (5), SLE (1+1 not included in the study who died from SLE in 1969), cryoglobulinemic hemalytic syndrome (1), varication (1), ankylosing spondyloarthritis (1), osteoarthrosis (1) , hypertension (1). Two relatives had noclinical symptoms (but 1 had laboratory changes). We also discovered the following laboratory disorders: 6 certain and 1 doubtful positive LA out of 10, 3 certain and 1 doubtful for positive anticardiolipin antibodies out of 9. We also found such immune disorders as: elevated anti-DNA antibody(S), ANA (2), cryoproteins (l), rheumatoid factor(l). The obtained data permit us to suggest that there can be a correlation between different clinical immunogenetical subtypes of SLE and the possibility of finding clinical and/or laboratory changes in the relatives of SLE patients. Therefore a concrete division of SLE in such subtypes may be helpful for prophylaxis and prognosis in SLE relativies.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
CYTOGENETIC PARAMETERS DURING DIFFERENT TREATMENTS IN PERINATOLOGY
Nada TODOROVIĆ, B. LUKIĆ i Nada BARJAKTAROVIĆ
Kliničko - bolnički centar "Zvezdara", Beograd
The authors present the results of an investigation of the level of sister-chromatid exchanges (SCE) after Progesterone treatment in pregnancy, after Syntocinon treatment in labor, and Luminal + phototherapy treatment in hyperbilirybinemia in newborns. A control group of healthy newborns had a SCE level of 5,55 ±0.62. the results of our investigations were: after Progesterone: 7,99±0.88 and in experiments in vitro up to 13.20±5.07. After Syntocinon treatment both in vivo and in vitro systems showed no significant increase of SCE-s. Luminal therapy also did not show any damage at the genetic level: 5.39±1.84, but phototherapy elevated the level of SCE-s to 7,66±2.18. Taking these results into account, the authors suggest a high censure in the application of Progesterone, and serious evaluation of indications for the use of phototherapy and also its duration.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
MISMATCH RECOGNITION EFFICIENCY. III. MISMATCH RECOGNITION EFFICINECY IN PLANT SUBCELLULAR ORGANELLES
Desanka BOŽIN i Gordana CEROVIĆ
Institut za nuklearne nauke Vinča, Beograd
Chloroplasts and mitochondria of most land plants, have their own DNA which is different from nuclear DNA. They have their own systems for DNA replication, transcription and translation. In fact most of the proteins found in chloroplasts are coded by nuclear genes, synthesized in the cytoplasm and imported into the plastids. A minority of chloroplast proteins are in fact of chloroplast origin, leading to questions about the organization and information content of chloroplast DNA. The studies of DNA repair in plants were limited to the repair of pyrimidine dimmers, strand breaks and alkylating agents damage. Very recently, we demonstrated in vitro mismatch-stimulated and mismatch-specific DNA breakdown in a nuclear extract from normal and transformed Nicotiana tobaco callus. These observations raise some intriguing questions: docs the mismatch repair activity exist in chloroplasts and how docs it function? Like bacterial systems? The same as nuclear? Therefore we sought for a mismatch-specific DNA breakdown in an extract from Triticum sp. ethyoplast. For this purpose we used the same model system as described in the two previous abstracts. Synthetic oligonucleotides were prepared in such a way that C • A and C • C mispairings were formed. We detected mismatch-stimulated and mismatch-specific activity in the extract from Triticum sp. ethyoplast with a reactivity order of preference: G:C <<< C • A << C • C. These findings are different from those which were demonstrated in our previous studies of plant nuclear extract recognition efficiency.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
MISMATCH RECOGNITION EFFICIENCY. I. MISMATCH RECOGNITION EFFICIENCY IN THE MAMMALIAN NUCLEUS
Desanka BOŽIN, Gordana CEROVIĆ i RADMAN
Institut za nuklearne nauke Vinča, Beograd
Institut Jacques Monod, 2 place Jussieu, 75251 Paris Cedex 05, France
A DNA base pair mismatch can be defined as any noncomplementary base pair in the DNA duplex. Mismatched base pairs arise in DNA in the course of DNA replication, recombination and deamination of 5-methyl-cytosine. The mechanisms for mismatch correction of replication errors have been postulated in well studied systems of E. coli, S. typhimurium and S. pneumoniae. Mismatch repair in eukaryotic cells is less well understood. Mismatch rectification has been demonstrated in Saccharomyces cerevisiae, vertebrate organisms and in higher plants. Therefore we sought for a mismatch-stimulated and mismatch-specific DNA breakdown of the DNA in the nuclear extract of rat liver. For this purpose we selected well studied K-ras gene and the vicinity of the frequently mutated gly-12 codon as the model system. Synthetic oligonucleotides were prepared in such a way that the gly-12 triplet was in the middle. A set of 19-mcr oligonucleotides was designed to allow for the concentration, upon annealing, of C • A G:A and C • C mispairings in the middle of the molecule. These elementary duplexes were legated into a size ladder which was the substrate in the in vitro mismatch recognition and mismatch-specific DNA breakdown assays in the nuclear extract of rat liver. We detected mismatch-stimulated and mismatch-specific activity in the nuclei of rat liver cells. An enzymatic analysis yielded a reactivity order of preference at physiological pH: G:C - G:A < C • C <<< C.A. Biochemical analysis suggested that it might be a functional homologue of the bacterial correction system(s).
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
MISMATCH RECOGNITION EFFICIENCY. II. CONFORMATION-DEPENDENT MISMATCH RECOGNITION EFFICIENCY IN THE MAMMALLIAN NUCLEUS
Desanka BOŽIN, Gordana CEROVIĆ i
RADMAN
Institut za nuklearne nauke Vinča, Beograd,
Institute Jacques Monod, 2 place Jussieu, 75251 Paris Cedex 05, France
Very recently, NMR spectroscopy and UV light melting studies have been used to probe the variability in the conformation and stability of the CA, GA and CC mismatches as a function of pH. These analyses have shown the presence of apH-dependent conformation and stability of noncomplementary base pairs. On the other hand, the presence of enzymatic systems specialized in the repair of noncomplementary DNA base pairs is well established. In order to understand the mechanism of mismatch recognition by the repair system, we have studied different pH-dependent conformations of CA, GA and CC containing heteroduplexes, corresponding to the sequence 25-43 of the K-ras gene by enzymatic assays. We detected conformation-dependent differences in the mismatch-specific recognition; efficiency. At pH 4.5, C:A, G:A, and C:C noncomplementary base pairs are protonated, G:A has GSyn: Aanti conformation and all base pairs have two hydrogen bondes. We have detected recognition efficiency and DNA breakdown with the order of preference: G:C = C:C - C:A < < G:A. At pH 7.5 C • A, G:A and C • C noncomplementary base pairs are not protonated. G:A has a Gami '• Aanti conformation and two hydrogen bonds. C • A and C • C have just one hydrogen bond respectively. We have detected the recognition efficiency and DNA breakdown with the order of preference: G:C = G:A < C • C <<< C • A. It is clear that mismatches provoke and promote their own repair.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
THE USE OF MULTIALLELIC HUMAN apoB GENE DNA POLYMORPHISM (3'HVR) FOR DNA TYPING
Sanja GLIŠIĆ, Ivana SAVIĆ i ALAVANIĆ
Institut za nuklearne nauke "Vinča", Laboratorija za radiobiologiju i molekularnu genetiku, Beograd
DNA polymorphism of the hypervariable region on the 3' end of the human apoB gene is a potent genetic marker, because of high heterozygocity (more than 80%). Previously, we found 16 different HVR alleles in the Belgrade population. The hypervariable region is located about l00 bp downstream of the second polyadenyl signal containing tandem repeats 30bp long. DNA was extracted from whole blood, dried blood spots or hairs. DNA fragments were synthesized using specific oligonucleotides and optimal conditions for Taq polymerase. Answers to the questions posed about DNA typing were known after 1,5% agarose gel electrophoresis. Considering both the human diploid chromosomal set and codominant inheritance of HVR alleles, it is a fast, easy and accurate identification in cases with a limited number of persons, for example relatives in the first and the second degree of kinship. The use of this polymorphism in forensic medicine for an accurate establishment of kinship and for other purposes is demonstrated, for example evaluation of the success of bone marrow transplantation, etc.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
APOLIPOPROTEIN B GENE DNA POLYMORPHISMS (EcoRI and MspI) AND SERUM LIPID LEVELS IN A SERBIAN NORMOLIPIDAEMIC POPULATION: INTERACTION OF RARE ALLELES AND SMOKING WITH CHOLESTEROL LEVELS
Sanja GLIŠIĆ, Ivana SAVIĆ i ALAVANTIĆ
Institut za nuklearne nauke "Vinča", Laboratorija za radiobiologiju i molekularnu genetiku, Beograd
The frequency of restriction fragment length polymorphisms (RFLPs) of the apolipoprotein B gene, detected by EcoRI and MspI, and their influence on serum lipids were studied in a total of 239 normolipidaemic subjects from the Belgrade area. The influence of the interaction between different genotypes and smoking was also studied. The relative frequency of both rare R2 and M2 alleles (lacking the cutting site) was similar to that reported in other groups of Caucasians (0.16 and 0.11 respectively). No association was observed between the apoB genotypes/haplotypes and serum lipid levels adjusted by age, BMI and blood pressure, either in the whole sample or in either women or men. When smokers and non-smokers were considered separately, smoking had a significant impact on total cholesterol variability in all individuals with genotype M1M2, and HDL cholesterol variability in women with genotype R1R2. It was shown that the presence of rare alleles of these two polymorphisms in smokers leads to a decrease of lipid levels, either in the whole sample or in both women and men analyzed separately, except for an increase of the HDL cholesterol level in men smokers, heterozygous for EcoRI polymorphism (R1R2).
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
MULTIALLELIC DNA POLYMORPHISM AT TH E 3' END OF THE apoB GENE (3'HVR) AND SERUM LIPID LEVELS IN NORMOLIPIDEMIC INDIVIDUALS
Sanja GLIŠIĆ, Ivana SAVIĆ i ALAVANTIĆ
Institut za nuklearne nauke "Vinča, Laboratorija za radiobiologiju i molekularnu genetiku, Beograd
The influence of the presence of various hypervariable region (HVR) alleles at the 3' end of the apoB gene, the main protein constituent of LDL, on serum lipid levels was studied. The sample from the Belgrade area included 293 normolipidemic persons (162 women and 131 men) chosen randomly. DNA was extracted from whole blood. A Polymerase Chain Reactin (PCR) method was performed for the amplification of the specific sequence, and reaction products were analyzed on agarose gel. Statistical analysis was made considering risk factors such as age, sex, smoking, blood pressure and body mass index. 16 different alleles whose sizes depend on the number of repeats (from 26 to 54) containing 15 predominantly A and T nucleotides were found. The most frequent alleles contained 34 and 36 repeats (34HVR and 36HVR). The association of the particular genotype and serum lipid variation was analyzed after the sample was divided into 6 groups depending on allele combinations which have an equal, higher or lower number of repeats than 36 (36HVR, > 36HVR AND < 36HVR). It was shown that DNA polymorphism of this region has a significant influence on LDL cholesterol variation in women. Also, women with genotype > 36HVR/ = 36HVR have the highest values of both total and LDL cholesterol, as well as triglycerides.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
AN INSERTION/DELETION POLYMORPHISM IN THE SIGNAL PEPTIDE OF THE apoB GENE
Sanja GLIŠIĆ, Ivana SAVIĆ, Nadežda RADOJA i ALAVANTIĆ
Institut za nuklearne nauke "Vinča", Laboratorija za radiobiologiju i molekularnu genetiku, Beograd.
The signal peptide of human apolipoprotein B (apoB) gene shows a polymorphism resulting in the variable peptide length. Because the signal peptide is involved in the translocation of apoB across the membrane of the endoplasmic reticulum, a loss of hydrophobic aminoacids could affect apoB translocation and quantity of lipoprotein particles. The purpose of our work on this polymorphism was: I. to detect Ins/Del polymorphism in the Belgrade population, the number of alleles and their frequencies, 2. to register an association between this polymorphism and the variation in lipid levels. DNA was extracted from whole blood of 237 normocholestcrolemic and 52 hypercholestcrolemic persons. A Polymerase Chain Reaction (PCR) with specific oligonucleotides (ID14'CAGCTGG CGATGGACCCGCCGA3' and ID25'ACCGGCCCTGGCGCCCGCCA GCA3') in optimal conditions for Taq polymerase in the presence of 10%DMSO resulted in two DNA fragments with different lengths. A longer fragment with 93bp, coding 27 aminoacids, named Ins (insertion) allele and a shorter one with a length of 84bp, coding 24 aminoacids, called Del (deletion) allele were found. Visualization of PCR products on 8% polyacrylamide gel after silver staining enabled the detection of 19.7-49,2% more heterozygote’s for the Ins/Del polymorphism tahn in all other normolipidaemic populations that have been studied. Also, the frequency of the Del alclle in the Belgrade population was higher than in other populations. However, statistical analysis (TC test) showed that there was no significant difference in frequencies of tch two alleles (Ins and Del) between the normolipidemic and hyperlipidemic population. There was a linear dependence of Ins allele frequencies among 6 groups of normocholesterolemic persons with ascending lipid values. This tendency was confirmed by a comparison with hypercholesterolemic individuals with high LDL-cholesterol levels.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
EXTRACELLULAR PROTEINASES OF MESOPHILIC LACTOBACILLI
Đ. FIRA, M. KOJIĆ, A. BANINA i Lj. TOPISIROVIĆ
Inslitut za molekularnu genetiku i genetičko inženjerstvo, Beograd.
Strains of mesophilic lactobacilli which produce extracellular proteinases are identified by screening a large collection of natural isolates. In order to determine the substrate specificity of these proteinases, their activity against major casein fractions (asi, β and κ-casein) has been tested. The majority of strains synthesize acid proteinases which predominantly hidrolyse - βcasein. The exception is Lactobacillus divergens 742, a strain which produces a proteinase that hydrolysis all three casein fractions, with the optimum at basic pH values. All proteinses tested are of the serine-type, since their action is inhibited by a specific inhibitor (phenyl methyl sulfonyl fluoride). It was shown in hybridization experiments that total DNA isolated from these strains in most of the cases hybridizes with probes from the lactococcal protcinase gene regions. On the basis of hybridization experiments, preliminary restriction maps of proteinase gene regions of several strains of mesophilic lactobacilli have been constructed, indicating the high level of homology with the lactococcal protcinase gene regions. Among strains which do not hybridize with the lactococcal proteinase probes, the strain Lactobacillus paracasci EN1 is very interesting since it produces exopolysacharide besides extracellular proteinase. The derivative of this strain, obtained by the plazmid curing treatment, besides loss of the capability of exopolysacharide synthesis, also showed increased proteolytic activity.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
THE NUCLEOTIDE SEQUENCE AND TRANSCRIPTIONAL ANALYSIS OF THE nov GENE WHICH AFFECTS THE RESPONSE OF E. coli K12 TO THE ANTIBIOTIC NOVOBIOCIN
Radmila V. IVANIŠEVIĆ, Mirjana M. MILIĆ, Dragana S. AJDIĆ, Milija Z. JOVIČIĆ, Mila J. JANKOVIĆ i J. SAVIĆ
Institut za molekularnu genetiku i genetičko inženjerstvo
The target of the coumarin group of antibiotics in E. coli is the enzyme DNA gyrase (GyrB subunit), which is involved in the control of bacterial DNA supercoiling. Most of the mutants conferring resistance to coumarins map within the gyrB gene (encoding for GyrB protein). In our laboratory the now gene was discovered that affects the response of E. coli K12 to novobiocin (Molecular Microbiology, 1992. 6: 1547-1553). The nucleotide sequence of the nov gene was determined. Nucleotide sequence analysis revealed the presence of an open reading frame 1461 base pairs long. Transcription is driven from the promotor sequence which is recognized by the subunit of RNA polymerase. Two A nucleotides (6 to 7 nucleotides away from the "40" sequence, respectively) were determined as transcriptional start sites.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
REPLICATION AND MUTAGENESIS: ANALYSIS OF A HIGHLY MUTABLE SEQUENCE FROM THE hisC GENE OF E. Coli
Mila J. JANKOVIĆ, Mirjana M. MILIĆ, Radmila V. IVANIŠEVIĆ, Z. JOVIČIĆ i J. SAVIĆ
Institut za molekularnu genetiku i genetičko inženjerstvo
Our previous studies showed that an E. coli strain deficient in DNA polymerase I is a very strong mutator creating mutations of exclusively minus frameshift and deletion types. The hotspot is located within the hisC gene, in the sequence consisting of four consecutive GCTG quadruplets (Mol Gen Genet, 1990. 223:481-486). The analysis of the sequence showed that it contains a four times repeated 3'GTC 5' triplet that is recognized by the E. coli primase. A possible relationship between DNA replication and a very high frequency of mutations arising at this site was analysed using fusions of the relevant fragment from the hisC gene with the lacZ gene, galactosidase is synthesized only if a particular frameshift mutation happens and the sequence recognized by the primase is located either in the leading or lagging strand during plasmid replication.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
lieS AND glnF (ipoN) MUTANTS OF E. coli SHOW AN INCREASED RESISTANCE TO NOVOBIOCIN
Z. JOVIČIĆ, Mirjana M. MILIĆ, Radmila V. IVANIŠEVIĆ, Mila J. JANKOVIĆ i D. SAVIĆ
Institut za molekularnu genetiku i genetičko inženjerstvo, Beograd.
Novoboicin is a coumarin antibiotic which inhibits the enzymatic activities of the GyrB subunit of the topoisomcrase II enzyme. Topoisomerase II is a bacterial enzyme responsible for converting circular DNA to a negatively supercoiled form. It is known that gyrB mutanats and some cysteine (cysB and cysE) mutants show increased levels of resistance to novobiocin (Mol Gen Genet, 1991. 228:307-311). We have isolated isoleucine and glutamine novobiocin resistant auxotrophs by direct selection on novobiocin plates. It has been demonstrated, by monitoring the efficiency of plating of parental strains and isoleucine and glutamine mutants that they do not exhibit resistance to other large hydrophobic antibiotics. Isoleucine and glutamine mutations were mapped by PI transduction experiments. Glutamine mutants carried mutations in the glnF (rpoN) gene and isoleucine mutants carried mutations in the lieS gene. The glnF (rpoN) gene codes the subunit of RNA polymerase and lieS gene codes isoleucyl-tRNA synthetase. Complementation of isoleucine mutants with a plasmid which carries the WT lieS gene has also shown that these mutations in the lieS gene are responsible for novobiocin resistance.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
THE EFFECT OF BIOANTIMUTAGENS ON INTRACHROMOSOMAL RECOMBINATION IN Escherichia coli K12
KNEŽEVIĆ-VUKČEVIĆ, B. ČULJKOVIĆ, M. VULOVIĆ, B. VUKOVIĆ-GAČIĆ i D. SIMIĆ
Katedra za mikrobiologiju, Institut za botaniku i Botanička bašta, Biološki fakultet, Univerzitet u Beogradu, Beograd
The main goal of the expanding research area of antimutagenesis/anticarcinogenesis is to detect inhibitors or modulators of environmental genotoxic agents and to elucidate the mechanisms of their action. There is already considerable evidence for the antimutagenic effect of certain naturally-occurring compounds affecting DNA repair and replication in bacteria and mammalian cells. Antimutagens act through different mechanisms: by chemical or enzymatic inactivation of the mutagen (desmutagens) or by interfering with cellular processes of the mutation fixation (bioantimutagens). The bacterial short-term tests, routinely used to detect environmental mutagens, are recommended for identifying antimutagens. By using the E. coli reversion assay system we have previously found that among differently prepared extracts of cultivated and wild typesage (Salvia officinalis L.) only the CO2, re-extract of cultivated sage, prepared without distillation of ethereal oils (Extract 1), specifically reduced the frequency of UV-induced mutations in the repair proficient strain. In this work we used a set of E. coli K12 strains with different alleles of the recA gene to test whether the bioantimutagenic effect of Extract 1 is due to the enhancement of error-free recombination repair. We measured intrachromosomal recombination between nonoverlapping deletions in the lac operon by monitoring lac recombinants on McConkey lactose plates with or without the plant extract. Two model bioantimutagens, coumarin and tannic acid which enhance recombination and excision repair, respectively, were used to validate the assay system. Extract 1 enhanced the formation of Iac+ recombinants in UV-irradiated or untreated recA+ and recA730 strains, suggesting that its antimutagenic effect is due to the stimulation of recombination.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
A MOLECULAR ANALYSIS OF A LARGE PLASMID pS50-290 IN Lactococcus lactis subsp. laclis biovar. Diacetylactis
M. KOJIĆ, Lj. TOPISIROVIĆ
Institut za molekularnu genetiku i genetičko inženjerstvo, Beograd.
Lactococcus lactis subsp. laclis biovar. diacetylactis was isolated from a buter starter culture. It was established that strain S50 is able to synthesize bacteriocin S50 with a narrow antibacterial spectrum. In addition, it synthesized a proteinase of the Pl-type. Genetic analysis showed that agents encoding proteins and bacteriocin production as well as resistance to bacteriocin are located on a large plasmid pS50-290 (290 kb). Besides this plasmid, strain S50 contained three smaller plasmids of which the smallest had a size of 7 kb (pS50-7). Plasmid pS50-7 is a cryptic one, since derivative S70-20, cured of this plasmid, retained the same growing characteristics as the original strain S50. Hybridization experiments (labelled pS50-7 was used as a probe) revealed that plasmid pS50-2909 contained a sequence homologous to that of pS50-7. In addition, homology between plasmid pS50-7 and a plasmid of approx. 20 kb, present in strain S50, was found. In the conjugation cross between donors S50 Rif, Bac+, Bacr and recipients MG7284 FusR, SpcR, BacS (selection for the resistance to bacteriocin S50 was applied), the transfer of plasmid pS50-290 most probably resulted in its resolution into two plasmids (approx. 283 kb and 7 kb) in transconjugant MGIO. Comparative restriction analysis of the 7 kb plasmid, isolated from transconjugant MGIO, and pS50-7 from the strain S50 showed that they have the same restriction pattern. To distinguish between plasmid cotransfcr (plasmids pS50-7 and pS50-290) and the assumed resolution, PFGE experiments were performed with Smal-digested DNA of the strain S50 and transconjugant MGIO. These experiments gave results which are in favour of plasmid pS5()-290 resolution during conjugation, since pS50-290 has two Smal sites, whereas a plasmid of approx 283 kb in transconjugant MGIO has one. The other Smal site was detected in the 7 kb plasmid of this transconjugant.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
REGULATION OF THE EXPRESSION OF THE sgm GENE FROM Micromonospora zionensis
KOJIĆ, TOPISIROVIĆ, Branka VASILJEVIĆ
Institut za molekularnu genetiku i genetičko inženjerstvo, Beograd.
The strain m. zionensis, producer of antibiotic G-52, has the sgm gene which encodes 16S rRNA methylase responsible for resistance to aminoglycoside antibiotics. This gene possesses complex transcriptional regulation from two promoters, PI and P2. These promoters were subcloned into the promoter-probe vector and it was shown that the P2 promoter had four time stronger activity. The existence of two promoters enables differential gene expression. It was suggested that promoter PI was responsible for the constitutive expression of the sgm gene, while the promoter P2 had a role in the expression of downstream (biosynthetic) gene. Apart from the transcriptional regulation, this gene was regulated at the translational level. In order to prove this, sgm-lacZ gene fusions were used. The level of - galactosidase activity was determined and it was shown that an extra copy of the sgm gene decreased the activity either in cis or in irons position. These results suggested that the expression of the sgm gene is regulated by the translational autorcprcssion due to binding of the methylase to its own mRNA. It was shown by computer analysis that the same hexanucleotide is present 14 bp in front of RBS and in the C-I400 region of 16S rRNA, i.e. the region where most of the aminoglycoside resistance methylases act. This complex mode of regulation of the sgm gene provides enough molecules of methylase to protect the cell from the action of its own antibiotic. On the other hand, if all ribosomes are modified, sgm methylase binds to its own mRNA preventing any further translation.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
RECOMBINANT DNA TECHNOLOGY IN PLANT VARIETY PATENTING AND PROTECTION OF AUTHORS RIGHTS
Kosana KONSTANTINOV, Snežana MLADENOVIĆ, Snezana GOŠIĆ, G. SARATLIĆ, N. DELIĆ, R. PETROVIĆ
Institut za kukuruz, Zemun Polje, Beograd-Zemun
The visible advantage of recombinant DNA technology is its application in plant variety patenting and protection of breeders' rights. This technology enables basic genetic information on the existing genome organization and the identification of specific genome regions in a particular plant genome. Molecular genetic markers such as izoenzymes and restriction fragment length polymorphism (RELP) and their combinations are a powerful tool for plant genotype identification. Gene identification, location, expression and linkages of genes are crucial in plant variety protection. Several gene probes (rDNA, ZE-22, C1, wx-8, wx-9) were used to screen numerous maize genotypes. The results obtained indicated that visible differences could be identified between the investigated inbred lines and hybrids of maize. A combination of izoenzyme pattern and gene location will result in the "plant identity card". Besides existing polymorphism in the plant genome, new genes, originating either from the same plant species or from different plant species, bacteria or viruses could be integrated through gene technology, expressed and inherited through plant generations. These new genes are at the same time genetic markers in genotype identification. The new approach of integration of synthetic oligonucleotides as genetic markers in gene flanking sequences will be presented.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
TEMPERATURE - DEPENDENT ACTIVITIES OF HYBRID Escherichia coli PROMOTERS
KONSTANTINOVIĆ, Sanja IVKOVIĆ i GLIŠIN
Institut za molekularnu genetiku i genetičko inženjerstvo, Beograd
We have studied the effect of temperature on the activities of two hybrid E. coli promoters tac (De Boer, 1982) and PLtl (Konstantinović et al. 1991), which have the same nucleotide sequence of -35, -10 and the transcription start point region, but differ in upstream sequences. One of the promoters, tac carries the sequence for lac I, whilst PHI contains the lalcl and cl repressors binding sites. Our results show that, depending on the temperature, chemically regulated tac and, chemically and thermally regulated PLtl promoter exhibited different levels but similar profiles of activities when fused to the lacZ reporter gene.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
THE ACTIVATION OF ONCOGENES IN HUMAN MYELOID NEOPLASTA
Koviljka KRTOLICA, Nadežda UROŠEVIĆ, Dobrila NEŠIĆ, Ljiljana TUKIĆ, Z. MAGIĆ i B. DIMITRIJEVIĆ
Laboratorija za radiobilologiju i
molekularnu genetiku, Institut za nuklearne
nauke "Vinča", Beograd, Institut za medicinska istraživanja i klinika
za
hematologiju Vojno-medicinske akademije, Beograd.
One of the three borad classes of genes, involved in the transition from a normal cell to a malignant one, are oncogenes. Ras genes are frequently found to harbor a mutation in human mycloid neoplasia. Our aim was to indentify oncogene activation by point mutation or by amplification in genomic DNA, isolated from human peripheral white blood cells of mycloid neoplasia. The PCR amplification and slot-blot hybridization techniques were used for the detection of point mutation of codons 12 or 13, of the N-ras or K-ras genes. Slot-blot or Southern blotting analysis were used for the detection of any amplification of genes: N-ras, K-ras, c-myc and N-myc. The analysis of 4 MDS, 23 AML and 11 CML patients, revealed the prevalence of N-ras mutations (83%) over K-ras mutations (17%). A five to tenfold amplification of the c-myc oncogene was revealed in 24% of AML patients, where half of them had one of the ras mutations. All MDS patients were harboring one of the mutations: either the amplification of c-myc, K-ras and N-ras oncogene or the point mutation in the codon 12 of the N-ras gene. Two of them harboured double mutations. These results suggest that the collaboration between genes, such as ras and myc, might play a significant role in the pathogenesis of some AML and MDS patients.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
GENOTYPE-SPECIFIC ACCUMULATION OF BAR17mRNA IN MAIZE LEAVES DURING DROUGHT STRESS
Vesna LAZIĆ-JANČIĆ, D. KOVAČEVIĆ, A. STEED i S.A. QUARRIE
Institut za kukuruz "Zemun Polje", Beograd-Zemun,
Cambridge Laboratory,AFRC-IPSR, JI Centre, Colney Lane, Norwich, NR4
Plant respond to drought stress by accumulating abscisic acid (ABA). Increased concentrations of ABA, in general, help the plants to adapt to the stress. An increasing number of RAB (Responsive to ABA) genes are known to be induced by drought. Using maize genotypes known to vary in their capacity to accumulate ABA in response to a rapid dehydration-treatment we have shown that accumulation of RAB17mRNA is significantly correlated with leaf ABA content. The threshold for significant accumulation of the RABlVmRNA was between 50 and 140 ng AB/g fresh weight. At a given level of ABA, genotypes also differed in the quantity of this mRNA species.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
INVESTIGATION ON QTL FOR ABSCISIC ACID PRODUCTION USING RFLP MARKERS
Vesna LAZIĆ-JANČIĆ, C. LEBRETON, A. STEED i S.A. QUARRIE
Institut za kukuruz "Zemun Polje", Beograd-Zemun,
La Gourjonnierc, 35680 BAIS, France,
Cambridge Laboratory, AFRC-IPSR, JI Centre, Colney Lane, Norwich NR4
Identification of target molecules involved in beneficial adaptive responses at the whole plant level is a goal of many research groups working on the improvement of plant adaptation to environmental stresses. A major biochemical response to a wide spectrum of abiotic stresses and especially to drought is the increased production of the hormone abscisic acid (ABA). In general, increased tissue ABA concentrations make the plant better adapted to water shortage. The accumulation of ABA by detached and partially dehydrated maize leaves is known to be inherited in a quantitative manner. The location of the genes having a major effect on ABA concentrations in maize was determined using an F2 population of 81 individuals produced from parental lines that differed markedly in ABA content under different types of stress treatments (Polj17, high ABA and F-2, low ABA). Preliminary information on the likely chromosomal location of genes affecting ABA accumulation was obtained using MAPMARKER QTL. About 80 RFLP markers have been mapped coverings every chromosome. Significant QTL for leaf and xylem sap ABA content were located on every chromosome except chromosome 8. The biggest QTL effects on the ABA content of stressed leaves were on chromosomes 2 and 3 (2 loci). These did not coincide with any of the QTL for xylem ABA content and only the QTL on chromosome 2 with leaf ABA content under non-stressed conditions. Three of the QTL (on chromosomes 1, 4 and 5) were associated with more ABA in plants with the F-2 allele.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
RADIATION INDUCED EXPRESSION OF ACUTE PHASE PROTEIN GENES IN RAT LIVER
Z. MAGIĆ, Svetlana MATIĆ i G. POZNANOVIĆ
Institut za medicinska istraživanja, VMA, Beograd
Institut za biološka istraživanja "Siniša Stanković", Beograd
It is well established that ionizing radiation can induce DNA damage (strand breaks, mutation and chromosome aberrations). However, during the last few years increasing data show that ionizing radiation can induce the expression of different genes in eukaryotic cells. It was shown that there exists a kind of cascade in gene activation and that genes which induce the acute-phase protein gene expression are included in this cascade. Acute-phase proteins are a group of plasma proteins with an important role during the general reaction of an organism to different kinds of trauma. Taking these facts into consideration we have measured changes in the concentrations of albumin (Al), fibrinogen (Fb), haptoglobin (Hp), cysteine inhibitor protease (CPI) and i-acid glycoprotein (AGP) in the plasma of irradiated rats (inbred Albino Oxford_AO strain, male rats) after total body irradiation with 12 Gy of gamma rays (absolutely lethal dose). In the liver of irradiated rats the relative concentrations of their mRNA were measured after dot-blot hybridization with appropriate plasmid cDNA probes (nick labelled with 32P-dCTP). The obtained results showed that total body radiation caused a significant increase of plasma concentrations for Hp and Fb (2-3 fold compared tot he control values) and especially for CPI and AGP (4-5 fold), while the Al concentration was not significantly changed. Changes in the relative concentrations of the liver mRNA for Al, Hp, CPI and Fb were in correlation with the observed changes of their protein concentrations in the plasma of irradiated rats. In the case of AGP the increased concentration of protein was not accompanied with an appropriate change in the relative concentration of AGP mRNA, indicating that the increased concentration of AGP after irradiation was not preceded by a proportional increase of the relative transcriptional rate. In conclusion, these results showed that an absolutely lethal dose of ionizing radiation activated an acute-phase protein gene expression in rat liver.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
ANALYSIS OF mRNAs IN DEVELOPING BUCKWHEAT SEEDS
Vesna MAKSIMOVIĆ, Svetlana RADOVIĆ, Erika VARKONJI i Ana SAVIĆ
Institut za molekularnu genetiku i genetičko inženjerstvo, Beograd.
Tissue-specific and developmentally regulated seed storage protein gene expression is one of the events characterizing higher plant embryogenesis. Buckwheat embryogenesis, usually lasting 30 days, can be divided into several stages, clearly morphologically distinctive. PolyA RNAs have been isolated from different stages and translated in vitro using a wheat germ extract containing 35S-mcthionine. Analysis of the in vitro translation products from different developmental stages by SDS PAGE indicated that storage protein mRNAs are detectable after the 9th day after flowering and their synthesis continues throughout the whole seed development. The existence of individual mRNAs for different polypeptides of the storage protein complex excludes the existence of precursor mRNA which has been found in the gene expression of other "legumin-like" storage proteins.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
THE ANEMIC BELGRADE RAT 67kD ERYTHROCYTE MEMBRANE PROTEIN
Jelena MARJANOVIĆ, V. GLIŠIN i Zvezdana POPOVIĆ
Inslitut za molekularnu genetiku i genetičko inženjerstvo, Beograd.
The Belgrade laboratory rat (b/b) suffers from hereditary microcytic, hypochromic anemia inherited as an autosomal trait. The mutation affects the iron transport across the cell membrane. SDS-PAGE analysis of b/b red blood cell (RBC) membrane proteins revealed an extra protein band with a 67kD molecular mass. The Western blot analysis demonstrated that the 67kD protein is present in b/b RBC membranes, only. The major protein in an exosome fraction of both b/b and control rats comigrated in SDS polyacrylamide gels with the 67kD protein band from b/b RBC membranes. Polyclonal antibodies raised against this protein from control exosomes cross reacted with the b/b 67kD protein band. Incubation of the b/b erythrocyte membrane emulsion in the presence of ATP, resulted in the elution of the 67kD protein band and an 80kD protein, presumably Protein 4.1. No such dissociation was seen in control membranes. Further analysis should confirm both the connection of the 67kD protein band with Protein 4.1 in the b/b erythrocyte membrane and its possible ATPase activity.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
ANALYSIS OF THE REGULATOR DOMAIN OF THE prt GENE IN Lactococcus lactis subsp. lactis BGIS29
N. MILADINOV, B. BOJOVIĆ, M. KOJIĆ, A. BANINA i Lj. TOPISIROVIĆ
Institut za molekularnu genetiku i genetičko inženjerstvo, Beograd.
Laciococcus lactis subsp. lactis BGIS29 was isolated from homemade cheese. It contains several cryptic plasmids. It appeared that some at the plasmids contained genes encoding resistance to the production of bacteriocin. In addition to bactericin, the strain BG1S29 produced an extracellular proteinase which proved to be a PI-lactococcal type of serine proteinase (hydrolyzed only -casein). This is an acid proteinase with an optimal temperature of 30C. Genetic analysis showed that the protcinase region, containing prlP and prtM genes, in the strain BGIS29 is located on a large plasmid of approx. 130 kb. It seems that the organization of genes within the proteinase region in the strain BGIS29 is very similar to that of L. lactis subsp. cremoris Wg2, since restriction maps of these regions are highly similar. The only apparent difference is that the regulator domain of proteinase genes in the strain BGIS29 contains no Clal sites, whereas the strain Wg2 contains two Clal sites, which flank the regulator domain. The regulator domain of the strain BGIS29 containing promoters of proteinase genes was enriched by PCR by using primers PO4C-P15 that originated from strain Wg2. The obtained PCR-enriched DNA fragment was cloned in vector pBV5030 and such a construct was used for the electroporetion of E. coli C600 and L. lactis 1L1403. Hybridization experiments confirmed that cloning was successful and that a low level of similarity exists between regulator domains of proteinase regions of strains BGIS29 and Wg2. This result is in agreement with the previously observed differences in restriction maps of these two strains. Testing of the minimal inhibitory concentration (MIC) of resulting transformants showed that the cloned promoter fragment expressed an activity in both E. coli and L. lactis IL1403. However, the cloned DNA fragment proved to be very unstable in the E. coli recA strain, suggesting that this fragment had been subjected to deletions and/or other genetic rearrangements.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
A NEW cysB REGULATED GENE THAT MODIFIES NOVOBIOCIN REZISTANCE IN cysB MUTANATS OF Escherichia coli
Mirjana M. MILIĆ, M.Z. JOVIČIĆ, Radmila V. IVANIŠEVIĆ, Mila J. JANKOVIĆ i J. SAVIĆ
Institut za molekularnu genetiku i genetičko inženjerstvo, Beograd.
The CysB protein is a positive regulator of gene expression for the cysteine regulon, a system of 11 genes whose products are involved in the transport of sulphate, reduction to sulphide and biosynthesis of cysteine. In our laboratory we have discovered that cysB and cysE mutants of E. coli show an increased resistnace to novobiocin, a hydrophobic antibiotic whose target in the cell is the enzyme gyrase (Jasna Rakonjac, Mirjana Milić and Dragutin Savić; Mol. Gen. Genet. 1991, 228:307-311). In this report we have described the discovery of a new gene. This gene is CysLB regulated but it is not involved in the biosynthesis of cystcine. We used the system of AplacMu phage which allows the construction of random gene fusions. We selected a gene fusion which was activated in the absence of the CysB protein but it was repressed in the presence of the CysB protein. The examination of cysB mutants with this fusion on (LA) plates with novobiocin indicated that this fusion decreased the resistance to novobiocin. Mapping by PI transduction and Southern hybridisation revealed that this gene is located in the vicinity of the cysB gene. The gene was cloned and a comparison of the nucleotide sequence with known genes from a data base confirmed that it is really a new gene in E. coli.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
THE INFLUENCE OF BACTERIAL ON MULTIPLE ALLELE POLIMORPHISM IN MAIZE
Snežana MLADENOVIĆ, Kosana KONSTANTINOV, Bojana TADIĆ i G. SARATLIĆ
Institut za kukuruz Zemun Polje, Beograd - Zemun
The maize genome was transformed by integration of bacterial gene into the maize genome using different technologies. The introduced gene expression (NPTII - neomycinphosphotransferase activity), gene integration (Southern blot analysis) and gene transmission were confirmed in T3 and TS transgenic plants. Besides phenotypic changes, eliminated through cell cycles, plant height alteration and reduction of the period between planting and flowering are inheritable traits and no segregation was obtained in T3 and T4 plants. The genotype at 16 izozyme loci was analysed in coleoptile tissue to correlate foreign gene integration and host genome expression. The profiles were changed in transgenic plants at several analysed loci: Acpi, Acp4, Mdhl, Mdh2, Mdh5, betaGlu, Idhf and Pgm. No alteration of isozyme Mdh3 and Mdh4 forms was obtained in any transformed plant. Transgenic plants kept both good general and specific combining abilities of the original maize line. The results obtained showed that bacterial gene integration could induce stable useful mutations which enable the identification and localization of the genes controlling particular traits of maize.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
AN ANALYSIS OF THE DISTAL PROMOTER OF THE RAT bminy - GLOBIN GENE
Sonja PAVLOVIĆ, Tatjana MITROVIĆ, GLIŠIN i Zvezdana POPOVIĆ
Institut za molekularnu genetiku i genetičko inženjerstvo, Beograd
The distal promoter located from -100 to -900 bp upstream from the first ATAG codon of the rat bminy -globin gene was cloned into the pUC19 vector in both directions. The sequence of the cloned fragment was determined using Sanger's dideoxy sequencing method. The sequence determination of this regulatory region allowed a computer search and comparison with the cis-acting sequences in other types of globin promoters analysed up to now.
Published in Book of Abstracts „THE FIRST CONGRESS OF SERBIAN GENETICISTS“
Vrnjačka Banja, Serbia, June 08-11, 1994
© 1994 Serbian Genetics Society
INVESTIGATION THE REGULATION OF THE ACTIVITY OF THE Escherichia coli PENICILLIN G AMIDASE GENE BY FUSIONS WITH DIVERGENTE REPORTER GENES
S. RADOJA, M. KONSTANTINOVIĆ i V.
GLIŠIN
Institut za molekularnu genetiku i genetičko inženjerstvo, Beograd
An investigation of the primary structure and function of Escherichia coli penicillin G amidase (pac) gene has indicated the existence of complex regulation of the synthesis of this enzyme. The results which show that in the proximity of the promoter region of the gene there is antisense mRNA i